Somatic mutational profiling and clinical impact of driver genes in Latin-Iberian medulloblastomas: Towards precision medicine.

Medulloblastoma (MB) is the most prevalent malignant brain tumor in children, known for its heterogeneity and treatment-associated toxicity, and there is a critical need for new therapeutic targets. We analyzed the somatic mutation profile of 15 driver genes in 69 Latin-Iberian molecularly characterized medulloblastomas using the Illumina TruSight Tumor 15 panel. We classified the variants based on their clinical impact and oncogenicity. Among the patients, 66.7% were MBSHH, 13.0% MBWNT, 7.3% MBGrp3, and 13.0% MBGrp4. Among the 63 variants found, 54% were classified as Tier I/II and 31.7% as oncogenic/likely oncogenic. We observed 33.3% of cases harboring at least one mutation. TP53 (23.2%, 16/69) was the most mutated gene, followed by PIK3CA (5.8%, 4/69), KIT (4.3%, 3/69), PDGFRA (2.9%, 2/69), EGFR (1.4%, 1/69), ERBB2 (1.4%, 1/69), and NRAS (1.4%, 1/69). Approximately 41% of MBSHH tumors exhibited mutations, TP53 (32.6%) being the most frequently mutated gene. Tier I/II and oncogenic/likely oncogenic TP53 variants were associated with relapse, progression, and lower survival rates. Potentially actionable variants in the PIK3CA and KIT genes were identified. Latin-Iberian medulloblastomas, particularly the MBSHH, exhibit higher mutation frequencies than other populations. We corroborate the TP53 mutation status as an important prognostic factor, while PIK3CA and KIT are potential therapeutic targets.

Essential oil and fenchone extracted from Tetradenia riparia (Hochstetter.) Codd (Lamiaceae) induce oxidative stress in Culex quinquefasciatus larvae (Diptera: Culicidae) without causing lethal effects on non-target animals.

We investigated the larvicidal activity of the essential oil (EO) from Tetradenia riparia and its majority compound fenchone for controlling Culex quinquefasciatus larvae, focusing on reactive oxygen and nitrogen species (RONS), catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) activities, and total thiol content as oxidative stress indicators. Moreover, the lethal effect of EO and fenchone was evaluated against Anisops bouvieri, Diplonychus indicus, Danio rerio, and Paracheirodon axelrodi. The EO and fenchone (5 to 25 µg/mL) showed larvicidal activity (LC50 from 16.05 to 18.94 µg/mL), followed by an overproduction of RONS, and changes in the activity of CAT, GST, AChE, and total thiol content. The Kaplan-Meier followed by Log-rank (Mantel-Cox) analyses showed a 100% survival rate for A. bouvieri, D. indicus, D. rerio, and P. axelrodi when exposed to EO and fenchone (262.6 and 302.60 µg/mL), while α-cypermethrin (0.25 µg/mL) was extremely toxic to these non-target animals, causing 100% of death. These findings emphasize that the EO from T. riparia and fenchone serve as suitable larvicides for controlling C. quinquefasciatus larvae, without imposing lethal effects on the non-target animals investigated.

High frequency of WNT-activated medulloblastomas with CTNNB1 wild type suggests a higher proportion of hereditary cases in a Latin-Iberian population.

