Sealing ability of two root-end filling materials at different retro-preparation lengths.

PURPOSE: The aim of the present study was to evaluate the sealing performance, expressed as microleakage (ML), of two root-end filling materials when used at different retro-preparation lengths. METHODS: Fifty single-rooted human teeth were collected for the study. The teeth were cut at the cement-enamel junction and endodontic treatment was performed. Each root was cut at 3 mm from the apex and then stored in wet condition. The teeth were divided into three groups according to the retro-preparation length: control group (no retro-preparation); group 1 (retro-preparation of 3 mm); group 2 (retro-preparation of 9 mm). The teeth were equally allocated to either Biodentine or Super EBA treatment group. The teeth were immersed in 3% methylene blue dye solution for 24 h. The samples were split longitudinally and the depth of dye penetration was examined through light microscopy. RESULTS: No significant statistical differences were found at different retro-preparation lengths (P > 0.05). Differences were found between materials (P > 0.05). CONCLUSION: Biodentine showed significantly lower ML when compared to Super-EBA and no statistical significative differences were observed when samples were retro-prepared at 3 mm or 9 mm.

Cyclic fatigue and torsional resistance evaluation of Reciproc R25 instruments after simulated clinical use.

BACKGROUND: To assess the influence of multiple uses on the cyclic flexural fatigue and torsional resistance of Reciproc R25 (REC25) instruments. METHODS: Overall, 256 canals (two for each mandibular molar) were prepared using Reciproc R25 instruments. A total of 96 25-mm-long Reciproc R25 instruments were divided in six groups (N.=8) on the basis of different number of canals shaped: new instruments (control group), one, two, three, four, or six canals shaped during simulated clinical use for other groups respectively. Sixteen files were used for each of the six groups (eight instruments for cyclic fatigue test and eight ones for torsional test). Times to fracture (TtF) for cyclic fatigue and ultimate torsional strength and angle of rotation to fracture for torsional resistance were recorded. Data were statistically evaluated by the analysis of variance (ANOVA) with P<0.05. RESULTS: As for cyclic fatigue resistance, there was no significant difference between new REC25 and REC25 used in one, two and three canals respectively (P>0.05). REC25 used in four and six canals showed significant lower times to fracture when compared with other groups (P<0.05) with REC25 used in six canals showing the significant lowest times to fracture (P<0.05). Considering torsional resistance parameters, no significant difference was observed between the new and used instruments (P>0.05). CONCLUSIONS: REC25 files showed a significant decrease in cyclic fatigue resistance only when used in four or more molar canals while their torsional behavior was not affected by multiple uses.

Push-out Bond Strength of Fiber Posts Cemented Using New Universal Adhesives on Etched and Nonetched Intraradicular Dentin.

AIM: The aim of this study was to assess if the curing mode and the etching mode could affect the push-out bond strength of different post cementation systems using two universal adhesives. MATERIALS AND METHODS: A total of 100 single-rooted teeth were divided into Prime & Bond Elect (PBE), Prime & Bond Active (PBA), and Prime & Bond XP (PBXP) as a control. The PBE, PBA, and PBXP were used in the self-etch (SE) and etch and rinse (E&R) mode. Post cementation was performed using Core X flow used in dark-cure and in light-cure. Data were analyzed using the one-way ANOVA test and post hoc Bonferroni and Dunnet tests. The Student's t test was performed to find significance between two independent groups. RESULTS: Bond strength was significantly influenced by the adhesive strategies. The PBE and PBA obtained higher values when used in the SE mode (p < 0.001). Light-cured groups obtained significantly higher values (p < 0.001) compared to dark-cured groups. The post space region also had a significant effect on the bond strength; the apical third recorded lower values in all groups (p < 0.001). CONCLUSION: The PBA and PBE universal adhesives obtained higher PBS values when used in the SE mode and followed by the light-curing of resin cement. CLINICAL SIGNIFICANCE: Universal adhesives represent a good alternative to the conventional total-etch adhesive system for fiber post cementation. Since they perform better in SE, they would be recommendable in clinical practice, as they can be used with a simplified technique.

Antimicrobial efficacy of cordless sonic or ultrasonic devices on Enterococcus faecalis-infected root canals.

