Predictors of cardiopulmonary exercise testing in COPD patients according to the Weber classification.

BACKGROUND: Weber classification stratifies cardiac patients based on peak oxygen consumption (V̇O2), the gold-standard measure of exercise capacity. OBJECTIVE: To determine if Weber classification is a useful tool to discriminate clinical phenotypes in COPD patients and to evaluate if disease severity and other clinical measures can predict V̇O2peak. METHODS: Three hundred and six COPD patients underwent cardiopulmonary exercise testing (CPX) and were divided according to Weber class: 1) Weber A (n = 34); 2) Weber B (n = 88); 3) Weber C (n = 138); and 4) Weber D (n = 46). RESULTS: Weber class D patients demonstrated a reduced V̇O2 peak, heart rate (HR), minute ventilation (V̇E), oxygen (O2) pulse, circulatory power (CP), oxygen uptake efficiency slope (OUES), oxygen saturation (SpO2%), delta (Δ)HR and ΔSpO2 when compared to Weber A and B (p<0.05). Moreover, Dyspnea and the V̇E/carbon dioxide production (V̇CO2) slope were higher in Weber D compared with Weber C and A (p<0.001). Hierarchical regression analysis demonstrated significant predictors of V̇O2peak (R2= 0.131; Adj R 2 = 1.25), including HR (ß=0.5757; t = 5.7; P<0.001) and forced expiratory volume in one second (FEV1) (ß=0.119; t = 2.16; P<0.03). Among the Weber C + D groups, predictors of V̇O2peak (R = 0.78; R2= 0.60; Adj R2 =0.59), dyspnea (ß=0.076; t = 1.111; P<0.27) and maximal voluntary ventilation (MVV) (ß=0.75; t = 1.14; P<0.00). CONCLUSION: Weber classification may be a useful tool to stratify cardiorespiratory fitness in COPD patients. Other clinical measures may be useful in predicting peak V̇O2 in mild-to-severe COPD, moreover different phenotypes may be important tool to improve physical capacity of chronic disease patients.

Fabrication of antibacterial and biocompatible 3D printed Manuka-Gelatin based patch for wound healing applications.

Development of multifunctional 3D patches with appropriate antibacterial and biocompatible properties is needed to deal with wound care regeneration. Combining gelatin-based hydrogel with a well-known natural antibacterial honey (Manuka honey, MH) in a 3D patch can provide improved printability and at the same time provide favourable biological effects that may be useful in regenerative wound treatment. In this study, an antibacterial Manuka-Gelatin 3D patches was developed by an extrusion-based printing process, with controlled porosity, high shape fidelity, and structural stability. It was demonstrated the antibacterial activity of Manuka-Gelatin 3D patches against both gram-positive bacteria (S. epidermidis and S. aureus) and gram-negative (E. coli), common in wound infection. The 3D Manuka-Gelatin base patches demonstrated antibacterial activity, and moreover enhanced the proliferation of human dermal fibroblasts and human epidermal keratinocytes, and promotion of angiogenesis. Moreover, the ease of printing achieved by the addition of honey, coupled with the interesting biological response obtained, makes this 3D patch a good candidate for wound healing applications.

Physical Training Reduces Chronic Airway Inflammation and Mediators of Remodeling in Asthma.

Several benefits of aerobic training for asthmatic patients have been demonstrated. However, its effects on systemic inflammation and on airway remodeling mediators and lung mechanics are unknown. This prospective study included 21 intermittent and mild asthma patients, and as primary outcomes, the evaluation of pro- and anti-inflammatory and pro- and antifibrotic mediators in exhaled breath condensate (EBC) and blood were performed, beyond the cell counting in blood and in induced sputum. Aerobic training was performed for 3 months, 3 times per week. Aerobic training increased the levels of anti-inflammatory cytokines and of antifibrotic mediators in the breath condensate: IL-1ra (p = 0.0488), IL-10 (p = 0.0048), relaxin-3 (p = 0.0019), and klotho (p < 0.0043), respectively. Similarly, in plasma, increased levels of IL-1ra (p = 0.0147), IL-10 (p < 0.0001), relaxin-3 (p = 0.004), and klotho (p = 0.0023) were found. On contrary, reduced levels of proinflammatory cytokines in the breath condensate, IL-1ß (p = 0.0008), IL-4 (p = 0.0481), IL-5 (p < 0.0001), IL-6 (p = 0.0032), IL-13 (p = 0.0013), and TNF-α (p = 0.0001) and profibrotic markers VEGF (p = 0.0017) and TSLP (p = 0.0056) were found. Similarly, in plasma, aerobic training significantly reduced the levels of proinflammatory cytokines IL-1ß (p = 0.0008), IL-4 (p = 0.0104), IL-5 (p = 0.0001), IL-6 (p = 0.006), IL-13 (p = 0.0341), and TNF-α (p = 0.0003) and of profibrotic markers VEGF (p = 0.0009) and TSLP (p < 0.0076). Fractional exhaled nitric oxide (FeNO) was reduced after the intervention (p = 0.0313). Regarding inflammatory cells in sputum, there was a reduction in total cells (p = 0.008), eosinophils (p = 0.009), and macrophages (p = 0.020), as well as of blood eosinophils (p = 0.0203) and lymphocytes (p = 0.0198). Aerobic training positively modulates chronic airway inflammation and remodeling mediators, beyond to improve systemic inflammation in intermittent and mild asthmatic patients.

