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We present the novel implementation of a non-differentiable metric approximation and a corresponding loss-scheduling aimed at the search for new particles of unknown mass in high energy physics experiments. We call the loss-scheduling, based on the minimisation of a figure-of-merit related function typical of particle physics, a Punzi-loss function, and the neural network that utilises this loss function a Punzi-net. We show that the Punzi-net outperforms standard multivariate analysis techniques and generalises well to mass hypotheses for which it was not trained. This is achieved by training a single classifier that provides a coherent and optimal classification of all signal hypotheses over the whole search space. Our result constitutes a complementary approach to fully differentiable analyses in particle physics. We implemented this work using PyTorch and provide users full access to a public repository containing all the codes and a training example.
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Cupriavidus pauculus is a Gram negative rod that is usually isolated in water and has been extraordinarily isolated in patients with vascular access and / or immunosuppression. In this work we present a series of cases of patients with positive blood cultures for cupríavidus pauculus, during a short period of time in our hospital center. The only characteristic related to all cases was the use of parenteral nutrition. It highlights the rareness of the microorganism and the susceptibility identified in the antibiogram, which may be useful for future cases.
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The aim of this study was to investigate the viability of Pediococcus acidilactici ATCC 8042 and Lactobacillus plantarum ATCC 8014 in a freeze-dried capsules system prepared with sodium alginate and gum arabic using the extrusion technique. The capsules made with alginate 2% (w/v)/gum arabic 2% (w/v) showed higher hardness (7.12 ± 0.71 N), with highly cohesive (0.81 ± 0.02) and elastic (0.99 ± 0.00) features on the Texture Profile Analysis (TPA), as well as higher sphericity with 1.75 ± 0.12 mm y 1.73 ± 0.13 mm diameter axes and regularity in their surface by Scanning Electron Microscopy (SEM). The use of skimmed milk at 10% as a cryoprotector in the freeze-drying process allowed the obtention of high viability percentages (88% a 96%) for both strains. Best results of viability for P. acidilactici encapsulated was with the use of alginate 2% (w/v)/gum arabic 2% (w/v) (92%±2.65), and L. plantarum with the use of alginate 2% (w/v) (84.71%±10.33) during the gastrointestinal environment challenge.
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Alginatos/química , Goma Arábiga/química , Lactobacillus plantarum/citología , Pediococcus acidilactici/citología , Encapsulación Celular , Células Inmovilizadas/citología , Liofilización , Tracto Gastrointestinal/microbiología , Humanos , Viabilidad Microbiana , Probióticos/análisisRESUMEN
This study evaluated the association between anorexia of aging and nutrients intake. It was a cross-sectional study with 130 individuals aged 60 years or older, undergoing outpatient care in the city of Campinas, São Paulo. Anorexia of Aging (AA) was assessed using the Simplified Nutrition Appetite Questionnaire (SNAQ), and food consumption was evaluated using the 24-hour recall (24HR). The prevalence of AA was 27.7%, in which 66.7% were women and 38.9% were older than 80 years. Elderly with AA presented lower intake of calories (1172.6 kcal vs 1477.9 kcal; p = 0.003), carbohydrates (158.5 g vs 194.1 g; p = 0.015), proteins (49.9 g vs 68.5 g; p = 0.004) and lipids (34.6 g vs 46.1 g; p = 0.006). They also had lower intake of fibers (12.6 g vs 19.4 g; p < 0.001), iron (6.4 mg vs 8.9 mg; p < 0.001) and zinc (6.0 mg vs 8.5 mg; p = 0.004). Our results show that intake of most nutrients is significantly lower in AA elderly, except carbohydrates, which may point to worse-quality diets. The diagnosis of AA, as well as the evaluation of elderly food intake, are essential to prevent undernutrition, vulnerabilities, and increased morbidity and mortality.