Purpose: Medulloblastomas are the most common primary malignant brain tumors in children. They are divided into molecular subgroups: WNT-activated, SHH-Activated, TP53 mutant or wild type, and non-WNT/non-SHH (Groups 3 and 4). WNT-activated medulloblastomas are usually caused by mutations in the CTNNB1 gene (85%-90%), and most remaining cases of CTNNB1 wild type are thought to be caused by germline mutations in APC. So far, the frequencies of CTNNB1 have been reported mainly in North American and European populations. The aim of this study was to report the frequency of CTNNB1 mutations in WNT-activated medulloblastomas in a Latin-Iberian population and correlate with their clinicopathological characteristics. Methods: A total of 266 medulloblastomas from seven different institutions from Brazil (n=211), Portugal (n=38), and Argentina (n=17) were evaluated. Following RNA and DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tumor tissues, the molecular classification and CTNNB1 mutation analysis were performed by nCounter and Sanger sequencing, respectively. Results: WNT-activated medulloblastomas accounted for 15% (40/266) of the series. We observed that 73% of WNT-activated medulloblastomas harbored CTNNB1 mutations. CTNNB1 wild-type cases (27%) were more prevalent in female individuals and suggested to be associated with a worse outcome. Among the CTNNB1 wild-type cases, the available analysis of family history revealed two cases with familiar adenomatous polyposis, harboring APC germline variants. Conclusion: We observed a lower incidence of CTNNB1 mutations in WNT-activated medulloblastomas in our Latin-Iberian cohort compared to frequencies previously described in other populations. Considering that CTNNB1 wild-type cases may exhibit APC germline mutations, our study suggests a higher incidence (~30%) of hereditary WNT-activated medulloblastomas in the Latin-Iberian population.

Occurrence, Biological and Enzymatic Activities of Agaricomycetes from the Brazilian Amazon - A Systematic Review.

Agaricomycetes are highlighted for producing a variety of compounds and enzymes with nutritional and medicinal properties. However, the knowledge of the diversity of this group of fungi is still insufficient, as well as their biological and enzymatic activities. Thus, the objective of this work is to describe the occurrence, biological and enzymatic activities of Agaricomycetes from the Brazilian Amazon. The present study is a systematic review with the literature search done in the following databases: Scielo, Google Scholar, PubMed and ScienceDirect. The descriptors used were Basidiomycota, Agaricomycetes, mushroom, antimicrobial activity, antitumor activity, antioxidant activity, anti-inflammatory activity, immunomodulator, enzymatic activity, and Brazilian Amazon. We used as inclusion criteria articles in Portuguese and English, published between 2010 and 2021 and that had the full text available and presented relevance to the exposed topic, and as exclusion criteria, works not done in the Brazilian Amazon, duplicate articles in the databases search or outside the topic under study. A total of 40 articles, published between 2010 and 2021, were selected for analysis. 230 species of Agaricomycetes fungi were described for the Brazilian Amazon, with the most frequent orders being Polyporales (52.60%), Agaricales (14.35%), and Hymenochaetales (13.91%). Six studies were found on antimicrobial activity for promising Agaricomycete fungi against the bacteria Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and the fungi Candida albicans, C. parapsilosis and C. tropicalis. For the antioxidant activity, a study described the species Lentinus citrinus with high amounts of antioxidant compounds. For enzymatic activity, five studies reported Agaricomycete fungi producing protease, cellulase, amylase, pectinase, laccase, and xylanase enzymes. This review shows the scarcity of studies on the description and technological potential of Agaricomycetes from the Brazilian Amazon, highlighting the need to encourage the study of this group of organisms.

Tropical grass silages with spineless cactus in diets of Holstein × Zebu heifers in the semiarid region of Brazil.

