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1.
Sensors (Basel) ; 24(2)2024 Jan 05.
Artículo en Inglés | MEDLINE | ID: mdl-38257416

RESUMEN

Attention-Deficit/Hyperactivity Disorder (ADHD) is a neurodevelopmental disorder known for its significant heterogeneity and varied symptom presentation. Describing the different subtypes as predominantly inattentive (ADHD-I), combined (ADHD-C), and hyperactive-impulsive (ADHD-H) relies primarily on clinical observations, which can be subjective. To address the need for more objective diagnostic methods, this pilot study implemented a Microsoft Kinect-based Stroop Color-Word Test (KSWCT) with the objective of investigating the potential differences in executive function and motor control between different subtypes in a group of children and adolescents with ADHD. A series of linear mixture modeling were used to encompass the performance accuracy, reaction times, and extraneous movements during the tests. Our findings suggested that age plays a critical role, and older subjects showed improvements in KSWCT performance; however, no significant divergence in activity level between the subtypes (ADHD-I and ADHD-H/C) was established. Patients with ADHD-H/C showed tendencies toward deficits in motor planning and executive control, exhibited by shorter reaction times for incorrect responses and more difficulty suppressing erroneous responses. This study provides preliminary evidence of unique executive characteristics among ADHD subtypes, advances our understanding of the heterogeneity of the disorder, and lays the foundation for the development of refined and objective diagnostic tools for ADHD.


Asunto(s)
Trastorno por Déficit de Atención con Hiperactividad , Adolescente , Niño , Humanos , Trastorno por Déficit de Atención con Hiperactividad/diagnóstico , Proyectos Piloto , Movimiento (Física) , Movimiento , Conducta Impulsiva
2.
Front Cell Infect Microbiol ; 13: 1203991, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37886663

RESUMEN

Introduction: Antimicrobial Resistance is a serious public health problem, which is aggravated by the ability of the microorganisms to form biofilms. Therefore, new therapeutic strategies need to be found, one of them being the use of cationic dendritic systems (dendrimers and dendrons). Methods: The aim of this study is to analyze the in vitro antimicrobial efficacy of six cationic carbosilane (CBS) dendrimers and one dendron with peripheral ammonium groups against multidrug-resistant bacteria, some of them isolated hospital strains, and their biofilms. For this purpose, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and minimum eradication biofilm concentration (MBEC) studies were carried out. In addition, the cytotoxicity on Hela cells of those compounds that proved to be the most effective was analyzed. Results: All the tested compounds showed in vitro activity against the planktonic forms of methicillin-resistant Staphylococcus aureus and only the dendrimers BDSQ017, BDAC-001 and BDLS-001 and the dendron BDEF-130 against their biofilms. On the other hand, only the dendrimers BDAC 001, BDLS-001 and BDJS-049 and the dendron BDEF-130 were antibacterial in vitro against the planktonic forms of multidrug-resistant Pseudomonas aeruginosa, but they lacked activity against their preformed biofilms. In addition, the dendrimers BDAC-001, BDLS-001 and BDSQ-017 and the dendron BDEF-130 exhibited a good profile of cytotoxicity in vitro. Discussion: Our study demonstrates the possibility of using the four compounds mentioned above as possible topical antimicrobials against the clinical and reference strains of multidrug-resistant bacteria.


Asunto(s)
Antiinfecciosos , Dendrímeros , Staphylococcus aureus Resistente a Meticilina , Humanos , Dendrímeros/farmacología , Células HeLa , Antibacterianos/farmacología , Antiinfecciosos/farmacología , Biopelículas , Pruebas de Sensibilidad Microbiana
3.
Trop Med Int Health ; 28(12): 912-922, 2023 12.
Artículo en Inglés | MEDLINE | ID: mdl-37905331

RESUMEN

OBJECTIVE: The aim of this study is to assess Trypanosoma cruzi infection prevalence among pregnant migrants living in Madrid according to the country of origin and to assess screening coverage in this at-risk population. METHODS: Retrospective multicentre cross-sectional study conducted from January 2011 to December 2016 in eight Madrid hospitals. Each hospital reviewed their microbiology data records to assess the screening coverage and serological diagnosis in all pregnant women coming from endemic areas. RESULTS: From 2011 to 2016, 149,470 deliveries were attended at the eight hospitals, and 11,048 pregnant women were screened for Chagas disease. Most cases (93.5%) were in women from Bolivia, who also showed the highest prevalence (12.4%, 95% confidence interval: 9.9-15.0). Pooled prevalence amongst the screened women was 2.9% (95% CI: 1.8-4.1). Chagas disease screening coverage varied greatly between centres, with a pooled mean coverage of 47% (95% CI: 37%-57%; 73% [95% CI: 63%-82%] for those centres with universal screening vs. 10% [95% CI: 6%-15%] for those with a selective screening approach; p < 0.001). CONCLUSION: Our study provides useful data for policy makers and epidemiologists in a non-endemic area without congenital Chagas screening programmes.