Combined resistance and aerobic training improves lung function and mechanics and fibrotic biomarkers in overweight and obese women.

Background: Obesity impairs lung function and mechanics and leads to low-grade inflammation, but the effects of combined physical exercise (CPE) on that are unknown. Methods: We investigated the effects of 12 weeks of combined physical exercise (aerobic + resistance training), in non-obese (n = 12), overweight (n = 17), and obese grade I (n = 11) women. Lung function and lung mechanics were evaluated. The systemic immune response was evaluated by whole blood analysis and biomarker measurements, while pulmonary fibrotic biomarkers were evaluated in the breath condensate. Result: CPE improved forced vital capacity (FVC) % (p < 0.001) and peak expiratory flow (PEF) % (p < 0.0003) in the obese group; resistance of the respiratory system (R5Hz) in non-obese (p < 0.0099), overweight (p < 0.0005), and obese (p < 0.0001) groups; resistance of proximal airways (R20Hz) in non-obese (p < 0.01), overweight (p < 0.0009), and obese (p < 0.0001) groups; resistance of distal airways (R5Hz-R20Hz) in non-obese (p < 0.01), overweight (p < 0.0012), and obese (p < 0.0001) groups; reactance of the respiratory system (X5Hz) in non-obese (p < 0.01), overweight (p < 0.0006), and obese (p < 0.0005) groups; impedance of the respiratory system (Z5Hz) in non-obese (p < 0.0099), overweight (p < 0.0005), and obese (p < 0.0001) groups; central resistance (RCentral) in non-obese (p < 0.01), overweight (p < 0.001), and obese (p < 0.0003) groups; and the peripheral resistance (RPeripheral) in non-obese (p < 0.03), overweight (p < 0.001), and obese (p < 0.0002) groups. CPE reduced the pro-fibrotic IGF-1 levels in BC in overweight (p < 0.0094) and obese groups (p < 0.0001) and increased anti-fibrotic Klotho levels in BC in obese (p < 0.0001) groups, and reduced levels of exhaled nitric oxide in overweight (p < 0.03) and obese (p < 0.0001) groups. Conclusion: CPE improves lung function, mechanics, and pulmonary immune response in overweight and obese grade I women by increasing anti-fibrotic protein Klotho and reducing pro-fibrotic IGF-1.

Responses of different functional tests in candidates for bariatric surgery and the association with body composition, metabolic and lipid profile.

Individuals with obesity can have metabolic disorders and may develop impairments that affect the ability to exercise. The maximal incremental cardiopulmonary exercise test is widely used to assess functional capacity. However, submaximal tests such as the two-minute step test (2MST) and the six-minute walk test (6MWT) also allow this assessment. We propose to analyze whether body composition, metabolic and lipid profile influence the maximal and submaximal performance, and investigate these variables in response to different functional tests. Forty-four individuals with obesity, aged 18-50 years, underwent analysis of body composition, metabolic and lipid profile, incremental treadmill test (ITMT), 6MWT, and 2MST. One-way ANOVA, Pearson or Spearman correlation, and Stepwise multiple linear regression analysis were performed. ITMT induced a greater metabolic, ventilatory, cardiovascular, and perceived exertion demand when compared to the 6MWT and 2MST (p < 0.05). In addition, 2MST elicited a higher chronotropic (HR) and metabolic (V̇O2) demand when compared to the 6MWT (p < 0.05). Significant correlations were found between tests and body composition, metabolic and lipid profile. Fat mass and low-density lipoprotein can explain 30% of the V̇O2 variance in the ITMT; and fat mass, glucose, and performance in the 2MST can explain 42% of the variance of the distance walked in the ITMT. Obesity and its metabolic impairments are capable of influencing responses to exercise. ITMT generated greater demand due to the high stress imposed, however, 2MST demanded greater metabolic and chronotropic demand when compared to the 6MWT.

Pulmonary function changes in older adults with and without metabolic syndrome.

The low-grade inflammation associated with metabolic syndrome (MS) triggers functional and structural alterations in several organs. Whereas lung function impairment is well reported for older adult population, the effect of MS on functional and immunological responses in the lungs remains unclear. In this cross-sectional study we determined whether MS alters pulmonary function, and immunological responses in older adults with MS. The study sample consisted of older adults with MS (68 ± 3 years old; n = 77) and without MS (67 ± 3 years old; n = 77). Impulse oscillometry was used to evaluate airway and tissue resistance, and reactance. Biomarkers of inflammation and fibrosis were assessed in the blood and in breath condensate. The total resistance of the respiratory system (R5Hz; p < 0.009), and the resistance of the proximal (R20Hz; p < 0.001) and distal (R5Hz-R20Hz; p < 0.004) airways were higher in MS individuals compared to those without MS. Pro-inflammatory (leptin, IL-1beta, IL-8, p < 0.001; TNF-alpha, p < 0.04) and anti-inflammatory cytokines (adiponectin, IL-1ra, IL-10, p < 0.001), anti-fibrotic (relaxin 1, relaxin 3, Klotho, p < 0.001) and pro-fibrotic (VEGF, p < 0.001) factors were increased in sera and in breath condensate individuals with MS. The results show that MS adversely affect lung mechanics, function, and immunological response in older adults. The data offer a metabolic basis for the inflammaging of the lungs and suggest the lungs as a potential therapeutic target for controlling the immune response and delaying the onset of impaired lung function in older adults with MS.

