RESUMEN
Three previously undescribed highly modified lanostane triterpenoids, ganopyrone A, ganocolossusin I, and ganodermalactone Y, were isolated from the artificially cultivated fruiting bodies of the basidiomycete Ganoderma colossus TBRC-BCC 17711. Ganopyrone A possesses an unprecedented polycyclic carbon skeleton with an α-pyrone ring and C-18/C-23 bond. It showed antimalarial activity against Plasmodium falciparum K1 (multidrug-resistant strain) with an IC50 value of 7.8 µM (positive control: dihydroartemisinin, IC50 1.4 nM), while its cytotoxicity (Vero cells) was much weaker (IC50 103 µM).
Asunto(s)
Antimaláricos , Cuerpos Fructíferos de los Hongos , Ganoderma , Plasmodium falciparum , Triterpenos , Ganoderma/química , Antimaláricos/farmacología , Antimaláricos/química , Antimaláricos/aislamiento & purificación , Plasmodium falciparum/efectos de los fármacos , Cuerpos Fructíferos de los Hongos/química , Triterpenos/farmacología , Triterpenos/química , Triterpenos/aislamiento & purificación , Animales , Estructura Molecular , Células Vero , Chlorocebus aethiops , Lanosterol/análogos & derivados , Lanosterol/farmacología , Lanosterol/química , Lanosterol/aislamiento & purificación , Pruebas de Sensibilidad Parasitaria , Relación Estructura-Actividad , Relación Dosis-Respuesta a DrogaRESUMEN
Five undescribed polyketide metabolites, oudemansins E (1), M (2), P (3), and Q (4), and 9-methoxystrobilurin I (5), were isolated from cultures of basidiomycete Favolaschia minutissima TBRC-BCC 19434. A γ-lactone derivative (6) of noroudemansin A (8), which was previously reported as a semisynthetic compound, was also isolated. The absolute configuration of the isoprene-derived moiety of the known cometabolite 9-methoxystrobilurin E (9) was determined to be 2'R,6'S by comparison of the experimental and calculated ECD data, which was correlated to the new derivative 1. These compounds exhibited antimalarial activity against Plasmodium falciparum K1 (multidrug-resistant strain). A putative minor natural product, namely 9-methoxystrobilurin P (13), was prepared by semisynthesis, which exhibited significant antimalarial activity (IC50 0.086 µM).
Asunto(s)
Antimaláricos , Basidiomycota , Antimaláricos/farmacología , Butadienos , Plasmodium falciparumRESUMEN
Seven undescribed compounds, including four naphthoquinone terpenoids (aculeolatins A - D), one rare 2-nitropyrrole terpenoid (nitropyrrolin F), and two hydroxamate siderophores (aculeolamides A and B) and one further undescribed compound (2,5,7-trihydroxy-3,6-dimethylnaphthalene-1,4-dione), together with eleven known compounds (arromycin, phenaziterpene A, nitropyrrolin A, heronapyrroles A and B, salaceyin A, 5,7-dihydroxy-2-isopropylchromone, 1-hydroxyphenazine, 1-methoxyphenazine, 1-acetyl-ß-carboline, and N-(2-phenylethyl) acetamide), were isolated from the cultures of the endophytic Streptomyces aculeolatus MS1-6. The structures of the isolated compounds were determined using NMR spectroscopy and corroborated using chemical modification. These compounds exhibited a broad spectrum of biological activities, including antimalarial (IC50 6.03-9.84 µg/mL), antitubercular (MIC 3.13-6.25 µg/mL), anti-plant pathogenic fungal (MIC 25.0-50.0 µg/mL), and antibacterial (MIC 3.03-50 µg/mL) activities; however, they displayed unremarkable cytotoxicity against cancerous (MCF-7 and NCI-H187) and non-cancerous (Vero) cell lines.
