[Autoimmune markers for non-invasive diagnosis of endometriosis in women]. / Autoimmunnye markery dlia neinvazivnoi diagnostiki éndometrioza u zhenshchin.

Endometriosis is a common estrogen-dependent chronic disease in women of reproductive age; it is associated with dysregulation of the immune response, local inflammation, and increased formation of autoantibodies. The aim of the study was to investigate the profile of autoantibodies in women with endometriosis and to evaluate their diagnostic value using new modifications of enzyme immunoassay. In women with endometriosis of stage III-IV (n=39), a wide spectrum of autoantibodies was detected, mainly of class G, including antibodies to endometrial antigens (tropomyosin 3, tropomodulin 3), the enzyme α-enolase, steroid (estradiol, progesterone) and gonadotropic hormones. At the same time, the frequency of detection of IgG antibodies to tropomyosin 3, α-enolase, estradiol and human chorionic gonadotropin and their levels in patients with endometriosis were higher than in healthy women (n=26) (p<0.05). IgG-antibodies to tropomyosin 3, α-enolase and estradiol were characterized by higher diagnostic value for endometriosis. The diagnostic value was significantly increased when these antibodies were combined: the AUC reached 0.875 [0.772-0.978] (p<0.0001), the sensitivity and specificity were 83.3% each. Thus, autoantibodies to tropomyosin 3, α-enolase, and estradiol are promising for inclusion in the panel of biomarkers for non-invasive diagnosis of endometriosis.

[Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell cultures isolated from human endometrium and umbilical cord]. / Sravnenie profilia ékspressii poverkhnostnykh molekuliarnykh markerov na mezenkhimnykh stromal'nykh kletkakh kul'tur, poluchennykh iz éndometriia i pupoviny cheloveka.

Endometrial mesenchymal stromal cells (eMSCs), along with mesenchymal stromal cells (MSCs) isolated from other tissues, are promising for use in regenerative medicine. The benefits of eMSCs include their presence in adults, simplicity of isolation, high proliferative and differentiation capacity. In this study, we have employed the flow cytometry technique to assess expression of 28 molecular markers on the surface of two eMSCs cultures. The culture of MSCs isolated from Wharton's jelly of the umbilical cord (uMSCs) was used as a reference, because uMSCs were studied in details earlier and demonstrated their effectiveness in vivo. Both types of MSCs demonstrated similar expression profiles. They included stem cells surface molecules, cell adhesion molecules and their ligands, some receptor molecules responsible for cell metabolism and proliferation, as well as immunological response molecules.

Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium and Endometriosis Lesions.

Cell cultures isolated from endometriosis lesions by enzymatic dissociation consisted of fibroblast-like cells expressing CD90, CD73, and CD105; cell viability in these cultures was >90%, but this parameter decreased by passage 3. Zero passage cultures contained 10-25% epithelial cells expressing cytokeratin-7, but by passage 2, the cultures became more homogeneous and epithelial cells disappeared. The proportion of proliferating cells and population doubling level increased from passage 1 to passage 3. The cultures from the endometrium were induced to adipogenic and osteogenic differentiation in vitro. The cultures derived from ectopic endometrium have properties of multipotent mesenchymal stromal cells that exhibited in vitro similarities and differences from cell cultures from eutopic endometrium, which allows using this cell model for the search and testing of new drugs and technologies aimed at suppression of the growth and spread of endometriosis lesions.

Comparative Analysis of the Proliferative Potential of Human Mesenchymal Stromal Cells from Extraembryonic Organs, Endometrium, and Adipose Tissue.

Proliferative activity of mesenchymal stromal cells isolated from five sources (chorionic villi, Wharton's jelly, amnion, endometrium, and adipose tissue) was compared by flow cytometry and real-time PCR (by the content of mRNA of genes encoding of cell cycle regulators). Mesenchymal stromal cells derived from the endometrium demonstrated maximum stability and high proliferative potential.

Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium.

Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7(+) epithelial cells that disappeared by passage 2. The cultures from the endometrium could be induced to adipogenic, osteogenic and chondrogenic differentiation in vitro. These findings suggest that human endometrium is a convenient source of biomaterial for minimally invasive isolation of cultures that exhibit typical morphology and immunophenotypic profile of resident multipotent mesenchymal stromal cells retain high viability in vitro.

[Iliac lipoleiomyoma with development of invagination and acute ileus].

Lipoleiomyoma is a benign tumor that consists of smooth muscle and fat components, which occurs in less than 0.5% of cases among all benign intestine tumors. The paper describes a case of acute ileum obstruction with the development of lipoleiomyoma invagination in a 23-year-old woman and presents the endoscopic verification and microscopic and immunohistochemical examination of the removed tumor.

[Endoscopic diagnostics of colorectal endometriosis].

UNLABELLED: The purpose of the study is to explore and systematize the endoscopic features of colorectal endometriosis and to evaluate the role of colonoscopy in its diagnosis. MATERIAL AND METHODS: Were analyzed 320 diagnostic colonoscopies performed during the period from March 2011 till November 2012 in patients admitted to the hospital with a diagnosis of "infiltrative form of external endometriosis". RESULTS: Among all the identified cases of intestinal endometriosis (n = 67) pathological process was localized more often in the upper section of the rectum ampullar - 38 (56.7%), less often were revealed lesions of lower ampullar section of rectum and anal canal in 5 (7.4%). In 6 (8.9%) cases were detected multifocal lesions of the intestine. Authentic signs of colorectal endometriosis were detected in lesions of the mucous membrane. These include: polypoid growths above the endometriosis lesion (15 (22.4%)), endometrioid heterotopias in the colon mucosa (7 (10.4%)), the presence of ulceration of the mucous membrane in the projection of endometriosis (4 (5.9%)) geoidal mucosa (3 (4.5%)). Colorectal endometriosis can be systematized according to the following endoscopic criteria: localization of the pathological focus, growth, the presence of mucosal lesions.