Asunto(s)
Enfermedad de Chagas , Trypanosoma cruzi , Femenino , Humanos , Embarazo , Mujeres Embarazadas , Estudios Transversales , España/epidemiología , Prevalencia , América Latina/epidemiología , Transmisión Vertical de Enfermedad Infecciosa , Enfermedad de Chagas/diagnóstico
4.
Rev. esp. quimioter ; 35(6): 559-562, dic. 2022. tab
Artículo en Español | IBECS | ID: ibc-213140

RESUMEN

Introducción. Evaluar la implantación del Xpert-MTB/RIF®, como técnica de diagnóstico precoz, en una zona rural de Etiopía. Material y métodos. Se recogieron retrospectivamente los datos de aquellos pacientes mayores de 13 años a los que se solicitó la prueba Xpert MTB/RIF® en un hospital rural situado a 45 km del laboratorio de referencia, durante los 3 primeros años de su implantación (abril 2015-abril 2018). Resultados. Se evaluaron 306 pacientes mayores de 13 años, en 85 (27,8%) hubo un error en el procesamiento de la prueba y no se obtuvo el resultado. De las 221 muestras con resultados, la mediana de tiempo entre la obtención de la muestra y la recepción del resultado fue de 21 días y 42 de ellas fueron positivas (19%, IC 95%: 14,2-24,9%). La muestra de mayor rentabilidad diagnóstica fue la adenopatía (88,8%; [8/9];p<0.001). Conclusiones. Hay más diagnósticos bacteriológicos con Xpert-MTB/RIF®, pero con una tardanza en obtener el resultado y no se logra su principal objetivo que es diagnóstico precoz. (AU)


Introduction. To evaluate the implementation of Xpert-MTB/RIF®, as an early diagnosis technique, in a rural area of Ethiopia. Material and methods. Data were retrospectively collected from those patients over 13 years of age who were requested to take the Xpert MTB/RIF® test in a rural hospital located 45 km from the reference laboratory, during the first 3 years of its implementation (2015, April -2018, April). Results. A total of 306 patients older than 13 years were evaluated, in 85 (27.8%) there was an error in the processing of the test and the result was not obtained. Of the 221 samples with results, the median time between obtaining the sample and receiving the result was 21 days and 42 of them were positive (19%, 95% CI: 14.2-24.9%). The sample with the highest diagnostic yield was adenopathy (88.8%; [8/9]; p<0.001). Conclusions. There are more bacteriological diagnoses with Xpert-MTB/RIF®, but with a delay in obtaining the result and its main objective, which is early diagnosis, is not achieved. (AU)


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Tuberculosis/diagnóstico , Tuberculosis/epidemiología , Mycobacterium tuberculosis , Estudios Retrospectivos , Epidemiología Descriptiva , Hospitales Rurales , Etiopía/epidemiología
5.
Rev. esp. quimioter ; 35(5): 492-497, Oct. 2022. tab, graf
Artículo en Español | IBECS | ID: ibc-210703

RESUMEN

Introducción. La necesidad de integrar en la práctica clínica las resistencias locales es cada vez más urgente, especialmente en Atención Primaria, donde el tratamiento empírico es frecuente. Material y métodos. Se desarrolló un estudio retrospectivo observacional en el área de salud de Alcalá de Henares de los aislados microbiológicos positivos de Neisseria gonorrhoeae de cualquier localización (uretral, cervical, faríngea, rectal u orina). Se analizaron características sociodemográficas y resistencias a cefalosporinas, azitromicina, penicilina y quinolonas. Se relacionó cada aislado con su código postal de procedencia. Resultados. Se analizaron 256 muestras microbiológicas de N. gonorrhoeae, la mayoría pertenecientes a hombres (92,9%) con edad media de 33 años. La mitad de las muestras (49,8%) fueron resistentes a ciprofloxacino. La evolución temporo-espacial de las resistencias antimicrobianas se integró en mapas de calor con los códigos postales con más resistencias. Conclusión. Conocer las resistencias locales puede ayudar a pautar tratamientos empíricos más adecuados, especialmente en Atención Primaria, evitando la utilización de antibióticos inadecuados y disminuyendo las tasas de resistencias. (AU)


