RESUMEN
BACKGROUND: Primary pancreatic lymphoma (PPL) is an exceedingly rare tumor with limited mention in scientific literature. The clinical manifestations of PPL are often nonspecific, making it challenging to distinguish this disease from other pancreatic-related diseases. Chemotherapy remains the primary treatment for these individuals. CASE SUMMARY: In this case study, we present the clinical details of a 62-year-old woman who initially presented with vomiting, abdominal pain, and dorsal pain. On further evaluation through positron emission tomography-computed tomography, the patient was considered to have a pancreatic head mass. However, subsequent endoscopic ultrasonography-guided fine needle aspiration (EUS-FNA) revealed that the patient had pancreatic peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS). There was a substantial decrease in the size of the pancreatic mass after the patient underwent a cycle of chemotherapy comprised of brentuximab vedotin, decitabine, and oxaliplatin (brentuximab vedotin and Gemox). The patient had significant improvement in radiological findings at the end of the first cycle. CONCLUSION: Primary pancreatic PTCL-NOS is a malignant and heterogeneous lymphoma, in which the clinical manifestations are often nonspecific. It is difficult to diagnose, and the prognosis is poor. Imaging can only be used for auxiliary diagnosis of other diseases. With the help of immunostaining, EUS-FNA could be used to aid in the diagnosis of PPL. After a clear diagnosis, chemotherapy is still the first-line treatment for such patients, and surgical resection is not recommended. A large number of recent studies have shown that the CD30 antibody drug has potential as a therapy for several types of lymphoma. However, identifying new CD30-targeted therapies for different types of lymphoma is urgently needed. In the future, further research on antitumor therapy should be carried out to improve the survival prognosis of such patients.
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The circulating tumor DNA (ctDNA) is a crucial cancer marker, its sensitive monitoring is useful for early diagnose and therapy of tumor-related diseases. Herein, a bipedal DNA walker with multiple recognition sites is designed through the transition of dumbbell-shaped DNA nanostructure to realize the dual amplification of the signal and achieve ultrasensitive photoelectrochemical (PEC) detection of ctDNA. Initially, the ZnIn2S4@AuNPs is obtained by combining the drop coating method with electrodeposition method. When the target is present, the dumbbell-shaped DNA structure transforms into an annular bipedal DNA walker that can walk unrestrictedly on the modified electrode. After the cleavage endonuclease (Nb.BbvCI) was added to the sensing system, the ferrocene (Fc) on the substrate is released from the electrode surface, and the transfer efficiency of photogenerated electron-hole pairs is extremely improved, enabling the "signal on" testing of ctDNA. The detection limit of the prepared PEC sensor is 0.31 fM, and the recovery of actual samples varied between 96.8 and 103.6% with an average relative standard deviation of about 8%. Meaningfully, the prepared PEC biosensor with an innovative bipedal DNA walker has potential application value for ultrasensitive detection of other nucleic acid-related biomarker.
Asunto(s)
Técnicas Biosensibles , ADN Tumoral Circulante , Nanopartículas del Metal , Neoplasias , Humanos , ADN Circular , Oro/química , Nanopartículas del Metal/química , Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Límite de Detección , ADN/química , Neoplasias/diagnósticoRESUMEN
Retinoic acid inducible gene I (RIGI) is upregulated during alltrans retinoic acid (ATRA)induced terminal granulocytic differentiation of NB4 acute promyelocytic leukemia (APL) cells. However, the function and mechanism of RIGI in NB4 cells remains to be fully elucidated. In the present study, lentivirusmediated RIGIknockdown was used to investigate the proliferation, cell cycle and apoptotic processes of ATRAinduced NB4 cells in vitro using an MTT assay and flow cytometry, respectively. The roles of RIGI and the AKTFOXO3A signaling pathway were investigated using western blot analysis. The results showed that the ATRAinduced expression of RIGI was specifically and effectively knocked down at the mRNA and protein levels by lentivirus mediated RIGI short hairpin RNA. In addition, silencing of RIGI reduced the ATRAinduced inhibition of NB4 cell proliferation, cell cycle arrest and apoptosis. Further investigations indicated that with ATRAinduced expression of RIGI, levels of phosphorylated (p)AKTThr308 and pForkhead Box (FOX) O3AThr32 were decreased, the expression levels of cell cycle arrest protein p27 and the apoptotic protein, tumor necrosis factorrelated apoptosisinducing ligand (TRAIL), directly transcribed by FOXO3A were increased. By contrast, following the knockdown of ATRAinduced expression of RIGI, the levels of pAKTThr308 and pFOXO3AThr32 were increased, and the protein expression levels of p27 and TRAIL were decreased. Taken together, these results showed that the knockdown of RIGI reduced the inhibition of cell proliferation, cell cycle arrest and apoptosis in the ATRAinduced NB4 cells via the AKTFOXO3A signaling pathway.