One of the factors that limit ruminant production in the semiarid region is the lack of roughage in the dry season. The management of forage plants adapted to edaphoclimatic conditions is a strategy to improve animal production. This study was conducted to examine the effects of biomass sorghum silage (BSS; Sorghum bicolor (L.) Moench) and BRS capiaçu grass silage (CGS; Pennisetum purpureum Schum) with or without spineless cactus (Opuntia spp.) in crossbred Holstein × Zebu heifers' diets on the intake, apparent digestibility of the nutrients and animal performance (e.g., final weight, daily weight gain) (experiment 1). Also, to evaluate the ruminal kinetics of dry matter (DM) and neutral detergent fiber (NDF) of roughages used in diets using two animals cannulated in the rumen (experiment 2). In experiment 1, ten heifers with an initial body weight of 200 ± 2.74 kg (mean ± standard deviation) and a mean age of 10 months were used. The animals were distributed in an experimental design in two simultaneous 5 × 5 Latin squares. Five experimental diets were used: diet 1, Volumax sorghum silage (VSS); diet 2, biomass sorghum silage (BSS); diet 3, BRS capiaçu silage (CGS); diet 4, biomass sorghum silage (60%) with spineless cactus (40%) (BSS + SC); and diet 5, BRS capiaçu grass silage (60%) with spineless cactus (40%) (CGS + SC). The diets were formulated with sorghum silage or BRS capiaçu grass silage with or without spineless cactus (roughage) and a maize- and soybean-based concentrate (75:25 roughage-to-concentrate ratio) on DM basis. The experiment lasted 105 days, divided into five periods of 21 days (17 days for the adaptation of the animals to the diets and management and 4 for data collection and samples). The diets containing CGS and CGS + SC resulted in lower dry matter intake (DMI; 5.61 kg day-1; P < 0.01), which was 19.4% lower than the diets with VSS, BSS, and BSS + SC (7.00 kg day-1). The BSS + SC and CGS + SC diets showed higher crude protein digestibility (P < 0.01) at 21.9% than the other treatments (Volumax, BSS, CGS). The different diets did not change the final weight or the daily weight gain of the heifers. The BRS 716 biomass sorghum silage and BRS capiaçu grass combined with spineless cactus increased (P < 0.05) the intake of nonfibrous carbohydrates and did not interfere (P > 0.05) with the final weight or average daily gain of the crossbred Holstein × Zebu heifers. The standardized potentially degradable fraction (Bp) of the NDF was 13.91% higher (P < 0.01) for BSS and BSS + SC (61.6%) compared to the others (53.0%). A diet based on BSS + SC is recommended for feeding crossbred heifers in the growing phase.

How much time of sedentary behavior is associated with depressive symptoms in community-dwelling older adults in southern Brazil?

AIMS: Identify sedentary behavior (SB) cut-off points to screen for depressive symptoms in older adults and verify the association between these conditions. METHODS: A cross-sectional survey of 308 community-dwelling older adults was conducted. The outcome was the presence of depressive symptoms (≥6 points on the Geriatric Depression Scale-15). The exposure was SB using the self-reported time on a weekday and weekend (International Physical Activity Questionnaire). The cut-off points for SB categorization were determined by the receiver operating characteristic curve and multivariate logistic regression to verify the association. RESULTS: Older adults who spent ≥4.5 hours/day in SB (sensitivity = 48.8%; specificity = 67.8%) were 1.81 times more likely (95%CI: 1.03;3.15) to have depressive symptoms compared to those who stayed for shorter periods. CONCLUSIONS: There was an association between SB and depressive symptoms; therefore, older adults must have SB <4.5 hours/day to reduce the chances of developing depressive symptoms.

Male genitalia morphology and dichotomous key of the Neotropical genera of Abracrini (Orthoptera: Acrididae: Ommatolampidinae) grasshoppers.

The comparative analysis of the internal and external male genitalia of the Abracrini grasshoppers was conducted, including representatives of the type species of the genera, specimens compared with the same or more representative specimens of the genus, and bibliography data. We include schematic illustrations that point to the morphological differences between the genitalia, based on subgenital plate, furculae, epiproct, cerci, ectophallic sheath, aedeagal valves, expansion of gonopore and epiphallus. A dichotomous key to the 21 Abracrini genera was presented.

A RETROSPECTIVE STUDY OF HEPATIC HEMOSIDEROSIS AND IRON STORAGE DISEASE IN SEVERAL CAPTIVE AND FREE-RANGING AVIAN SPECIES.