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Anorexia/diagnóstico , Dieta , Ingestión de Energía/fisiología , Estado Nutricional/fisiología , Anciano , Anciano de 80 o más Años , Envejecimiento , Apetito , Brasil , Estudios Transversales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Nutrientes/análisis , Encuestas y CuestionariosRESUMEN
INTRODUCTION: As the incidence of pediatric nephrolithiasis rises, understanding the efficacy and morbidity of surgical treatment options is critical. Currently, there are limited comparative data assessing shock wave lithotripsy (SWL) and ureteroscopy (URS) outcomes in children. OBJECTIVE: The objective of this study was to compare stone clearance, 30-day emergency department visits, and the number of general anesthetics required per stone treatment for both modalities. STUDY DESIGN: A multi-institutional retrospective review of children presenting for either URS or SWL between 2000 and 2017 was performed. Stone clearance, need for retreatment, the number of anesthetics, as well as the number and reason for emergency room visit were captured and compared between groups. Multivariate statistical analysis accounting for age, stone location, stone diameter, pre-intervention stent, and provider volume was performed for adjusted analysis. RESULTS: A total of 84 SWL and 175 URS procedures were included. Complete stone clearance and rates of residual stone fragments <4 mm after final procedure for SWL were 77.0% and 90.8% and for URS were 78.5% and 91.7%, respectively. Retreatment rates for both procedures were not significantly different (17.9% SWL vs. 18.9% URS, P = 0.85). Children who underwent SWL had lower rates of emergency room visits for infections (0% vs. 5.1%, P = 0.03) and flank pain (3.6% vs. 10.9%, P = 0.05) and required fewer general anesthetics per treatment (1.2 vs. 2.0, P < 0.01) than those who underwent URS (Figure). DISCUSSION: Stone clearance after both the initial and final treatments and need for repeat interventions were similar between surgical modalities. However, SWL carries less morbidity than URS. Specifically, patients who underwent SWL experienced lower rates of ED visits for urinary tract infection and for flank pain, parallel to conclusions in current comparative literature. In addition, SWL requires less general anesthetics (2.0 vs. 1.2), secondary to lower rates of ureteral stent placement and removal. Data on the potential risk of general anesthetics to neurodevelopment support thoughtful utilization of these medications. Limitations of this study include its retrospective nature and the prolonged 20-year time period over which data were collected. CONCLUSIONS: When adjusting for confounders, SWL and URS achieve similar stone clearance. In the setting of equivalent efficacy, considerations regarding necessity of repeat interventions, morbidity of anesthesia, and complications should be integrated into clinical practice.
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Cálculos Renales/terapia , Litotricia/métodos , Nefrolitiasis/terapia , Ureteroscopía/métodos , Cálculos Urinarios/terapia , Niño , Femenino , Estudios de Seguimiento , Humanos , Cálculos Renales/diagnóstico , Masculino , Nefrolitiasis/diagnóstico , Estudios Retrospectivos , Resultado del Tratamiento , Cálculos Urinarios/diagnósticoRESUMEN
BACKGROUND: Little is known about the effects of cocoa and its main flavanols on the prothrombotic state associated with the development of hypertension in diet-induced obesity models. PURPOSE: To evaluate the effects of cocoa powder, cocoa extract and their main flavanols on plasma biomarkers related to impaired coagulation and fibrinolysis and its association with hypertension and obesity-related metabolic disorders in rats fed a hypercaloric diet. METHODS: Male Wistar rats were randomly assigned to 7 treatment groups (n = 7): normal diet (ND); hypercaloric diet control group (HCD); HCD + cocoa powder (CO); HCD + cocoa extract (CO-EX); HCD + (-)-epicatechin (EPI); HCD + (+)-catechin (CAT); and HCD + procyanidin B2 (PB2). Blood pressure was measured using the tail-cuff method (week 7). At the end of the experimental period (week 8), rats were sacrificed and blood samples were collected immediately for coagulation and biochemical analyses. RESULTS: Oral administration of CO, CO-EX and their main flavanols significantly decreased plasma biomarkers related to impaired coagulation and fibrinolysis (vWF, FVIII, fibrinogen and PAI-1) in rats fed a hypercaloric diet. These effects were associated with decreased systolic and diastolic blood pressure, aortic oxidative stress (MDA levels) and improvement of dyslipidemia, insulin resistance and circulating markers of inflammation (TNF-α, IL-6 and CRP) compared to the HCD group. CONCLUSION: Our results showed that cocoa and its main flavanols may improve endothelial dysfunction and exert their antihypertensive effects by decreasing the prothrombotic state in rats fed a hypercaloric diet. Moreover, improvement of obesity-related metabolic disorders may also contribute to their BP-lowering effect.