Introduction. The need to integrate local resistances into clinical practice is increasingly urgent, especially in Primary Care where empirical treatment is frequent. Methods. A retrospective observational study of positive microbiological isolates of Neisseria gonorrhoeae from any location (urethral, cervical, pharyngeal, rectal or urine) was carried out in the health area of Alcalá de Henares. Sociodemographic characteristics and resistance to cephalosporins, azithromycin, penicillin and quinolones were analyzed. Each isolate was related to its postal code of origin. Results. We analyzed 256 microbiological samples of N.gonorrhoeae, most of them male (92.9%) with a mean age of 33 years. Half of the samples (49.8%) were resistant to ciprofloxacin. Temporal and spatial evolution of antimicrobial resistance was integrated in heat maps. Conclusion. Knowing local resistances can help to prescribe more adequate empirical treatments, especially in Primary Care, avoiding inadequate antibiotics and decreasing resistance rates. (AU)


Asunto(s)
Humanos , Masculino , Femenino , Adulto Joven , Adulto , Persona de Mediana Edad , Procesamiento Automatizado de Datos , Neisseria gonorrhoeae , Resistencia a Medicamentos , Estudios Retrospectivos , Atención Primaria de Salud , Antibacterianos
6.
Brain Sci ; 12(7)2022 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-35884684

RESUMEN

Symptoms of Attention Deficit Hyperactivity Disorder (ADHD) include excessive activity, difficulty sustaining attention, and inability to act in a reflective manner. Early diagnosis and treatment of ADHD is key but may be influenced by the observation and communication skills of caregivers, and the experience of the medical professional. Attempts to obtain additional measures to support the medical diagnosis, such as reaction time when performing a task, can be found in the literature. We propose an information recording system that allows to study in detail the behavior shown by children already diagnosed with ADHD during a car driving video game. We continuously record the participants' activity throughout the task and calculate the error committed. Studying the trajectory graphs, some children showed uniform patterns, others lost attention from one point onwards, and others alternated attention/inattention intervals. Results show a dependence between the age of the children and their performance. Moreover, by analyzing the positions by age over time using clustering, we show that it is possible to classify children according to their performance. Future studies will examine whether this detailed information about each child's performance pattern can be used to fine-tune treatment.

7.
Antibiotics (Basel) ; 10(12)2021 Dec 03.
Artículo en Inglés | MEDLINE | ID: mdl-34943693

RESUMEN

Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.

8.
J Clin Virol ; 133: 104659, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-33160179

RESUMEN

BACKGROUND: RT-qPCR is the current recommended laboratory method to diagnose SARS-CoV-2 acute infection, several factors such as requirement of special equipment, time consuming, high cost and skilled staff limit the use of these techniques. A more rapid and high-throughput method is essential. METHODS: We analyzed clinical data and nasopharyngeal samples, collected during September 2020, from patients attended at the emergency department of a secondary hospital and in two primary healthcare centers in Madrid. The performance of the Panbio™ COVID-19 AG Rapid Test Device for the detection of SARS-CoV-2 antigen was compared to RT-qPCR. RESULTS: 255 nasopharyngeal swabs, including 150 from the emergency department and 105 from primary helthcare centers, were tested. 184 patients were symptomatic (72.1 %). Amongst the 60 positive RT-qPCR samples, 40 were detected by the rapid antigen test, given an overall sensitivity of 73.3 %. All the samples detected positive with the rapid antigen test were also positive with RT-qPCR. The median cycle threshold was 23.28 (IQR 18.5-30.16). Patients with less than seven days onset of symptoms showed a higher viral load, and sensitivity for rapid antigen test (86.5 %), compared to those with more days (sensitivity of 53.8 %)(p < 0.004). CONCLUSIONS: The rapid antigen test evaluated in this study showed a high sensitivity and specificity in samples obtained during the first week of symptoms and with high viral loads. This assay seems to be an effective strategy for controlling the COVID-19 pandemic for the rapid identification and isolation of SARS-CoV-2 infected patients.