Asunto(s)
Actinobacteria , Antiinfecciosos , Antimaláricos , Antimaláricos/química , Antiinfecciosos/química , Antibacterianos/química , Antituberculosos , Pruebas de Sensibilidad MicrobianaRESUMEN
Two new hydroquinones bearing a 1,3-enyne moiety, pestalotioquinols G and H, together with four known compounds, including pestalotioquinol A, phomonitroester, (R)-4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one, and scylatone were isolated from the marine fungus Pestalotiopsis diploclisia (BCC 35283). The structures of these compounds were elucidated by analysis of 2D-NMR and HR-MS data. The known pestalotioquinol A displayed antimalarial activity against Plasmodium falciparum K1 with an IC50 value of 19.0 µM, while pestalotioquinol G displayed weak cytotoxic activity against Vero cell lines with an IC50 value of 47.9 µM.
Asunto(s)
Hongos , Hidroquinonas , Estructura Molecular , Hongos/química , Pestalotiopsis , PlantasRESUMEN
A new benzothioate glycoside metabolite, phitsanoside A, together with its new and known derivatives were isolated from Streptomyces sp. TBRC 11511 collected from sediment of a dry evergreen forest located in Phitsanulok Province, Thailand. The structure elucidation of the new compound was interpreted on the basis of spectroscopic data analysis. The configuration of the sugar moiety was derived based on NOESY nuclear magnetic resonance correlations, a vicinal coupling constant analysis, and the measurement of an optical rotation from the hydrolyzed sugar unit, which was identified as ß-D-glucopyranose. Phitsanoside A did not exhibit antibacterial activity against Bacillus cereus, Mycobacterium tuberculosis or Staphylococcus aureus, but phitsanoside B showed activity against all of them with MIC values of 3.13, 25 and 12.5 µg ml-1, respectively.
Asunto(s)
Streptomyces , Antibacterianos/química , Glicósidos/química , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Streptomyces/metabolismo , Azúcares , TailandiaRESUMEN
In the quest for medicinally active compounds in mushrooms of the genus Ganoderma, eleven undescribed lanostane triterpenoids, including a novel chlorinated derivative, i.e., (20S,24E)-21-chloro-15ß,20,29-trihydroxy-3,7,11-trioxolanosta-8,24-dien-26-oic acid, were isolated from artificially cultivated fruiting bodies of the basidiomycete Ganoderma mbrekobenum. The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry data. The configuration of the C-20 atom in the most abundant 20-hydroxy-lanostane, (20S,24E)-15ß,20,29-trihydroxy-3,7,11-trioxolanosta-8,24-dien-26-oic acid, was established by chemical derivatization, and the absolute configuration of the lanostane skeleton was determined by ECD calculation. Two of the undescribed compounds exhibited moderate antimalarial activity.
Asunto(s)
Ganoderma , Triterpenos , Cuerpos Fructíferos de los Hongos/química , Ganoderma/química , Estructura Molecular , Triterpenos/químicaRESUMEN
Eight new angucyclic quinones, miaosporones A to H (1-8), along with the previously described metabolites 8-hydroxy-3-methylbenz[a]anthraquinone (9), tetrangulol (10), 5,6-dihydro-1,8-dihydroxy-3-methybenz[a]anthracene-7,12-quinone (11), and SF2315A (12), were isolated from the terrestrial actinomycete Actinomadura miaoliensis TBRC 5172 obtained from sediment collected from the Huai Yang reservoir, Prachuap Khiri Khan Province, Thailand. The relative and absolute configurations of the new compounds were determined from analysis of NMR spectroscopic and X-ray crystallographic data. Miaosporone A exhibited antimalarial activity against Plasmodium falciparum K1 and antibacterial activity against Mycobacterium tuberculosis with respective IC50 values of 2.5 and 2.4 µM and displayed cytotoxic activities against both cancerous (MCF-7 and NCI-H187) and nonmalignant (Vero) cells.
