Do Activities Performed within the Intra-Contrast Rest Interval Affect Neuromuscular Performance during Complex-Contrast Training Protocols?

The aim of this study was to analyze the acute effects of including different exercises within the intra-contrast rest interval (ICRI) of a complex-contrast training (CCT) session. Seventeen recreationally active males completed three different CCT protocols. Programs consisted of a contrast pair combining a moderate-intensity conditioning activity (i.e., a back squat) with a lower-body high-velocity exercise (i.e., a vertical jump) and only differed in the activities performed during the ICRI: 1) passive recovery (CCTPASS); 2) a mobility exercise (CCTMOB); and 3) an upper-body high-intensity strength exercise (i.e., a bench press) (CCTSTR). Countermovement jump and bench press throw metrics were evaluated at baseline and after each set during the workout. The rate of perceived exertion was recorded post-session. Non-significant differences in performance were found between CCTPASS, CCTMOB and CCTSTR throughout the session. Significant declines (p < 0.05) were observed for CMJ peak power in the last 2-3 repetitions of each set, irrespective of the protocol. CCTSTR was perceived as more intense than CCTPASS and CCTMOB (p < 0.05). From a neuromuscular performance perspective, including activities during the ICRI (mobility drills or high-intensity strength exercises) may be a suitable strategy to optimize CCT prescription since the acute responses were similar to those found with passive rest periods. Finally, prescribing a lower number of repetitions per set is recommended to attenuate mechanical performance impairment during CCT protocols, irrespective of the activities completed within the ICRI.

A Systematic Review and Meta-Analysis of Wearable Satellite System Technology for Linear Sprint Profiling: Technological Innovations and Practical Applications.

ABSTRACT: Cormier, P, Meylan, C, Agar-Newman, D, Geneau, D, Epp-Stobbe, A, Lenetsky, S, and Klimstra, M. A systematic review and meta-analysis of wearable satellite system technology for linear sprint profiling: technological innovations and practical applications. J Strength Cond Res 38(2): 405-418, 2024-An emerging and promising practice is the use of global navigation satellite system (GNSS) technology to profile team-sports athletes in training and competition. Therefore, the purpose of this narrative systematic review with meta-analysis was to evaluate the literature regarding satellite system sensor usage for sprint modeling and to consolidate the findings to evaluate its validity and reliability. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, an electronic search of the databases, PubMed and SPORTDiscus (EBSCO), was conducted. Concurrent validity and reliability studies were considered, and 16 studies were retained for the review from the initial 1,485 studies identified. The effects on outcomes were expressed as standardized mean differences (SMDs, Cohen's d ) for each outcome (i.e., maximal sprint speed [MSS], the acceleration constant [τ], maximal theoretical velocity [ V0 ], relative force [ F0 ], and relative power [P max ]). Effect magnitudes represented the SMD between GNSS-derived and criterion-derived (i.e., radar and laser) and resulted in the following estimates: small for MSS ( d = 0.22, 95% CI 0.01 to 0.42), τ ( d = -0.18, 95% CI -0.60 to 0.23), V0 ( d = 0.14, 95% CI -0.08 to 0.36), relative F0 ( d = 0.15, 95% CI -0.25 to 0.55), and relative P max ( d = 0.21, 95% CI -0.16 to 0.58). No publication bias was identified in meta-analyzed studies and moderator analysis revealed that several factors (sampling rate and sensor manufacturer) influenced the results. Heterogeneity between studies was considered moderate to high. This highlighted the differences between studies in sensor technology differences (i.e., sampling rate, sensor fusion, and satellite network acquisition), processing techniques, criterion technology used, sprint protocols, outcome reporting, and athlete characteristics. These findings may be useful in guiding improvements in sprint modeling using GNSS technology and enable more direct comparisons in future research. Implementation of all-out linear sprint efforts with GNSS technology can be integrated into sport-specific sessions for sprint modeling when robust and consistent data processing protocols are performed, which has important implications for fatigue monitoring, program design, systematic testing, and rehabilitation in individual and team sports.

A Simple and Valid Method to Calculate Wheelchair Frame Rotation Using One Wheel-Mounted IMU.

