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1.
Opt Express ; 31(3): 3504-3519, 2023 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-36785342

RESUMEN

Nowadays, atom-based quantum sensors are leaving the laboratory towards field applications requiring compact and robust laser systems. Here we describe the realization of a compact laser system for atomic gravimetry. Starting with a single diode laser operating at 780 nm and adding only one fiber electro-optical modulator, one acousto-optical modulator and one laser amplifier we produce laser beams at all the frequencies required for a Rb-87 atomic gravimeter. Furthermore, we demonstrate that an atomic fountain configuration can also be implemented with our laser system. The modulated system reported here represents a substantial advance in the simplification of the laser source for transportable atom-based quantum sensors that can be adapted to other sensors such as atomic clocks, accelerometers, gyroscopes or magnetometers with minor modifications.

2.
Nutr Hosp ; 31 Suppl 1: 99-118, 2015 Feb 07.
Artículo en Español | MEDLINE | ID: mdl-25659062

RESUMEN

Prebiotics are non-digestible food ingredients (oligosaccharides) that reach the colon and are used as substrate by microorganisms producing energy, metabolites and micronutrients used for the host; in addition they also stimulate the selective growth of certain beneficial species (mainly bifidobacteria and lactobacilli) in the intestinal microbiota. In this article, a multidisciplinary approach to understand the concept of prebiotic carbohydrates, their properties and beneficial effects in humans has been carried out. Definitions of prebiotics, reported by relevant international organizations and researchers, are described. A comprehensive description of accepted prebiotics having strong scientific evidence of their beneficial properties in humans (inulin-type fructans, FOS, GOS, lactulose and human milk oligosaccharides) is reported. Emerging prebiotics and those which are in the early stages of study have also included in this study. Taken into account that the chemical structure greatly influences carbohydrates prebiotic properties, the analytical techniques used for their analysis and characterization are discussed. In vitro and in vivo models used to evaluate the gastrointestinal digestion, absorption resistance and fermentability in the colon of prebiotics as well as major criteria to design robust intervention trials in humans are described. Finally, a comprehensive summary of the beneficial effects of prebiotics for health at systemic and intestinal levels is reported. The research effort on prebiotics has been intensive in last decades and has demonstrated that a multidisciplinary approach is necessary in order to claim their health benefits.


Los prebióticos son ingredientes alimentarios no digeribles (oligosacáridos) que llegan al colon y sirven de sustrato a los microorganismos, originando energía, metabolitos y micronutrientes utilizados por el hospedador y estimulando el crecimiento selectivo de determinadas especies beneficiosas (principalmente, bifidobacterias y lactobacilos) de la microbiota intestinal. En este artículo se realiza una revisión sobre los carbohidratos prebióticos desde diferentes perspectivas, comenzando por las definiciones de prebióticos formuladas a lo largo de los últimos treinta años por científicos y diferentes organismos internacionales. Se realiza una descripción detallada de los prebióticos aceptados, como tales, que presentan propiedades beneficiosas fundamentadas en estudios llevados a cabo en humanos (fructanos tipo inulina y FOS; GOS, lactulosa y oligosacáridos de leche humana), los que se consideran prebióticos emergentes y aquellos que se encuentran en fases iniciales de estudio. Además y teniendo en cuenta que la estructura química de los carbohidratos influye notablemente en sus propiedades prebióticas, se describen las técnicas más utilizadas para su análisis y caracterización. Asimismo, se detallan los modelos in vitro e in vivo más utilizados para estudiar la resistencia de los prebióticos a la digestión y la absorción gastrointestinal, la fermentación de los prebióticos en el colon así como los criterios a tener en cuenta para llevar a cabo ensayos de intervención en humanos. Por último se realiza una amplia descripción de los efectos beneficiosos de los prebióticos para la salud a nivel intestinal y sistémico. Como conclusión, podría decirse que la investigación existente hasta el momento, sobre prebióticos, es extensa y pone de manifiesto que es necesario considerar un gran número de factores para poder atribuir alegaciones de salud a un prebiótico.