Evolutionary pluralism and the ideal of a unified biology. / Pluralismo evolutivo e o ideal de unificação da biologia.

Biological evolution is often regarded as a central and unifying axis of biology. This article discusses historical aspects of this ideal of unification, as well as signs of its disintegration from the 1960s to 1980s. We argue that despite new proposals for the synthesis of biological knowledge, contemporary evolutionary biology is characterized by pluralism. The main points in favor of evolutionary pluralism are discussed and some consequences of this perspective are presented, particularly in terms of the ideal of a unified biology. Finally, we defend an evolutionary pluralism that critiques the ideal of unification as a scientific objective, but still favors local integrations.

A evolução biológica é frequentemente considerada um eixo central e unificador da biologia. O artigo discute aspectos históricos desse ideal de unificação, bem como os seus sinais de desintegração entre os anos 1960 e 1980. Argumentamos que apesar das novas propostas de síntese do conhecimento biológico, a biologia evolutiva contemporânea é caracterizada por um pluralismo. Os principais pontos a favor do pluralismo evolutivo são discutidos, e algumas consequências dessa perspectiva são apresentadas, particularmente em relação ao ideal de unificação da biologia. Por fim, defendemos um pluralismo evolutivo crítico do ideal de unificação como um objetivo da ciência, mas ainda favorável a integrações locais.

Pluralismo evolutivo e o ideal de unificação da biologia / Evolutionary pluralism and the ideal of a unified biology

Resumo A evolução biológica é frequentemente considerada um eixo central e unificador da biologia. O artigo discute aspectos históricos desse ideal de unificação, bem como os seus sinais de desintegração entre os anos 1960 e 1980. Argumentamos que apesar das novas propostas de síntese do conhecimento biológico, a biologia evolutiva contemporânea é caracterizada por um pluralismo. Os principais pontos a favor do pluralismo evolutivo são discutidos, e algumas consequências dessa perspectiva são apresentadas, particularmente em relação ao ideal de unificação da biologia. Por fim, defendemos um pluralismo evolutivo crítico do ideal de unificação como um objetivo da ciência, mas ainda favorável a integrações locais.

Abstract Biological evolution is often regarded as a central and unifying axis of biology. This article discusses historical aspects of this ideal of unification, as well as signs of its disintegration from the 1960s to 1980s. We argue that despite new proposals for the synthesis of biological knowledge, contemporary evolutionary biology is characterized by pluralism. The main points in favor of evolutionary pluralism are discussed and some consequences of this perspective are presented, particularly in terms of the ideal of a unified biology. Finally, we defend an evolutionary pluralism that critiques the ideal of unification as a scientific objective, but still favors local integrations.

Whole-body electrical stimulation as a strategy to improve functional capacity and preserver lean mass after bariatric surgery: a randomized triple-blind controlled trial.

BACKGROUND/OBJECTIVES: Bariatric surgery (BS) is a successful, long-lasting treatment option for obese. The early postoperative (PO) period is followed by dietary restriction and physical inactivity, leading to declines in muscle mass and functional capacity. Whole-body electromyostimulation (WB-EMS) may be a feasible and potential early rehabilitation strategy post BS. The aim was to evaluate the effects of WB-EMS with exercise training (Fe) on functional capacity, body composition, blood biomarkers, muscle strength, and endurance post BS. SUBJECTS/METHODS: This is a randomized, triple-blind, sham-controlled trial. Thirty-five volunteers underwent a Roux-en-Y gastric bypass and were randomized into a WB-EMS (WB-EMSG) or control group (ShamG). Preoperative evaluations consisted of maximal and submaximal exercise testing, body composition, blood biomarkers, quadriceps strength, and endurance. After discharge, functional capacity and body composition were obtained. Exercise training protocols in both groups consisted of 14 dynamic exercises, 5 days per week, completing 30 sessions. The WB-EMSG also underwent an electrical stimulation protocol (Endurance: 85 Hz, 350 ms, 6 s of strain, 4 f of rest; Strength: 30 Hz, 350 ms, 4 s of strain, 10 seconds of rest, with bipolar electrical pulse). After intervention, subjects were reevaluated. RESULTS: The protocol started on average 6.7 ± 3.7 days after discharge. Both groups presented with a decline in functional capacity after BS (p < 0.05) and a reduction in all body composition measurements (p < 0.05). The exercise training program led to significant improvements in functional capacity (ShamG - PO: 453.8 ± 66.1 m, Post: 519.2 ± 62.8 m; WB-EMSG- PO: 435.9 ± 74.5, Post: 562.5 ± 66.4 m, p < 0.05), however, only the WB-EMSG demonstrated significant changes of distance walked (interaction time vs group effect, p < 0.05). In addition, adiponectin significantly increased only in the WB-EMSG (p < 0.05). The WB-EMSG was also able to preserve muscle strength, endurance, and fatigue index, while the ShamG demonstrated significant decline (p < 0.05). CONCLUSION: WB-EMS + Fe can be an attractive and feasible method following BS to enhance functional capacity and prevent deterioration of muscle function in the early PO. CLINICAL TRIAL REGISTRATION: ReBEC, RBR-99qw5h, on 20 February 2015.