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Chocolate/análisis , Flavonoles/farmacología , Hipertensión/inducido químicamente , Adipoquinas/metabolismo , Animales , Aorta , Biomarcadores , Flavonoles/química , Inflamación/metabolismo , Peroxidación de Lípido , Malondialdehído/metabolismo , Extractos Vegetales/química , Extractos Vegetales/farmacología , RatasRESUMEN
OBJECTIVE: to explore the relationship between anemia, hemoglobin concentration and frailty syndrome in older adults. RESEARCH METHODS AND PROCEDURES: This was a cross-sectional population-based study, with adults ≥60 years (n=1,256) from the third wave of the SABE Cohort Study (Health, Well-being and Aging) conducted in 2010 in São Paulo, Brazil. Frailty syndrome was evaluated according to Fried´s phenotype. Anemia was defined using the WHO criteria (hemoglobin concentration <12 g/dL for women and <13 g/dL for men). Four approaches were used to verify the associations between anemia, hemoglobin concentration and frailty status or number of frailty criteria. We used logistic regression and Poisson regression in the analyses, and they were adjusted in three hierarchical models using three blocks of variables: basic characteristics; clinical characteristics; cognitive status. RESULTS: Mean hemoglobin concentration was significantly lower in frail elderly (13.3g/dL, versus 14.3g/dL in non-frail; p<0.001). Prevalence of anemia was also significantly higher in frail when compared to non-frail elderly (24.2% and 3.8%; p<0.001). Anemia was significantly associated to low physical activity, weakness and slowness. In the fully adjusted regression models, anemia was strongly associated to frailty (OR=3.27, 95%IC=1.89,5.65; p<0.001), and lower levels of hemoglobin were associated to higher number of frailty criteria. CONCLUSIONS: We found important associations between anemia, hemoglobin concentration and frailty; anemic older adults were more likely to be frail, and lower levels of hemoglobin were associated to higher number of frailty criteria showing a clear dose-response effect.
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Envejecimiento , Anemia/epidemiología , Anciano Frágil , Hemoglobinas/metabolismo , Anciano , Anemia/sangre , Brasil/epidemiología , Estudios de Cohortes , Estudios Transversales , Femenino , Marcha , Humanos , Modelos Logísticos , Masculino , Actividad Motora , Debilidad Muscular , Prevalencia , SíndromeRESUMEN
OBJECTIVE: To examine the association between hemoglobin concentration and disability and mobility difficulty among older adults living in São Paulo, Brazil. DESIGN: Cross-sectional study. SETTING: Population-based study conducted in São Paulo, Brazil. PARTICIPANTS: Adults age 60 and over (n=1,256) from the third data collection wave of the SABE Study (Health, Well-being, and Aging) conducted in 2010. MEASUREMENTS: Two outcome measures were included in the analyses: 1) a difficulty to perform at least one of the instrumental activities of daily living (IADL) and 2) mobility difficulty, which was assessed using the Short Physical Performance Battery (SPPB). Logistic regression models assessed the association between hemoglobin and each of the outcome measures. All analyses were adjusted for sociodemographic and health characteristics. RESULTS: The prevalence of IADL disability was 26.8% and 10.7% of participants had mobility difficulty. The mean hemoglobin concentration was significantly lower in older adults who already presented disability (13.7g/dL versus 14.4g/dL among independent elderly) or decreased mobility (13.4g/dL versus 14.3g/dL among elderly with preserved mobility). Higher values of hemoglobin concentration were associated with lower the risk of IADL disability (OR=0.88; p=0.04) and mobility difficulty (OR=0.81; p=0.02). Hemoglobin concentrations showed a dose-response effect in the probability of each outcome. CONCLUSIONS: Lower hemoglobin concentration was associated with a higher probability of IADL disability and mobility difficulty, showing a clear dose-response effect.
