PP2A and GSK3 act as modifiers of FUS-ALS by modulating mitochondrial transport.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease which currently lacks effective treatments. Mutations in the RNA-binding protein FUS are a common cause of familial ALS, accounting for around 4% of the cases. Understanding the mechanisms by which mutant FUS becomes toxic to neurons can provide insight into the pathogenesis of both familial and sporadic ALS. We have previously observed that overexpression of wild-type or ALS-mutant FUS in Drosophila motor neurons is toxic, which allowed us to screen for novel genetic modifiers of the disease. Using a genome-wide screening approach, we identified Protein Phosphatase 2A (PP2A) and Glycogen Synthase Kinase 3 (GSK3) as novel modifiers of FUS-ALS. Loss of function or pharmacological inhibition of either protein rescued FUS-associated lethality in Drosophila. Consistent with a conserved role in disease pathogenesis, pharmacological inhibition of both proteins rescued disease-relevant phenotypes, including mitochondrial trafficking defects and neuromuscular junction failure, in patient iPSC-derived spinal motor neurons (iPSC-sMNs). In FUS-ALS flies, mice, and human iPSC-sMNs, we observed reduced GSK3 inhibitory phosphorylation, suggesting that FUS dysfunction results in GSK3 hyperactivity. Furthermore, we found that PP2A acts upstream of GSK3, affecting its inhibitory phosphorylation. GSK3 has previously been linked to kinesin-1 hyperphosphorylation. We observed this in both flies and iPSC-sMNs, and we rescued this hyperphosphorylation by inhibiting GSK3 or PP2A. Moreover, increasing the level of kinesin-1 expression in our Drosophila model strongly rescued toxicity, confirming the relevance of kinesin-1 hyperphosphorylation. Our data provide in vivo evidence that PP2A and GSK3 are disease modifiers, and reveal an unexplored mechanistic link between PP2A, GSK3, and kinesin-1, that may be central to the pathogenesis of FUS-ALS and sporadic forms of the disease.

Analysis of temperature effect in the CO2 absorption using a deep eutectic solvent: An in silico approach.

The excess level of carbon dioxide in the atmosphere has contributed a lot to global warming, occasioning several damages to the planet. Therefore, it is urgent to find ways to capture this gas. Then, the present work analyzed the temperature effect in CO2 absorption through deep eutectic solvents (DESs) based on urea and choline chloride using an in silico approach. The Molecular Dynamics (MD) simulations indicated that the increased temperature reduced the interaction potential of carbon dioxide molecules with the DESs components, indicating that the absorption process is more favorable at 303 K. On the other hand, the Noncovalent Interactions (NCI) simulations suggest that the increased temperature reduced the strong attractions and increased repulsive interactions between the carbon dioxide molecules with the solvent analyzed. Therefore, both in silico approaches suggest that the carbon dioxide absorption is more indicated at 303 K.

Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis.

Cancers arise through the accumulation of genetic and epigenetic alterations that enable cells to evade telomere-based proliferative barriers and achieve immortality. One such barrier is replicative crisis-an autophagy-dependent program that eliminates checkpoint-deficient cells with unstable telomeres and other cancer-relevant chromosomal aberrations1,2. However, little is known about the molecular events that regulate the onset of this important tumour-suppressive barrier. Here we identified the innate immune sensor Z-DNA binding protein 1 (ZBP1) as a regulator of the crisis program. A crisis-associated isoform of ZBP1 is induced by the cGAS-STING DNA-sensing pathway, but reaches full activation only when associated with telomeric-repeat-containing RNA (TERRA) transcripts that are synthesized from dysfunctional telomeres. TERRA-bound ZBP1 oligomerizes into filaments on the outer mitochondrial membrane of a subset of mitochondria, where it activates the innate immune adapter protein mitochondrial antiviral-signalling protein (MAVS). We propose that these oligomerization properties of ZBP1 serve as a signal amplification mechanism, where few TERRA-ZBP1 interactions are sufficient to launch a detrimental MAVS-dependent interferon response. Our study reveals a mechanism for telomere-mediated tumour suppression, whereby dysfunctional telomeres activate innate immune responses through mitochondrial TERRA-ZBP1 complexes to eliminate cells destined for neoplastic transformation.

(Bio)Sensing Strategies Based on Ionic Liquid-Functionalized Carbon Nanocomposites for Pharmaceuticals: Towards Greener Electrochemical Tools.

