RESUMEN
SUMMARY: Whole genome assembly (WGA) of bacterial genomes with short reads is a quite common task as DNA sequencing has become cheaper with the advances of its technology. The process of assembling a genome has no absolute golden standard and it requires to perform a sequence of steps each of which can involve combinations of many different tools. However, the quality of the final assembly is always strongly related to the quality of the input data. With this in mind we built WGA-LP, a package that connects state-of-the-art programs for microbial analysis and novel scripts to check and improve the quality of both samples and resulting assemblies. WGA-LP, with its conservative decontamination approach, has shown to be capable of creating high quality assemblies even in the case of contaminated reads. AVAILABILITY AND IMPLEMENTATION: WGA-LP is available on GitHub (https://github.com/redsnic/WGA-LP) and Docker Hub (https://hub.docker.com/r/redsnic/wgalp). The web app for node visualization is hosted by shinyapps.io (https://redsnic.shinyapps.io/ContigCoverageVisualizer/). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Programas Informáticos , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Análisis de Secuencia de ADN/métodos , Genoma BacterianoRESUMEN
A simple, specific, and sensitive high-performance liquid chromatographic method for the determination of papaveraldine and papaverinol in papaverine hydrochloride injection has been developed. The compounds were chromatographed on a reversed-phase C-18 column using a water-methanol-acetonitrile solvent system containing sodium lauryl sulphate ion-pair reagent.
RESUMEN
In continuation of earlier work on biguanide derivatives a number of 1-alkoxybenzensulphonyl-5-alkylbiguanides has been prepared. The U.V. absorption spectra have been measured and Beer's Law investigated. None of the compounds tested extensively for microbiological and pharmacological activity was found to possess significant activity in our screening.