Wheelchair sports have been using Inertial Measurement Units (IMU) to measure mobility metrics during training, testing and competition. Presently, the most suitable solution to calculate wheelchair speed and frame rotation is the 3IMU method as there is uncertainty about the ability of a one wheel-mounted IMU (1IMU) approach to calculate wheelchair frame rotational kinematics. A new method for calculating wheelchair frame rotational kinematics using a single wheel-mounted IMU is presented and compared to a criterion measurement using a wheelchair-frame-mounted IMU. Goodness-of-fit statistics demonstrate very strong linear relationships between wheelchair frame angular velocity calculated from the wheel-mounted IMUs and a wheelchair-frame-mounted IMU. Root mean square error (RMSE), mean absolute error (MAE) and Bland-Altman analysis show very small differences between the wheelchair frame angular velocity calculated from the wheel-mounted IMUs and the wheelchair-frame-mounted IMU. This study has demonstrated a simple and accurate approach to estimating wheelchair frame rotation using one wheel-mounted IMU during an elite wheelchair athlete agility task. Future research is needed to reexamine and compare wheelchair mobility metrics determined using the 3IMU and 1IMU solutions using this new approach.

Wheelchair Rugby Sprint Force-Velocity Modeling Using Inertial Measurement Units and Sport Specific Parameters: A Proof of Concept.

BACKGROUND: Para-sports such as wheelchair rugby have seen increased use of inertial measurement units (IMU) to measure wheelchair mobility. The accessibility and accuracy of IMUs have enabled the quantification of many wheelchair metrics and the ability to further advance analyses such as force-velocity (FV) profiling. However, the FV modeling approach has not been refined to include wheelchair specific parameters. PURPOSE: The purpose of this study was to compare wheelchair rugby sprint FV profiles, developed from a wheel-mounted IMU, using current mono-exponential modeling techniques against a dynamic resistive force model with wheelchair specific resistance coefficients. METHODS: Eighteen athletes from a national wheelchair rugby program performed 2 × 45 m all-out sprints on an indoor hardwood court surface. RESULTS: Velocity modelling displayed high agreeability, with an average RMSE of 0.235 ± 0.07 m/s-1 and r2 of 0.946 ± 0.02. Further, the wheelchair specific resistive force model resulted in greater force and power outcomes, better aligning with previously collected measures. CONCLUSIONS: The present study highlights the proof of concept that a wheel-mounted IMU combined with wheelchair-specific FV modelling provided estimates of force and power that better account for the resistive forces encountered by wheelchair rugby athletes.

Minimal Number of Events Required for Acceleration-Speed Profiling in Elite Women's Soccer.

PURPOSE: To determine the minimum number of events (training or matches) for producing valid acceleration-speed (AS) profiles from global navigation satellite system (GNSS) data. METHODS: Nine elite female soccer players participated in a 4-week training camp consisting of 19 events. AS profile metrics calculated from different combinations of athlete events were compared to force-velocity (FV) profile metrics from 2 × 40-m stand-alone sprint effort trials, using the same GNSS 10-Hz technology. Force-velocity profiles were calculated, from which AS profiles were obtained. AS profiles from training and matches were generated by plotting acceleration and speed points and performing a regression through the maximal points to obtain the AS metrics (theoretical maximal speed, x-intercept [in meters per second], theoretical maximal acceleration, y-intercept [in meters per second squared], and the slope per second). A linear mixed model was performed with the AS metrics as the outcome variables, the number of events as a fixed effect, and the participant identifier as a mixed effect. Dunnett post hoc multiple comparisons were used to compare the means of each number of event grouping (1-19 events) to those estimated from the dedicated sprint test. RESULTS: Theoretical maximal speed and theoretical maximal acceleration means were no longer significantly different from the isolated sprint reference with 9 to 19 (small to trivial differences = -0.31 to -0.04 m·s-1, P = .12-.99) and 6 to 19 (small differences = -0.4 to -0.28 m·s-2, P = .06-.79) events, and the slopes were no longer different with 1 to 19 events (trivial differences = 0.06-0.03 s-1, P = .35-.99). CONCLUSIONS: AS profiles can be estimated from a minimum of 9 days of tracking data. Future research should investigate methodology resulting in AS profiles estimated from fewer events.

Comparison of acceleration-speed profiles from training and competition to individual maximal sprint efforts.