Asunto(s)
Prebióticos , Carbohidratos , Fibras de la Dieta , Microbioma Gastrointestinal , Tracto Gastrointestinal/microbiología , Humanos , Microbiota
3.
Nutr. hosp ; 31(supl.1): 99-118, feb. 2015. ilus, tab
Artículo en Español | IBECS | ID: ibc-133223

RESUMEN

Los prebióticos son ingredientes alimentarios no digeribles (oligosacáridos) que llegan al colon y sirven de sustrato a los microorganismos, originando energía, metabolitos y micronutrientes utilizados por el hospedador y estimulando el crecimiento selectivo de determinadas especies beneficiosas (principalmente, bifidobacterias y lactobacilos) de la microbiota intestinal. En este artículo se realiza una revisión sobre los carbohidratos prebió- ticos desde diferentes perspectivas, comenzando por las definiciones de prebióticos formuladas a lo largo de los últimos treinta años por científicos y diferentes organismos internacionales. Se realiza una descripción detallada de los prebióticos aceptados, como tales, que presentan propiedades beneficiosas fundamentadas en estudios llevados a cabo en humanos (fructanos tipo inulina y FOS; GOS, lactulosa y oligosacáridos de leche humana), los que se consideran prebióticos emergentes y aquellos que se encuentran en fases iniciales de estudio. Además y teniendo en cuenta que la estructura química de los carbohidratos influye notablemente en sus propiedades prebióticas, se describen las técnicas más utilizadas para su análisis y caracterización. Asimismo, se detallan los modelos in vitro e in vivo más utilizados para estudiar la resistencia de los prebióticos a la digestión y la absorción gastrointestinal, la fermentación de los prebióticos en el colon así como los criterios a tener en cuenta para llevar a cabo ensayos de intervención en humanos. Por último se realiza una amplia descripción de los efectos beneficiosos de los prebióticos para la salud a nivel intestinal y sistémico. Como conclusión, podría decirse que la investigación existente hasta el momento, sobre prebióticos, es extensa y pone de manifiesto que es necesario considerar un gran número de factores para poder atribuir alegaciones de salud a un prebiótico (AU)


Prebiotics are non-digestible food ingredients (oligosaccharides) that reach the colon and are used as substrate by microorganisms producing energy, metabolites and micronutrients used for the host; in addition they also stimulate the selective growth of certain beneficial species (mainly bifidobacteria and lactobacilli) in the intestinal microbiota. In this article, a multidisciplinary approach to understand the concept of prebiotic carbohydrates, their properties and beneficial effects in humans has been carried out. Definitions of prebiotics, reported by relevant international organizations and researchers, are described. A comprehensive description of accepted prebiotics having strong scientific evidence of their beneficial properties in humans (inulin-type fructans, FOS, GOS, lactulose and human milk oligosaccharides) is reported. Emerging prebiotics and those which are in the early stages of study have also included in this study. Taken into account that the chemical structure greatly influences carbohydrates prebiotic properties, the analytical techniques used for their analysis and characterization are discussed. In vitro and in vivo models used to evaluate the gastrointestinal digestion, absorption resistance and fermentability in the colon of prebiotics as well as major criteria to design robust intervention trials in humans are described. Finally, a comprehensive summary of the beneficial effects of prebiotics for health at systemic and intestinal levels is reported. The research effort on prebiotics has been intensive in last decades and has demonstrated that a multidisciplinary approach is necessary in order to claim their health benefits (AU)


Asunto(s)
Humanos , Prebióticos/análisis , Suplementos Dietéticos/análisis , Sustratos para Tratamiento Biológico/análisis , Microbiota/inmunología , Lactulosa/análisis , Inulina/análisis , Oligosacáridos/análisis
4.
J Pediatr Gastroenterol Nutr ; 56(5): 560-8, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23274339

