RESUMEN
AIM: We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools. CONCLUSION: Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
Asunto(s)
Anticuerpos Monoclonales/análisis , Técnicas Biosensibles/métodos , Inmunoensayo/métodos , Imanes/química , Nanopartículas/química , Ovalbúmina/análisis , Técnicas Biosensibles/instrumentación , Colorimetría/métodos , Ensayo de Inmunoadsorción Enzimática , Inmunoensayo/instrumentación , Límite de Detección , Campos Magnéticos , Ovalbúmina/inmunologíaRESUMEN
Faraday polarization rotators are commonly used in laser experiments. Most Faraday materials have a nonnegligible absorption, which is a limiting factor for high power laser optical isolators or for intracavity optical diodes. By using a stronger magnetic field and a shorter length of Faraday material, one can obtain the same polarization rotation and a reduced absorption. In this paper, we describe two permanent magnet arrangements that are easy to build and produce magnetic fields up to 1.7 T, substantially more than commonly used. The field homogeneity is largely sufficient for a 30 dB isolation ratio. We finally discuss the prospects for producing even larger fields with permanent magnets.