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Personas con Discapacidad/estadística & datos numéricos , Hemoglobinas/análisis , Limitación de la Movilidad , Actividades Cotidianas , Anciano , Anciano de 80 o más Años , Envejecimiento/sangre , Envejecimiento/fisiología , Brasil/epidemiología , Estudios Transversales , Femenino , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , PrevalenciaRESUMEN
In this study, an immunoproteomic approach was used to identify immunodominant proteins from Mycoplasma mycoides subsp. capri isolates. Membrane proteins, extracted through TX-114 phase partitioning, were separated using mono- and two-dimensional electrophoresis and detected by Western blotting with pooled sera from naturally infected goats. A total of 27 immunoreactive spots, corresponding to 13 different proteins, were identified using nanoLC-ESI-MSMS. Function annotation revealed that most of these proteins were metabolic enzymes involved in carbohydrate and energy metabolism. The immunogenic proteins identified in this study: pyruvate dehydrogenase, dihydrolipoamide acetyltransferase, dihydrolipoyl dehydrogenase, phosphate acetyltransferase, phosphopyruvate hydratase, adenine phopshoribosyltransferase, transketolase, translation elongation factor G, translation elongation factor Ts, FMN-dependent NADH-azoreductase, peptide methionine sulfoxide reductase, inorganic diphosphatase and trigger factor may be used as biomarkers for the serological diagnosis of contagious agalactia caused by M. mycoides subsp. capri.
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Mycoplasma mycoides/inmunología , Proteómica , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Proteínas Bacterianas/aislamiento & purificación , Proteínas Bacterianas/metabolismo , Electroforesis en Gel de Poliacrilamida , Enzimas/genética , Enzimas/inmunología , Enzimas/aislamiento & purificación , Enzimas/metabolismo , Cabras , Sueros Inmunes/metabolismo , Immunoblotting , Mycoplasma mycoides/genética , Mycoplasma mycoides/metabolismo , Pleuroneumonía Contagiosa/diagnósticoRESUMEN
The primary reason for processing maize is to enhance feeding value. Total tract starch digestion is similar for coarsely processed (dry rolled, cracked) dry maize. Enhancements in starch digestion due to dry rolling maize v. feeding maize whole may be greater in light-weight calves than in yearlings, and when DM intake is restricted ( < 1·5 % of body weight). The net energy (NE) maintain (NEm) and NE gain (NEg) values for whole maize are 8·83 and 6·02 MJ (2·11 and 1·44 Mcal)/kg, respectively. Compared with conventional dry processing (i.e. coarse rolled, cracked), finely processing maize may increase the initial rate of digestion, but does not improve total tract starch digestion. Tempering before rolling (without the addition of steam) may enhance the growth performance response and the NE value of maize. Average total tract starch digestion is similar for high-moisture and steam-flaked maize. However, the proportion of starch digested ruminally is greater (about 8 %) for high-moisture maize. The growth performance response of feedlot cattle to the feeding of high-moisture maize is highly variable. Although the NEm and NEg value of whole high-moisture maize was slightly less than that of dry processed maize (averaging 9·04 and 6·44 MJ (2·16 and 1·54 Mcal)/kg, respectively), grinding or rolling high-moisture maize before ensiling increased (6 %) its NE value. Substituting steam-flaked maize for dry processed maize increases average daily gain (6·3 %) and decreases DM intake (5 %). The comparative NEm and NEg values for steam-flaked maize at optimal processing (density = 0·34 kg/l) are 10·04 and 7·07 MJ (2·40 and 1·69 Mcal)/kg, respectively. These NE values are greater (3 %) than current tabular values (National Research Council, 2000), being more consistent with earlier standards (National Research Council, 1984). When maize is the primary or sole source of starch in the diet, concentration of starch in faeces (faecal starch, % of DM) of feedlot steers can serve as an indicator of total tract starch digestion, and, hence, the feeding value of maize.