Asunto(s)
Antígenos Virales/análisis , Prueba de COVID-19/métodos , COVID-19/diagnóstico , Adulto , Anciano , COVID-19/inmunología , Diagnóstico Precoz , Femenino , Humanos , Inmunoensayo , Masculino , Persona de Mediana Edad , Nasofaringe/virología , Pruebas en el Punto de Atención , Juego de Reactivos para Diagnóstico , Sensibilidad y Especificidad , Carga Viral
9.
Brain Sci ; 10(10)2020 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-33050130

RESUMEN

In the last few years, several computerized tasks have been developed to increase the objectivity of the diagnosis of attention-deficit hyperactivity disorder (ADHD). This article proposes the "running raccoon" video game to assess the severity of inattention in patients diagnosed with ADHD. Unlike existing tests, the proposed tool is a genuine video game in which the patient must make a raccoon avatar jump to avoid falling into different gaps. The distance to the gap is recorded for each jump. To evaluate the proposed game, an experiment was conducted in which 32 children diagnosed with ADHD participated. For each participant, the median and interquartile range of these distances were calculated, along with the number of omissions. Experimental results showed a significant correlation between the participants' inattention (measured by the Attention-Deficit/Hyperactivity Disorder Symptoms and Normal Behavior rating scale (SWAN) inattention subscale) with each of these three measures. In addition to its accuracy, other benefits are its short duration and the possibility of being run on both standard computers and mobile devices. These characteristics facilitate its acceptance in clinical environments or even its telematic use. The obtained results, together with the characteristics of the video game, make it an excellent tool to support clinicians in the diagnosis of ADHD.

10.
Salud Colect ; 16: e2553, 2020 May 26.
Artículo en Español | MEDLINE | ID: mdl-32574465

RESUMEN

This ethnographic study was designed to explore living conditions among a group of aboriginal families residing in an urban context, who participated in a recreational social program. The aim of the study was to explore life satisfaction by analyzing their living conditions and cultural capital inscribed in established attitudes, perceptions, and lay knowledge. Fieldwork was carried out in two complementary phases: phase 1, between May 2008 and December 2010, began when the first listed author initiated volunteer work with the organization responsible for the program; in phase 2, which extended from January 2011 to February 2013, participant observation was intensified and semi-structured interviews were carried out. The results indicate that the process of cultural uprooting underscores the experience of social inequality, and suggest a cohort effect that continues to this day. This process of cultural (in)consonance must be taken into account as a key factor when analyzing the living conditions and well-being of ethnic minorities, as well as when developing programs and interventions.


Este estudio etnográfico fue diseñado para explorar las condiciones de vida de un grupo de familias aborígenes residentes en un contexto urbano, beneficiarias de un programa sociorecreativo en Sídney, Australia. El objetivo fue explorar la satisfacción vital mediante el análisis de sus condiciones de vida y del capital cultural inscrito en sus actitudes, percepciones y conocimientos establecidos. El trabajo de campo se estructuró en dos fases complementarias: en la fase 1, entre mayo de 2008 y diciembre de 2010, se inició con el ingreso del primer autor como voluntario en la asociación responsable del programa y, en la fase 2, entre enero de 2011 y febrero de 2013 se intensificó el proceso de observación participante y las entrevistas semiestructuradas. Los resultados muestran que el proceso de desarraigo cultural está en la base de las experiencias vitales de desigualdad social experimentadas y apunta a un efecto de cohorte que llega hasta nuestros días. Dicho proceso de (des)consonancia cultural debe ser tenido en cuenta como un factor clave a la hora de analizar las condiciones de vida y bienestar de estas minorías étnicas, así como a la hora de desarrollar programas e intervenciones.


Asunto(s)
Aculturación , Nativos de Hawái y Otras Islas del Pacífico/etnología , Satisfacción Personal , Condiciones Sociales , Población Urbana , Actividades Cotidianas , Antropología Cultural , Australia/etnología , Relaciones Comunidad-Institución , Femenino , Humanos , Estilo de Vida , Masculino , Autonomía Personal , Evaluación de Programas y Proyectos de Salud , Investigación Cualitativa , Calidad de Vida , Determinantes Sociales de la Salud , Discriminación Social , Predominio Social , Factores Socioeconómicos
11.
Artículo en Inglés | MEDLINE | ID: mdl-32192115

RESUMEN

Emergency nurses are exposed to traumatic events and routine stressors, both of which can lead to the development of PTSD (Post Traumatic Stress Disorder) symptomatology. However, there are currently no instruments designed to assess the impact and frequency of such sources of stress in nurses. The Traumatic and Routine Stressors Scale on Emergency Nurses (TRSS-EN) was built for this purpose. A sample of 147 emergency nurses from three hospitals in Madrid (Spain) completed this 13-item scale. The analyses showed a factorial structure composed of two factors. The first is characterized by items regarding traumatic and stressful events and procedures of severe magnitude (traumatic stressors), and the second by items related to stressful events and procedures of moderate magnitude (routine stressors) but hypothesized to possess a substantial traumatic potential. Analyses provided evidence of both adequate internal consistency (Cronbach's α = 0.92; first factor α = 0.91 and second factor α = 0.86) and test-retest reliability. In addition, concurrent validity also proved to be satisfactory. In short, TRSS-EN seems to be a reliable and valid tool in a healthcare emergency nursing setting for screening the frequency and impact of exposure to everyday work-related traumatic stressors, either event-related or routine.