The interaction of carbon-based nanomaterials and ionic liquids (ILs) has been thoroughly exploited for diverse electroanalytical solutions since the first report in 2003. This combination, either through covalent or non-covalent functionalization, takes advantage of the unique characteristics inherent to each material, resulting in synergistic effects that are conferred to the electrochemical (bio)sensing system. From one side, carbon nanomaterials offer miniaturization capacity with enhanced electron transfer rates at a reduced cost, whereas from the other side, ILs contribute as ecological dispersing media for the nanostructures, improving conductivity and biocompatibility. The present review focuses on the use of this interesting type of nanocomposites for the development of (bio)sensors specifically for pharmaceutical detection, with emphasis on the analytical (bio)sensing features. The literature search displayed the conjugation of more than 20 different ILs and several carbon nanomaterials (MWCNT, SWCNT, graphene, carbon nanofibers, fullerene, and carbon quantum dots, among others) that were applied for a large set (about 60) of pharmaceutical compounds. This great variability causes a straightforward comparison between sensors to be a challenging task. Undoubtedly, electrochemical sensors based on the conjugation of carbon nanomaterials with ILs can potentially be established as sustainable analytical tools and viable alternatives to more traditional methods, especially concerning in situ environmental analysis.

Electrochemical and theoretical investigation on the behavior of the Co2+ ion in three eutectic solvents.

Deep eutectic solvents (DESs) have many advantages, making them a promising alternative in replacing ionic liquids and organic solvents. Besides, DESs have received much prominence due to their diverse applications: Electrodeposition of metals, organic synthesis, gas adsorption, and biodiesel production. Therefore, this work analyzed the effect of the temperature increase (298 K-353 K) on the behavior of the Co2+ ions in three eutectic solvents through electrochemical techniques and computational simulations. From the electrochemical analysis realized, the increase in temperature caused a reduction in specific mass and an increase in the diffusion coefficient. Besides, the activation energy values were of 15.3, 29.9, and 55.2 kJ mol-1 for 1ChCl:2 EG, 1ChCl:2U, and 1ChCl:2G, respectively. The computational simulations indicate that the increased temperature effect caused the replacement of HBD molecules by anions chloride around Co2+ ions for the SDW1 and SDW3 systems between the temperatures of 298 K-353 K, except for the SDW2 system that the replaced occurred in the interval of 313 K-353 K. Besides, the increase of temperature occasioned the increase of strength for Co-Cl interaction and weakened the interactions between the Co2+ ions with the oxygen of HBD molecules.

Corpos jovens em evidência no contexto da pandemia: o que pode um corpo? / Young bodies in the spotlight during the pandemic: what can a body do? / Cuerpos jóvenes en evidencia en el contexto de la pandemia: ¿qué puede hacer un cuerpo?

RESUMO Neste artigo discutimos as experiências de um projeto de ensino/extensão, com foco em reflexões acerca do corpo no ambiente escolar. Apresentamos um relato de oficinas realizadas entre o final de 2019 e o primeiro ano da pandemia, com uma turma do ensino médio de um colégio de aplicação, vinculado a uma universidade pública do Rio de Janeiro. O fio condutor partiu da pergunta "O que pode um corpo?", buscando problematizar como esses/as estudantes vinham constituindo os processos de "ser corpo" e como lidavam com isso na vida escolar. O projeto se orientava a cada encontro pelas narrativas discentes, provocando formas outras de pensar e sentir o corpo e produzindo, coletivamente, possibilidades de ampliar os modos de ver e sentir os corpossujeitos.

ABSTRACT This article discusses the experiences of a teaching and extension project, focusing on reflections of the body in the school environment. It presents the report of workshops held between the end of 2019 and the first year of the pandemic, in 2020, with a high school class from a College of Application, linked to a public university. The guiding thread started from the question "What can a body do?", with the aim of how these students were constituting the processes of "being a body" and how they dealt with it in school life. . The work was guided by the narratives of these students, provoking other ways of thinking and feeling the body and collectively producing possibilities to expand the ways of seeing and feeling the body-subjects.