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Crianza de Animales Domésticos , Dieta/veterinaria , Digestión , Ingestión de Energía , Manipulación de Alimentos/métodos , Almidón/metabolismo , Zea mays , Animales , Metabolismo de los Hidratos de Carbono , Bovinos , Carbohidratos de la Dieta/metabolismo , Digestión/fisiología , Heces/química , Crecimiento , Valor Nutritivo , Rumen/metabolismoRESUMEN
Minimal encephalopathy was originally associated with chronic liver disease but is increasingly associated with most other chronic diseases and particularly with diabetes and also chronic disorders in other organs: kidneys, lungs, thyroid and with obesity. It is increasingly with dramatically increased and more or less permanent increase in systemic inflammation, most likely a result of Western lifestyle. Frequent physical exercise and intake of foods rich in vitamins, antioxidants, fibres, lactic acid bacteria etc in combination with reduction in intake of refined and processed foods is known to reduce systemic inflammation and prevent chronic diseases. Some lactic acid bacteria, especially Lb paracasei, lb plantarum and pediococcus pentosaceus have proven effective to reduce inflammation and eliminate encephalopathy. Significant reduction in blood ammonia levels and endotoxin levels were reported in parallel to improvement of liver disease. Subsequent studies with other lactic acid bacteria seem to demonstrate suppression of inflammation and one study also provides evidence of clinical improvement.
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Encefalopatías Metabólicas/prevención & control , Inflamación/prevención & control , Cirrosis Hepática/prevención & control , Prebióticos , Probióticos/uso terapéutico , Encefalopatías Metabólicas/etiología , Enfermedad Crónica , Proteínas en la Dieta/efectos adversos , Enfermedad Hepática en Estado Terminal/complicaciones , Enfermedad Hepática en Estado Terminal/prevención & control , Hipersensibilidad a los Alimentos/complicaciones , Tracto Gastrointestinal/inmunología , Encefalopatía Hepática/etiología , Encefalopatía Hepática/prevención & control , Humanos , Inflamación/etiología , Estilo de VidaRESUMEN
Four Holstein steers (137 ± 2 kg) with cannulas in the rumen and proximal duodenum were used in a 4 × 4 Latin square design to evaluate the influence of processing method on comparative digestion of white corn. Treatments consisted of a basal finishing diet containing 80% corn grain (DM basis) as 1) dry-rolled white corn (DRWC), 2) steam-flaked white corn (SFWC) with 0.36 kg/L flake density (SFWC36), 3) SFWC, 0.31 kg/L flake density (SFWC31), and 4) steam-flaked yellow corn (SFYC) with 0.31 kg/L flake density (SFYC31). Characteristics of ruminal, postruminal, and apparent total tract digestion of OM, starch, and N were similar (P ≥ 0.08) for SFYC31 and SFWC31 treatments. Decreasing flake density of white corn (from 0.36 to 0.31 kg/L) did not affect (P = 0.22) ruminal OM digestion, but increased (1.9%, P = 0.07) apparent total tract OM digestion. Compared with dry rolling, steam flaking white corn increased ruminal (9.4%, P = 0.05), postruminal (14.4%, P < 0.01), and apparent total tract OM digestion (8.2%, P < 0.01), reflecting corresponding increases in ruminal (13.3%, P < 0.01), postruminal (43%, P < 0.01), and apparent total tract (12.3%, P < 0.01) starch digestion. Apparent postruminal and apparent total-tract N digestion also were greater (6.5 and 5.6%, respectively, P = 0.04) for SFWC than for DRWC. The DE value of SFWC and SFYC diets was similar, averaging 3.39 Mcal/kg. The DE value of SFWC was greater (8.1%, P < 0.01) than that of DRWC. Ruminal pH (4 h postprandial) averaged 5.74 and was not affected (P ≥ 0.48) by dietary treatments. Compared with dry rolling, steam flaking markedly enhances the feeding value of white corn, with optimal flake density being less than 0.36 kg/L. Although white corn has greater vitreous endosperm content, characteristics of ruminal starch digestion and undegradable intake protein are similar to conventional yellow dent corn when processed to a similar flake density (0.31 kg/L). However, postruminal and apparent total tract starch digestion tends to be slightly less for flaked white corn than for yellow corn.
