RESUMEN
The use of an extracranial-intracranial (EC-IC) bypass has been a choice to improve the safety of parent vessel occlusion during the management of aneurysm. However, the prognosis and subsequent patency of bypass graft are variable and have seldom been managed by endovascular treatment. A 38-year-old gentleman presented to our hospital with intermittent headache. Subarachnoid hemorrhage caused by an internal carotid artery aneurysm was disclosed on the subsequent examination. He received an EC-IC bypass later. However, graft stenosis was found during follow-up. To solve the stenosis, an endovascular stent was inserted by us. There is seldom report of endovascular treatment of the graft. Here we share our experience under such circumstances.
Asunto(s)
Revascularización Cerebral/métodos , Procedimientos Endovasculares/métodos , Aneurisma Intracraneal/cirugía , Stents , Adulto , Enfermedades de las Arterias Carótidas/complicaciones , Enfermedades de las Arterias Carótidas/cirugía , Revascularización Cerebral/efectos adversos , Constricción Patológica , Humanos , Aneurisma Intracraneal/complicaciones , Masculino , Reoperación , Hemorragia Subaracnoidea/etiología , Hemorragia Subaracnoidea/cirugíaRESUMEN
BACKGROUND: The function of microRNAs (miRNAs) depends on the binding of miRNAs to their target sequences in the 3'UTR of messenger RNAs (mRNAs), which enhances the degradation of mRNAs and consequently, represses their expression. Single nucleotide polymorphisms (SNPs) in the miRNA target sequences may affect or impair the binding of miRNAs. Studies have shown that SNPs in miRNA target sites (miR-TS-SNPs) have a great influence on diverse biological functions, including pharmacogenomics and disease susceptibilities in human. METHODS: High-resolution melting (HRM) analysis was applied for investigating the allele frequencies of 3 miR-TS-SNPs (PLA2G2A, IL-16, and NOD2) in acute leukemia. We also compared the genotypes of acute lymphoblastic leukemia patients at initial diagnosis and complete remission. RESULTS: HRM analysis revealed 3 genotypes (both homozygous and heterozygous) in the 3 miR-TS-SNPs. The allele frequencies of all 3 miR-TS-SNPs were similar in normal individuals and patients with acute myelogenous leukemia. Most patients with acute lymphoblastic leukemia had the same genotypes at initial diagnosis and complete remission. CONCLUSIONS: Large scale scanning of case-control studies for miR-TS-SNPs may contribute to the investigation of their roles and pathogenesis mechanisms in human diseases. Our study showed that HRM analysis can be an efficient tool for studies of miR-TS-SNPs.
Asunto(s)
Análisis Mutacional de ADN/métodos , MicroARNs/genética , Polimorfismo de Nucleótido Simple/genética , Alelos , Estudios de Casos y Controles , Genotipo , Humanos , Desnaturalización de Ácido Nucleico , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Leucemia-Linfoma Linfoblástico de Células Precursoras/genéticaRESUMEN
Epigenetic effects are considered heritable but may also be modified by environmental factors. Arecoline (ARC), a major component of areca nut alkaloids, is an important environmental risk factor for oral cancer and hepatocellular carcinomain Taiwan. The aim of this study was to determine the influence of ARC on the epigenome. The mRNA expression of histone methyltransferases, acetyltransferases, and demethylases were assessed in K-562 cells following exposure to ARC. Results demonstrated that ARC produced changes in the expressions of several genes catalyzing histone methylation (Mll, Setdb1, and Suv39h2), acetylation (Atf2), and demethylation (JMJD6). Since H3K9 methylation is involved in maintaining the stability of heterochromatin structures and inactivating euchromatic gene expressions, data suggest that the ARC-induced epigenetic changes play a role in the mechanisms underlying chemical-mediated cytotoxicity and genotoxicity.