Asunto(s)
Servicio de Urgencia en Hospital , Enfermeras y Enfermeros , Estrés Psicológico , Adulto , Femenino , Humanos , Masculino , Enfermeras y Enfermeros/psicología , Psicometría , Reproducibilidad de los Resultados , España , Encuestas y Cuestionarios , Heridas y Lesiones/psicología
12.
Salud colect ; 16: e2553, 2020.
Artículo en Español | LILACS | ID: biblio-1139504

RESUMEN

RESUMEN Este estudio etnográfico fue diseñado para explorar las condiciones de vida de un grupo de familias aborígenes residentes en un contexto urbano, beneficiarias de un programa sociorecreativo en Sídney, Australia. El objetivo fue explorar la satisfacción vital mediante el análisis de sus condiciones de vida y del capital cultural inscrito en sus actitudes, percepciones y conocimientos establecidos. El trabajo de campo se estructuró en dos fases complementarias: en la fase 1, entre mayo de 2008 y diciembre de 2010, se inició con el ingreso del primer autor como voluntario en la asociación responsable del programa y, en la fase 2, entre enero de 2011 y febrero de 2013 se intensificó el proceso de observación participante y las entrevistas semiestructuradas. Los resultados muestran que el proceso de desarraigo cultural está en la base de las experiencias vitales de desigualdad social experimentadas y apunta a un efecto de cohorte que llega hasta nuestros días. Dicho proceso de (des)consonancia cultural debe ser tenido en cuenta como un factor clave a la hora de analizar las condiciones de vida y bienestar de estas minorías étnicas, así como a la hora de desarrollar programas e intervenciones.


ABSTRACT This ethnographic study was designed to explore living conditions among a group of aboriginal families residing in an urban context, who participated in a recreational social program. The aim of the study was to explore life satisfaction by analyzing their living conditions and cultural capital inscribed in established attitudes, perceptions, and lay knowledge. Fieldwork was carried out in two complementary phases: phase 1, between May 2008 and December 2010, began when the first listed author initiated volunteer work with the organization responsible for the program; in phase 2, which extended from January 2011 to February 2013, participant observation was intensified and semi-structured interviews were carried out. The results indicate that the process of cultural uprooting underscores the experience of social inequality, and suggest a cohort effect that continues to this day. This process of cultural (in)consonance must be taken into account as a key factor when analyzing the living conditions and well-being of ethnic minorities, as well as when developing programs and interventions.


Asunto(s)
Humanos , Masculino , Femenino , Satisfacción Personal , Condiciones Sociales , Población Urbana , Aculturación , Calidad de Vida , Australia/etnología , Predominio Social , Factores Socioeconómicos , Actividades Cotidianas , Evaluación de Programas y Proyectos de Salud , Relaciones Comunidad-Institución , Autonomía Personal , Investigación Cualitativa , Discriminación Social , Determinantes Sociales de la Salud , Estilo de Vida , Antropología Cultural
13.
Malar J ; 18(1): 242, 2019 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-31315624