RESUMEN

OBJECTIVE: The objective of this work was to evaluate the effect of Holder pasteurisation of human colostrum on a variety of microbiological, biochemical, and immunological parameters. METHODS: Colostrum samples from 10 donors, and 8 samples of mature milk used as controls, were heated at 62.5°C for 30 minutes. Bacterial counts and the concentration of furosine, lactose, myoinositol, glucose, lactulose, cytokines, and immunoglobulins were determined before and after the heat treatment. RESULTS: Mean bacterial counts in nonpasteurised colostrum samples oscillated between 2.72 and 4.13 log10 colony-forming units per millilitre in the agar media tested. Holder pasteurisation led to the destruction of the bacteria originally present in the samples. Furosine was detected in all samples before pasteurisation and increased significantly after the heat treatment (from 6.60 to 20.59 mg/100 g protein). Lactulose content was below the detection limit in nonpasteurised colostrum, but it was detected in all samples and quantified in 7 of them (from 10.68 to 38.02 mg/L) after Holder pasteurisation. Lactose, glucose, and myoinositol concentrations did not change after Holder pasteurisation. The concentrations of most cytokines and immunoglobulins were significantly higher in colostrum than in mature milk samples. Immunoglobulin content, both in colostrum and in milk samples, was reduced during pasteurisation, whereas, among cytokines, only macrophage inflammatory protein-1ß, interleukin-7, and granulocyte-macrophage-colony-stimulating factor concentrations were affected by this heat treatment. CONCLUSIONS: Lactulose and furosine content could be used as heat treatment indicators in colostrum samples. Holder pasteurisation modified the immunological profile of both colostrum and mature milk.


Asunto(s)
Calostro , Citocinas/análisis , Inmunoglobulinas/análisis , Lactulosa/análisis , Lisina/análogos & derivados , Pasteurización/métodos , Carga Bacteriana , Quimiocina CCL4/análisis , Calostro/química , Calostro/inmunología , Calostro/microbiología , Femenino , Factor Estimulante de Colonias de Granulocitos y Macrófagos/análisis , Calor , Humanos , Interleucina-7/análisis , Lisina/análisis , Leche Humana/química , Leche Humana/inmunología , Leche Humana/microbiología
5.
Phys Rev Lett ; 109(4): 043602, 2012 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-23006087

RESUMEN

We implement a noiseless optical amplifier using a phase-sensitive four-wave mixing process in rubidium vapor. We observe performance near the quantum limit for this type of amplifier over a range of experimental parameters and show that the noise figure is always better than would be obtained with a phase-insensitive amplifier with the same gain. Additionally, we observe that the amplifier supports hundreds of spatial modes, making it possible to amplify complex two-dimensional spatial patterns with less than a 10% degradation of the input signal-to-noise ratio for gains up to 4.6. To confirm the multimode character of the amplifier, we study the noise figure as a function of spatially-varying losses. Additionally, we investigate the spatial resolution of the amplifier and show that it supports a range of spatial frequencies from 1.3 to more than 35 line pairs per millimeter.

6.
J Small Anim Pract ; 48(12): 702-4, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17850279

RESUMEN

A five-year-old, female spayed Labrador retriever was presented for further investigation of an intra-abdominal mass. Abdominal exploration showed a large mass arising from the right ureter and a ureteronephrectomy was performed. Histopathology of the ureteral mass was consistent with a spindle cell sarcoma. The patient recovered well, but five months later was diagnosed with another tumour, this time in her left abdominal wall. The owners decided not to pursue further treatment and euthanasia was performed a month later.


Asunto(s)
Enfermedades de los Perros/diagnóstico , Sarcoma/veterinaria , Neoplasias Ureterales/veterinaria , Animales , Diagnóstico Diferencial , Enfermedades de los Perros/diagnóstico por imagen , Enfermedades de los Perros/patología , Perros , Femenino , Radiografía , Sarcoma/diagnóstico , Neoplasias Ureterales/diagnóstico
7.
J Agric Food Chem ; 55(9): 3346-50, 2007 May 02.
Artículo en Inglés | MEDLINE | ID: mdl-17394342

