Effects of zero-valent iron (ZVI) on nitrogen conversion, transformation of sulfamethoxazole (SMX) and abundance of antibiotic resistance genes (ARGs) in aerobic granular sludge process.

Even after pre-treatment, livestock and poultry wastewater still contain high concentrations of ammonia and residual antibiotics. These could be removed economically using the aerobic granular sludge (AGS) process with zero-valent iron (ZVI). The interaction of antibiotics and nitrogen in this process needs to be clarified and controlled, however, to achieve good removal performance. Otherwise, antibiotics might generate transformation products (TPs) with higher toxicity and lead to the emergence of antibiotic-resistant bacteria carrying antibiotic resistance genes (ARGs), which could cause persistent toxicity and the risk of disease transmission to the ecological environment. This study investigated the impact of ZVI on AGS for nitrogen and sulfamethoxazole (SMX) removal. The results show that AGS could maintain good ammonia removal performance and that the existence of SMX had a negative impact on ammonia oxidation activities. ZVI contributed to an increase in the abundance of nitrite oxidation bacteria, denitrifying bacteria and the functional genes of nitrogen removal. This led to better total nitrogen removal and a decrease in N2O emission. Accompanied by biological nitrogen transformation, SMX could be transformed into 14 TPs through five pathways. ZVI has the potential to enhance transformation pathways with TPs of lower ecotoxicity, thereby reducing the acute and chronic toxicity of the effluent. Unfortunately, ZVI might enhance the abundance of sul1, sul2, and sul3 in AGS, which increases the risk of sulfonamide antibiotic resistance. In AGS, Opitutaceae, Xanthomonas, Spartobacteria and Mesorhizobium were potential hosts for ARGs. This study provides theoretical references for the interaction of typical antibiotics and nitrogen in the biological treatment process of wastewater and bioremediation of natural water bodies.

Effect of ozone pretreatment on biogranulation with partial nitritation - Anammox two stages for nitrogen removal from mature landfill leachate.

Due to the extremely low C/N ratio, high concentration of ammonia nitrogen and refractory organic matter of mature landfill leachate (MLL), appropriate processes should be selected to effectively remove nitrogen and reduce disposal costs. Partial nitritation (PN) and anaerobic ammonia oxidation (AMX) have been used as the main nitrogen removal processes for MLL, and the sludge granulation in PN and AMX processes could contribute to high biological activity, good sedimentation performance, and stable resistance to toxicity. In this study, the O3-PN-AMX biogranules process was selected to effectively remove nitrogen from MLL without carbon addition and pH adjustment. Without uneconomical NH4+-N oxidation and wasting the alkalinity of MLL, ozone pretreatment achieved color removal, decreased humic- and fulvic-like acid substances, and alleviated the MLL toxicity on ammonia oxidizers. In addition, the ozonation of MLL could shorten the start-up time and improve the treatment efficiency and biogranules stability of PN and AMX processes. Efficient and stable nitritation was achieved in PN reactor without strict dissolved oxygen (DO) control, which was attributed to the unique structure of granular sludge, ozone pretreatment, and alternating inhibition of free ammonia and free nitric acid on nitrite oxidizers. Through the application of ozone pretreatment and granular sludge, the nitrogen removal rate (NRR) and nitrogen removal efficiency (NRE) of the O3-PN-AMX biogranules process reached 0.39 kg/m3/day and 85%, respectively, for the undiluted MLL treatment. This study might provide a novel and effective operation strategy of combined process for the efficient, economical, and stable nitrogen removal from MLL.