3D-printed platform multi-loaded with bioactive, magnetic nanoparticles and an antibiotic for re-growing bone tissue.

Polymeric platforms obtained by three-dimensional (3D) printing are becoming increasingly important as multifunctional therapeutic systems for bone treatment applications. In particularly, researchers aim to control bacterial biofilm on these 3D-platforms and enhance re-growing bone tissue, at the same time. This study aimed to fabricate a 3D-printed polylactic acid platform loaded with hydroxyapatite (HA), iron oxide nanoparticles (IONPs) and an antibiotic (minocycline) with tuneable properties and multistimuli response. IONPs were produced by a facile chemical co-precipitation method showing an average diameter between 11 and 15 nm and a superparamagnetic behaviour which was preserved when loaded into the 3D-platforms. The presence of two types of nanoparticles (IONPs and HA) modify the nanomorphological/nanotopographical feature of the 3D-platforms justifying their adequate bioactivity profile and in vitro cellular effects on immortalized and primary osteoblasts, including cytocompatibility and increased osteogenesis-related gene expression (RUNX2, BGLAP and SPP1). Disk diffusion assays and SEM analysis confirmed the effect of the 3D-platforms loaded with minocycline against Staphylococcus aureus. Altogether results showed that fabricated 3D-platforms combined the exact therapeutic antibiofilm dose of the antibiotic against S. aureus, with the enhanced osteogenic stimulation of the HA and IONPs nanoparticles which is a disruptive approach for bone targeting applications.

Effects of Starch Incorporation on the Physicochemical Properties and Release Kinetics of Alginate-Based 3D Hydrogel Patches for Topical Delivery.

The development of printable hydrogel inks for extrusion-based 3D printing is opening new possibilities to the production of new and/or improved pharmaceutical forms, specifically for topical application. Alginate and starch are natural polysaccharides that have been extensively exploited due to their biocompatibility, biodegradability, viscosity properties, low toxicity, and relatively low cost. This research work aimed to study the physicochemical and release kinetic effects of starch incorporation in alginate-based 3D hydrogel patches for topical delivery using a quality by design approach. The incorporation of a pregelatinized starch is also proposed as a way to improve the properties of the drug delivery system while maintaining the desired quality characteristics. Critical material attributes and process parameters were identified, and the sensitivity and adequacy of each parameter were statistically analyzed. The impact of alginate, starch, and CaCl2·2H2O amounts on relevant quality attributes was estimated crosswise. The amount of starch revealed a synergetic impact on porosity (p = 0.0021). An evident increase in the size and quantity of open pores were detected in the as printed patches as well as after crosslinking (15.6 ± 5.2 µm). In vitro drug release studies from the optimized alginate-starch 3D hydrogel patch, using the probe Rhodamine B, showed an initial high burst release, followed by a controlled release mechanism. The results obtained also showed that the viscoelastic properties, printing accuracy, gelation time, microstructure, and release rates can be modulated by varying the amount of starch added to the system. Furthermore, these results can be considered an excellent baseline for future drug release modulation strategies.

Effects of acute inspiratory loading during treadmill running on cerebral, locomotor and respiratory muscle oxygenation in women soccer players.

Respiratory limitation can be a primary mechanism for exercise cessation in female athletes. This study aimed to assess the effects of inspiratory loading (IL) on intercostal muscles (IM), vastus lateralis (VL) and cerebral (Cox) muscles oxygenation in women soccer players during high-intensity dynamic exercise. Ten female soccer players were randomized to perform in order two constant-load tests on a treadmill until the exhaustion time (Tlim) (100 % of maximal oxygen uptake- V˙O2). They breathed freely or against a fixed inspiratory loading (IL) of 41 cm H2O (∼30 % of maximal inspiratory pressure). Oxygenated (Δ[OxyHb]), deoxygenated (Δ[DeoxyHb]), total hemoglobin (Δ[tHb]) and tissue saturation index (ΔTSI) were obtained by NIRs. Also, blood lactate [La-] was obtained. IL significantly reduced Tlim (224 ± 54 vs 78 ± 20; P < 0.05) and increased [La-], V˙O2, respiratory cycles and dyspnea when corrected to Tlim (P < 0.05). IL also resulted in decrease of Δ[OxyHb] of Cox and IM during exercise compared with rest condition. In addition, decrease of Δ[OxyHb] was observed on IM during exercise when contrasted with Sham (P < 0.05). Furthermore, significant higher Δ[DeoxyHb] of IM and significant lower Δ[DeoxyHb] of Cox were observed when IL was applied during exercise in contrast with Sham (P < 0.05). These results were accompanied with significant reduction of Δ[tHb] and ΔTSI of IM and VL when IL was applied (P < 0.05). High-intensity exercise with IL decreased respiratory and peripheral muscle oxygenation with negative impact on exercise performance. However, the increase in ventilatory work did not impact cerebral oxygenation in soccer players.

Overlap syndrome: the coexistence of OSA further impairs cardiorespiratory fitness in COPD.

BACKGROUND: Cardiorespiratory fitness (CRF) is an important prognostic marker in chronic obstructive pulmonary disease (COPD). Obstructive sleep apnea (OSA) also negatively affects exercise tolerance. However, the impact of their association on CRF has not been evaluated. We hypothesized that patients with overlap syndrome would demonstrate a greater impairment in CRF, particularly those with severe COPD. METHODS: Individuals with COPD were recruited. First, subjects underwent clinical and spirometry evaluation. Next, home-based sleep evaluation was performed. Subjects with an apnea-hypopnea index (AHI) < 15 episodes/h were allocated to the COPD group and those with an AHI ≥ 15 episodes/h to the overlap group. On the second visit, subjects underwent a cardiopulmonary exercise test. Subsequently, they were divided into four groups according to the severity of COPD and coexistence of OSA: COPDI/II; overlap I/II; COPDIII/IV; and overlap III/IV. RESULTS: Of the 268 subjects screened, 31 were included. The overlap group exhibited higher values for peak carbon dioxide (COPD: 830 [678-1157]; overlap: 1127 [938-1305] mm Hg; p < 0.05), minute ventilation (COPD: 31 [27-45]; overlap: 48 [37-55] L; p < 0.05), and peak systolic blood pressure (COPD: 180 [169-191]; overlap: 220 [203-227] mm Hg; p <; 0.001) and peak diastolic blood pressure COPD: 100 [93-103]; overlap: 110 [96-106] mm Hg; p < 0.001). COPD severity associated with OSA produced a negative impact on exercise time (COPDIII/IV: 487 ± 102; overlap III/IV: 421 ± 94 s), peak oxygen uptake (COPDIII/IV: 12 ± 2; overlap III/IV: 9 ± 1 ml.Kg.min-1 ; p < 0.05) and circulatory power (COPDIII/IV: 2306 ± 439; overlap III/IV: 2162 ± 340 ml/kg/min.mmHg; p < 0.05). CONCLUSION: Overlap syndrome causes greater hemodynamic and ventilatory demand at the peak of dynamic exercise. In addition, OSA overlap in individuals with more severe COPD impairs CRF.

Accuracy of air temperature forecasts and its use for prediction of the reference evapotranspiration / Acurácia das previsões da temperatura do ar e uso na estimativa da evapotranspiração de referência

The objective of this study was to verify the quality of the short-term forecasts made by some web sites, as well as the potential of its use in estimating reference evapotranspiration (ETo). For the period 2012-2013, every 4-days, the maximum (Tmax) and minimum (Tmin) air temperature forecasts for horizons of 24, 48, 72 and 96 hours provided by public sites for Uberlandia-MG were collected and used for calculating the reference evapotranspiration (ETo) by the Hargreaves-Samani (HS) calibrated equation. The sites www.accuweather.com (WEATHER); www.cemig.com.br (CEMIG); jornaldotempo.uol.com.br; (JORNAL) and www.climatempo.com.br (CLIMA) were evaluated. For the same period, the meteorological data were taken at the National Institute of Meteorology (www.inmet.gov.br). The performance of the Tmax and Tmin forecasts as well as the ETo estimates were made by regression analysis, the mean bias error (MBE), root mean square error (RMSE) and t-test. For all sites, there was a better performance in the prediction of Tmin when compared to Tmax, especially by a reduction in errors (MBE and RMSE) and an increase in coefficients b and R2. For ETo, the site CLIMA had lowest MBE and RMSE (0.9 mm d-1) following by the WEATHER and JORNAL. On the other hand, the site CEMIG presented higher values for MBE (-1.3 mm d-1) and RMSE (1.6 mm d-1). There was no worsening with an increase of the forecast horizon in these indices, except for R² values. The site CLIMA was the only one that did not differ significantly from the ETo values observed. The results of diagnostic verification procedures indicate the potential use of air temperature forecasts in irrigation management.

O objetivo deste trabalho foi verificar a qualidade das previsões de curto prazo feitas por alguns endereços eletrônicos da internet, bem como a potencialidade de seu uso na estimativa da evapotranspiração de referência (ETo). No período de 2012 a 2013, a cada quatro dias, foram coletadas as previsões de 24,48,72 e 96 de horizonte, da temperatura máxima (Tmax) e mínima (Tmin) do ar de quatro endereços eletrônicos para a cidade de Uberlândia, MG. Os dados previstos de temperatura, foram utilizados na equação calibrada do modelo de Hargreaves-Samani (HS) para a obtenção da estimativa da ETo. Os endereços avaliados foram: www.accuweather.com (WEATHER); www.cemig.com.br (CEMIG); jornaldotempo.uol.com.br; (JORNAL) e www.climatempo.com.br (CLIMA). No mesmo período, dados meteorológicos da cidade foram obtidos junto ao Instituto Nacional de Meteorologia (www.inmet.gov.br). A performance das previsões da temperatura e estimativas de ETo foram feitas por meio da análise de regressão, erro médio (EM), raiz quadrada do quadrado médio do erro (RQME) e pelo teste-t. Para todos os endereços, houve uma melhor performance na previsão da Tmin em relação a Tmax, especialmente pela redução dos erros (ME e RQME) e aumento nos coeficientes b e R2. Para ETo, o endereço CLIMA obteve os menores valores de EM e RQME (0,9 mm d-1) seguido do endereço WEATHER e JORNAL. Por outro lado, o endereço CEMIG apresentou os maiores valores para EM (-1,3 mm d-1) e RQME (1,6 mm d-1). Não houve piora na estimativa com aumento do horizonte de previsão nesses índices, exceto para R2. O endereço CLIMA foi o único que não diferiu significativamente dos valores de ETo observados. Os resultados de verificação indicam potencial uso das previsões para o manejo da irrigação.

Engineering a multifunctional 3D-printed PLA-collagen-minocycline-nanoHydroxyapatite scaffold with combined antimicrobial and osteogenic effects for bone regeneration.

3D-printing and additive manufacturing can be powerful techniques to design customized structures and produce synthetic bone grafts with multifunctional effects suitable for bone repair. In our work we aimed the development of novel multifunctionalized 3D printed poly(lactic acid) (PLA) scaffolds with bioinspired surface coatings able to reduce bacterial biofilm formation while favoring human bone marrow-derived mesenchymal stem cells (hMSCs) activity. For that purpose, 3D printing was used to prepare PLA scaffolds that were further multifunctionalized with collagen (Col), minocycline (MH) and bioinspired citrate- hydroxyapatite nanoparticles (cHA). PLA-Col-MH-cHA scaffolds provide a closer structural support approximation to native bone architecture with uniform macroporous, adequate wettability and an excellent compressive strength. The addition of MH resulted in an adequate antibiotic release profile that by being compatible with local drug delivery therapy was translated into antibacterial activities against Staphylococcus aureus, a main pathogen associated to bone-related infections. Subsequently, the hMSCs response to these scaffolds revealed that the incorporation of cHA significantly stimulated the adhesion, proliferation and osteogenesis-related gene expression (RUNX2, OCN and OPN) of hMSCs. Furthermore, the association of a bioinspired material (cHA) with the antibiotic MH resulted in a combined effect of an enhanced osteogenic activity. These findings, together with the antibiofilm activity depicted strengthen the appropriateness of this 3D-printed PLA-Col-MH-cHA scaffold for future use in bone repair. By targeting bone repair while mitigating the typical infections associated to bone implants, our 3D scaffolds deliver an integrated strategy with the combined effects further envisaging an increase in the success rate of bone-implanted devices.

COPD assessment test and FEV1: do they predict oxygen uptake in COPD?

BACKGROUND: Chronic obstructive pulmonary disease (COPD) manifests itself in complex ways, with local and systemic effects; because of this, a multifactorial approach is needed for disease evaluation, in order to understand its severity and impact on each individual. Thus, our objective was to study the correlation between easily accessible variables, usually available in clinical practice, and maximum aerobic capacity, and to determine models for peak oxygen uptake (VO2peak) estimation in COPD patients. SUBJECTS AND METHODS: Individuals with COPD were selected for the study. At the first visit, clinical evaluation was performed. During the second visit, the volunteers were subjected to the cardiopulmonary exercise test. To determine the correlation coefficient of VO2peak with forced expiratory volume in 1 second (FEV1) (% pred.) and the COPD Assessment Test score (CATs), Pearson or Spearman tests were performed. VO2 at the peak of the exercise was estimated from the clinical variables by simple and multiple linear regression analyses. RESULTS: A total of 249 subjects were selected, 27 of whom were included after screening (gender: 21M/5F; age: 65.0±7.3 years; body mass index: 26.6±5.0 kg/m2; FEV1 (% pred.): 56.4±15.7, CAT: 12.4±7.4). Mean VO2 peak was 12.8±3.0 mL⋅kg-1⋅min-1 and VO2peak (% pred.) was 62.1%±14.9%. VO2peak presented a strong positive correlation with FEV1 (% pred.), r: 0.70, and a moderate negative correlation with the CATs, r: -0.54. In the VO2peak estimation model based on the CAT (estimated VO2peak =15.148- [0.185× CATs]), the index explained 20% of the variance, with estimated error of 2.826 mL⋅kg-1⋅min-1. In the VO2peak estimation model based on FEV1 (estimated VO2peak =6.490+ [0.113× FEV1]), the variable explained 50% of the variance, with an estimated error of 2.231 mL⋅kg-1⋅min-1. In the VO2peak estimation model based on CATs and FEV1 (estimated VO2peak =8.441- [0.0999× CAT] + [0.1000× FEV1]), the variables explained 55% of the variance, with an estimated error of 2.156 mL⋅kg-1⋅min-1. CONCLUSION: COPD patients' maximum aerobic capacity has a significant correlation with easily accessible and widely used clinical variables, such as the CATs and FEV1, which can be used to estimate peak VO2.

Effects of inspiratory muscle training in professional women football players: a randomized sham-controlled trial.

This study was conducted to determine the effects of inspiratory muscle training (IMT) on respiratory and peripheral muscles oxygenation during a maximal exercise tolerance test and on repeated-sprint ability (RSA) performance in professional women football players. Eighteen athletes were randomly assigned to one of the following groups: SHAM (n = 8) or IMT (n = 10). After a maximal incremental exercise test, all participants performed (on a different day) a time-to-exhaustion (Tlim) test. Peripheral and respiratory muscles oxygenation by near-infrared spectroscopy, breath-by-breath ventilatory and metabolic variables, and blood lactate concentration were measured. The RSA test was performed on a grass field. After a 6 week intervention, all athletes were reevaluated. Both groups showed increases in inspiratory muscles strength, exercise tolerance and RSA performance, however only the IMT group presented lower deoxyhemoglobin and total hemoglobin blood concentrations on intercostal muscles concomitantly to an increased oxyhemoglobin and total hemoglobin blood concentrations on vastus lateralis muscle during Tlim. In conclusion, these results may indicate the potential role of IMT to attenuate inspiratory muscles metaboreflex and consequently improve oxygen and blood supply to limb muscles during high-intensity exercise, with a potential impact on inspiratory muscle strength, exercise tolerance and sprints performance in professional women football players.

Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults.

Obesity is often associated with metabolic disorders. However, some obese people can present a metabolically healthy phenotype, despite having excessive body fat. Obesity-related cytokines, such as myostatin (MSTN), leptin (LP) and adiponectin (ADP) appear to be key factors for the regulation of muscle and energy metabolism. Our aim was to compare lipid, glucose-insulin and inflammatory (tumor necrosis factor alpha; TNF-α) profiles, muscle function, energy expenditure and aerobic capacity between healthy normal-weight (NW) adults, metabolically healthy obese (MHO) and metabolically unhealthy obese (MUHO) adults; to study the associations between these outcomes and the cytokines MSTN, ADP, LP; and to establish cutoffs for MSTN and LP/ADP to identify the MUHO phenotype. Sixty-one young adults (NW, n = 24; MHO, n = 16; MUHO, n = 21) underwent body composition (body fat -BF and muscle mass - MM), energy expenditure at rest (RER) and aerobic capacity (VO2peak) evaluation, muscle strength and endurance tests and blood profile characterization (glucose-insulin homeostasis and serum MSTN, ADP, LP and TNF-α). MHO and MUHO had a BMI ≥ 30 kg m-2. MUHO was defined as presenting ≥3 criteria for metabolic syndrome (NCEP/ATPIII) in association with insulin resistance (HOMA-IR ≥3.46). MSTN and LP/ADP were associated with MM, MetS and glucose-insulin profile; MSTN was associated with TNF-α and only LP/ADP was associated with parameters of obesity and VO2peak. Neither MSTN nor LP/ADP was associated with muscle functions (p < .05 for adjusted correlations). Both of them were able to discriminate the MUHO phenotype: MSTN [AUC(95%CI) = 0.71(0.55-0.86), MSTN > 517.3 pg/mL] and LP/ADP [AUC(95%CI) = 0.89(0.81-0.97), LP/ADP > 2.14 pg/ng]. In conclusion, high MSTN and LP/ADP are associated with MetS, glucose-insulin homeostasis impairment and low muscle mass. Myostatin is associated with TNF-α and leptin-to-adiponectin ratio is associated with body fatness and aerobic capacity. Neither MSTN nor LP/ADP is associated with energy expenditure, muscle strength and endurance. Myostatin and adipokines cutoffs can identify the metabolically unhealthy obese phenotype in young adults with acceptable accuracy.

Pesticide application windows in the region of Uberlândia-MG, Brazil / Janelas de aplicação de produtos fitossanitários na região de Uberlândia-MG, Brasil

The success of the pesticide application is subject to meteorological conditions. The objective of this work was to determine the application windows suitable for carrying out the application of pesticides, using as parameters the analysis of historical data (2009-2014) of air temperature, relative air humidity, wind speed, and rainfall of Uberlândia-MG. With an electronic spreadsheet, and using the ideal meteorological conditions for applying pesticides (an air temperature above 30°C, relative air humidity above 55%, wind speed between 3 and 12 km h-1, and rainfall data lower than 0.2 mm h-1) as a parameter, the data were crossed with the aim to determine the times of day that weather conditions were ideal to obtain better efficiency in the application. According to the results, the relative air humidity was the main restricting criterion for the application windows, which are concentrated, on average, between 18:00 and 12:00 in the summer, and between 24:00 and 9:00 in the winter. The number of viable hours for a good application varied from 35 in the first fortnight of September to 198 in the second fortnight of March, demonstrating the great variability among the application windows throughout the year, which makes the generic recommendations of spraying time inadequate if the month of the year is not taken into consideration.