RESUMEN Este artículo discute las experiencias de un proyecto de enseñanza y extensión, que se centró en reflexiones sobre el cuerpo en el ámbito escolar. Presenta el informe de talleres realizados entre finales de 2019 y el primer año de la pandemia, en 2020, con una clase de bachillerato de una Facultad de Aplicación, vinculada a una universidad pública. El hilo conductor partió de la pregunta "¿Qué puede hacer un cuerpo?", con el objetivo de cuestionar cómo estos estudiantes estaban constituyendo los procesos de "ser cuerpo" y cómo lo enfrentaban en la vida escolar. El trabajo fue guiado por las narrativas de estos estudiantes. Así, buscamos provocar otras formas de pensar y sentir el cuerpo, para producir colectivamente posibilidades de ampliar las formas de ver y sentir los cuerpos vivos y palpitantes.

Orthodontic Records Helping to Identify A Victim of Execution

ABSTRACT: Human identification is the process that identifies an individual as a unique being, differentiating him/ her from all others. The participation of Odontology in the processes of identification is fundamental in situations of human remains. This paper aims to report a case of positive identification, through dentistry, in human remains. The forensic odontologist performed a post-morten (PM) examination, which consisted of the collection of post-mortem dental findings in human bones, collection of ante-mortem (AM) dental findings in the patient's orthodontic records, and the comparison between AM and PM information. Dental elements are widely used in the processes of human identification, since they are resistant to time and to physical, chemical and mechanical impacts. Positive identification is guided by the presence of individualizing characteristics, easily detected in dental records. It is concluded that human identification through dentistry is a safe and reliable method, since dental elements have individualizing characteristics that make the process possible.

RESUMEN: La identificación humana es el proceso que identifica a un individuo como un ser único, diferenciándolo de todos los demás. La participación de la Odontología en los procesos de identificación es fundamental en situaciones de restos humanos. Este trabajo tiene como objetivo reportar un caso de identificación positiva, a través de la odontología, en restos humanos. El odontólogo forense realizó un examen post-morten (PM), que consistió en la recolección de hallazgos dentales post-mortem en huesos humanos, recolección de hallazgos dentales ante-mortem (AM) en los registros de ortodoncia del paciente y la comparación entre AM y Información de PM. Los elementos dentales son ampliamente utilizados en los procesos de identificación humana, ya que son resistentes al tiempo y a los impactos físicos, químicos y mecánicos. La identificación positiva está guiada por la presencia de características individualizantes, que se detectan fácilmente en los registros dentales. Se concluye que la identificación humana a través de la odontología es un método seguro y confiable, ya que los elementos dentales tienen características individualizantes que hacen posible el proceso.

Electrochemical sensor based on multi-walled carbon nanotubes for imidacloprid determination.

A simple and robust sensor (fMWCNT-Nafion®0.5%/GCE) for determination of imidacloprid (IMI), a widely used neonicotinoid, was developed using a glassy carbon electrode (GCE) modified with functionalized multi-walled carbon nanotubes (fMWCNT) and Nafion®. The obtained data suggest that IMI reduction is an irreversible process, due to the reduction of the nitro group to hydroxylamine derivatives, with the participation of two protons and four electrons, and a charge transfer coefficient of 0.141. The optimized square-wave voltammetric conditions were: McIlvaine buffer at pH 6.0, 0.5% of Nafion® in the fMWCNT suspension, -0.6 V and 180 s as accumulation potential and time, respectively. A linearity in the range of 2.00 × 10-7 to 1.77 × 10-6 mol L-1 IMI, with the values of limit of detection and limit of quantification were equal to 3.74 × 10-8 mol L-1 and 1.25 × 10-7 mol L-1, respectively. Repeatability and reproducibility displayed relative standard deviations lower than 5%. Recovery tests performed in tap water, melon, and shrimp yielded mean values of 94 ± 6%, 97 ± 10% and 93 ± 10%, respectively. Moreover, several inorganic and organic compounds did not significantly interfere (0.6 to 4.5%) on the IMI signal, proving the selectivity and applicability of the developed sensor for IMI detection in complex samples.

Molecular approach about the effect of water on the electrochemical behaviour of Ag+ ions in urea-choline chloride-water mixture.

The water influence on electrochemical behaviour of Ag+ ions in urea and choline chloride mixture was investigated by cyclic voltammetry technique, while the molecular insights about the investigated systems were obtained from molecular dynamic (MD) simulation. The water content was variated from 0 up to 10% (v/v). Cyclic voltammetry technique showed that the peak potential for Ag+/Ag redox couples shifted in direction to more positive potentials with the gradual increase of water content in solution, indicating that the addition of water electrocatalyses the kinetics of the reduction of Ag+ ions. The MD simulations demonstrated that water molecules do not interact strongly with Ag+ ions but induce a small reduction in the number of urea molecules around of the ion and that the water molecules adjust to free spaces in the mixture.

