XY sex determination in a cnidarian.

BACKGROUND: Sex determination occurs across animal species, but most of our knowledge about its mechanisms comes from only a handful of bilaterian taxa. This limits our ability to infer the evolutionary history of sex determination within animals. RESULTS: In this study, we generated a linkage map of the genome of the colonial cnidarian Hydractinia symbiolongicarpus and used it to demonstrate that this species has an XX/XY sex determination system. We demonstrate that the X and Y chromosomes have pseudoautosomal and non-recombining regions. We then use the linkage map and a method based on the depth of sequencing coverage to identify genes encoded in the non-recombining region and show that many of them have male gonad-specific expression. In addition, we demonstrate that recombination rates are enhanced in the female genome and that the haploid chromosome number in Hydractinia is n = 15. CONCLUSIONS: These findings establish Hydractinia as a tractable non-bilaterian model system for the study of sex determination and the evolution of sex chromosomes.

A Method of Planning Disaster Emergency Rescue Paths in Road-Free Environment.

The occurrence of natural disasters has caused great loss of lives and properties that affected many people. To improve the efficiency of the emergency rescue efforts, the problem of road planning for emergency workers, vehicles, and other mobile objects should be overcome. Due to the characteristics of the emergency planning for rescue paths, timely, effective, and rapid efforts should be taken into effect based on geographical data in the road-free environment. This paper analyzes trafficability, researches both access ability and traffic efficiency, and uses traffic efficiency concerning the road-free environment as an influential weight and timeliness to improve the A∗ algorithm, which realizes the rapid planning of the mobile objects' emergency rescue paths in the road-free environment, providing decision-making services for the rescue path planning of disaster emergencies in the environment of no road network. Finally, the experiments were carried out using available data for Shuozhou City, Shanxi Province. The results show that the research can provide a reference for the rescue path planning of disaster emergencies and has contributed to theoretical and applicable aspects of the research.

Combined PD-1/PD-L1 and tumor-infiltrating immune cells redefined a unique molecular subtype of high-grade serous ovarian carcinoma.

BACKGROUND: High-grade serous ovarian carcinoma is highly heterogeneous, and although many studies have been conducted to identify high-grade serous ovarian carcinoma molecular subtypes that are sensitive to immunotherapy, no precise molecular subtype has been proposed to date. Immune cell infiltration and immune checkpoints are highly correlated with immunotherapy. Here, we investigated immune cell infiltration and immune checkpoint values for prognosis and precise immunotherapy for high-grade serous ovarian carcinoma based on molecular subtype classification. RESULTS: "High antigen-presenting cells infiltration molecular subtype of high-grade serous ovarian carcinoma" was identified in immune cell infiltration profiles. Each of the three immune cell infiltration clusters (A, B, and C) demonstrated distinct immune cell characterization, with immune cell infiltration cluster C exhibiting high antigen-presenting cell infiltration, improved prognosis, and higher sensitivity to immunotherapy. Programmed death-1/programmed death ligand 1 has a prognostic and predictive role that can help classify molecular subtypes. CONCLUSIONS: Our findings redefined a unique molecular subtype of high-grade serous ovarian carcinoma, suggesting that high-grade serous ovarian carcinoma patients with higher antigen-presenting cell infiltration and programmed death-1/programmed death ligand 1 expression can benefit from precise immunotherapy.