This study aimed to (1) compare "in-situ" monitored acceleration-speed (ASin-situ) profile metrics from training/competition data in elite female soccer players to similar metrics from profiles developed from isolated maximal sprint efforts (ASsprint) and; (2) compare the confidence interval (CI) and a Tukey boxplot (BP) outlier removal technique on the training/competition data to derive ASin-situ profiles. Fifteen national team soccer players participated in a 4-week camp while wearing 10 Hz GNSS units. Towards the middle of the camp, 2 × 40 m isolated maximal sprints were performed. ASin-situ profiles (theoretical maximum acceleration A0 in m∙s-2 and speed S0 in m∙s-1) were computed using the CI and BP techniques with training/competition data. The sprint data were modelled separately to construct horizontal force-velocity (FV) profiles, from which ASsprint profiles were derived. Bland-Altman analysis was used to assess agreement between the CI- and BP-derived ASin-situ profiles to the ASsprint profiles, as well as regression analysis for systematic and proportional bias. Additionally, 1-way ANOVAs with Tukey posthoc compared the metrics between each method of analysis. Using the BP method, good agreement of the ASin-situ with ASsprint profile metrics A0/S0 was displayed, whereas good to moderate agreement was shown for the CI. The CI technique showed a proportional bias for A0/S0. Good to excellent intertrial reliability was demonstrated for isolated sprint metrics. Both BP and CI techniques provided comparable ASin-situ profiles to ASsprint profiles. This current research demonstrated that ASin-situ profiling is applicable in elite women's soccer and will have further application in many team sports.

Analysis of the P. lividus sea urchin genome highlights contrasting trends of genomic and regulatory evolution in deuterostomes.

Sea urchins are emblematic models in developmental biology and display several characteristics that set them apart from other deuterostomes. To uncover the genomic cues that may underlie these specificities, we generated a chromosome-scale genome assembly for the sea urchin Paracentrotus lividus and an extensive gene expression and epigenetic profiles of its embryonic development. We found that, unlike vertebrates, sea urchins retained ancestral chromosomal linkages but underwent very fast intrachromosomal gene order mixing. We identified a burst of gene duplication in the echinoid lineage and showed that some of these expanded genes have been recruited in novel structures (water vascular system, Aristotle's lantern, and skeletogenic micromere lineage). Finally, we identified gene-regulatory modules conserved between sea urchins and chordates. Our results suggest that gene-regulatory networks controlling development can be conserved despite extensive gene order rearrangement.

Concurrent Validity and Reliability of Different Technologies for Sprint-Derived Horizontal Force-Velocity-Power Profiling.

ABSTRACT: Cormier, P, Tsai, M-C, Meylan, C, Agar-Newman, D, Epp-Stobbe, A, Kalthoff, Z, and Klimstra, M. Concurrent validity and reliability of different technologies for sprint-derived horizontal force-velocity-power profiling. J Strength Cond Res 37(6): 1298-1305, 2023-This study evaluated the validity and reliability of common systems to assess sprint-derived horizontal force-velocity-power ( FVPH ) profile metrics. Two double constellation athlete monitoring systems (STATSports Apex, Catapult Vector S7) and one timing gate system were compared with a radar gun for the computation of FVPH metrics. Intersystem validity was assessed using intraclass correlation coefficients (ICC), Pearson's correlation coefficients ( R2 ), and Bland-Altman plots with absolute and percent agreement. Intrasystem reliability was assessed with agreement bias and ICC. STATSports demonstrated moderate agreement for F0 , Pmax , τ, and Drf (8.62, 6.46, -9.81, and 9.96%, respectively) and good agreement for V0 and MSS (-2.18 and -1.62%). Catapult displayed good agreement across all metrics ( F0 , V0 , Pmax , MSS, τ, and Drf : -0.96, -0.89, -1.85, -0.84, 0.38, and -0.27%, respectively). Timing gates demonstrated good agreement with V0 and MSS (-2.62 and -1.71%) and poor agreement with F0 , Pmax , τ, and Drf (19.17, 16.64, -20.49, and 20.18%, respectively). Intrasystem reliability demonstrated good agreement (<2% bias) with very large to near-perfect ICC (0.84-0.99) for Catapult and STATSports systems. Overall, GPS/GNSS 10 Hz technology is reliable across devices and can provide moderate-to-good accuracy of FVPH metrics in single maximal effort sprints. However, Catapult provided better agreement for more FVPH metrics than STATSports, which may be related to differences in proprietary algorithms. Also, modeling timing gate data using current FVPH profiling techniques results in poor bias that requires greater investigation. GPS/GNSS data can be used for FVPH profiling, which could inform performance and rehabilitation processes.

eIF4B mRNA Translation Contributes to Cleavage Dynamics in Early Sea Urchin Embryos.