Performance and comparison of the London Atlas technique and Cameriere's third molar maturity index (I3M) for allocating individuals below or above the threshold of 18 years.

Age estimation is an important procedure in the forensic practice, especially when it comes to the criminal imputability of juvenile offenders. This study aimed to compare two radiographic methods for dental age estimation in a population of subadults from Northeast Brazil considering their performance on allocating individuals below or above the age threshold of 18 years. A cross-sectional observational study was designed. The sample consisted of 1200 panoramic radiographs of Northeastern Brazilian females (n = 600) and males (n = 600) aged between 16 and 21 years. Dental age estimation was performed using the London Atas technique and Cameriere's third molar maturity index (I3M). Statistical tests were performed to assess the sensitivity, specificity and accuracy of the two methods on distinguishing individuals below or above the legal age threshold of 18 years. Considering the total sample, the London Atlas technique reached sensitivity of 92.3%, specificity of 56% and accuracy of 79.9%. I3M revealed sensitivity of 94.1%, specificity of 55.4% and accuracy of 79.8%. Specificity rates systematically decreased when the sample was progressively stratified based on age groups that narrowed close to 18 years. The methods performed very similar (0.878). The combination of methods did not improve the performance on dental age estimation. The London Atlas and the I3M methods similarly distinguished Brazilian individuals as minors or not. Their performance, however, was suboptimal because of the low rates of specificity that could negatively influence on Court decisions. In other words, the methods could wrongly classify minors into the age of legal majority (culminating in false positives). In practice, the misleading classification could restrict individual rights (in the civil scenario) or even support more severe penalties (in the criminal scenario).

Maxillofacial and dental-related injuries from a Brazilian forensic science institute: Victims and perpetrators characteristics and associated risk factors.

BACKGROUND: Trauma due to external causes represents one of the greatest challenges for public health services in different regions around the world. This study aimed to determine the prevalence of facial trauma, associated risk factors, and classification of body injuries in individuals who underwent forensic examination in a Brazilian center. MATERIAL AND METHODS: Data were collected at the Ceará State Forensic Medicine unit in a 12-year period. Sociodemographic data related to the etiological agent and lesions resulting from the bodily injury were recorded. RESULTS: Among 1,031 physical injury exams, physical aggression (p<0.001), male victims aged between 21 and 30 years (p<0.001), salaried workers (p<0.001), and soft tissue and dentoalveolar injuries were significant findings. Regarding aggression, domestic violence was prevalent (p<0.001), perpetrated by the victim's partner (p<0.001), using a blunt instrument during the aggression (p<0.001), and directly associated with soft tissue injury (p<0.001). In traffic accidents, the most common type was motorcycle accident (p<0.001), on weekdays (p=0.036), at nighttime (p=0.134), showing a significant association with bone fractures (p=0.001). CONCLUSIONS: Oral and maxillofacial injuries obtained from a Brazilian forensic science center were significantly associated with sociodemographic and etiological factors. Key words:Forensic dentistry, facial trauma, violence, public health.

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review.

Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. However, most importantly, their relatively good conductivity enabling appropriate electron transfer rates-as well as their high surface area yielding attachment and extraordinary loading capacity for biomolecules-have been relevant and desirable features, justifying the key role that they have been playing, and will continue to play, in electrochemical (bio)sensor development. The present review outlines the contribution of carbon nanomaterials (carbon nanotubes, graphene, fullerene, carbon nanofibers, carbon black, carbon nanopowder, biochar nanoparticles, and graphite oxide), used alone or combined with other (nano)materials, to the field of environmental (bio)sensing, and more specifically, to pharmaceutical pollutants analysis in waters and aquatic species. The main trends of this field of research are also addressed.

