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Nat Commun ; 11(1): 5345, 2020 10 22.
Artículo en Inglés | MEDLINE | ID: mdl-33093494

RESUMEN

Due to its longevity and enormous information density, DNA is an attractive medium for archival storage. The current hamstring of DNA data storage systems-both in cost and speed-is synthesis. The key idea for breaking this bottleneck pursued in this work is to move beyond the low-error and expensive synthesis employed almost exclusively in today's systems, towards cheaper, potentially faster, but high-error synthesis technologies. Here, we demonstrate a DNA storage system that relies on massively parallel light-directed synthesis, which is considerably cheaper than conventional solid-phase synthesis. However, this technology has a high sequence error rate when optimized for speed. We demonstrate that even in this high-error regime, reliable storage of information is possible, by developing a pipeline of algorithms for encoding and reconstruction of the information. In our experiments, we store a file containing sheet music of Mozart, and show perfect data recovery from low synthesis fidelity DNA.


Asunto(s)
Técnicas de Química Sintética/métodos , ADN/síntesis química , Almacenamiento y Recuperación de la Información/métodos , Algoritmos , Secuencia de Bases , ADN/química , ADN/genética , Biblioteca de Genes , Luz , Método de Montecarlo , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Procesos Fotoquímicos , Análisis de Secuencia de ADN
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