RESUMEN

The viability of the purification of lactulose from a mixture with lactose [70:30 (w/w)] using pressurized liquid extraction (PLE) at 1500 psi for 30 min was studied. Different temperatures (from 40 to 130 degrees C) and proportions of ethanol:water (70:30, 80:20, 90:10, 95:5, and 100:0) as the extraction solvent were assayed. Lactose and lactulose were measured by gas chromatographic analysis as their trimethylsilyl derivatives. Data were fitted through multiple linear regressions to different quadratic models to describe both the extraction yield (in terms of mg of lactulose) and the purity of the lactulose extracted. The optimum extraction conditions provided by the model were as follows: extraction temperature, 40 degrees C; and solvent composition, 70:30 ethanol:water. PLE extraction under the optimized conditions was also applied to purify lactulose from lactose in a synthesis mixture. To our knowledge, this is the first time that PLE has been tested for extraction and purification of lactulose from its mixture with lactose; this technique showed several advantages over classical methods such as the short extraction time and the low solvent consumption.


Asunto(s)
Lactosa/química , Lactulosa/aislamiento & purificación , Fraccionamiento Químico/métodos , Cromatografía de Gases , Etanol , Lactosa/análisis , Lactulosa/análisis , Presión , Temperatura , Agua
8.
J Dairy Sci ; 89(5): 1384-9, 2006 May.
Artículo en Inglés | MEDLINE | ID: mdl-16606709

RESUMEN

A method is described for selective removal of undenatured beta-lactoglobulin from cheese whey based on interactions between whey proteins and chitosan. Whey was previously clarified at pH 4.5 with addition of chitosan (25 mg/100 mL), and selective removal of beta-lactoglobulin was studied in the pH interval 4.6 to 6.5. Addition of chitosan caused selective precipitation of beta-lactoglobulin that increased with pH. The content of beta-lactoglobulin in whey decreased as the amount of chitosan added was increased. At pH 6.2, addition of 1.9 to 3.0 mg/mL of chitosan led to complete removal of beta-lactoglobulin, whereas at least 80% of the rest of whey proteins remained in solution. The production of cheese whey without beta-lactoglobulin could help to expand the applications of dairy by-products in food processing, and to isolate hypoallergenic whey protein concentrates.


Asunto(s)
Queso/análisis , Quitosano , Lactoglobulinas/aislamiento & purificación , Proteínas de la Leche/química , Fraccionamiento Químico , Cromatografía Líquida de Alta Presión , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno , Proteína de Suero de Leche
9.
J Agric Food Chem ; 53(4): 1201-4, 2005 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-15713041

RESUMEN

The use of chitosan, a partially deacetylated chitin, to fractionate aqueous solutions of caseinmacropeptides (CMPs) was studied. The polycationic character of chitosan at acidic pH values allows the formation of complexes with negatively charged CMP molecules, inducing their flocculation. Glycosylated CMP (GMP) has higher affinity for chitosan than nonglycosylated forms (NGMP). The carboxylic groups in the carbohydrate moiety of the GMP increase the negative charge of the molecule and may play a role in the selective precipitation. At pH 5.0, 0.08 mg/mL of chitosan completely removed the GMP whereas 70% of NGMP remained in solution. As the pH increased, the amount of chitosan to ensure complete removal of GMP increased up to 0.19 and 0.34 mg/mL for pH 6.0 and 6.6, respectively.


Asunto(s)
Caseínas/química , Caseínas/aislamiento & purificación , Quitosano/química , Fragmentos de Péptidos/química , Fragmentos de Péptidos/aislamiento & purificación , Caseínas/metabolismo , Precipitación Química , Cromatografía Líquida de Alta Presión , Quimosina/metabolismo , Glicosilación , Concentración de Iones de Hidrógeno , Neuraminidasa/metabolismo , Soluciones
10.
Vet Rec ; 153(21): 648-52, 2003 Nov 22.
Artículo en Inglés | MEDLINE | ID: mdl-14667085

RESUMEN

Twenty-six dogs with parasitologically confirmed leishmaniasis and abnormalities of gait were studied to determine the most common radiological patterns of bone and joint lesions. The clinical findings included either lameness, joint pain and crepitation, soft tissue swelling and/or muscle atrophy. Bone lesions were observed radiographically in 12 of the 26 dogs; the radius and ulna were affected in seven, the tibia in six and the femur in six. Joint lesions were observed radiographically in 15 of the 26 dogs; the carpus and stifle were affected in all 15, and the tarsus in nine. There was a tendency for the bones and joints to be affected bilaterally. The radiographic patterns observed were different in the long bones and the joints. In the long bones, the most common pattern was periosteal and intramedullary proliferation, involving the diaphyses and related to the nutrient foramen; in the joints, two patterns, either non-erosive or erosive polyarthritis with soft-tissue swelling, were observed. The changes observed in the synovial fluid were associated in most cases with osteolytic lesions. However, Leishmania organisms were identified in the synovial fluid from joints without bony radiographic changes.


Asunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Leishmaniasis/veterinaria , Animales , Enfermedades de los Perros/patología , Perros , Femenino , Articulaciones/patología , Cojera Animal/parasitología , Cojera Animal/patología , Leishmania/aislamiento & purificación , Leishmaniasis/diagnóstico por imagen , Leishmaniasis/patología , Masculino , Radiografía , Radio (Anatomía)/diagnóstico por imagen , Rodilla de Cuadrúpedos/diagnóstico por imagen , Rodilla de Cuadrúpedos/patología , Líquido Sinovial/química , Líquido Sinovial/citología , Líquido Sinovial/parasitología , Tibia/diagnóstico por imagen , Cúbito/diagnóstico por imagen
11.
J Agric Food Chem ; 49(11): 5228-31, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11714308

RESUMEN

The presence of Amadori compounds in commercial dehydrated fruits has been shown through HPLC analysis of the corresponding 2-furoylmethyl-amino acids obtained by acid hydrolysis. Furosine (2-furoylmethyl-lysine) was the main 2-furoylmethyl derivative observed in dried figs and apricot samples, whereas in prunes and dates similar amounts of furosine and 2-furoylmethyl-gamma-aminobutyric acid were detected. A considerable variation of 2-furoylmethyl-amino acid contents among commercial raisin samples was observed. 2-Furoylmethyl-gamma-aminobutyric acid and 2-furoylmethyl-arginine, the most abundant 2-furoylmethyl-amino acids, ranged between 9.9 and 75.8 mg/100 g sample and 10.0 and 62.5 mg/100 g sample, respectively. Most of the Amadori compounds present in raisins seem to have originated during the commercial shelf life period rather than during processing. Determination of 2-furoylmethyl-amino acids could be used as a method of controlling commercial dehydrated fruit and selecting storage conditions.


Asunto(s)
Aminoácidos/química , Frutas/química , Aminoácidos/análisis , Cromatografía Líquida de Alta Presión , Reacción de Maillard , Espectrometría de Masas , Espectrofotometría Ultravioleta , Agua/química
12.
J Agric Food Chem ; 48(9): 4217-20, 2000 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10995340

RESUMEN

The formation of 2-furoylmethyl derivatives of GABA (2-FM-GABA) and arginine (2-FM-Arg) as early indicators of nonenzymatic browning in different types of orange juice was studied. In dehydrated orange juice, the presence of 2-FM-GABA and 2-FM-Arg was detected from the first day of storage at 30 degrees C. In this type of juice, the content of these two compounds increased with temperature (30, 50 degrees C) and time (1-7 days) of storage. A noticeable increase in 5-hydroxymethylfurfural was only observed after 4 days of storage at 50 degrees C. No formation of 2-FM-GABA and 2-FM-Arg was detected in liquid orange juice heated under conditions similar to those used in the industry. These furoylmethyl derivatives were also found in commercial orange juice made from concentrates. A slight increase in their concentration was observed in the two samples stored during 8 months at room temperature. According to the results obtained, 2-FM-GABA and 2-FM-Arg contents could be suitable indicators to assess the main modifications due to Maillard reaction produced during the manufacture and/or storage of orange juice concentrates.