Asunto(s)
Actinomadura/metabolismo , Antiinfecciosos/aislamiento & purificación , Antineoplásicos/aislamiento & purificación , Quinolonas/aislamiento & purificación , Antiinfecciosos/farmacología , Antineoplásicos/farmacología , Línea Celular Tumoral , Humanos , Espectroscopía de Resonancia Magnética , Mycobacterium tuberculosis/efectos de los fármacos , Plasmodium falciparum/efectos de los fármacos , Quinolonas/química , Quinolonas/farmacologíaRESUMEN
Thirteen tetrahydroxanthone dimers, atrop-ascherxanthone A (1), ascherxanthones C-G (2-6), and confluxanthones A-G (7-13), were isolated from the entomopathogenic fungus Aschersonia confluens BCC53152. The chemical structures were determined based on analysis of NMR spectroscopic and mass spectrometric data. The absolute configurations of compounds 1 and 7 were confirmed by single-crystal X-ray diffraction experiments, while the configurations of other compounds were assigned based upon evidence from NOESY and NOEDIFF experiments, modified Mosher's method, and ECD spectroscopic data together with biogenetic considerations. Compounds 1, 3-5, 7-11, and 13 showed antimalarial activity against Plasmodium falciparum (K1, multidrug-resistant strain) (IC50 0.6-6.1 µM), antitubercular activity against Mycobacterium tuberculosis H37Ra (MIC 6.3-25.0 µg/mL), and cytotoxicity against NCI-H187 (IC50 0.5-3.5 µM) and Vero (IC50 0.9-6.1 µM) cells. All tested compounds except for compound 9 exhibited cytotoxicity against KB cells (IC50 1.3-9.7 µM).
Asunto(s)
Antimaláricos/farmacología , Antituberculosos/farmacología , Hypocreales/química , Xantonas/farmacología , Animales , Antimaláricos/aislamiento & purificación , Antituberculosos/aislamiento & purificación , Chlorocebus aethiops , Células HeLa , Humanos , Estructura Molecular , Mycobacterium tuberculosis/efectos de los fármacos , Plasmodium falciparum/efectos de los fármacos , Tailandia , Células Vero , Xantonas/aislamiento & purificaciónRESUMEN
Two unknown enantiomeric compounds, named (R)- and (S)-taeniolin, along with six known compounds, were isolated from the marine-associated fungus Taeniolella sp. BCC31839. Chemical structures were determined by NMR spectroscopic techniques, and the absolute configurations were confirmed by Mosher application together with CD spectral analyses. Both were inactive for antimicrobial activity against multidrug-resistant malaria parasite (Plasmodium falciparum) and bacteria (Mycobacerium tuberculosis and Bacillus cereus) at maximum tested concentration.
Asunto(s)
Antibacterianos/farmacología , Antimaláricos/farmacología , Cromonas/química , Hongos Mitospóricos/química , Animales , Antibacterianos/química , Antimaláricos/química , Antineoplásicos/química , Antineoplásicos/farmacología , Bacillus cereus/efectos de los fármacos , Chlorocebus aethiops , Cromonas/farmacología , Dicroismo Circular , Evaluación Preclínica de Medicamentos , Humanos , Células MCF-7 , Espectroscopía de Resonancia Magnética , Hongos Mitospóricos/aislamiento & purificación , Estructura Molecular , Mycobacterium tuberculosis/efectos de los fármacos , Plasmodium falciparum/efectos de los fármacos , Células VeroRESUMEN
Ten previously undescribed lanostane-type triterpenoids (1-10), together with 15 known lanostanes, were isolated from artificially cultivated fruiting bodies of the basidiomycete Ganoderma sp. BCC 21329. The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry data and by application of the modified Mosher's method. Compounds 1, 3, 5, and 7 showed moderate antimalarial activity (IC50 3.8-7.6 µg ml-1).
