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Nan Fang Yi Ke Da Xue Xue Bao
; 28(6): 1104-5, 2008 Jun.
Artículo
en Chino
| MEDLINE
| ID: mdl-18583274
RESUMEN
OBJECTIVE: To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma. METHODS: Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min. RESULTS: The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%. CONCLUSION: The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.