AIM: The aim of the present study was to evaluate the immediate and residual antimicrobial activity of cordless sonic or ultrasonic devices on Enterococcus faecalis (E faecalis)-infected teeth. METHODS: A total of 140 single-rooted extracted teeth with E faecalis were grouped as follows (N = 15): conventional syringe irrigation with 3% sodium hypochlorite activated by ultrasonic device (group 1) or cordless ultrasonic device (group 2), EndoActivator (group 3) or without activation (group 4), conventional syringe irrigation with sterile bi-distilled water and ultrasonic device (group 5), EndoUltra (group 6), EndoActivator (group 7), or without activation (group 8). The remaining 20 teeth were used for positive and negative controls. Colony-forming units (CFU) and turbidity were recorded from bacteriological samples taken before and after irrigation and after 24 hours of re-incubation. Data were statistically analyzed using Student's t test, Mann-Whitney test, Kruskal-Wallis test, and Dunn's multiple comparison tests (P < .05). RESULTS: Groups 1 and 2 showed higher antimicrobial efficacy than groups 3 and 4 (P < .05). No bacteria reduction was found in groups 5-8 (P > .05). After 24 hours, regrowth of bacteria was not significantly different for all groups (P > .05). CONCLUSIONS: Ultrasound produced lower CFU and turbidity after treatment and after re-incubation of 24 hours than sonic or no activation.

Influence of proper or reciprocating optimum torque reverse kinematics on cyclic fatigue of four single files.

AIM: The aim of the present study was to evaluate the resistance to cyclic fatigue of heat-treated single files used in proper or in reciprocating optimum torque reverse motions. METHODS: Ninety-six HyFlex electrical discharge machining (EDM), Reciproc R25 (Rec), WaveOne Primary (WO), and WaveOne Gold (WOG) Primary were used. Resistance to cyclic fatigue was determined by recording time to fracture in a stainless steel artificial canal. Differential scanning calorimetric analysis was performed. Data were analyzed by two-way analysis of variance and Bonferroni post-hoc tests at 0.05. RESULTS: In both movements, HyFlex EDM showed higher time to fracture than other files (P < 0.0001). Rec showed higher time to fracture than WO (P < 0.0001), while WOG showed higher cyclic fatigue resistance than Rec and WO with both movements (P < 0.005). CONCLUSIONS: Reciprocating optimum torque reverse motion improved significantly cyclic fatigue resistance of gold and controlled memory wire single files.

Shaping effectiveness and surface topography of reciprocating files after multiple simulated uses.

This in vitro study assessed morphological changes and efficiency of reciprocating files after multiple uses. Sixty standardized Endo Training Blocks and 10 ReciprocR25 files were selected (six blocks for each file). Each file was its own control (before use vs. after each instrumentation). The instruments were used according to the manufacturer's instructions, and scanning electron microscopy was used to observe fatigue cracks, metal strips/metal flash, pitting, fretting, debris, disruption of the cutting edge, and plastic deformations after each instrumentation. The presence of seven wear variables was scored semiquantitatively by viewing micrographs collected before and after use. The prepared areas in resin blocks were calculated and compared by using AutoCAD software. The control group had significantly lower values for all wear variables except fretting and plastic deformation. The presence of fatigue cracks and metal strips/metal flash significantly differed between unused instruments and instruments used four or five times, in all observed sections. The area of instrumented Endo Training Blocks significantly differed in relation to the number of instrument uses. The Reciproc files wore progressively, and repeated use affected their shaping efficiency in simulated canals.

Effect of framework design and firing cycle on the success rate of bilayered zirconia crowns.