RESUMEN

BACKGROUND: The importance of submicroscopic malaria infections in high-transmission areas could contribute to maintain the parasite cycle. Regarding non-endemic areas, its importance remains barely understood because parasitaemia in these afebrile patients is usually below the detection limits for microscopy, hence molecular techniques are often needed for its diagnosis. In addition to this, the lack of standardized protocols for the screening of submicroscopic malaria in immigrants from endemic areas may underestimate the infection with Plasmodium spp. The aim of this study was to assess the prevalence of submicroscopic malaria in afebrile immigrants living in a non-endemic area. METHODS: A prospective, observational, multicentre study was conducted. Afebrile immigrants were included, microscopic observation of Giemsa-stained thin and thick blood smears, and two different molecular techniques detecting Plasmodium spp. were performed. Patients with submicroscopic malaria were defined as patients with negative blood smears and detection of DNA of Plasmodium spp. with one or both molecular techniques. Demographic, clinical, analytical and microbiological features were recorded and univariate analysis by subgroups was carried out with STATA v15. RESULTS: A total of 244 afebrile immigrants were included in the study. Of them, 14 had a submicroscopic malaria infection, yielding a prevalence of 5.7% (95% confidence interval 3.45-9.40). In 71.4% of the positive PCR/negative microscopy cases, Plasmodium falciparum alone was the main detected species (10 out of the 14 patients) and in 4 cases (28.6%) Plasmodium vivax or Plasmodium ovale were detected. One patient had a mixed infection including three different species. CONCLUSIONS: The prevalence of submicroscopic malaria in afebrile immigrants was similar to that previously described in Spain. Plasmodium vivax and P. ovale were detected in almost a third of the submicroscopic infections. Screening protocols for afebrile immigrants with molecular techniques could be useful for a proper management of these patients.


Asunto(s)
Enfermedades Asintomáticas/epidemiología , Malaria/epidemiología , Plasmodium falciparum/aislamiento & purificación , Plasmodium ovale/aislamiento & purificación , Plasmodium vivax/aislamiento & purificación , Adulto , Coinfección/epidemiología , Coinfección/parasitología , Emigrantes e Inmigrantes , Femenino , Humanos , Malaria/parasitología , Malaria Falciparum/epidemiología , Malaria Falciparum/parasitología , Malaria Vivax/epidemiología , Malaria Vivax/parasitología , Masculino , Microscopía , Persona de Mediana Edad , Prevalencia , España/epidemiología
14.
PLoS One ; 13(12): e0207822, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30533050

RESUMEN

Rapid diagnosis is one of the best ways to improve patient management and prognosis as well as to combat the development of bacterial resistance. The aim of this study was to study parameters that impact the achievement of reliable identification using a combination of flow cytometry and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS).The study was carried out in nine hospitals in Spain and included 1,050 urine samples with bacterial counts of 5x106 bacteria/ml. MALDI-ToF-MS-based identification was performed according to a previously described protocol. Valid identification by direct MALDI-ToF-MS was obtained in 72.8% of samples, in 80.3% of samples found to be positive by culture, 32.2% of contaminated samples, and 19.7% of negative samples. Among the positives samples with a valid identification the concordance at the species level was 97.2%. The parameters related to success of direct identification were: high bacterial count, the presence of Escherichia coli as a pathogen and rod-bacteria morphology provided by flow cytometry. The parameters related to failure were a high epithelial cell (EC) count, a high white blood cell (WBC) count and urine samples obtained from in-patients. In summary, this multicentre study confirms previously published data on the usefulness and accuracy of direct MALDI-ToF-MS-based identification of bacteria from urine samples. It seems important to evaluate not only the bacterial count, but also other parameters, such as EC and WBC counts, bacterial species and morphology, and the health care setting, to decide whether the sample is suitable for direct identification.


Asunto(s)
Bacteriuria/diagnóstico , Bacteriuria/microbiología , Infecciones Urinarias/diagnóstico , Infecciones Urinarias/microbiología , Orina/microbiología , Adulto , Anciano , Anciano de 80 o más Años , Técnicas Bacteriológicas , Infecciones por Escherichia coli/diagnóstico , Infecciones por Escherichia coli/microbiología , Femenino , Citometría de Flujo , Humanos , Masculino , Persona de Mediana Edad , Reproducibilidad de los Resultados , España , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
15.
Artículo en Inglés | MEDLINE | ID: mdl-30231166

RESUMEN

Tinea nigra is an infrequent, superficial fungal infection, mainly caused by Hortaea werneckii, which is still underreported in Ethiopia. An asymptomatic 62-year-old male patient sought a rural hospital of Ethiopia, showing dark plaques on the palms of both hands. A superficial mycosis was suspected and a direct light microscopic mycological examination from skin scrapings revealed short brownish hyphae. To our knowledge, this is the first case of tinea nigra from the Ethiopian highlands. This may be due to the actual rarity of the condition or to underreporting.