During the first steps of sea urchin development, fertilization elicits a marked increase in protein synthesis essential for subsequent cell divisions. While the translation of mitotic cyclin mRNAs is crucial, we hypothesized that additional mRNAs must be translated to finely regulate the onset into mitosis. One of the maternal mRNAs recruited onto active polysomes at this stage codes for the initiation factor eIF4B. Here, we show that the sea urchin eIF4B orthologs present the four specific domains essential for eIF4B function and that Paracentrotus lividus eIF4B copurifies with eIF4E in a heterologous system. In addition, we investigated the role of eIF4B mRNA de novo translation during the two first embryonic divisions of two species, P. lividus and Sphaerechinus granularis. Our results show that injection of a morpholino directed against eIF4B mRNA results in a downregulation of translational activity and delays cell division in these two echinoids. Conversely, injection of an mRNA encoding for P. lividus eIF4B stimulates translation and significantly accelerates cleavage rates. Taken together, our findings suggest that eIF4B mRNA de novo translation participates in a conserved regulatory loop that contributes to orchestrating protein synthesis and modulates cell division rhythm during early sea urchin development.

Within Session Exercise Sequencing During Programming for Complex Training: Historical Perspectives, Terminology, and Training Considerations.

The primary aim of this narrative review was to outline the historical genesis of resistance training strategies that incorporate high-load, low-velocity exercises and low-load, high-velocity exercises in the same training session allowing for different "exercise sequences" to be simultaneously implemented. Discrepancies between scientific works and the terminology used within contemporary sport science publications are identified. Upon review of the literature, we propose "complex training" to be considered an umbrella term with 4 different implementations, generally used to indicate a method in which movement velocity or load is altered between sets and/or exercises within the same session with the aim of improving slow and fast force expression. We propose the following terminology for said implementations: contrast training-exercise sequence with alternating high-load and low-load (higher-velocity) exercises in a set-by-set fashion within the same session (corresponding with 'contrast pairs' and 'intra-contrast rest'); descending training-several sets of high-load (e.g., back squat) exercises completed before the execution of several sets of low-load, higher-velocity (e.g., vertical jump) exercises within the same session; ascending training-several sets of low-load, higher-velocity exercises completed before several sets of high-load exercises within the same session; and French contrast training-subset of contrast training in which a series of exercises are performed in sequence within a single session: heavy compound exercise, plyometric exercise, light-to-moderate load compound exercise that maximises movement speed (i.e., external power), and a plyometric exercise (often assisted). Finally, practical applications and training considerations are presented.

A systematic review of resistance training methodologies for the development of lower body concentric mean power, peak power, and mean propulsive power in team-sport athletes.

This study aimed to systematically review training methods prescribed to develop lower-body power, determine their effectiveness for the development of lower-body mechanical power and their implementation in an annual training cycle amongst team-sport athletes. The absolute and relative outcome values of concentric mean power, peak power and mean propulsive power were extracted from 19 studies. Outcomes were assessed using baseline to post intervention percent change, effect sizes, and the level of evidence concerning the method's effectiveness. A thorough analysis of the literature indicated that, based on the high level of evidence, traditional (e.g., strength training alone) and combination training (e.g., complex and contrast) methods should be considered. Further, optimal load and velocity-based training can be implemented if coaches have access to the appropriate equipment to monitor movement velocity and mechanical power in every session. This is of particular importance in periods of the season where high volumes of technical-tactical training and congested fixture periods are present. Also, flywheel, eccentric overload and weightlifting methods have been shown to be effective although the level of evidence is low. Future research should expand on current training practices whilst adequately reporting actual training loads from sport-specific training and games alongside strength-power training protocols.

mTOR Signaling at the Crossroad between Metazoan Regeneration and Human Diseases.