OBJECTIVE: This study investigated the effect of framework design and modification of porcelain firing cycle on the occurrence of fractures of metal-ceramic and bilayered zirconia crowns after cyclic loading. METHOD AND MATERIALS: Twenty-four zirconia crowns with two different apicocoronal incisal veneering porcelain lengths (ZS = 2.0 mm and ZL = 4.0 mm) were fabricated. Twenty-four metal-ceramic crowns with the same apicocoronal incisal veneering porcelain lengths (MS = 2.0 mm and ML = 4.0 mm) served as the control. All the specimens (n = 48) were thermally and mechanically cycle-loaded (49 N) for 1,200,000 cycles, and then evaluated for cracks and/or bulk fractures with an optical stereomicroscope (×10). In the second part of the study, the influence of firing cycle modification on the success rate of twenty-four bilayered zirconia crowns was also evaluated. Finally, two specimens from each group (n = 12) were sectioned and analyzed using a scanning electron microscope. Load-to-failure of the specimen that did not present bulk fracture was further assessed using a universal testing machine. Statistical analysis was performed with Fisher exact test and Kruskal-Wallis test. RESULTS: A statistically significant lower occurrence of fractures (P < .05) was found after cyclic loading for bilayered zirconia versus metal-ceramic crowns. Modification of the firing cycle did not significantly influence the outcome after cyclic loading for zirconia bilayered crowns (P > .05). CONCLUSION: Within the limitations of this study, porcelain-firing cycle with an extended cooling time did not appear to influence the occurrence of cracks and fractures of bilayered zirconia crowns.

Influence of Access Cavity Preparation and Remaining Tooth Substance on Fracture Strength of Endodontically Treated Teeth.

INTRODUCTION: The purpose of this study was to assess the impact of access cavity preparation and the remaining tooth substance on the fracture strength of endodontically treated teeth. METHODS: One hundred sound mandibular first and second molar teeth were selected and divided into 1 control and 9 test groups (n = 10/group) as follows: control group, intact teeth; group 1, teeth prepared with traditional endodontic access cavity (TEC); group 2, teeth prepared with conservative endodontic access cavity (CEC); group 3, teeth prepared with truss endodontic access cavity (TREC); group 4, TEC + 3 residual walls; group 5, CEC + 3 residual walls; group 6, TREC + 3 residual walls; group 7, TEC + 2 residual walls; group 8, CEC + 2 residual walls; and group 9, TREC + 2 residual walls. After access cavity preparation, all test teeth were endodontically treated and restored. The specimens were then loaded to fracture in a universal loading machine (Triaxial Tester T400 Digital; Controls srl, Cernusco, Italy), and fracture values were recorded in newtons. The data were analyzed with 2-way analysis of variance and Tukey post hoc analysis for multiple comparisons (P < .05). RESULTS: Intact teeth were more resistant to fracture than the teeth in all the test groups. No statistically significant differences were found between the 3 tested access cavities (P > .05). Significant differences were observed between the different number of residual walls (P < .05). CONCLUSIONS: TRECs do not increase the fracture strength of endodontically treated teeth in comparison with CECs and TECs. Moreover, the loss of mesial and distal ridges reduced the fracture strength of teeth significantly.

Influence of Continuous or Reciprocating Optimum Torque Reverse Motion on Cyclic Fatigue Resistance of Two Single-File Nickel-Titanium Rotary Instruments.

OBJECTIVE: Different single-file instruments and kinematics have been introduced on the market. It is important to know the cyclic fatigue performance of these instruments in these new kinematics such as reciprocation of Optimum Torque Reverse (OTR) motion. The aim of this study was to evaluate the resistance to cyclic fatigue of F6 SkyTaper and OneShape used in continuous rotation (proper rotation) or in reciprocating OTR motion. METHODS: A total of forty-eight nickel-titanium files were tested. Twenty-four instruments of both brands were divided into two groups (n=12) on the basis of the motion tested: continuous rotation (group 1) or reciprocating OTR motion (group 2). Resistance to cyclic fatigue was determined by recording time to fracture (TtF) in a stainless steel artificial canal with a 60° angle of curvature and 5 mm radius of curvature. Data were analysed by two-way analysis of variance and post-hoc Bonferroni tests for multiple comparisons with P<0.05 as the level of significance. RESULTS: F6 SkyTaper showed higher TtF compared with OneShape, both in continuous and in OTR motion (P<0.0001). The two tested instruments showed higher cyclic fatigue resistance in reciprocating OTR motion than continuous rotation (P<0.0001). CONCLUSION: OTR motion significantly improves cyclic fatigue resistance of the tested instruments. In addition, F6 SkyTaper showed higher cyclic fatigue resistance than OneShape in both motions.