An international study on the importance of androstenone and skatole for boar taint: I. Presentation of the programme and measurement of boar taint compounds with different analytical procedures.

An international study, involving 11 participants in 7 European countries, was conducted to provide scientific evidence for an objective measurement of boar taint in entire male pigs and its possible variation between countries. The specific objectives were to determine the respective contributions of androstenone and skatole to boar taint and their possible variations according to production systems and consumer populations. Over 4000 entire male pigs and 200 gilts were raised and slaughtered in 6 countries. Meat samples were taken from the loin and backfat samples were used for the rapid measurement of androstenone and skatole. A sub-population of 377 entire males and 42 gilts was then selected in such a way as to represent all combinations of skatole and androstenone levels. Androstenone and skatole levels in the selected samples were checked, using established reference methods. Meat samples from the selected animals were used for sensory evaluation by trained panels and for consumer surveys in 7 European countries. The present paper gives a general presentation of the programme and reports the main characteristics of the samples. Three companion papers present the results of the evaluation by trained sensory panels [Dijksterhuis, G., Engel, B., Walstra, P., Font i Furnols, M., Agerhem, H., Fisher, K., Oliver, M. A., Claudi-Magnussen, C., Siret, F., Béague, M. P., Homer, D. B., & Bonneau, M. (2000). An international study on the importance of androstenone and skatole for boar taint: II. Sensory evaluation by trained panels in seven European countries. Meat Science54, 261-269], the results of the consumer surveys [Matthews, K. R., Homer, D. B., Punter, P., Béague, M. P., Gispert, M., Kemspter, A. J., Agerhem, H., Claudi-Magnussen, C., Fischer, K., Siret, F., Leask, H., Font i Furnols, M., & Bonneau, M. (2000). An international study on the importance of androstenone and skatole for boar taint: III. Consumer survey in seven European countries. Meat Science, 54, 271-283] and the main conclusions and recommendations [Bonneau, M., Walstra, P., Claudi-Magnussen, C., Kempster, A. J., Tornberg, E., Fischer, K., Diestre, A., Siret, F., Chevillon, P., Claus, R., Dijksterhuis, G., Punter, P., Matthews, K. R., Agerhem, H., Béague, M. P., Oliver, M. A., Gispert, M., Weiler, U., von Seth, G., Leask, H., Font i Furnols, M., Homer, D. B., & Cook, G. L. (2000). An international study on the importance of androstenone and skatole for boar taint: IV. Simulation studies on consumer dissatisfaction with entire male pork and the effect of sorting out carcasses on the slaughter line, main conclusions and recommendations. Meat Science, 54, 285-295]. Seasonal effects and differences between countries in skatole and androstenone levels are presented elsewhere [Walstra, P., Claudi-Magnussen, C., Chevillon, P., von-Seth, G., Diestre, A., Matthews, K. R., Homer, D. B., & Bonneau, M. (in press). An international study on the importance of androstenone and skatole for boar taint: Levels of androstenone and skatole according to country and season. Livestock Production Science]. A supplementary paper considers the effects of human sensitivity to androstenone and skatole on the consumer acceptance of entire male pork [Weiler, U., Font i Furnols, M., Fischer, K., Kemmer, H., Oliver, M. A., Gispert, M., Dobrowolski, A., & Claus, R. (in press). Influence of differences in sensitivity of Spanish and German consumers to perceive androstenone on the acceptance of boar meat differing in skatole and androstenone concentrations. Meat Science]. A study of possible other compounds contributing to boar taint was also carried out within this programme.

An international study on the importance of androstenone and skatole for boar taint: IV. Simulation studies on consumer dissatisfaction with entire male pork and the effect of sorting carcasses on the slaughter line, main conclusions and recommendations.