Some wild species of mammals and birds are prone to excessive iron accumulation, especially when maintained in human care. Hemosiderosis is the process of intracellular accumulation of iron without evidence of toxicity, whereas hemochromatosis is characterized by severe iron accumulation with accompanying organ damage. Iron storage disease (ISD) occurs when organ damage is severe and causing clinical signs. This retrospective study investigated the occurrence of hemosiderosis and ISD across a variety of avian taxa, including captive and free-ranging birds. Archived paraffin-embedded hepatic samples from 103 birds from Belo Horizonte Zoo that died naturally in the period of 2008 to 2018 were re-evaluated with histologic and morphometric techniques, focusing on the identification and scoring of iron deposits in hepatocytes and the quantification of total affected hepatic area. The birds represented 13 orders, 22 families, and 52 genera, and 66 (64.0%) had some degree of iron accumulation in their liver. Importantly, no statistical difference was observed in the occurrence of iron accumulation between families, orders, or origin (free-ranging or captive). Direct and positive correlation was observed between the total area affected by the iron deposits and the histologic score. In this study, there were two cases with severe iron accumulation and clinical signs compatible with ISD: a barefaced curassow (Crax fasciolata) and a channel-billed toucan (Ramphastos vitellinus). This study indicates that iron accumulation may occur in a wide range of avian species, with frequencies and intensities that are similar between free-ranging birds and those in human care. It describes for the first time the occurrence of ISD in a Galliform species.

A universidade na pandemia de COVID-19: relato de experiência do laboratório de práticas emancipatórias e territoriais (lapet) / La universidad en la pandemia de COVID-19: relato de experiencia del laboratorio de prácticas emancipatorias y territoriales (lapet) / The university in COVID-19 pandemic: report of experience at the lab of emancipatory and territorial practices (lapet)

Diante da pandemia de COVID-19 no Brasil, as práticas sociais sofreram drásticas alterações, impactando instituições, coletivos e indivíduos. Dentre esses atores sociais, a terapia ocupacional e a universidade pública perceberam a necessidade de se reinventar para dar respostas adequadas às crises instauradas nesse complexo contexto, dado que, para além da questão sanitária, o Brasil enfrenta problemas de ordem econômica, ecológica, ética e política. Este relato tem o objetivo de apresentar as ações desenvolvidas pelo Laboratório de Práticas Emancipatórias e Territoriais, LAPET, durante a pandemia, ancorado no curso de Terapia Ocupacional de uma universidade federal do sul do Brasil. Diante das novas condições impostas pelo distanciamento social, fez-se necessário inovar as práticas de ensino, pesquisa e extensão. Diversas ações foram realizadas de forma unificada e coesa, com finalidade de defender as políticas sociais em direção à emancipação, sobretudo de trabalhadores da Assistência Social e da Saúde. Partiu-se de metodologias participativas, da dialogicidade e da priorização da horizontalização das relações. Processos educativos críticos que rompem com a forma escolar foram deflagrados junto a estudantes de graduação por meio de grupo de estudos e outros instrumentos e, também, junto a trabalhadores essenciais a partir de entrevistas individuais, oficinas emancipatórias e outros recursos tecnológicos. Considera-se que as práticas realizadas trazem um tensionamento do currículo regular do curso de Terapia Ocupacional, além de servirem como instrumento de apreensão das contradições da realidade, facilitando a busca por justiça e transformação social.

Frente a la pandemia de COVID-19 en Brasil, las prácticas sociales han sufrido cambios drásticos que impactan a instituciones, colectivos e individuos. Entre estos actores sociales, la terapia ocupacional y la universidad pública se dieron cuenta de la necesidad de reinventarse para dar respuestas adecuadas a las crisis establecidas en este contexto complejo, dado que, además del tema de salud, Brasil enfrenta problemas económicos, ecológicos, éticos y políticos. Este relato de experiencia tiene como objetivo presentar las acciones desarrolladas por el Laboratorio de Prácticas Emancipatorias y Territoriales, LAPET, durante la pandemia, anclado en el curso de terapia ocupacional de una universidad federal en el sur de Brasil. Ante las nuevas condiciones impuestas por la distancia social, fue necesario innovar las prácticas de docencia, investigación y extensión. Varias acciones se llevaron a cabo de manera unificada y cohesionada, con el propósito de defender las políticas sociales hacia la emancipación, especialmente de los trabajadores asistenciales y de salud. Se inició con metodologías participativas, dialogicidad y priorización de relaciones horizontales. Los procesos educativos críticos que rompen con la forma escolar fueron desencadenados por estudiantes de pregrado a través de grupos de estudio y otros instrumentos, y también por trabajadores esenciales por medio de entrevistas individuales, talleres emancipatorios y otros recursos tecnológicos. Se considera que las prácticas realizadas aportan tensión al currículo regular del curso de Terapia Ocupacional, además de servir como un instrumento para aprehender las contradicciones de la realidad, facilitando la búsqueda de justicia y transformación social.