A major challenge in medical research resides in controlling the molecular processes of tissue regeneration, as organ and structure damage are central to several human diseases. A survey of the literature reveals that mTOR (mechanistic/mammalian target of rapamycin) is involved in a wide range of regeneration mechanisms in the animal kingdom. More particularly, cellular processes such as growth, proliferation, and differentiation are controlled by mTOR. In addition, autophagy, stem cell maintenance or the newly described intermediate quiescence state, Galert, imply upstream monitoring by the mTOR pathway. In this review, we report the role of mTOR signaling in reparative regenerations in different tissues and body parts (e.g., axon, skeletal muscle, liver, epithelia, appendages, kidney, and whole-body), and highlight how the mTOR kinase can be viewed as a therapeutic target to boost organ repair. Studies in this area have focused on modulating the mTOR pathway in various animal models to elucidate its contribution to regeneration. The diversity of metazoan species used to identify the implication of this pathway might then serve applied medicine (in better understanding what is required for efficient treatments in human diseases) but also evolutionary biology. Indeed, species-specific differences in mTOR modulation can contain the keys to appreciate why certain regeneration processes have been lost or conserved in the animal kingdom.

A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos.

The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.

Complex and Contrast Training: Does Strength and Power Training Sequence Affect Performance-Based Adaptations in Team Sports? A Systematic Review and Meta-analysis.

Cormier, P, Freitas, TT, Rubio-Arias, JÁ, and Alcaraz, PE. Complex and contrast training: Does strength and power training sequence affect performance-based adaptations in team sports? A systematic review and meta-analysis. J Strength Cond Res 34(5): 1461-1479, 2020-The aims of this meta-analysis were to examine the effects of 2 different strength and power training sequences (complex: CPX; and contrast: CNT, training) on performance-based adaptations in team sports {lower-body strength (1 repetition maximum [1RM]), vertical jump (VJ), sprinting, and change of direction (COD) ability}, as well as identify factors potentially affecting said adaptations (i.e., athlete level, type of sport, intensity, and duration). CPX is the combination training that alternates biomechanically similar high load weight training exercises with lighter load power exercises, set for set (e.g., squats followed by countermovement jumps). CNT is the combination training where all high load strength exercises are performed at the beginning of the session and all lighter load power exercises at the end. After an electronic database search (PubMed, SPORTDiscus, and WoS), a total of 27 articles were included in the meta-analysis. The effects on outcomes were expressed as standardized mean differences (SMDs). Baseline to postintervention overall results for the studied variables: (a) 1RM: large effects for CPX (SMD = 2.01, 95% confidence interval [CI] 1.18-2.84) and CNT (SMD = 1.29, 95% CI 0.61-1.98); (b) VJ: large effects for CPX (SMD = 0.88, 95% CI 0.42-1.34) and medium effects for CNT (SMD = 0.55, 95% CI 0.29-0.81); (c) sprint: large effects for CPX (SMD = -0.94, 95% CI -1.33 to -0.54) and small effects for CNT (SMD = -0.27, 95% CI -0.92 to 0.39); and (d) COD: large effects for CPX (SMD = -1.17, 95% CI -1.43 to -0.90) and medium effects for CNT (SMD = -0.68, 95% CI -1.20 to -0.15). Regarding the studies that contained a control group: (a) 1RM: large effects for CPX (SMD = 1.61, 95% CI 1.12-2.10) and CNT (SMD = 1.38, 95% CI 0.30-2.46); (b) VJ: large effects for CPX (SMD = 0.85, 95% CI 0.45-1.25) and medium for CNT (SMD = 0.50, 95% CI 0.19-0.81); (c) sprint: medium effects for CPX (SMD = -0.69, 95% CI -1.02 to -0.36) and CNT (SMD = -0.51, 95% CI -0.90 to -0.11); and (d) COD: large effects for CPX (SMD = -0.83, 95% CI -1.08 to -0.59), and there were no control groups for CNT. In conclusion, both training interventions may lead to positive performance-based adaptations in team-sports with CPX interventions potentially leading to slightly greater effects.

In vivo analysis of protein translation activity in sea urchin eggs and embryos.