An international study has been conducted in order to determine the respective contributions of androstenone and skatole to boar taint and their possible variations according to production systems and consumer populations. The presentation of the study and the main results concerning skatole and androstenone levels and data from sensory evaluation or consumer surveys are reported in companion papers. The present paper summarises the main conclusions of the study and gives tentative recommendations. A simulation study was conducted, based on the skatole and androstenone levels currently observed in European populations of entire male pigs and on the results of the consumer surveys. The first part of the simulation study demonstrated that, overall, 6.5% (odour) and 3.0% (flavour) more consumers were dissatisfied with entire male than with gilt pork. The differences were, however, very variable according to countries. Consumer dissatisfaction for the odour of entire male pork was mostly associated with high skatole levels, while androstenone had little influence on it. On the other hand, androstenone and skatole had similar contributions to the level of dissatisfaction for flavour. From the present study it is not possible to determine clear cut-off levels for androstenone/skatole. The regression equations presented in [Matthews, K. R., Homer, D. B., Punter, P., Béague, M. P., Gispert, M., Siret, F., Leask, H., Fonti i Furnols, M., & Bonneau, M. (2000). An international study on the importance of androstenone, skatole for boar taint: III. Consumer survey in seven European countries. Meat Science, 54, 271-283] provide a basis for decision making. However, due to methodological limitations, the results may underestimate consumer reaction to entire male pork. The second part of the simulation study demonstrated that sorting carcasses on the basis of androstenone/skatole would reduce, but not eliminate, differences in consumer dissatisfaction between entire male and gilt pork. For odour, taking androstenone into account did not improve the efficiency obtained from sorting using skatole only. For flavour, sorting using both compounds was more efficient than sorting using skatole only. Sorting out 15% of the entire males, on the basis of skatole only, would result in a difference in the proportion of dissatisfied consumers of 4.2% (odour) or 2.0% (flavour) between entire male and gilt pork. The results of the last part of the simulation study demonstrated that decreasing skatole in entire male pig populations, to levels as low as 0.10 ppm, would still result in a difference in the proportion of dissatisfied consumers of 3.2% (odour) or 1.6% (flavour). To reduce this difference further, the levels of both compounds would have to be reduced still further. The lowest difference that can be achieved is 2.3% (odour) or 0.4% (flavour). The conclusions of the present study may differ according to whether immediate commercial applications or long-term goals are considered. On the basis of the skatole and androstenone levels currently observed in entire male pig populations, sorting out procedures based on skatole is the easiest way to rapidly achieve a significant decrease in consumer dissatisfaction with entire male pork. In most countries, however, this will not be sufficient to obtain the same level of acceptability as with gilts. In the long term, a sharp reduction in both skatole and androstenone would have to be achieved in entire male pig populations to obtain low differences in acceptability between entire male and gilt pork.

Valuing of identity, distribution of attention, and perceptual salience in free and rule-governed classifications.

This experiment investigated classifications of multidimensional objects to test whether (1) the valuing of identity as a classification criterion occurs early in development, (2) the distribution of attention to multiple relations increases with development, and (3) the role of separate, component relations in solving multidimensional classification problems would be manifested through measures of perceptual salience. A salience preassessment followed by free and rule-governed classification tasks were given to 4-, 7-, and 18-year-olds. Total identity, partial identity, overall similar, or overall different choices were available for classification. Results associated with both free and rule-governed classifications indicated that identity serves as a highly valued classification criterion beginning as early as 4 years of age. Results associated with salience indicated that children as young as 4 years perceive and process relations separately and that 4-year-olds show greater differential attention than older children and adults.

Perception of multidimensional stimuli: a differential-sensitivity account of cognitive processing and development.

A differential-sensitivity account of cognitive processing is described that emphasizes the development of perceptual sensitivity to object relations that are directly perceived. Four experiments are presented that investigate this account and compare it to the integrality-separability view of development and the view that younger children are nonselective in their processing of multidimensional stimuli. Results show that stimulus differences are more salient than identities (Expt. 1), younger as well as older children are highly selective in their perception and classify stimuli by separate dimensions (Expt. 2), differential sensitivity affects the perceived magnitude of stimulus differences (Expt. 3), and younger and older children perceive separate dimensions in speeded classifications (Expt. 4). The importance of considering individual patterns of responses in cognitive and developmental research is also demonstrated.

Incorporation of a stable isotopically labeled amino acid into multiple human apolipoproteins.

Procedures are presented for the separation and determination of the isotopic enrichment of multiple human apolipoproteins labeled in vivo with a stable isotope amino acid. The isotopic enrichments of plasma lysine and plasma apolipoproteins were monitored for 16 days after a single intravenous dose of [4,4,5,5-2H4]lysine (5 mg/kg body weight). The use of a multiply deuterated amino acid enabled the measurement of isotopic enrichments above background over the entire 16-day time course in all proteins. Individual apolipoproteins were separated on a specially designed gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis system cast in a conventional slab gel apparatus which resolved apoB-100, apoE, apoA-I, apoA-II, apoC-I, apoC-II, apoC-III-1, and apoC-III-2 on a single gel. After staining with Coomassie blue, proteins bands (containing 5 to 30 micrograms of individual apolipoprotein) were excised from the gel. Amino acids were recovered from hydrolyzed gel slices, derivatized, and analyzed by gas chromatography-mass spectrometry for determination of lysine isotopic enrichments. The utility of the method is demonstrated using examples of apolipoproteins B-100, A-I, A-II, C-I, C-II, and C-III from either total plasma d less than 1.21 g/ml lipoproteins or selected lipoprotein subfractions. Lysine isotopic enrichments of proteins were generally determined with a precision of better than 5%. The isotopic enrichment profiles were consistent with literature reports of apolipoprotein metabolic kinetics based on the use of radioiodinated apolipoproteins. The procedures outlined can be used to separate and measure the isotopic enrichment of virtually any apolipoprotein from any chosen lipoprotein fraction. Thus, these procedures should find wide application in the study of apolipoprotein metabolic kinetics.