During COVID-19 pandemic, social practices were drastically changed in Brazil. It impacted institutions, groups and individuals. Among them, occupational therapy and public university needed to recreate their practices, in order to properly respond to the crisis in this complex context, beyond the sanitary issue, Brazil faces economic, ecological, ethical and political problems. This report aims to inform the actions developed by the Lab of Emancipatory and Territorial Practices, (LAPET, by its initials in portuguese), anchored in the occupational therapy graduation course, at a federal university of the south of Brazil. Due to the new conditions imposed by social isolation, learning practices, research and extension were recreated. Coherent and unified actions were performed to advocate for social policies toward emancipation, specially concerning social assistance and health care workers. Participative methodologies were used, prioritizing dialog and horizontal relations. Critical educational that ruptures with school system were proceeded with under graduated students in group studies, and other instruments. Essential workers also participated of the educational processes through interviews, emancipatory workshops, and other technological resources. The driven practices bring tension to the regular occupational therapy graduation curriculum and become instruments of reality apprehension, benefiting social justice and transformation.

Adaptação cultural do questionário de avaliação de resultados em blefaroplastia: Blepharoplasty Outcomes Evaluation / Cultural adaptation of the blepharoplasty outcomes evaluation questionnarie: Blepharoplasty Outcomes Evaluation

Introdução: O emprego de questionários de qualidade de vida tem se mostrado muito útil no sentido de dar maior objetividade à avaliação de resultados de tratamentos. A internacionalização desses instrumentos, por sua vez, permite a comparação interpopulacional, mas requer uma metodologia específica, a fim de não causar distorções devido a falhas na tradução ou a diferenças culturais. O questionário Blepharoplasty Outcomes Evaluation, de língua inglesa, é uma ferramenta de simples aplicação, com perguntas objetivas com boa aplicação para esse fim. O questionário já foi testado em relação à sua confiabilidade, validade e capacidade de resposta. Métodos: Realizada tradução e adaptação cultural para a língua portuguesa, segundo a metodologia proposta por Beaton et al., na qual existem 5 estágios. Estágio 1 - tradução por meio de dois tradutores nativos de língua portuguesa. Estágio 2 - confecção de versão de síntese. Estágio 3 - tradução reversa por dois tradutores nativos de língua inglesa. Estágio 4 - revisão por um comitê avaliador. Estágio 5 - aplicação a uma população de 20 pessoas. Resultados: A partir do comitê avaliador, não houve problemas de compreensão para a população final. Conclusão: O questionário foi traduzido e adaptado com sucesso.

Introduction: The use of quality of life questionnaires has proved to be very useful in giving greater objectivity to evaluating treatment results. The internationalization of these instruments, in turn, allows for interpopulation comparison but requires a specific methodology in order not to cause distortions due to failures in translation or cultural differences. The Blepharoplasty Outcomes Evaluation questionnaire, in English, is a simple application tool with objective questions with a good application for this purpose. The questionnaire has already been tested for reliability, validity and responsiveness. Methods: According to the methodology proposed by Beaton et al., translation and cultural adaptation into Portuguese was performed with 5 stages. Stage 1 - translation by two native Portuguese-speaking translators. Stage 2 - preparation of the synthesis version. Stage 3 - reverse translation by two native English-speaking translators. Stage 4 - review by an evaluation committee. Stage 5 - application to a population of 20 people. Results: There were no comprehension problems for the final population from the evaluation committee. Conclusion: The questionnaire was successfully translated and adapted.