Asunto(s)
Antimaláricos/aislamiento & purificación , Cuerpos Fructíferos de los Hongos/química , Ganoderma/química , Triterpenos/aislamiento & purificación , Antimaláricos/química , Antimaláricos/farmacología , Espectroscopía de Resonancia Magnética , Estructura Molecular , Plasmodium falciparum/efectos de los fármacos , Relación Estructura-Actividad , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
Six new compounds [ascherlactones A (1) and B (2), ascherchromanone A (3), phenethyl 4'-O-methylglucoside (8), 4'-O-methylpleoside (10), and 4'-O-methyltorachrysone 8-O-glucoside (11)] and one naturally new compound [4'-O-methyl-ß-d-benzylglucoside (9)] together with fourteen known compounds, including paecilodepsipeptides A (5), B (7), and D (4), conoideocrellide A (6), eugenin (12), 5-hydroxy-2,3-dimethyl-7-methoxychromone (13), (S)-1-phenyl-1,2-ethanediol (14), (2S)-l-3-phenyllactic acid (15), papuline [or (2S)-l-3-phenyllactic acid methyl ester, 16], 2'-epi terpendole A (17), terpendoles C (18) and D (19), cholic acid, and zeorin were isolated from the entomopathogenic fungus Aschersonia confluens BCC53152. Their chemical structures were elucidated on the basis of NMR spectroscopic and mass spectrometric analyses. The absolute configurations were determined by using the evidence from NOESY correlations, chemical means, optical rotation values together with comparison of ECD spectroscopic data with the calculated ECD spectra. The plausible biosynthetic pathway of compounds 1-3 was also proposed. Moreover, antimicrobial activity such as antimalarial, antitubercular, antifungal, and antibacterial activities and cytotoxicity against cancerous (MCF-7, KB, and NCI-H187) and non-cancerous (Vero) cells of the isolated compounds were evaluated.
Asunto(s)
Cromonas/farmacología , Glucósidos/farmacología , Hypocreales/química , Animales , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Antimaláricos/aislamiento & purificación , Antimaláricos/farmacología , Antineoplásicos/aislamiento & purificación , Chlorocebus aethiops , Cromonas/aislamiento & purificación , Glucósidos/aislamiento & purificación , Hemípteros/microbiología , Humanos , Células MCF-7 , Estructura Molecular , Tailandia , Células VeroRESUMEN
Fourteen new compounds, oudemansins 1-4, oudemansinols 5-7, favolasins 8-10, favolasinin (12), polyketides 13-15, and (R,E)-2,4-dimethyl-5-phenyl-4-pentene-2,3-diol (16), together with nine known compounds were isolated from the basidiomycete fungus Favolaschia sp. BCC 18686. Two new compounds, favolasin E (11) and 9-oxostrobilurin E (17), were isolated from the closely related organism Favolaschia calocera BCC 36684 along with nine ß-methoxyacrylate-type derivatives. Compounds in the class of oudemansins and strobilurins exhibited moderate to strong antimalarial activity with relatively low cytotoxicity against Vero cells (African green monkey kidney fibroblasts). Potent antimalarial activity was demonstrated for 9-methoxystrobilurins G, K, and E (IC50 values 0.061, 0.089, and 0.14 µM, respectively). The structure-activity relationships (SAR) for antimalarial activity is proposed on the basis of the activity of the new and several known ß-methoxyacrylate derivatives in combination with the data from previously isolated compounds. Furthermore, several compounds showed specific cytotoxicity against NCI-187 cells (human small-cell lung cancer), although the SAR was different from that for antimalarial activity.
Asunto(s)
Agaricales/química , Antimaláricos/química , Antimaláricos/farmacología , Policétidos/química , Policétidos/farmacología , Estrobilurinas/química , Estrobilurinas/farmacología , Acrilatos/química , Acrilatos/farmacología , Animales , Antibióticos Antineoplásicos/farmacología , Línea Celular Tumoral , Chlorocebus aethiops , Ensayos de Selección de Medicamentos Antitumorales , Fermentación , Humanos , Espectroscopía de Resonancia Magnética , Estructura Molecular , Plasmodium falciparum/efectos de los fármacos , Espectrometría de Masa por Ionización de Electrospray , Relación Estructura-Actividad , Células VeroRESUMEN
Sixteen previously undescribed lanostane-type triterpenoids (1-16), together with fourteen known compounds, were isolated from cultivated fruiting bodies of the basidiomycete Ganoderma casuarinicola, a recently described species. The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry data. Two of these compounds, 9 and 10, showed antimalarial activity with IC50 values of 9.7 and 9.2 µg/ml, respectively.