Perceptual sensitivity to dimensional and similarity relations in free and rule-governed classifications.

Two experiments were conducted to investigate the perceptual primacy of dimensional and similarity relations in the stimulus classifications of younger and older subjects. In Experiment 1, 4- and 10-year-olds were given free classifications in which they could group stimuli according to overall similarity or identities in size, color, or orientation. Both age groups classified stimuli most frequently according to identities on separate dimensions. In Experiment 2, 4-year-olds and adults were given free classifications followed by rule-governed classifications which required them to group stimuli according to specific relations. In the free classifications, a majority of subjects in both age groups classified the stimuli most frequently according to identities on separate dimensions. In the rule-governed classifications, both age groups were more accurate when a single separate relation was required for solution than when overall similarity was required. These results support a differential-sensitivity view of perceptual development, which asserts that individuals at all ages primarily perceive and use separate relations.

National estimates of the body composition of British cattle, sheep and pigs with special reference to trends in fatness. A review.

National carcass classification records for cattle, sheep and pigs were used as a basis for estimating the body composition of British livestock slaughter populations. The tissue percentages of carcasses in each classification fatness range were estimated from regression equations constructed using data from breed comparison trials and other sources. The lipid and protein contents of the tissues were then estimated using regression equations from body composition studies. The mean carcass composition, and lean and fat production from current populations (1984) were compared with those of ten years ago. There has been an important increase of 20 kg (8%) in carcass weight for beef but no change in carcass composition. The average beef carcass in 1984 was estimated to contain 23% lipid (inclusive of lipid in perinephric and retroperitoneal fat). The weight of sheep carcasses has fallen by 0·6 kg (3 1 2% ) since 1977 without any appreciable change in carcass composition. The average sheep carcass in 1984 was estimated to contain 24% lipid. The lipid content of the average pig carcass was estimated to have fallen significantly from 27% in 1975 to 22% in 1984, with a small increase in carcass weight.

Variation among beef breeds in carcass retail value at constant carcass saleable meat content and percentage of higher priced cuts.

Data from the single slaughter point trials of the Meat and Livestock Commission's (MLC) beef breed evaluation programme were used to examine the importance of retail value (RV) calculation in breed evaluation trials. The analysis involved a total of 2010 carcasses comprising four production system groups. Sire breeds included Aberdeen-Angus, British Friesian, Charolais, Devon, Hereford, Galloway, Limousin, Lincoln Red, Luing, Simmental, South Devon, Sussex and Welsh Black. RV was computed as the sum of the values of trimmed deboned primal joints expressed as pence per kilogram of carcass weight. 1981 prices from MLC's Retail Prices Survey for England and Wales were used. A model was fitted to the RV data for each of the production system groups with effects for sire breed, year and sire breed × year interaction, and with regression on saleable meat percentage in carcass (SM). The largest difference recorded between sire breeds was 2·1 pence/kg (0·85% of overall mean RV). The pooled within-system residual standard deviation after fitting the model was 1·4 pence/kg. When regression on the percentage of total saleable meat in the higher priced cuts (SMD) was added to the model, the corresponding range and residual standard deviation were reduced to 1·0 and 0·8 pence/kg, respectively. In the circumstances, the calculation of RV would, therefore, make only a minor refinement to the results.

Observations on a respiratory disease outbreak in weaned suckled calves.

Outbreaks of respiratory disease with a similar temporal pattern occurred in both autumn and spring-born groups of suckler calves transported from all parts of Great Britain to breed evaluation centre. Thirteen per cent (18 of 137) of the autumn-born group and 15 per cent (22 of 147) of the spring-born group were treated for respiratory disease. Most disease occurred within three weeks of an animal's entry to the centre. No disease occurred within certain breed groups of both age groups. There was a tendency for distance travelled to affect the need for treatment.