Asunto(s)
Aminobutiratos/química , Arginina/química , Bebidas , Citrus/química , Reacción de Maillard
13.
J Food Prot ; 63(7): 974-5, 2000 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-10914672

RESUMEN

The furosine content in 53 samples of different cheese types (fresh, ripened with molds, artisanal hard-pressed, industrial hard-pressed, and processed) marketed in Spain was analyzed. The lowest furosine values were observed in artisanal hard-pressed cheeses (4.8 to 10.2 mg per 100 g of protein) and ripened with molds cheeses (4.2 to 12.8 mg per 100 g of protein). Industrial hard-pressed cheeses showed furosine values between 3.5 and 43.8 mg per 100 g of protein. Differences between samples may be attributed to the heat-treatment intensity given to cheese milk and to cheesemaking conditions. The highest contents of furosine were observed in processed cheeses (20 to 366.6 mg per 100 g of protein). A wide range of furosine content was found in fresh cheeses (17.9 to 73.6 mg per 100 g of protein), which could be due to the different amounts of dried milk added during the manufacture of cheeses.


Asunto(s)
Queso/análisis , Contaminación de Alimentos/análisis , Lisina/análogos & derivados , Lisina/análisis , Animales , Queso/normas , España
14.
J Agric Food Chem ; 48(2): 468-71, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10691658

RESUMEN

Acid hydrolysis of Amadori compounds yields the corresponding 2-furoylmethylamino acids (2-FM-AA) that can be analyzed by ion-pair HPLC. The relative proportions of the different 2-FM-AA present in the hydrolysates of tomato products were determined to assess their usefulness as indicators of quality. In the lyophilized tomato samples stored at 50 degrees C and a(w) = 0.44 the formation of 2-FM derivatives of alanine, gamma-aminobutyric acid (GABA), asparagine, aspartic acid, glutamic acid, lysine, serine, and threonine was detected. In commercial tomato products the most abundant 2-FM-AA was 2-FM-GABA (from traces to 26.4 mg/100 g of dry matter) followed by 2-FM-lysine (furosine). Differences in 2-FM-AA contents among samples may be related to processing and storage conditions.


Asunto(s)
Furanos/química , Solanum lycopersicum/metabolismo , Alanina/química , Asparagina/química , Ácido Aspártico/química , Cromatografía Líquida de Alta Presión , Liofilización , Ácido Glutámico/química , Hidrólisis , Lisina/química , Reacción de Maillard , Serina/química , Treonina/química , Ácido gamma-Aminobutírico/química
15.
J Agric Food Chem ; 47(10): 4137-9, 1999 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-10552779

RESUMEN

The product of acid hydrolysis of the Amadori compound gamma-N-(1-deoxy-D-fructosyl)aminobutyric acid was isolated and identified by (1)H NMR and (13)C NMR as gamma-N-(2-furoylmethyl)aminobutyric acid. This compound is an analogue to furosine, formed during acid hydrolysis of the corresponding Amadori compound. The retention time of the isolated compound was the same as that of the main peak observed in acid hydrolysates of stored orange juice powder. gamma-N-(2-Furoylmethyl)aminobutyric acid can be a useful indicator of the early stages of Maillard reaction in foods containing free gamma-aminobutyric acid.


Asunto(s)
Aminobutiratos/química , Reacción de Maillard , Aminobutiratos/síntesis química , Conservación de Alimentos , Humanos , Hidrólisis
16.
J Agric Food Chem ; 47(10): 4388-90, 1999 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-10552822

RESUMEN

The formation of furoylmethyl derivatives of amino acids as indicators of the early stages of Maillard reaction in dehydrated orange juices and model systems was studied. In stored dehydrated orange juices, the presence of furoylmethyl derivatives of arginine, asparagine, proline, alanine, glutamic acid, and GABA was detected. Their formation increased with temperature of storage. After 2 weeks at 30 degrees C and a(w) = 0.44, the reconstituted orange juice contained 94 mg/L furoylmethyl derivatives, whereas up to 1215 mg/L was detected in samples stored at 50 degrees C.