Asunto(s)
Antimaláricos/farmacología , Antituberculosos/farmacología , Ganoderma/química , Lanosterol/farmacología , Malaria/tratamiento farmacológico , Fitoquímicos/farmacología , Triterpenos/farmacología , Animales , Antimaláricos/química , Antimaláricos/metabolismo , Antituberculosos/química , Antituberculosos/metabolismo , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Relación Dosis-Respuesta a Droga , Cuerpos Fructíferos de los Hongos/química , Cuerpos Fructíferos de los Hongos/metabolismo , Ganoderma/metabolismo , Lanosterol/análogos & derivados , Lanosterol/química , Lanosterol/metabolismo , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Mycobacterium tuberculosis/efectos de los fármacos , Fitoquímicos/química , Fitoquímicos/metabolismo , Triterpenos/química , Triterpenos/metabolismo , Células Vero , Madera/química , Madera/metabolismoRESUMEN
Four xanthones (1â4) and a known compound, mansonone D (5), were isolated from the lignicolous freshwater fungus BCC 28210 (family, Chaetosphaeriaceae). The structures of these compounds were elucidated by extensive spectroscopic analysis. Among the isolated metabolites, compound 2 and the known mansonone D (5) displayed antimalarial activity against Plasmodium falciparum K1 with IC50 values of 7.75 and 0.55 µg/mL, respectively. Compound 4 displayed antibacterial activity against Bacillus cereus with an MIC value of 6.25 µg/mL.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antimaláricos/aislamiento & purificación , Ascomicetos/química , Xantonas/aislamiento & purificación , Antibacterianos/química , Antimaláricos/química , Ascomicetos/metabolismo , Bacillus cereus/efectos de los fármacos , Agua Dulce/microbiología , Concentración 50 Inhibidora , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Naftoquinonas/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Plasmodium falciparum/efectos de los fármacos , Xantonas/química , Xantonas/farmacologíaRESUMEN
Thirty-one highly modified lanostanes (1-31), together with 19 known compounds (32-50), were isolated from fruiting bodies of the wood-rot basidiomycete Tomophagus sp. The structures were elucidated by analyses of HRMS and NMR spectroscopic data. The present work demonstrates the high structural diversity of modified lanostane triterpenoids from Tomophagus. This paper also discusses structural revisions of several known derivatives. Some of the isolated compounds exhibited moderate antimalarial activity against Plasmodium falciparum K1 (IC50 5.1-19 µM).
Asunto(s)
Basidiomycota/química , Cuerpos Fructíferos de los Hongos/química , Triterpenos/aislamiento & purificación , Lanosterol/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Plasmodium falciparum/efectos de los fármacos , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
A new lanostane triterpene (1), together with three known compounds (2-4), were isolated from cultivated fruiting bodies of the basidiomycete Ganoderma australe. The structure was elucidated on the basis of NMR spectroscopic and mass spectrometry data. The olefinic geometry of methyl australate (2) was revised from 20(22)Z to 20(22)E. These compounds (1-4) were different from the lanostanes isolated from mycelial cultures of the same strain source.
Asunto(s)
Antibacterianos/farmacología , Cuerpos Fructíferos de los Hongos/química , Ganoderma/química , Triterpenos/química , Antibacterianos/química , Evaluación Preclínica de Medicamentos/métodos , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Espectrometría de Masa por Ionización de Electrospray , Triterpenos/farmacologíaRESUMEN
An endophytic actinomycete strain BCC72023 was isolated from rice (Oryza sativa L.) and identified as the genus Streptomyces, based on phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses. The strain showed 99.80% similarity compared with Streptomyces samsunensis M1463(T). Chemical investigation led to the isolation of three macrolides, efomycins M (1), G (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-O-methylabierixin (5). To our knowledge, this is the first report of efomycin M being isolated from a natural source. The compounds were identified using spectroscopic techniques and comparison with previously published data. All compounds exhibited antimalarial activity against the Plasmodium falciparum, K-1 strain, a multidrug-resistant strain, with IC50 values in a range of 1.40-5.23 µg/ml. In addition, these compounds were evaluated for biological activity against Mycobacterium tuberculosis, Bacillus cereus, Colletotrichum gloeosporioides and Colletotrichum capsici, as well as cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells.