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Alimentación Animal/análisis , Dieta/veterinaria , Digestión/fisiología , Manipulación de Alimentos/métodos , Zea mays/clasificación , Crianza de Animales Domésticos , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Bovinos , Masculino , Valor Nutritivo , VaporRESUMEN
Adsorption isotherms of chlorobenzene, chloroform and carbon tetrachloride vapors on undoped SiO(2), and metal-doped Ag/SiO(2), Cu/SiO(2) and Fe/SiO(2) substrates were measured in the temperature range of 398-593K. These substrates were prepared from a typical sol-gel technique in the presence of metal dopants that rendered an assortment of microporous-mesoporous solids. The relevant characteristic of these materials was the different porosities and micropore to mesopore volume ratios that were displayed; this was due to the effect that the cationic metal valence exerts on the size of the sol-gel globules that compose the porous solid. The texture of these SiO(2) materials was analyzed by X-ray diffraction (XRD), FTIR, and diverse adsorption methods. The pore-size distributions of the adsorbents confirmed the existence of mesopores and supermicropores, while ultramicropores were absent. The Freundlich adsorption model approximately fitted the chlorinated compounds adsorption data on the silica substrates by reason of a heterogeneous energy distribution of adsorption sites. The intensity of the interaction between these organic vapors and the surface of the SiO(2) samples was analyzed through evaluation of the isosteric heat of adsorption and standard adsorption energy; from these last results it was evident that the presence of metal species within the silica structure greatly affected the values of both the amounts adsorbed as well as of the isosteric heats of adsorption.
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Tetracloruro de Carbono/química , Clorobencenos/química , Cloroformo/química , Metales/química , Adsorción , Algoritmos , Cromatografía de Gases , Cobre/química , Geles , Hierro/química , Nitrógeno/química , Porosidad , Dióxido de Silicio/química , Plata/química , Espectroscopía Infrarroja por Transformada de Fourier , Termodinámica , Difracción de Rayos XRESUMEN
To evaluate the utility of N as a digestion marker to predict total tract starch digestion, data from 32 metabolism trials involving 147 steers and 637 individual starch digestibility measurements were compiled. All trials were conducted at the University of California Desert Research and Extension Center. Total tract starch digestibility was determined from concentrations of starch and chromic oxide in feed and feces. In all trials, the steers were adapted to diets for 10 d followed by 4 d for collection of samples of feces. During collection, fecal samples (approximately 200 g, wet basis) were obtained twice daily. Samples from each steer within each collection period were composited for analysis. Diets contained 46.5 +/- 7.4% starch and 1.85 +/- 0.20% N. Apparently digestible N as a percentage of diet DM was closely associated (r(2) = 0.73; P < 0.001) with dietary N concentration. Fecal N concentration (FN, % of DM) explained 35% of the variation in fecal DM excretion (S(y.x) = 4.3; P < 0.001). Incorporating FN into the model, starch digestion was estimated as follows: starch digestion, % of intake = 100 {1 - [(0.938 -0.497FN + 0.0853FN(2)) FS/DS]}, where FS is fecal starch concentration (% of DM) and DS is dietary starch concentration (% of DM; r(2) = 0.94; S(y.x) = 0.68; P < 0.001). Fecal starch concentration alone explained 96% of the variation (S(y.x) = 0.45; P < 0.001) in total tract starch digestion: starch digestion, % = 99.9 - 0.413FS -0.0104FS(2). Omitting cases in our data set in which observed total tract starch digestion was less than 95%, the r(2) between FS and starch digestibility decreased to 0.82 (S(y.x) = 0.26; n = 529). However, estimated starch digestion using the equation incorporating FN remained closely associated with the observed starch digestion (r(2) = 0.90; S(y.x) = 0.22; P < 0.001; n = 529). Equations also were developed to predict NE(m) and NE(g) concentrations of common feed grains based on starch digestibility and FS. Starch digestion can be accurately predicted based on FS. However, incorporation of FN into the model markedly enhanced the estimates of grain quality and the efficacy of processing when total tract starch digestion exceeded 95%.
