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Plant Biotechnol J ; 4(4): 393-407, 2006 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-17177805

RESUMEN

Endosperm accounts for a large proportion of human nutrition and is also a major determinant of seed viability and size, not only in cereals, but also in species with ephemeral endosperms, such as soybean and oilseed rape. The extent of endosperm proliferation early in seed development is a crucial component in setting seed size; therefore, a biotechnological approach for the modification of this trait requires promoters active in early endosperm. To find such promoters, we constructed an array based on cDNAs extracted from developing Arabidopsis seeds enriched for proliferating endosperm. Hybridization with RNA extracted from vegetative and reproductive tissues, including endosperm, and subsequent data filtering yielded sets of endosperm-expressed and endosperm-preferred genes, including many hundreds not previously identified in array experiments designed to detect genes expressed in Arabidopsis seeds. Of eight promoters selected for validation, seven were active in early endosperm, three with no detected activity elsewhere in the plant. Therefore, this strategy has yielded proliferative phase endosperm promoters which should be useful in altering seed size.


Asunto(s)
Arabidopsis/genética , Regiones Promotoras Genéticas , Semillas/genética , Arabidopsis/citología , Arabidopsis/crecimiento & desarrollo , Proliferación Celular , Cruzamientos Genéticos , Etiquetas de Secuencia Expresada , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Biblioteca de Genes , Genes de Plantas/fisiología , Genes Reporteros , Glucuronidasa/análisis , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas Recombinantes de Fusión/análisis , Semillas/citología , Semillas/crecimiento & desarrollo
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