Protein synthesis is a major regulatory step of gene expression in different physiological processes including development. Translation of proteins in sea urchin is stimulated upon fertilization and is necessary for cell cycle progression and development. Translational control is exerted through multifactorial mechanisms, including mRNA recruitment into polysomes and increased rates of translational activity. In this chapter, we review the methods used in sea urchin eggs and embryos to analyze translation activity in vivo both from perspectives of the proteins and of the mRNAs. First, we describe methods to quantify or visualize newly synthesized proteins with radioactive and non-radioactive labeling techniques. Next we present the polysome isolation and profiling on sucrose gradients, allowing the identification of translated mRNAs. Finally, we outline a procedure to follow the translation of a reporter luciferase protein from an mRNA microinjected into the egg.

Translational Control of Canonical and Non-Canonical Translation Initiation Factors at the Sea Urchin Egg to Embryo Transition.

Sea urchin early development is a powerful model to study translational regulation under physiological conditions. Fertilization triggers an activation of the translation machinery responsible for the increase of protein synthesis necessary for the completion of the first embryonic cell cycles. The cap-binding protein eIF4E, the helicase eIF4A and the large scaffolding protein eIF4G are assembled upon fertilization to form an initiation complex on mRNAs involved in cap-dependent translation initiation. The presence of these proteins in unfertilized and fertilized eggs has already been demonstrated, however data concerning the translational status of translation factors are still scarce. Using polysome fractionation, we analyzed the impact of fertilization on the recruitment of mRNAs encoding initiation factors. Strikingly, whereas the mRNAs coding eIF4E, eIF4A, and eIF4G were not recruited into polysomes at 1 h post-fertilization, mRNAs for eIF4B and for non-canonical initiation factors such as DAP5, eIF4E2, eIF4E3, or hnRNP Q, are recruited and are differentially sensitive to the activation state of the mechanistic target of rapamycin (mTOR) pathway. We discuss our results suggesting alternative translation initiation in the context of the early development of sea urchins.

Harmful or harmless: Biological effects of marennine on marine organisms.

Marennine is a water-soluble blue-green pigment produced by the marine diatom Haslea ostrearia. The diatom and its pigment are well known from oyster farming areas as the source of the greening of oyster gills, a natural process increasing their market value in Western France. Blooms of blue Haslea are also present outside oyster ponds and hence marine organisms can be exposed, periodically and locally, to significant amounts of marennine in natural environments. Due to its demonstrated antibacterial activities against marine pathogenic bacteria (e.g. Vibrio) and possible prophylactic effects toward bivalve larvae, marennine is of special interest for the aquaculture industry, especially bivalve hatcheries. The present study aimed to provide new insights into the effects of marennine on a large spectrum of marine organisms belonging to different phyla, including species of aquaculture interest and organisms frequently employed in standardised ecotoxicological assays. Different active solutions containing marennine were tested: partially purified Extracellular Marennine (EMn), and concentrated solutions of marennine present in H. ostrearia culture supernatant; the Blue Water (BW) and a new process called Concentrated Supernatant (CS). Biological effects were meanwhile demonstrated in invertebrate species for the three marennine-based solutions at the highest concentrations tested (e.g., decrease of fertilization success, delay of embryonic developmental stages or larval mortality). Exposure to low concentrations did not impact larval survival or development and even tended to enhance larval physiological state. Furthermore, no effects of marennine were observed on the fish gill cell line tested. Marennine could be viewed as a Jekyll and Hyde molecule, which possibly affects the earliest stages of development of some organisms but with no direct impacts on adults. Our results emphasize the need to determine dosages that optimize beneficial effects and critical concentrations not to be exceeded before considering the use of marennine in bivalve or fish hatcheries.

Toward Multiscale Modeling of Molecular and Biochemical Events Occurring at Fertilization Time in Sea Urchins.

We review here previous theoretical and experimental works, which aim to model major events that occur at the time of fertilization in the sea urchin. We discuss works that perform experiments and develop hypotheses that link different scales of biological systems such as the intracellular Ca2+ concentration oscillations and the swimming behavior of sperm, the Ca2+ wave propagation and the fertilization membrane elevation of the egg, and the mRNA translational activation and the completion of the first mitotic division of the early embryo. The aim of this review is on one hand, to highlight the value of systems biology for understanding the mechanisms associated with fertilization and early embryonic development in sea urchins. On the other hand, this review attempts to illustrate, for mathematicians and bioinformaticians, the potential that represent these molecular and cellular events for modeling clear physiological processes.

Translatome analysis at the egg-to-embryo transition in sea urchin.

Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis.