RESUMEN
BACKGROUND: Vincristine is a commonly used chemotherapeutic agent. It is associated with undesirable digestive side effects. However, the impact of vincristine on gastrointestinal structure and motility or its long-term effects have not been deeply studied in animal models. This could be useful in order to develop therapeutic or preventive strategies for cancer patients. The aim of this study was to analyze such effects. METHODS: Rats received saline or vincristine (0.1 mg kg-1 , ip) daily for 10 days. Evaluations were performed during treatment and 2-6 weeks after. Somatic mechano-sensitivity was assessed using von Frey hairs. Gastrointestinal motor function was studied by means of radiographic still images and colonic propulsion of fecal pellets using fluoroscopy videos. Histological assessment of the gut morphology and immunohistochemistry for HuC/D and nNOS were performed in whole-mount myenteric plexus preparations. KEY RESULTS: Peripheral sensitivity was increased in animals treated with vincristine and did not subside 2 weeks after treatment finalization. Vincristine treatment inhibited gastrointestinal motility although this was recovered to normal values with time. Damage in the digestive wall after vincristine treatment was greater in the ileum than in the colon. Villi shortening (in ileum) and large inflammatory nodules still remained 2 weeks after treatment finalization. Finally, the proportion of nNOS-immunoreactive neurons was increased with vincristine and continued to be increased 2 weeks after treatment finalization. CONCLUSIONS AND INFERENCES: Vincristine alters gastrointestinal motility, peripheral sensitivity and mucosal architecture. Vincristine-induced neuropathy (somatic and enteric), intestinal mucosa damage and inflammatory infiltrations are relatively long-lasting.
Asunto(s)
Antineoplásicos Fitogénicos/toxicidad , Motilidad Gastrointestinal/efectos de los fármacos , Tracto Gastrointestinal/efectos de los fármacos , Mucosa Intestinal/efectos de los fármacos , Vincristina/toxicidad , Animales , Masculino , Ratas , Ratas WistarRESUMEN
No disponible
Asunto(s)
Animales , Ratas , Trastornos de la Motilidad Esofágica/tratamiento farmacológico , Trastornos de la Motilidad Esofágica/veterinaria , Morfina/uso terapéutico , Fluoroscopía , Sulfato de Bario/uso terapéutico , Congresos como Asunto , Fluoroscopía/veterinaria , Modelos Animales , Inyecciones Intraperitoneales , Inyecciones Intraperitoneales/veterinaria , Análisis de Varianza , ColonRESUMEN
No disponible
Asunto(s)
Humanos , Máscaras Laríngeas , Miastenia Gravis , Anestesia , AbdomenRESUMEN
No disponible