Protein engineering: a driving force toward synthetic immunology.

The full application of the diverse toolkit of protein engineering has made it easier to control the immune system. In particular, synthetic cytokine variants and engineered immune receptor platforms have shown promise for the treatment of various indications with dysregulated immune function, particularly cancer. Here, we review recent advances in the control of immune cell signaling and therapeutic potency that have employed protein engineering strategies. We further discuss how safety concerns are driving the design of immunotherapeutics toward 'user-defined' control or requiring multiple distinct inputs before a functional response, highlighting emergent control strategies employed for chimeric antigen receptor (CAR) engineering.

Soil contamination and persistence of pollutants following organophosphate sprays and explosions to control red-billed quelea (Quelea quelea).

BACKGROUND: Red-billed quelea (Quelea quelea) are controlled at breeding colonies and roosts by organophosphate sprays or explosions. Contamination with organophosphates after sprays and with petroleum products and phthalates after explosions was assessed. RESULTS: Concentrations in soil of the organophosphate fenthion the day after sprays were uneven (0-29.5 µg g(-1)), which was attributable to excess depositions at vehicle turning points, incorrect positioning of nozzles and poor equipment maintenance. A laboratory study using field-collected samples provided an estimate of 47 days for the half-life of fenthion. After sprays, fenthion persisted in soil for up to 188 days. High concentrations were detected 5 months after negative results at the same sites, providing indirect evidence of leaching. Concentrations of total petroleum hydrocarbons (TPHs) and phthalates ranged from 0.05 to 130.81 (mean 18.69) µg g(-1) and from 0 to 1.62 (mean 0.55) µg g(-1) respectively in the craters formed by the explosions, but declined to means of 0.753 and 0.027 µg g(-1) at 10 m away. One year after an explosion, mean TPHs of 0.865 and mean phthalates of 0.609 were detected. CONCLUSION: Localisation of high concentrations of fenthion likely to have effects on soil biota could be mitigated by improved spray management. Given a half-life in the soil of 47 days for fenthion and the possibility of its leaching months after applications raises concerns about its acceptability. The pollutants left behind after explosions have been quantified for the first time, and, given their long-term persistence, their continued use poses a threat to environmental health.