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Alimentación Animal/análisis , Bovinos/metabolismo , Digestión , Heces/química , Nitrógeno/metabolismo , Almidón/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Digestión/efectos de los fármacos , Digestión/fisiología , Masculino , Nitrógeno/análisis , Valor Predictivo de las Pruebas , Distribución Aleatoria , Almidón/análisisRESUMEN
Eight cannulated Holstein steers (average BW: 251 kg) were used in 2 simultaneous 4 x 4 Latin squares in a split-plot arrangement to test the effects of processing method [dry-rolled (DR) vs. steam-flaked (SF); main plot] and vitreousness (V, %; subplot) of yellow dent corn (V55, V61, V63, and V65) on site of digestion of diets containing 73.2% corn grain. No vitreousness x processing method interactions were detected for ruminal digestion, but ruminal starch digestion was 14.4% lower (P < 0.01) for DR than for SF corn. Interactions were detected between vitreousness and processing method for postruminal (P < 0.10) and total tract digestion (P < 0.05). With DR, vitreousness tended to decrease (linear effect, P < 0.10) postruminal OM and starch digestion. With SF, vitreousness did not affect (P > or = 0.15) postruminal digestion of OM and starch. Postruminal N digestion tended to decrease (linear effect, P = 0.12) as vitreousness increased. Postruminal digestion was greater for SF than for DR corn OM (25.7%, P < 0.05), starch (94.3%, P < 0.10), and N (10.7%, P < 0.01). Steam flaking increased total tract digestion of OM (11%, P < 0.05), starch (16%, P < 0.01), and N (8.4%, P < 0.05) but decreased total tract ADF digestion (26.7%, P < 0.01). With DR, total tract starch digestion was lower for V65 (cubic effect, P < 0.10) than for the other hybrids. With SF, total tract starch digestion was not affected (P > or = 0.15) by vitreousness. Fecal starch and total tract starch digestion were inversely related (starch digestion, % = 101 - 0.65 x fecal starch, %; r2 = 0.94, P < 0.01). Ruminal pH was greater for steers fed DR than for steers fed SF corn (6.03 vs. 5.62, P < 0.05). Steam flaking decreased (P < 0.01) the ruminal molar proportion of acetate (24%), acetate:propionate molar ratio (55%), estimated methane production (37.5%), and butyrate (11.3%, P < 0.05). There was a vitreousness x processing interaction (P < 0.01) for acetate:propionate. For DR, acetate:propionate tended to increase (linear effect; P < 0.10) with increasing vitreousness. With SF, acetate:propionate was greater (cubic effect, P < 0.01) for V65. Starch from more vitreous corn grain was less digested when corn grain was DR, but this adverse effect of vitreousness on digestion was negated when the corn grain was SF. Of the 19% advantage in energetic efficiency associated with flaked over rolled corn grain, about 3/4 can be attributed to increased OM digestibility, with the remaining 1/4 ascribed to reduced methane loss.
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Alimentación Animal , Bovinos/metabolismo , Dieta/veterinaria , Digestión/fisiología , Zea mays/química , Zea mays/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Femenino , Zea mays/genéticaRESUMEN
The membrane-protein 81 gene (mb-mp81) of Mycoplasma bovis was cloned, sequenced and compared to membrane-protein 81 gene (ma-mp81) of Mycoplasma agalactiae. After alignment of both sequences, specific primers pairs were designed from variable or unchanging nucleotide segments. In this study, we describe the development and optimization of a multiplex-PCR (MPCR) for the rapid detection of M. agalactiae and M. bovis strains. In addition, a simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the restriction enzymes AluI, DraI, RsaI and XbaI, is described to distinguish between both species. The results suggest that MPCR and PCR-RFLP assays could be used as an alternative method in routine diagnosis for rapid and specific simultaneous detection of M. agalactiae and M. bovis.
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Mycoplasma agalactiae/genética , Mycoplasma agalactiae/aislamiento & purificación , Mycoplasma bovis/genética , Mycoplasma bovis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo de Longitud del Fragmento de Restricción , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Clonación Molecular , Diagnóstico Diferencial , Proteínas de la Membrana/química , Proteínas de la Membrana/genética , Peso MolecularRESUMEN
Polyethyleneimine (PEI)-coated slides were used for attachment of unknown extremophiles and their cultures grown at high temperature and low pH. Selective adhesion of cells in natural samples and cultural isolates was compared to polylysine (PL) and several other coatings. PEI is superior to PL for adhesion of extremophiles.