Análise temporal das agressões físicas contra a mulher sob a perspectiva da odontologia legal na cidade de Fortaleza, Ceará / Temporal analysis of physical aggressions against women under the perspective of Legal Dentistry in the city of Fortaleza, Ceará

Introdução: A região de cabeça e pescoço é um dos sítios mais atingidos em casos de violência contra a mulher, fato que torna o odontolegista fundamental nas perícias de lesão corporal. O objetivo desse trabalho foi realizar uma análise temporal dos casos de agressão física contra as mulheres periciadas no serviço de Odontologia Forense da Perícia Forense do Estado do Ceará (PEFOCE) entre 2002 e 2017 e avaliar o impacto da lei Maria da Penha sobre as agressões físicas na região bucomaxilofacial. Metodologia: Tratou-se de um estudo quantitativo, longitudinal, do tipo série temporal. Foram coletados dados secundários, através dos laudos forenses emitidos pelos peritos odontolegistas da PEFOCE sede Fortaleza- CE no período de 2002 a 2017. Utilizou-se como ponto de corte para análise comparativa o ano de publicação da lei Maria da Penha. Resultados: Observou-se que a faixa etária de 30 e 59 anos foi a mais acometida e o (ex)companheiro o principal agressor. O local de maior ocorrência das agressões foi a via pública. Em 42% dos casos foram registradas agressões de gravidade leve, 86,3% dos laudos apontaram lesões por instrumento contundente e em 31% o tecido atingido foi apenas tecido mole. Observou-se uma mudança no perfil das agressões e um aumento das denuncias após a Lei Maria da Penha, que passaram a ser realizadas por mulheres mais velhas e apresentar menor gravidade das lesões. Conclusão: Dessa forma, sugere-se o impacto efetivo da Lei Maria da Penha, ao reduzir a gravidade das lesões localizadas na região bucomaxilofacial

Introduction: The head and neck region is one of the most affected sites in cases of violence against women, which makes the forensic dentistry essential in the field of bodily injuries. The objective of this study was to carry out a temporal analysis of the physical aggression cases against women who were interviewed at the Forensic Service of the State of Ceará, Brasil between 2002-2017 and to evaluate the impact of the 'Maria da Penha' law on physical aggression in the bucomaxillofacial region. Methods: This is a longitudinal, quantitative, time-series study. Secondary data were collected, through the forensic reports issued by the forensic dentistry service in the period from 2002-2017. The year of publication of the "Maria da Penha" law was used as a cut-off point for comparative analysis. Results: It was observed that the age group of 30-59 years old was the most affected and the (ex)partner the main aggressor. The most frequent place of aggression was the public road. 42% of the cases, aggressions of mild severity were recorded, 86.3% of the reports indicated lesions by forceful instrument and in 31% the tissue reached was only soft tissue. There was a change in the profile of the aggressions and an increase in complaints after the "Maria da Penha" Law, which were carried out by older women and presented less severity of the injuries. Conclusion: Thus, we suggest the effective impact of the "Maria da Penha" Law, by reducing the severity of the lesions located in the bucomaxillofacial region

Importância do registro das ausências dentais para a identificação humana: relato de caso / Importance of the registration of dental absences for human identification: case report

Identificar é determinar ou comprovar a identidade de algo, alguém ou de si mesmo, para isso é necessário um conjunto de procedimentos e diligências. O processo de identificação pode ser realizado por diferentes métodos, em casos de cadáveres carbonizados, muitas vezes, o método de escolha para a identificação é o comparativo entre as particularidades odontológicas ante e post mortem. O objetivo deste trabalho é relatar um caso de identificação humana de corpo carbonizado através do método de comparação odontológica. No necrotério do Instituto de Perícias Oficiais local, deram entrada três corpos carbonizados envolvidos em sinistro aeronáutico. O perito odontolegista foi solicitado para participar do processo de identificação de umas das vítimas. O procedimento pericial consistiu na elaboração de um odontograma específico para o corpo não identificado (exame post-mortem) e confronto com os documentos assistenciais (prontuário odontológico) apresentados como sendo da vítima envolvida no acidente (exame ante-mortem). Após a uniformização dos dados Ante Mortem e Post Mortem, os odontogramas foram confrontados gerando o resultado positivo para identificação, destacando-se o registro de várias ausências dentais. Frente ao relato do caso, destaca-se a importância da atuação do cirurgião-dentista no contexto pericial, contribuindo significativamente com a efetividade dos exames realizados, com a celeridade dos resultados, oferecendo uma opção de baixo custo e alta eficiência para a identificação de corpos carbonizados, dispensando outros métodos de identificação