Asunto(s)
Dermatosis de la Mano/diagnóstico , Tiña/diagnóstico , Antifúngicos/uso terapéutico , Dermatosis de la Mano/tratamiento farmacológico , Dermatosis de la Mano/microbiología , Humanos , Masculino , Miconazol/análogos & derivados , Miconazol/uso terapéutico , Persona de Mediana Edad , Servicios de Salud Rural , Tiña/tratamiento farmacológico
16.
Malar J ; 17(1): 314, 2018 Aug 29.
Artículo en Inglés | MEDLINE | ID: mdl-30157862

RESUMEN

BACKGROUND: Malaria is currently the most important human parasitic disease in the world responsible for high morbidity and mortality. Appropriate diagnostic methods are essential for early detection. Microscopy examination remains the gold standard, although molecular techniques have higher sensitivity and are very useful in cases of low parasitaemia and mixed infections. The objective of this study was to evaluate a new commercial molecular diagnostic technique. METHODS: A prospective, observational, multicentre study was performed between January 2015 and April 2017. All participants were immigrants from malaria-endemic areas, who were divided into two groups: asymptomatic group and symptomatic. Samples from both groups were evaluated by a rapid diagnostic test (ImmunoQuick® Malaria + 4 RDT), microscopy examination, and two commercial molecular malaria tests (FTD Malaria and FTD Malaria Differentiation), then compared against an in-house reference PCR technique. RESULTS: In all, 250 patients were included: 164 (65.6%) in the asymptomatic group, and 86 (34.4%) in the symptomatic group. There were seven cases of asymptomatic parasitaemia (prevalence = 2.8%) that were detected only by molecular methods. In the symptomatic group, there were seven cases of submicroscopic malaria. The main species detected was Plasmodium falciparum (96.6%). The commercial molecular technique had higher sensitivity than the other methods (S = 96%) and a high rate of concordance with the in-house reference PCR technique (Kappa score = 0.93). CONCLUSIONS: The molecular techniques, although slower than microscopy, have adequate diagnostic accuracy and are very useful for the detection of P. falciparum in cases with low parasitaemia.


Asunto(s)
Pruebas Diagnósticas de Rutina/métodos , Malaria Falciparum/epidemiología , Malaria Vivax/epidemiología , Malaria/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Parasitemia/epidemiología , Adulto , Anciano , Anciano de 80 o más Años , Ciudades , Emigrantes e Inmigrantes , Humanos , Malaria Falciparum/parasitología , Malaria Vivax/parasitología , Persona de Mediana Edad , Parasitemia/parasitología , Plasmodium falciparum/aislamiento & purificación , Plasmodium vivax/aislamiento & purificación , Valor Predictivo de las Pruebas , Prevalencia , Estudios Prospectivos , España/epidemiología , Adulto Joven
17.
Rev. bras. ciênc. esporte ; 40(2): 177-183, abr.-jun. 2018.
Artículo en Español | LILACS | ID: biblio-958456

RESUMEN

Resumen Este estudio analiza la percepción del profesorado para salvaguardar los juegos y deportes tradicionales en Bali. Se realizó un estudio cualitativo, a través de grupos de discusión y entrevistas, con la participación de treinta y dos docentes. Los resultados indican que el profesorado es el principal recolector y protector de los juegos. Los modifica y adapta funcional y estructuralmente, para que adquieran rango educativo y los considera como parte esencial de la identidad balinesa, por lo que considera que se debería de profundizar en la enseñanza de la lógica externa del juego. También se percibe la carencia de regulación de estos en el currículo oficial, la falta de promoción a nivel regional y nacional, así como la necesidad de incluirlos en los programas universitarios, documentarlos e inventariarlos.


Abstract This study analyzes teachers' perceptions to safeguard traditional games and sports in Bali. A qualitative study was carried out through discussion groups and interviews with the participation of thirty-two teachers. The results indicate that teachers are the main collector and protector of games. Teachers modify and adapt them functionally and structurally, so that they acquire educational rank and considers them as an essential part of the Balinese identity, reason why considers that one should deepen in the teaching of the external logic of the game. Also, it is perceived the lack of regulation of these in the official curriculum, the lack of promotion at regional and national level, as well as the need to include them in university programs, document and inventory them.


Resumo Este estudo analisa as perceções dos professores para salvaguardar os jogos e os desportos tradicionais em Bali. Foi feito um estudo qualitativo, por meio de grupos de discussão e entrevistas, com a participação de 32 professores. Os resultados indicam que os professores são o principal colecionador e protetor de jogos. Modificam e adaptam os jogos de forma funcional e estrutural, para que adquiram uma classificação educacional e os considerem uma parte essencial da identidade balinesa, por isso consideram que se deve aprofundar o ensino da lógica externa do jogo. Também se percebe a falta de regulação desses no currículo oficial, a falta de promoção em nível regional e nacional, bem como a necessidade de incluí-los em programas universitários, documentá-los e inventariá-los.