Asunto(s)
Antiinfecciosos/análisis , Productos Biológicos/análisis , Endófitos/química , Oryza/microbiología , Streptomyces/química , Antineoplásicos/análisis , Técnicas de Tipificación Bacteriana , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Análisis Espectral , Streptomyces/clasificación , Streptomyces/genética , Streptomyces/aislamiento & purificaciónRESUMEN
Three new angucyclinones, saccharosporones A, B and C, together with (+)-ochromycinone, (+)-rubiginone B2, tetrangulol methyl ether and fujianmycin A, were obtained from fermentation of the terrestrial actinomycete of the genus Saccharopolyspora BCC 21906 isolated from a soil collected in Chanthaburi Province, Thailand. Structures of the new compounds and their relative configurations were assigned by NMR spectral data interpretation. Saccharosporones A and B exhibited antimalarial activity against Plasmodium falciparum K1 with IC50 values of 4.1 and 3.9 µM. Both metabolites also possessed cytotoxic activities against cancer cell lines (KB, MCF-7 and NCI-H187) and nonmalignant Vero cell, while saccharosporone C only showed cytotoxic activity against NCI-H187.
Asunto(s)
Antraquinonas/aislamiento & purificación , Antibióticos Antineoplásicos/aislamiento & purificación , Antimaláricos/aislamiento & purificación , Saccharopolyspora/química , Microbiología del Suelo , Animales , Antraquinonas/química , Antraquinonas/farmacología , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Antibióticos Antineoplásicos/química , Antibióticos Antineoplásicos/farmacología , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Antimaláricos/química , Antimaláricos/farmacología , Bacillus cereus/efectos de los fármacos , Benzo(a)Antracenos/aislamiento & purificación , Benzo(a)Antracenos/farmacología , Candida albicans/efectos de los fármacos , Chlorocebus aethiops , Fermentación , Humanos , Concentración 50 Inhibidora , Células KB , Células MCF-7 , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Mycobacterium tuberculosis/efectos de los fármacos , Plasmodium falciparum/efectos de los fármacos , Tailandia , Células VeroRESUMEN
Sterostreins A-E (1, 2, 3a/3b, 4, and 5), five novel terpenoids, were isolated from cultures of the mushroom fungus Stereum ostrea BCC 22955. Sterostrein A (1) exhibited antimalarial activity (IC(50) 2.3 µg/mL) and cytotoxicity (IC(50) 5.3-38 µg/mL).
Asunto(s)
Antimaláricos/farmacología , Hongos/metabolismo , Mycobacterium tuberculosis/efectos de los fármacos , Plasmodium falciparum/efectos de los fármacos , Terpenos/farmacología , Antimaláricos/química , Antimaláricos/aislamiento & purificación , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Hongos/química , Humanos , Células KB , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Estereoisomerismo , Relación Estructura-Actividad , Terpenos/química , Terpenos/aislamiento & purificaciónRESUMEN
Two strobilurins, 9-methoxystrobilurin B (1) and 9-methoxystrobilurin G (2), two monochlorinated 2,3-dihydro-1-benzoxepin derivatives, 3 and 4a, and butenolide 5, together with four known compounds, strobilurin B, 9-methoxystrobilurin A, and oudemansins A and B, were isolated from culture BCC 18689 of the fungus Favolaschia tonkinensis. 9-Methoxystrobilurins A, B (1), and G (2) and oudemansins A and B exhibited antimalarial, antifungal, and cytotoxic activities, while compounds 3, 4a, and 5 displayed only cytotoxic activity.