To identify is to determine or prove the identity of something, of someone or of oneself; for this, a set of procedures and diligences are necessary. The identification process can be performed by different methods; in cases of charred corpses, the method of choice for identification is often the comparative between dental and post-mortem dental characteristics. The objective of this work is to report a case of human identification of charred body by means of the method of dental comparison. In the morgue of the local Forensic Institute, three carbonized bodies involved in an aeronautical accident were admitted. A forensic dentist was asked to participate in the process of identifying one of the victims. The expert procedure consisted in the elaboration of a specific dental chart for the unidentified body (post-mortem examination) and confrontation with the assistance documents (dental records) presented as being of the victim involved in the accident (ante-mortem examination). After the standardization of Ante- Mortem and Post-Mortem data, the dental charts were confronted, generating the positive result for identification, highlighting the record of several dental absences. In view of the case report, the importance of the dental surgeon's performance in the expert context is of extreme value, contributing significantly to the effectiveness of the exams performed, with the speed of results, offering a low cost and high efficiency option for the identification of bodies rather than other methods of identification

Primeiros jogos mundiais dos povos indígenas: uma análise da percepção dos espectadores / Ethnic-racial representations and sport: first world games of indigenous peoples / Representaciones étnico-raciales y deporte: primeros juegos mundiales de los pueblos indígenas

O objetivo deste estudo foi analisar os Jogos Mundiais dos Povos Indígenas (JMPI), através do ponto de vista de espectadores que foram ao evento, indígenas e não indígenas, de forma a descrever suas percepções sobre questões políticas e sociais dos jogos. A abordagem é qualitativa e o instrumento utilizado na coleta dos dados foi a entrevista semiestruturada. 15 sujeitos participaram do estudo, escolhidos de forma aleatória. Indícios apontam que os JMPI aproximaram a comunidade global da realidade indígena, mas houve falta de autonomia na formulação do evento por parte das lideranças indígenas, e possibilidade de o evento servir para obscurecer a realidade de violência que os povos indígenas enfrentam no Brasil.

The objective of this study was to analyze the World Indigenous Peoples Games (JMPI), from the point of view of spectators who went to the event, indigenous and non - indigenous, in order to describe their perceptions about political and social issues of the games. The approach is qualitative and the instrument used in the data collection was the semi-structured interview. 15 subjects participated in the study, chosen at random. Findings in the reports indicate that the JMPI brought the global community closer to the indigenous reality, providing opportunities for indigenous peoples around the world. They were understood as an excellent opportunity for multi-ethnic meetings, a space conducive to the verbalization of the demands of the different peoples originating in the world. Have also been reported: lack of autonomy in the formulation of the event by indigenous leaders, and the possibility of the event serving to obscure the reality of violence that indigenous peoples face in Brazil.

El objetivo de este estudio fue analizar los Juegos Mundiales de los Pueblos Indígenas (JMPI), a través del punto de vista de espectadores que fueron al evento, indígenas y no indígenas, para describir sus percepciones sobre cuestiones políticas y sociales de los juegos. El enfoque es cualitativo y el instrumento utilizado en la recolección de los datos fue la entrevista semiestructurada. 15 sujetos participaron del estudio, elegidos de forma aleatoria. Indicios en los relatos apuntan que los JMPI acercaron a la comunidad global de la realidad indígena, oportunizando el congraciamiento de los pueblos indígenas de todo el mundo. Se entendieron como una excelente oportunidad de encuentros multiétinicos, un espacio propicio a la verbalización de las demandas de los diferentes pueblos originarios del mundo. Cuestiones relativas a la falta de autonomía en la formulación del evento por parte de los liderazgos indígenas, y posibilidad de que el evento sirva para oscurecer la realidad de violencia que enfrentan los pueblos indígenas en Brasil, también se han reportado.

Autophagic cell death restricts chromosomal instability during replicative crisis.

Replicative crisis is a senescence-independent process that acts as a final barrier against oncogenic transformation by eliminating pre-cancerous cells with disrupted cell cycle checkpoints1. It functions as a potent tumour suppressor and culminates in extensive cell death. Cells rarely evade elimination and evolve towards malignancy, but the mechanisms that underlie cell death in crisis are not well understood. Here we show that macroautophagy has a dominant role in the death of fibroblasts and epithelial cells during crisis. Activation of autophagy is critical for cell death, as its suppression promoted bypass of crisis, continued proliferation and accumulation of genome instability. Telomere dysfunction specifically triggers autophagy, implicating a telomere-driven autophagy pathway that is not induced by intrachromosomal breaks. Telomeric DNA damage generates cytosolic DNA species with fragile nuclear envelopes that undergo spontaneous disruption. The cytosolic chromatin fragments activate the cGAS-STING (cyclic GMP-AMP synthase-stimulator of interferon genes) pathway and engage the autophagy machinery. Our data suggest that autophagy is an integral component of the tumour suppressive crisis mechanism and that loss of autophagy function is required for the initiation of cancer.