Single nCounter assay for prediction of MYCN amplification and molecular classification of medulloblastomas: a multicentric study.

PURPOSE: Medulloblastoma is the most frequent pediatric malignant brain tumor, and is divided into four main subgroups: WNT, SHH, group 3, and group 4. MYCN amplification is an important medulloblastoma prognostic biomarker. We aimed to molecular classify and predict MYCN amplification in a single assay. METHODS: It was included 209 medulloblastomas from 205 patients (Brazil, Argentina, and Portugal), divided into training (n = 50) and validation (n = 159) sets. A nCounter assay was carried out using a custom panel for molecular classification, with additional genes, including MYCN. nSolver 4.0 software and the R environment were used for profiling and MYCN mRNA analysis. MYCN amplification by FISH was performed in 64 cases. RESULTS: The 205 medulloblastomas were classified in SHH (44.9%), WNT (15.6%), group 3 (18.1%) and group 4 (21.4%). In the training set, MYCN amplification was detected in three SHH medulloblastomas by FISH, which showed significantly higher MYCN mRNA counts than non-FISH amplified cases, and a cutoff for MYCN amplification was established ([Formula: see text] + 4σ = 11,124.3). Applying this threshold value in the validation set, we identified MYCN mRNA counts above the cutoff in three cases, which were FISH validated. CONCLUSION: We successfully stratified medulloblastoma molecular subgroups and predicted MYCN amplification using a single nCounter assay without the requirement of additional biological tissue, costs, or bench time.

Willingness to pay for a hypothetical malaria vaccine in Brazil: a cross-sectional study and the implications.

Aim: Malaria is an infection caused by protozoa of genus Plasmodium, considered the one associated with increasingly large outbreaks. Methods: A cross-sectional study was conducted with residents in the northern region of Brazil on the willingness to pay (WTP) for a hypothetical vaccine against malaria (effective protection of 80%). Results: Of 616 people interviewed, most interviewees were female (61%) and were employed (97%). The median individual maximum WTP for a hypothetical malaria vaccine was US$11.90 (BRL 50). Conclusion: The northern region of Brazil is one of the largest markets for a malaria vaccine due to its epidemiological relevance. Consequently, economic studies will be important to assist in the assessment of the potential price and value of new vaccines.

Using the Robson classification to assess caesarean section rates in Brazil: an observational study of more than 24 million births from 2011 to 2017.

BACKGROUND: Applying the Robson classification to all births in Brazil, the objectives of our study were to estimate the rates of caesarean section delivery, assess the extent to which caesarean sections were clinically indicated, and identify variation across socioeconomic groups. METHODS: We conducted a population-based study using routine records of the Live Births Information System in Brazil from January 1, 2011, to December 31, 2017. We calculated the relative size of each Robson group; the caesarean section rate; and the contribution to the overall caesarean section rate. We categorised Brazilian municipalities using the Human Development Index to explore caesarean section rates further. We estimated the time trend in caesarean section rates. RESULTS: The rate of caesarean sections was higher in older and more educated women. Prelabour caesarean sections accounted for more than 54 % of all caesarean deliveries. Women with a previous caesarean section (Group 5) made up the largest group (21.7 %). Groups 6-9, for whom caesarean sections would be indicated in most cases, all had caesarean section rates above 82 %, as did Group 5. The caesarean section rates were higher in municipalities with a higher HDI. The general Brazilian caesarean section rate remained stable during the study period. CONCLUSIONS: Brazil is a country with one of the world's highest caesarean section rates. This nationwide population-based study provides the evidence needed to inform efforts to improve the provision of clinically indicated caesarean sections. Our results showed that caesarean section rates were lower among lower socioeconomic groups even when clinically indicated, suggesting sub-optimal access to surgical care.

Genetic divergence for adaptability and stability in sugarcane: Proposal for a more accurate evaluation.

