Tree growth and density enhanced, while diversity and spatial clustering reduced soil mycorrhizal C and N sequestration: Strong interaction with soil properties in northeastern China.

In this paper, the effects of species diversity, tree growth, and spatial clustering on mycorrhizal carbon and nitrogen sequestration and the interaction of soil physicochemical properties in Northeast China were investigated. Based on 720 10 m ∗ 10 m plots in Harbin Experimental Forest Farm of Northeast Forestry University, we determined mycorrhizal biomarkers of easily extractable Glomalin-related soil protein (EEG) and total Glomalin-related soil protein (TG). Four plant diversity indices, seven structural metrics, and five soil properties were also measured. We found that: 1) The low tree diversity plots had 1.08-1.23 times higher TG, EEG, TG-N/TN (proportion of N in TG to TN), and TG-C/SOC (proportion of C in TG to SOC) than the high plots. 2) Tree diameter was negatively correlated with EEG and TG, but positively correlated with the EEG and TG contribution to soil TN and SOC. Soil EEG and TG were positively correlated with under-branch height and tree density. W (Uniform Angle Index, higher W indicates more clustering of tree distribution in the plot) was negatively correlated with the above four ratios and positively correlated with EEG/TG. 3) pH was the most powerful explainer for the GRSP variations (6.8 %, strongest negative association with GRSP/TN, R2 > 0.13), followed by soil electrical conductance (6.5 %, positive relation with TG, p < 0.05), AP (3.2 %). 4) Plant diversity mainly affected GRSP traits through the interaction with soils (0.07), tree growth and density directly increased TG, TG-N/TN, and TG-C/SOC, while tree spatial distribution directly reduced TG-N/TN. Our finding highlighted the important effects of tree diversity and forest structural traits on GRSP amount, carbon sequestration, and nutrient retentions, and could support glomalin-related forest soil management of temperate forests in the high-latitude northern hemisphere.

Tree diversity, growth status, and spatial distribution affected soil N availability and N2O efflux: Interaction with soil physiochemical properties.

Soil nitrogen (N) is an essential nutrient for tree growth, and excessive N is a source of pollution. This paper aims to define the effects of plant diversity and forest structure on various aspects of soil N cycling. Herein, we collected soils from 720 plots to measure total N content (TN), alkali-hydrolyzed N (AN), nitrate N (NO3--N), ammonium N (NH4+-N) in a 7.2 ha experimental forest in northeast China. Four plant diversity indices, seven structural metrics, four soil properties, and in situ N2O efflux were also measured. We found that: 1) high tree diversity had 1.3-1.4-fold NO3--N, 1.1-fold NH4+-N, and 1.5-1.8-fold N2O efflux (p < 0.05). 2) Tree growth decreased soil TN, AN, and NO3--N by more than 13%, and tree mixing and un-uniform distribution increased TN, AN, and NH4+-N by 11-22%. 3) Soil organic carbon (SOC) explained 34.3% of the N variations, followed by soil water content (1.5%), tree diameter (1.5%) and pH (1%), and soil bulk density (0.5%). SOC had the most robust linear relations to TN (R2 = 0.59) and AN (R2 = 0.5). 4) The partial least squares path model revealed that the tree diversity directly increased NO3--N, NH4+-N, and N2O efflux, and they were strengthened indirectly from soil properties by 1%-4%. The effects of tree size-density (-0.24) and spatial structure (0.16) were mainly achieved via their soil interaction and thus indirectly decreased NH4+-N, AN, and TN. Overall, high tree diversity forests improved soil N availability and N2O efflux, and un-uniform spatial tree assemblages could partially balance the soil N consumed by tree growth. Our data support soil N management in high northern hemisphere temperate forests from tree diversity and forest structural regulations.

Big-sized trees and higher species diversity improve water holding capacities of forests in northeast China.

High water-holding forests are essential for adapting to drought climates under global warming, and a central issue is which type of forests could conserve more water in the ecosystem. This paper explores how forest structure, plant diversity, and soil physics impact forest water-holding capacities. We investigated 720 sampling plots by measuring water-holding capacities from 1440 soil and litter samples, 8400 leaves, and 1680 branches and surveying 18,054 trees in total (28 species). Water-holding capacities were measured as four soil indices (Maxwc, maximum water-holding capacity; Fcwc, field water-holding capacity; Cpwc, soil capillary water-holding capacity; Ncpwc, non-capillary water-holding capacity), two litter metrics (Maxwcl, maximum water-holding capacity of litters; Ewcl, effective water-holding capacity of litters), and canopy interception (C, the sum of estimated water interception of all branches and leaves of all tree species in the plot). We found that water-holding capacity in the big-sized tree plots was 4-25 % higher in the litters, 54-64 % in the canopy, and 6-37 % in the soils than in the small-sized plots. The higher species richness increased all soil water-holding capacities compared to the lowest richness plot. Higher Simpson and Shannon-Wiener plots had 10-27 % higher Ewcl and C than the lowest plots. Bulk density had the strongest negative relations with Maxwc, Cpwc, and Fcwc, whereas field soil water content positively affected them. Soil physics, forest structure, and plant diversity explained 90.5 %, 5.9 %, and 0.2 % of the water-holding variation, respectively. Tree sizes increased C, Ncpwc, Ewcl directly (p < 0.05), and richness increased Ewcl directly (p < 0.05). However, the direct effects from the uniform angle index (tree distribution evenness) were balanced by their indirect effect from soil physics. Our findings highlighted that the mixed forests with big-sized trees and rich species could effectively improve the water-holding capacities of the ecosystem.