Understanding the dipyrone oxidation allying electrochemical and computational approaches.

Electroanalytical methodology by boron-doped diamond electrode (BDDE) associated to the square-wave voltammetry (SWV) for the determination of hydrolyzed dipyrone (DIP) in commercial formulations, raw natural waters and in human urine was developed. Through cyclic voltammetry (CV), it was shown that the oxidation of the DIP on the BDDE was irreversible with diffusional control. Computational studies suggested that the oxidation mechanism of DIP occurred with participation of two electrons and one proton. The analytical curves were obtained for concentrations of DIP ranging from 1.0 × 10-6 to 6.5 × 10-5 mol L-1 (r = 0.9994). The values of detection limit (LOD) and quantification limit (LOQ) of DIP were calculated from SWV and found to be 2.6 × 10-7 mol L-1 and 8.8 × 10-7 mol L-1. The methodology was effectively applied to real samples with the values of calculated recoveries varying between 91.0% and 117.3% and validated by iodometric titration experiments whose values were between 93.3% and 106.9%. The proposed methodology with BDDE represents an alternative tool and it has advantageous, such as very easy handling, low cost, no need for modification, low detection limit. Furthermore, it can be used for the routine analysis of DIP in different real samples.

Chitosan-magnetite nanocomposite as a sensing platform to bendiocarb determination.

A novel platform for carbamate-based pesticide quantification using a chitosan/magnetic iron oxide (Chit-Fe3O4) nanocomposite as a glassy carbon electrode (GCE) modifier is shown for an analytical methodology for determination of bendiocarb (BND). The BND oxidation signal using GCE/Chit-Fe3O4 compared with bare GCE was catalyzed, showing a 37.5% of current increase with the peak potential towards less positive values, showing method's increased sensitivity and selectivity. Using square-wave voltammetry (SWV), calibration curves for BND determination were obtained (n = 3), and calculated detection and quantification limits values were 2.09 × 10-6 mol L-1 (466.99 ppb) and 6.97 × 10-6 mol L-1 (1555.91 ppb), respectively. The proposed electroanalytical methodology was successfully applied for BND quantification in natural raw waters without any sample pretreatment, proving that the GCE/Chit-Fe3O4 modified electrode showed great potential for BND determination in complex samples. ᅟ Graphical abstract.

Dispersion of multi-walled carbon nanotubes in [BMIM]PF6 for electrochemical sensing of acetaminophen.

The influence of functionalized multi-walled carbon nanotubes (fMWCNT) in the presence of 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF6) in different ratios was investigated on the acetaminophen (ACOP) electrochemical determination. The electrochemical behavior of the ACOP exhibited a pair of well-defined redox peaks, suggesting that the reversibility of ACOP was significantly improved in comparison to irreversible oxidation peak on bare GCE. The redox process was controlled by adsorption, involves two electrons and the value of apparent rate constant (ks) was equal to 14.7 s-1 ±â€¯3.6 s-1. The analytical curves were obtained for concentrations of ACOP ranging from 0.3 to 3.0 µmol L-1. The values of the detection limit were calculated from SWV and found to be 6.73 × 10-8 mol L-1. The proposed electrochemical sensor exhibited good stability and reproducibility and was applied for ACOP determination in tablets (Tylenol® and Tylenol®DC) with satisfactory results.

The effect of water on the physicochemical properties of an ethylene glycol and choline chloride mixture containing Cu2+ ions: electrochemical results and dynamic molecular simulation approach.

The effect of water on the physicochemical properties of an ethylene glycol and choline chloride mixture containing Cu2+ ions was investigated by electrochemical techniques and molecular dynamics simulation. The experiments and computational calculations were carried out by increasing the water content from 0 up to 10% (v/v). The cyclic voltammetry and chronopotentiometry techniques showed that the diffusion coefficient of Cu2+ ions increased and that the peak potentials for both the Cu2+/Cu+ and Cu+/Cu redox couples shifted towards more positive potentials with the increase in the water content in the solution. The molecular dynamics simulation indicated that the water molecules replaced the ethylene glycol molecules that were coordinated with Cu2+ ions, while the interactions between Cu2+ and Cl- ions were not influenced by the presence of water.