The best agro-industrial performance presented by a crop genotype in one environment may not be reproduced in another owing to complex edaphoclimatic variations. Therefore, breeding programs are constantly attempting to obtain, through artificial hybridization, novel genotypes with high adaptability and stability potential. The objective of this study was to analyze genetic divergence in sugarcane based on the genotypic values of adaptability and stability. A total of 11 sugarcane genotypes were analyzed for eight agro-industrial traits. The genotypic values of the traits were determined using mixed model methodology, and the genetic divergence based on phenotypic and genotypic values was measured using the Mahalanobis distance. The distance matrices were correlated using the Mantel test, and the genotypes were grouped using the Tocher method. Genetic divergence is more accurate when based on genotypic values free of genotype-environment interactions and will differ from genetic divergence based on phenotypic data, changing the genotype allocations in the groups. The above methodology can be applied to assess genetic divergence to obtain novel sugarcane genotypes with higher productivity that are adapted to intensive agricultural systems using diverse technologies. This methodology can also be tested in other crops to increase accuracy in selecting the parents to be crossed.

ACUTE NECROTIZING AND EOSINOPHILIC MYOCARDITIS IN A CHIMPANZEE (PAN TROGLODYTES).

Cardiac disease is of importance in captive chimpanzee (Pan troglodytes) health. Here we report an eosinophilic and necrotizing myocarditis in a 17-y-old chimpanzee with no previous history of cardiac disease that progressed to death within 48 h. Toxic and infectious causes were ruled out. The chimpanzee had eosinophilia at different occasions in previous years. The animal had a severe, diffuse, and acute monophasic necrotizing myocarditis, with a moderate lymphoplasmacytic infiltrate that was rich in eosinophils. Ante- and postmortem investigations are compatible with an unusual eosinophilic myocarditis with clinical evolution and morphology comparable with human eosinophilic myocarditis secondary to hypereosinophilic syndrome.

Consumer willingness to pay for a hypothetical Chagas disease vaccine in Brazil: a cross-sectional study and the implications.

Aim: Chagas disease is a serious public health problem, endemic in 21 countries in Latin America. A future vaccine can contribute to decreasing the number of cases and its complications. Methods: A cross-sectional study was conducted with residents of the northern region of Brazil, on the willingness to pay for a hypothetical vaccine against Chagas disease (effective protection of 80%). Results: We interviewed 619 individuals and seven were excluded from the analysis and the value of willingness to pay was US$23.77 (100.00 BRL). Conclusion: The Northern region of Brazil is one of the largest markets for this vaccine, due to its epidemiological relevance, so economic studies with this vaccine will be important to assist in the assessment of technologies.

A poorer nutritional status impacts quality of life in a sample population of elderly cancer patients.

RATIONALE: Quality of Life (QoL) is impaired in cancer, and the elderly are particularly vulnerable to malnutrition. A diagnosis of cancer in elderly patients further exacerbates risks of negative health outcomes. Here we investigated associations between QoL and nutritional status in a sample population of mostly socially deprived elderly cancer patients. METHOD: 432 cancer patients were recruited for this cross-sectional study at point of admission to a tertiary referral hospital for cancer treatment. Patient-generated subjective global assessment (PG-SGA) assessed nutritional status. Functional assessment of cancer therapy- general (FACT-G) quantified QoL. Relationship between PG-SGA and QoL was assessed by Spearman correlation. PG-SGA outcomes were compared against FACT-G scores employing Mann-Whitney test. Bivariate Linear Regression Model was employed to investigate influences of sociodemographic, clinical and nutritional status upon QoL. RESULTS: 37.5% of participants were malnourished or at risk. 39% were illiterate and 54.6% had family income lower than minimum wage. Malnourished patients showed lower FACT-G scores (76.8 vs. 84.7; p = 0.000). Poor nutritional diagnosis was inversely correlated with all QoL domains. Bivariate regression analysis showed that lower PG-SGA scores (ßo = - 1.00; p = 0.000) contributed to FACT-G score deterioration, the male gender showed better QoL scores, and other clinical and sociodemographic variables did not show relationship. CONCLUSION: Poorer nutritional status was significantly associated with worsened physical, social, emotional and functional well-being QoL domains in elderly cancer patients. Poorer nutritional status is an independent risk factor for worsened QoL. Future policies aimed at particularly vulnerable populations may improve QoL and health outcomes.