Tumoricidal response induced by cytosine arabinoside after plasma perfusion over protein A.

In dogs with spontaneous mammary adenocarcinomas, a single nontoxic infusion of cytosine arabinoside after extracorporeal perfusion of plasms over immobilized protein A resulted in a necrotizing response rapid in onset and specific for tumorous tissue. Gross tumoricidal reactions 12 hours after this combined treatment exceeded the algebraic sum of responses to cytosine arabinoside and protein A perfusion treatments alone in the same dogs, implying a synergistic effect between the two. The magnitude, rapidity, and specificity of the tumoricidal response after the combined treatment suggests that it may be an effective chemimmunotherapeutic approach to breast adenocarcinoma.

The mitochondrial activation of silicate and its role in silicosis, black lung disease and lung cancer.

Silicate substitutes for phosphate in the transitory uncoupling of rat liver mitochondria induced by hydrazine when beta-hydroxy-butyrate is the substrate. Uncoupling is blocked by rutamycin. Just as in the case when phosphate is combined with hydrazine, ATP, ADP, PPi, and Mg++ protect against hydrazine when silicate is combined with hydrazine. A high level of ADP in the absence of added phosphate, but in the presence of silicate, induces a pseudo state three of the mitochondria. Silicate, like sulfate and arsenate which have been reported previously, is activated by the enzymes which mediate oxidative phosphorylation. These results serve to explain a role for silicate in silicosis, black lung disease, and cancer. In addition, since there is suggestive evidence in the literature that lung tissue solubilizes asbestos fibers, these results not only expand the confluence between oxidative phosphorylation and chemical carcinogenesis but are correlated with the synergistic carcinogenicity of asbestos and smoking observed by epidemiologists.

The mitochondrial activation of sulfate and arsenate and their role in carcinogenesis.

Sulfate substitutes for phosphate in the transitory uncoupling of rat liver mitochondria induced by hydrazine when beta-hydroxybutyrate is the substrate. A high level of sulfate in the absence of added phosphate induces a pseudo state three of the mitochondria. Uncoupling is inhibited by rutamycin. Thus sulfate is activated by the mechanism usually utilized by phosphate, and the target for hydrazine is the bond holding electrophilic sulfate. ATP, ADP, PPi, and Mg++ protect against hydrazine, presumably by causing a conformational change of the phosphorylating enzymes which participate in oxidative phosphorylation. Arsenate also could substitute for phosphate in the transitory uncoupling induced by hydrazine. Uncoupling is again inhibited by rutamycin; thus arsenate is also activated by the enzymic mechanism usually utilized by phosphate. Since sulfate is known to enhance the carcinogenicity of certain carcinogens, these results expand the experimental confluence between oxidative phosphorylation and chemical carcinogenesis and also serve to explain at least in part the "toxic" effects of sulfate. Because of the analogous results with arsenate and sulfate, it is suggested that arsenate, like sulfate, may enhance the carcinogenicity of other carcinogens. The data are compatible with epidemiological studies which implicate some role in carcinogenesis for sulfate (often measured as a sulfur dioxide equivalent) and arsenate.

A requirement of Pi for the transitory uncoupling of rat liver mitochondria by hydrazine, when beta-hydroxybutyrate is the substrate.

We have recognized an experimental confluence between oxidative phosphorylation and chemical carcinogenesis and, therefore, became interested in the mitochondrial target of hydrazine, which is not only a potential environmental hazard as a carcinogen but is also a likely metabolite of many drugs. Hydrazine induced a Pi dependent transitory uncoupling of rat liver mitochondria when beta-hydroxybutyrate was the substrate. Uncoupling was inhibited by rutamycin; accordingly, the mitochondrial target for nucleophilic hydrazine is an electrophilic site, presumably involving activated Pi. The protective action of ATP2, ADP, PPi and Mg++ was attributed to a conformational change of the phosphorylating enzyme which participated in oxidative phosphorylation. In a mitochondrial system which included ATP gramicidin potassium ion and sulfate, hydrazine, acting as a large cation but not as a nucleophile, blocked mitochondrial swelling and the increment in ATPase activity associated with potassium ion. These data in conjunction with our previous reports dealing with other carcinogens and certain of their derivatives also contribute to an experimental confluence between oxidative phosphorylation and chemical carcinogenesis and are compatible with toxic effects of hydrazine on mitochondria observed previously by others.