Generalised exponential-Gaussian distribution: a method for neural reaction time analysis.

Reaction times (RTs) are an essential metric used for understanding the link between brain and behaviour. As research is reaffirming the tight coupling between neuronal and behavioural RTs, thorough statistical modelling of RT data is thus essential to enrich current theories and motivate novel findings. A statistical distribution is proposed herein that is able to model the complete RT's distribution, including location, scale and shape: the generalised-exponential-Gaussian (GEG) distribution. The GEG distribution enables shifting the attention from traditional means and standard deviations to the entire RT distribution. The mathematical properties of the GEG distribution are presented and investigated via simulations. Additionally, the GEG distribution is featured via four real-life data sets. Finally, we discuss how the proposed distribution can be used for regression analyses via generalised additive models for location, scale and shape (GAMLSS).

A multi-analyses approach of inductive/deductive asymmetry in the affective priming paradigm.

Rapidly evaluating our environment's beneficial and detrimental features is critical for our successful functioning. A classic paradigm used to investigate such fast and automatic evaluations is the affective priming (AP) paradigm, where participants classify valenced target stimuli (e.g., words) as good or bad while ignoring the valenced primes (e.g., words). We investigate the differential impact that verbs and adjectives used as primes and targets have on the AP paradigm. Based on earlier work on the Linguistic Category Model, we expect AP effect to be modulated by non-evaluative properties of the word stimuli, such as the linguistic category (e.g., if the prime is an adjective and the target is a verb versus the reverse). A reduction in the magnitude of the priming effect was predicted for adjective-verb prime-target pairs compared to verb-adjective prime-target pairs. Moreover, we implemented a modified crowdsourcing of statistical analyses implementing independently three different statistical approaches. Deriving our conclusions on the converging/diverging evidence provided by the different approaches, we show a clear deductive/inductive asymmetry in AP paradigm (exp. 1), that this asymmetry does not require a focus on the evaluative dimension to emerge (exp. 2) and that the semantic-based asymmetry weakly extends to valence (exp. 3).

Reference values for the cross-sectional area of the lumbar multifidus muscle in children.

BACKGROUND: The multifidus muscle plays a major role in the growth and postural control of children. Therefore, the reference values of the cross-sectional area (CSA) of the lumbar multifidus muscle represent an important tool for assessing muscle development and the early monitoring of musculoskeletal disorders. OBJECTIVE: The aim of this study was to provide percentile scores for the CSA of the lumbar multifidus muscle in eutrophic children aged 5 to 10 years. METHODS: This was a cross-sectional, observational study, involving 736 children. An anthropometric assessment was conducted and ultrasound (US) imaging was used to assess the CSA of the lumbar (vertebral level L5) multifidus muscle. The CSA was expressed as percentile scores. RESULTS: The CSA in the boys ranged from 1.8 cm2 to 5.3 cm2 and in girls from 1.9 cm2 to 5.9 cm. The CSA in the 50th percentile scores of both sexes was 3.4 cm2. There was an increase in the CSA between 5 and 7 years old in both sexes. The CSA presented a greater variance in girls than in boys. After 8 years of age, the multifidus CSA increased in girls and decreased in boys. CONCLUSION: The present study has provided percentile scores for the CSA of the lumbar multifidus muscle for eutrophic children aged 5 to 10 years. An increase was observed of the CSA of the multifidus muscle between the ages of 5 and 7 years and there were no differences in the CSA percentiles in relation to sex. An increase in the CSA after 8 years of age was only observed in the girls.

Disclosing contrasting scenarios for future land cover in Brazil: Results from a high-resolution spatiotemporal model.

Gaining information on the dynamics of land cover changes is a valuable step towards improving practical conservation actions. In recent years, the Brazilian presidential elections in 2018 and the recovery from one of the nation's worst economic recessions defined a political scenario that has been causing shifts in the patterns of land cover change. A variety of national plans for the near-future exist and include the construction of new roads connecting remote Amazonian areas and large dams that could flood up to 10 million hectares. These development plans threaten environmental conservation, but the potential effects on the local or regional land cover are mostly unknown. In this work, we construct a model to evaluate the possible consequences of policy actions on land cover dynamics in the near-future at a high-resolution scale. The regression model extracts the historical relationships between land cover and spatial drivers of change, and its extrapolation for the future enables the simulation of scenarios for the national plans currently discussed in Brazil. We also simulate three scenarios based on the Representative Concentration Pathways of the Intergovernmental Panel on Climate Change, which makes contrasting management assumptions. The resulting maps indicate that considerable changes in land cover composition and configuration may occur even in a short period. The historical Brazilian economic forces make the decrease in natural vegetation probabilities challenging to stop even in an environmentally oriented scenario, where plans for the construction of new infrastructure are abruptly interrupted. Our results also indicate that environmental degradation cannot be prevented without coordinated efforts between public agencies with a broad diversity of development viewpoints.

Efficacy of voriconazole in vitro and in invertebrate model of cryptococcosis.