Asunto(s)
Bacterias/citología , Microscopía Electrónica de Rastreo/métodos , Polietileneimina/química , Sulfolobus/citología , Adherencias TisularesRESUMEN
In affluent societies high caloric intake and chronic stress are currently associated with upper body fat. We investigated the effects of a high-sucrose (S) diet and dexamethasone (DEX) on fat depots (experiment 1) and lipid fuel fluxes (experiment 2) in male Wistar rats. In experiment 1, a liquid diet of commercial powdered milk containing 31% calories as carbohydrate or an isocaloric S diet (80% calories as carbohydrate) was offered to male rats. One half of the rats on each diet received a daily dose of 3 microg DEX in their diet. Intake was measured daily and body weight 3 times a week. Rats were killed after 7 weeks, and fat depot weights and carcass lipid were determined. In a second experiment, other rats received only the S diet with or without DEX. After 7 weeks, under pentobarbital anesthesia, arterial, portal, and iliolumbar vein blood was drawn, and the liver was extracted. Plasma concentration of triacylglycerides (TAG), nonesterified fatty acids (NEFA), glycerol (GOL), and lactate (L) and liver TAG were measured. Rats on the S diet ingested less and gained less weight. DEX treatment significantly reduced body weight gain. All fat depots as percentage of body weight were increased only in the S-DEX group. The S-DEX group had more liver TAG and less arterial NEFA and GOL than the S group. TAG determinations showed unexpected results: portal levels in the S-DEX group and iliolumbar levels in both groups were significantly higher than in the arterial plasma. This fact, together with high NEFA/GOL ratios in these veins, may signify incomplete TAG hydrolysis by lipoprotein lipase. L levels were higher in the S-DEX group and higher in arterial versus venous blood in both groups, indicating L uptake both in the splanchnic area and the retroperitoneal fat. These results show that, in rats, a long-term high-sucrose diet has peculiar effects on L turnover, and when associated with DEX, it also increases fat depots, induces liver steatosis, and, presumably, inhibits complete hydrolysis of TAG by lipoprotein lipase (LPL).
Asunto(s)
Tejido Adiposo/metabolismo , Dexametasona/farmacología , Sacarosa en la Dieta/administración & dosificación , Metabolismo de los Lípidos , Hígado/metabolismo , Mesenterio/metabolismo , Animales , Glicerol/metabolismo , Lipólisis , Masculino , Ratas , Ratas Wistar , Espacio RetroperitonealRESUMEN
It is known that lactation induces a mild hypothyroid state in rats and other mammals while thyroid hormone administration increases milk secretion in ruminants. The aim of this study was to investigate the effects of a moderate dose of 3,5,3'-triiodothyronine (T3), administered to rat dams during lactation on pups' growth and milk yield and composition. Primiparous Wistar rats with litters adjusted to 10 pups per dam received either tap water or T3 (75 microg/kg x day) in their drinking water from parturition till weaning. Food and water intake of dams and body weight of dams and pups were measured daily. In other groups of rats with similar treatments, milk yield of dams, macronutrient milk composition, and mammary arteriovenous differences for triglycerides (TG) and glucose were also determined. Dams treated with T3 ingested more food and their pups gained more weight than controls. Milk yield, milk TG concentration and glucose extraction by mammary glands were also higher in T3 treated dams. The results show that compensation of the mild hypothyroidism of the lactating rat may contribute to an increase in milk production and lipid levels, leading to an increase in growth of pups.
Asunto(s)
Animales Recién Nacidos/crecimiento & desarrollo , Lactancia/efectos de los fármacos , Leche/efectos de los fármacos , Triglicéridos/metabolismo , Triyodotironina/farmacología , Animales , Peso Corporal/efectos de los fármacos , Femenino , Leche/fisiología , Ratas , Ratas Wistar , Triyodotironina/administración & dosificaciónRESUMEN
The effects of 3,5,3'-triiodothyronine (T3) levels on threshold, latency and duration of pentylenetetrazole-induced seizures were tested in rats treated with thyroxine (300 micrograms/kg.day, N = 9) or methimazole (60 mg/kg.day, N = 5) dissolved in drinking water. Compared to controls (N = 7), methimazole treatment reduced T3 levels (45.4 +/- 2.0 vs. 33.0 +/- 4.8 ng/dl) and increased seizure duration (36.2 +/- 22.4 vs. 289.6 +/- 24.4 s) and threshold (29.0 +/- vs. 45.5 mg/kg). Thyroxine treatment increased T3 levels (45.4 +/- 2.0 vs. 67.7 +/- 4.8 ng/dl), but had no significant effect on seizures.