Population structure associated with bioregion and seasonal prey distribution for Indo-Pacific bottlenose dolphins (Tursiops aduncus) in South Africa.

Many marine species exhibit fine-scale population structure despite high mobility and a lack of physical barriers to dispersal, but the evolutionary drivers of differentiation in these systems are generally poorly understood. Here we investigate the potential role of habitat transitions and seasonal prey distributions on the evolution of population structure in the Indo-Pacific bottlenose dolphin, Tursiops aduncus, off South Africa's coast, using double-digest restriction-site associated DNA sequencing. Population structure was identified between the eastern and southern coasts and correlated with the habitat transition between the temperate Agulhas (southern) and subtropical Natal (eastern) Bioregions, suggesting differentiation driven by resource specializations. Differentiation along the Natal coast was comparatively weak, but was evident in some analyses and varied depending on whether the samples were collected during or outside the seasonal sardine (Sardinops sagax) run. This local abundance of prey could influence the ranging patterns and apparent genetic structure of T. aduncus. These findings have significant and transferable management implications, most importantly in terms of differentiating populations inhabiting distinct bioregions and seasonal structural patterns within a region associated with the movement of prey resources.

The Natural History and Conservation of Indian Ocean Humpback Dolphins (Sousa plumbea) in South African Waters.

Although most knowledge on the biology of Sousa plumbea has primarily come from South African waters, a number of research gaps remain on the natural history and status of the species in the region. Research on two populations in South African waters for which some historical data exist may aid in highlighting long-term changes in the biology and natural history of this little known coastal delphinid. Recent studies on the age, growth and reproduction of animals incidentally caught in shark nets in Richards Bay, KwaZulu-Natal, yielded a lower maximum age estimate of 24 (previously 46) growth-layer-groups (GLGs), sexual maturity of 7.5 and 8 GLGs in males and females (previously 12-13 and 10 GLGs, respectively), an ovulation rate of 0.2 and a 5-year calving interval (previously 0.3 and 3-year calving interval) than previously reported. These differences may be due to a difference in the interpretation of GLGs between observers or a predominance of young males being caught in the shark nets. Stomach content analysis revealed a change in the relative proportions of the main prey items over the past 25 years, but no difference in species richness or diversity was found between the sexes. No change in trophic level was recorded between 1972 and 2009. Field studies in Algoa Bay, Eastern Cape, conducted 16 years apart indicated a decline in the mean group size (from 7 to 3 animals), a decline in the maximum group size (from 24 to 13 animals), an increase in solitary individuals (15.4-36%), and a change in behaviour from predominantly foraging (64-18%) to mainly travelling (24-49%). The observed changes are suggestive of a change in food availability, resulting in a range shift or a potential decline in numbers. These studies indicate the importance of long-term studies to monitor population changes and their possible causes. A number of threats, such as shark nets, pollution (noise and chemical), and coastal development and disturbance, to the humpback dolphin populations in South Africa have been identified. Urgent action is required to ensure continued existence of the species in South African waters.

Whistle vocalizations of Indo-Pacific bottlenose dolphins (Tursiops aduncus) inhabiting the south-west Indian Ocean.

Populations of Indo-Pacific bottlenose dolphin (Tursiops aduncus) are distributed along coastal regions of the south-west Indian Ocean (SWIO), from South Africa to Kenya. An account of whistles from wild T. aduncus inhabiting the SWIO is provided here. Recordings were made at Plettenberg Bay (South Africa) and Zanzibar Island (Tanzania) and the frequency trace of whistle contours (n = 1677) was extracted. Multiple parameters were measured from each whistle and compared between regions and encounters. Regional variation was significant in all parameters assessed except for start and middle frequency (frequency at half the duration). Whistles from Zanzibar Island ended on average 4 kHz higher than those from Plettenberg Bay, and had a steeper frequency gradient. However, mean frequencies differed by <1 kHz and population averages for the adopted frequency distribution showed similar patterns, with a peak between 5 and 7 kHz. Whistle parameters were strongly influenced by recording encounter, likely reflecting the presence of different individuals, group compositions and behavioral contexts during recording occasions. Comparisons within the genus showed that T. aduncus from the SWIO have amongst the lowest start and minimum frequency of whistles within Tursiops.

MTSEA prevents ligand binding to the human melanocortin-4 receptor by modification of cysteine 130 in transmembrane helix 3.

We have investigated the effect of the sulfhydryl-reactive reagent, methyl thiosulfonate ethylammonium (MTSEA), on ligand binding to the human melanocortin-4 (MC4) receptor stably expressed in HEK-293 cells. MTSEA inhibited binding of the agonist, 125I-NDPalpha-MSH, and the antagonist, 125I-SHU9119, in a concentration-dependent manner. Pre-incubation of cells with either the agonist or antagonist protected from subsequent MTSEA inhibition of radioligand binding. Mutation of Cys130 in transmembrane helix 3 to alanine, whilst not affecting ligand binding, led to a complete loss of the inhibitory effect of MTSEA. Since other types of sulfhydryl-reactive reagents had no effect on ligand binding, we conclude that covalent modification of Cys130 by MTSEA disrupts ligand binding by neutralising a close-by negative charge, most likely on Asp126.