18.
Med Mycol Case Rep ; 19: 21-24, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29234586

RESUMEN

Keratitis produced by Aspergillus tamarii has been previously described associated to an ocular injury. We report a case in a contact lens wearer with a history of previous bilateral myopic LASIK ablation, bilateral intracorneal rings and vitrectomy and scleral buckling in his left eye. The fungus could be quickly identified combining phenotype, microscopy and mass spectrometry. Treatment with intravenous amphotericin, oral voriconazole, and topical amphotericin and natamycin and voriconazole was needed for corneal preservation.

19.
Ann Agric Environ Med ; 24(4): 712-715, 2017 Dec 23.
Artículo en Inglés | MEDLINE | ID: mdl-29284252

RESUMEN

INTRODUCTION: Cutaneous anthrax is a zoonotic disease caused by the spore-forming bacterium Bacillus anthracis, which typically presents with ulcers after contact with animals or animal products, and is rarely seen in high-income countries but is common in those with low- and middle-incomes. Objective. The aim of this study is to show the main clinical characteristics of cutaneous anthrax in endemic areas. MATERIAL AND METHODS: The study describes the main clinical characteristics of cutaneous anthrax in eight patients (six female and two male, age range 1 - 56 years) admitted to the rural General Hospital of Gambo, West Arsi Province of Ethiopia from 2010-2013. RESULTS: In all cases, lesions began as an erythematous papule located on exposed sites (n=7 head; n=1 thigh) and subsequently became a necrotic black eschar surrounded by an edematous halo. Two patients presented with painful ipsilateral adenopathy near the black eschar. Four patients developed a malignant pustule on the suborbital region of the face. Patients responded positively to treatment, and the lesions resolved, leaving eschars. However, one patient suffered the loss of an eyeball, and another died 12 hours after starting treatment. CONCLUSIONS: Physicians working in rural areas of resource-poor settings should be trained in the clinical identification of cutaneous anthrax. Early antibiotic treatment is essential for decreasing morbidity and mortality.


Asunto(s)
Carbunco/diagnóstico , Bacillus anthracis/fisiología , Enfermedades Cutáneas Bacterianas/diagnóstico , Adolescente , Adulto , Carbunco/tratamiento farmacológico , Carbunco/epidemiología , Carbunco/microbiología , Antibacterianos/administración & dosificación , Bacillus anthracis/efectos de los fármacos , Bacillus anthracis/genética , Bacillus anthracis/aislamiento & purificación , Niño , Preescolar , Etiopía/epidemiología , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Población Rural , Enfermedades Cutáneas Bacterianas/tratamiento farmacológico , Enfermedades Cutáneas Bacterianas/epidemiología , Enfermedades Cutáneas Bacterianas/microbiología , Adulto Joven
20.
Univ. psychol ; 16(4): 246-258, oct.-dic. 2017. graf, tab
Artículo en Español | LILACS, COLNAL | ID: biblio-963318

RESUMEN

Resumen En este trabajo se analiza la relación entre la satisfacción con la imagen corporal, la autoestima y el autoconcepto físico. Los participantes fueron 310 alumnos, de 5º y 6º de Educación Primaria, y 22 profesores en activo, de la zona educativa Motril-Costa (España). Como técnicas cuantitativas, se utilizaron: el Cuestionario de Siluetas Corporales, Cuestionario de Autoconcepto Físico y la Escala de Autoestima de Rosenberg. Como técnica cualitativa, se utilizó la entrevista semi-estrucutrada. Los resultados muestran que difiere la imagen que tienen y que les gustaría tener. También se aprecia que los chicos tienen un mayor nivel de autoestima. Son las chicas las que se consideran menos hábiles para realizar actividad física y disfrutan en menor medida de estas actividades.


Abstract In this paperis analyzed the relationshipbetween satisfaction withbody image,self-esteem andphysical self.Participants were310students,grades 5 and 6primary and22practicing teachers,the educationalareaMotril-Costa (Spain). Asquantitative techniquesQuestionnairebody silhouettes, Physical SelfQuestionnaire and theRosenbergSelf-Esteem Scale.Asthe qualitative technique was used semi-structured interview. The results show thatdifferent fromthe image they haveand would like tohave.It is also appreciatedthat the boyshave a higher levelofself-esteem.It isthe girls whoare consideredless abletoenjoyphysical activityandto a lesser extentof these activities.


Asunto(s)
Autoimagen , Imagen Corporal/psicología , Adolescente
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