Trends of karyotype evolution in the Neotropical long-legged crickets Phalangopsidae (Orthoptera, Grylloidea).

Phalangopsids are a diverse group of crickets found in all tropical and subtropical regions, and includes 1044 valid species. Up to now, only 22 species were studied cytologically, with the chromosome number ranging from 2n = 11 to 2n = 21. In this paper we studied the chromosomes of 12 phalangopsid species from different Brazilian biomes (eight of them reported for the first time), and we traced some trends on chromosomal derivation in this group, based on chromosome morphology and fundamental number. We found that in the phalangopsid species the karyotype concentrates a large amount of metacentric chromosomes, the result of successive centric fusions over evolutionary time. Moreover, pericentric inversions and translocations have been also important in the chromosomal derivation of these crickets.

Experiencias de puérperas sobre violencia obstétrica en la perspectiva fenomenológica / Experiences of puerperas on obstetric violence in the phenomenological perspective

Introducción: La violencia obstétrica caracteriza un tipo específico de violencia de género, perpetrada contra mujeres en trabajo de parto, parto y posparto en instituciones de salud. Objetivo: Entender la experiencia de las mujeres posparto sobre violencia obstétrica en un hospital público de maternidad. Métodos: Investigación fenomenológica realizada con 17 parturientas en 2017, a través de entrevistas guiadas por un cuestionario semiestructurado. Los discursos fueron analizados y organizados a partir de la Fenomenología de la percepción de Merleau-Ponty. Resultados: Se identificaron tres categorías: El deseo de parto normal y la barrera de resolución profesional; La deshumanización de las actitudes profesionales y la conciencia del mejor logro; El compañero presente y el acompañamiento resistente. Se identificaron ambigüedades en las percepciones experimentadas por las mujeres, con sentimientos de inseguridad a satisfacción con el servicio recibido de los profesionales de la salud. Conclusiones: Hay significados perceptivos de violencia obstétrica en los informes de parturientas, y existe la necesidad de cambios en las prácticas profesionales para calificar la atención obstétrica de una manera humana(AU)

Introduction: Obstetric violence is a specific type of gender violence, perpetrated against women in labor, delivery and postpartum in health institutions. Objective: To understand the experience of postpartum women concerning obstetric violence in a public maternity hospital. Methods: Phenomenological research carried out with seventeen parturients in 2017, through interviews guided by a semistructured questionnaire. Their discourses were organized and analyzed based on Merleau-Ponty's phenomenology of perception. Results: Three categories were identified: desire for vaginal delivery versus professional decision-making, dehumanization of professional attitudes and awareness of best achievements, partner presence and enduring companion. Ambiguities were identified in the perceptions experienced by women, with feelings from insecurity to satisfaction concerning the service received from health professionals. Conclusions: There are perceptual meanings regarding obstetric violence in the reports of women in labor. There is a need for changes in professional practices to qualify obstetric care in a humane way(AU)

Neopedies taimensis n. sp. of Brazilian Dichroplini (Orthoptera: Acrididae, Melanoplinae) from Rio Grande do Sul, Brazil.

Neopedies taimensis n. sp. is described as a new species of grasshopper from the genus Neopedies Hebard, 1931 collected in Taim Ecological Station, State of Rio Grande do Sul, Brazil. This description is supported with photographs that detail the male and female external and internal morphological characteristics. The new species differs from the other species known by furculae developed and sclerotized, epiproct with two protuberances, cerci quadrangular indented and sclerotized, ancorae developed and lophi with posterior projections divergent. In addition, we present habitat notes, geographic distribution, key to the species of genus and chromosome complement. Chromosome analyses were performed using standard staining procedures, showing diploid number of 2n = 23, X0/24, XX and the karyotype made up of exclusively acrocentric chromosomes.