Asunto(s)
Bebidas , Citrus/química , Reacción de Maillard , Cromatografía Líquida de Alta Presión , Deshidratación , Humanos
17.
J Agric Food Chem ; 46(1): 277-280, 1998 Jan 19.
Artículo en Inglés | MEDLINE | ID: mdl-10554232

RESUMEN

Maillard reaction in dehydrated orange juice stored at 30 or 50 degrees C and a(w) = 0.44 was studied. The decreases of the total amino acids were 30 and 65% of initial concentration after 14 days of storage at 30 and 50 degrees C, respectively. Storage at 50 degrees C for 14 days caused a decrease of 11.8 g/L of carbohydrates, and glucose was more reactive than fructose. Loss of sucrose due to hydrolysis was also observed. Presence of 1-(N-substituted)amino-1-deoxy-D-fructose compounds in stored dehydrated orange juice was detected by thin-layer chromatography.

18.
J Chromatogr A ; 729(1-2): 99-111, 1996 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-9004966

RESUMEN

A perfusion reversed-phase (RP) HPLC method was developed for the rapid separation of the main bovine whey proteins: alpha-lactalbumin (alpha-LA), serum albumin (BSA) and the genetic variants of beta-lactoglobulin (A and B) (beta-LG A and beta-LG B). For the method development, the influence of factors favouring structural changes of proteins (temperature and organic acid concentration in the mobile phase), gradient and other chromatographic conditions and the mass of protein injected was examined. The optimized method allowed the separation of proteins in about 1.5 min (cycle time 3.5 min) with resolution around 1.0 for the beta-lactoglobulins. The method was applied to the determination of proteins in a whey from raw bovine milk. The precision of the determinations was < or = 3.75 mg per 100 ml (S.D.). With respect to the accuracy, errors < or = 7.0% in the determination of alpha-LA, beta-LG A and beta-LG B were obtained, compared with an RP-HPLC reference method. However, higher errors in the quantification of BSA were found owing to the lack of purity of the peak assigned. In addition, the proposed method has proved to be very useful in the detection of homologous whey proteins from different species (cow, sheep and goat) in milk mixtures.


Asunto(s)
Proteínas de la Leche/aislamiento & purificación , Animales , Calibración , Bovinos , Cromatografía Líquida de Alta Presión , Cabras , Concentración de Iones de Hidrógeno , Lactalbúmina/aislamiento & purificación , Peso Molecular , Perfusión , Albúmina Sérica Bovina/aislamiento & purificación , Ovinos , Especificidad de la Especie , Temperatura , Proteína de Suero de Leche
19.
Z Lebensm Unters Forsch ; 202(1): 15-8, 1996 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-8717091

RESUMEN

Raw cows' and goats' milks were heated by microwave in a continuous flow unit up to temperatures ranging from 73.1 to 96.7 degrees C. The effects of the heat treatments were estimated by measurements of lactose isomerization, protein denaturation, inactivation of alkaline phosphatase and peroxidase and the total bacterial count. Negative phosphatase tests and low bacterial counts, together with low degrees of whey protein denaturation, were achieved under several temperature/time combinations. The results indicate that continuous microwave processing may be an efficient and mild approach for the pasteurization of milk.


Asunto(s)
Microondas , Leche/microbiología , Leche/efectos de la radiación , Fosfatasa Alcalina/antagonistas & inhibidores , Animales , Bacterias/aislamiento & purificación , Bovinos , Femenino , Cabras , Calor , Lactosa/análisis , Lactosa/química , Leche/química , Proteínas de la Leche/análisis , Peroxidasas/antagonistas & inhibidores , Desnaturalización Proteica
20.
Z Lebensm Unters Forsch ; 198(4): 302-6, 1994 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-8197826

RESUMEN

Changes in furosine, undenatured whey protein content, and proteolysis during 90 days storage at 20, 30, and 40 degrees C of UHT-processed milk were studied. Furosine increased as the temperature and storage time increased. The peptide concentration increased during the storage period, being faster at 30 degrees C than at 20 or 40 degrees C. High-performance liquid chromatographic analysis of undenatured whey proteins showed considerable changes in the shape of the peaks during storage.


Asunto(s)
Conservación de Alimentos , Calor , Lisina/análogos & derivados , Proteínas de la Leche/metabolismo , Leche/química , Animales , Cromatografía Líquida de Alta Presión , Concentración de Iones de Hidrógeno , Lisina/metabolismo
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