Cryptococcosis is a common opportunistic infection in patients with advanced HIV infection and may also affect immunocompetent patients. The available antifungal agents are few and other options are needed for the cryptococcosis treatment. In this work, we first analyzed the virulence of twelve C. neoformans and C. gattii strains assessing capsule thickness, biofilms formation, and survival and morbidity in the invertebrate model of Galleria mellonella and then we evaluated the antifungal activity of voriconazole (VRC) in vitro and in vivo also using G. mellonella. Our results showed that all Cryptococcus spp. isolates were able to produce capsule and biofilms, and were virulent using G. mellonella model. The VRC has inhibitory activity on planktonic cells with MIC values ranging from 0.03 to 0.25 µg/mL on Cryptococcus spp.; and these isolates were more tolerant to fluconazole (ranging from 0.25 to 16 µg/mL), the triazol agent often recommended alone or in combination with amphotericin B in the cryptococcosis therapy. In contrast, mature biofilms were less susceptible to the VRC treatment. The VRC (10 or 20 mg/kg) treatment of infected G. mellonella larvae significantly increased the larval survival when compared to the untreated group for the both Cryptococcus species and significantly decreased the fungal burden and dissemination in the larval tissue. Our findings corroborate with the literature data, supporting the potential use of VRC as an alternative for cryptococcosis treatment. Here, we emphasize the use of G. mellonella larval model as an alternative animal model for studies of antifungal efficacy on mycosis, including cryptococcosis.

Alginate nanoparticles as non-toxic delivery system for miltefosine in the treatment of candidiasis and cryptococcosis.

INTRODUCTION AND OBJECTIVE: Previous studies indicate that miltefosine (MFS) may be an alternative as an antifungal agent; however, it presents several adverse effects. Thus, the aim of this study was to produce miltefosine-loaded alginate nanoparticles (MFS.Alg) for toxicity reduction to be used as an alternative for the treatment of cryptococcosis and candidiasis. METHODS: Alginate nanoparticles were produced using the external emulsification/gelation method, and their physicochemical and morphological characteristics were analyzed. MFS encapsulation efficiency, release assay and toxicity on red blood cells and on Galleria mellonella larvae were assessed. The antifungal activity was evaluated using in vitro and in vivo larval models of G. mellonella infected with Candida albicans (SC5314 and IAL-40), Cryptococcus neoformans H99 and Cryptococcus gattii ATCC 56990. The treatment efficacy was evaluated by survival curve, colony forming unit (CFU) counting and histopathological analysis. RESULTS: MFS.Alg nanoparticles presented a mean size of 279.1±56.7 nm, a polydispersity index of 0.42±0.15 and a zeta potential of -39.7±5.2 mV. The encapsulation efficiency of MFS was 81.70±6.64%, and its release from the nanoparticles occurred in a sustained manner. MFS in alginate nanoparticles presented no hemolytic effect and no toxicity in G. mellonella larvae. Treatment with MFS.Alg extended the survival time of larvae infected with C. albicans and C. gattii. In addition, the fungal burden reduction was confirmed by CFU and histopathological data for all groups treated with 200 mg/Kg of MFS.Alg. CONCLUSION: These results support the use of alginate-based drug delivery systems as carriers for MFS for drug toxicity reduction and control of the fungal infection in the in vivo model of G. mellonella.

Nanocarriers Provide Sustained Antifungal Activity for Amphotericin B and Miltefosine in the Topical Treatment of Murine Vaginal Candidiasis.

Topical drug administration is frequently used for the treatment of vaginal candidiasis; however, most formulations using this route do not provide prolonged drug release. Our aim was to evaluate the antifungal efficacy of amphotericin B (AMB) and miltefosine (MFS) incorporated in nanocarriers for sustained drug release, in a murine model of vaginal candidiasis. AMB and MFS were incorporated in different topical formulations, namely: conventional vaginal cream (daily dose for 6 days; MFS-CR and AMB-CR groups), microemulsion that transforms into a liquid crystalline gel in situ (single dose, or in three doses, every 48 h; AMB-ME and MFS-ME groups) and alginate nanoparticles (single dose; MFS-AN group). Formulations were administered intravaginally in BALB/c female mice 24 h post-infection by Candida albicans yeasts. On the 7th day post-infection the animals were euthanized for mycological and histological analyses. Formulation persistence in the vaginal canal was assessed for 7 days by in vivo imaging, using nanocarriers labeled with Alexa-Fluor 647. AMB-ME(3×), MFS-ME(3×), and MFS-AN(1×) formulations were able to control fungal infection at comparable levels to those vaginal cream formulations. Notably, a single dose of MFS-AN was sufficient to reduce the fungal burden as effectively as MFS-ME(3×) and MFS-CR(6×). In vivo imaging showed that nanocarriers allowed prolonged antifungal activity by intravaginal administration reducing drug administration frequency. Therefore, AMB and MFS incorporated into a microemulsion and MFS encapsulated in alginate nanoparticles could be effective therapeutic alternatives for vaginal candidiasis, likely due to the sustained antifungal activity provided by these nanocarriers.