O sucesso das aplicações de produtos fitossanitários está condicionado às condições meteorológicas. Com este trabalho objetivou-se determinar as janelas de aplicação adequadas para a realização das aplicações de produtos fitossanitários, tendo como parâmetros a análise dos dados históricos da temperatura e umidade relativa do ar, velocidade do vento e ocorrência de chuva, para a região de Uberlândia-MG, no período de 2009 a 2014. Com o auxílio de uma planilha eletrônica, e tendo como parâmetro as condições meteorológicas ideais para a realização das aplicações de fitossanitários (temperatura abaixo de 30°C, umidade relativa acima de 55%, velocidade do vento entre 3 e 12 km h-1 e dados de pluviosidade menores que 0,2 mm h-1), fez-se o cruzamento dos dados com o intuito de verificar em quais horas do dia as quatro condições climáticas estavam dentro dos intervalos recomendados para se ter uma melhor eficiência nas aplicações. De acordo com os resultados, pôde-se concluir que a umidade relativa do ar foi o critério principal de restrição para as janelas de aplicação, que estão concentradas, em média, entre as 18 h e 12 h no verão, e entre as 24 h e 9 h no inverno. O número de horas viáveis para uma boa aplicação variou de 35 na primeira quinzena de setembro a 198 na segunda quinzena de março, demonstrando a grande variabilidade das janelas de aplicação ao longo do ano, o que torna as recomendações genéricas de horário para pulverização inadequadas se não for levado em consideração o mês do ano.

Solar radiation estimated from empirical models for the north of Minas Gerais, Brazil / Radiação solar estimada por modelos empíricos para o norte de Minas Gerais, Brasil

Estimating daily solar radiation (Rs) provides an important alternative in situations where it cannot be measured by conventional pyranometers. This study used meteorological data from nine cities in the north of the Minas Gerais state, Brazil, for the period from 2008 to 2010 with the aim of evaluate the accuracy and applicability of some simple models to help regions where Rs is impossible to be measured. Five models were evaluated for their estimates of Rs based on simple available data. For each city studied, the equations were previously calibrated. Meteorologically based empirical models to estimate daily global solar radiation are an appropriate tool if the parameters can be calibrated for different locations. These models have the advantage of using meteorological data, which are commonly available. Based on the overall results, we conclude that the accuracy of estimation by available meteorological data is acceptable and comparable with the accuracy of classical models. Considering the greater availability of air temperature data and application in studies that do not require great accuracy in estimating Rs, all models were adequate for use. The accuracy of Rs was only slightly improved by adding rainfall records as input variable. Therefore, in the region studied, the choice of simpler models, having as input the daily maximum and minimum air temperature would not imply large error in the estimates. For most sites, Bristow and Campbell model had the best estimate of Rs with a RMSE of 2.69 MJ m-2 and R2= 0.69, with the possibility to calibrate with available temperature data, becoming a practical and reliable model. Hargraves model should be avoided due to its lower performance compared to the other models applied.

A estimativa da radiação solar diária (Rs) fornece uma alternativa importante em situações que não pode ser medida por piranômetros convencionais. O estudo utilizou dados meteorológicos de nove cidades do Norte do estado de Minas Gerais, Brasil, durante o período de 2008 a 2010, com o objetivo de mensurar a precisão e aplicabilidade de modelos empíricos simples nas regiões onde a Rs não pode ser medida . Cinco modelos foram avaliados para estimar Rs com base nos dados meteorológicos disponíveis. As equações foram previamente calibradas para cada município estudado. Modelos meteorológicos empíricos que estimam a radiação solar diária são ferramentas adequadas desde que os parâmetros sejam calibrados para os diferentes locais a serem utilizados. Estes modelos têm a vantagem de utilizar dados meteorológicos, que estão comumente disponíveis. Todos os modelos foram considerados adequados para o uso, considerando-se a maior disponibilidade de dados de temperatura do ar e aplicação em estudos que não exigem grande precisão na estimativa da Rs. A precisão da Rs apenas foi melhorada pela adição de registros de precipitação como variável de entrada. Assim, na região estudada, a escolha de um modelo mais simples, tendo como entrada a temperatura mínima e máxima do ar diária, não implica um grande erro na estimativa. Para a maioria das regiões, o modelo de Bristow e Campbell teve a melhor estimativa da Rs com um RMSE de 2.69 MJ m-2 e R2= 0.69, e a possibilidade de calibração com os dados de temperatura disponíveis, tornando-se um modelo prático e confiável. O modelo de Hargraves deve ser evitado devido seu pior desempenho comparado aos outros modelos propostos.