Hydrodynamic cavitation for scalable exfoliation of few-layered graphene nanosheets.

A scalable manufacturing method for the production of biocompatible fewlayered graphene nanosheets is developed using hydrodynamic cavitation. Scalable exfoliation is induced by employing hydrodynamic cavitation and a serum albumin protein. Unlike acoustic cavitation, the primary means of bubble collapse in hydrodynamic cavitation is caused laterally, thereby separating two adjacent flakes through a shear effect. In this process, bovine serum albumin, a known protein, was employed to act as an effective exfoliation agent and provide desired stability by preventing restacking of the graphene layers. This method was used to study the effect of time of graphene exfoliation in a novel hydrodynamic cavitation system. The fabricated products were characterized using Raman spectroscopy, Transmission electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The results showed that with increasing the time of exfoliation, the number of graphene layers decreased based on theI2D/IGratio but disorder increased based on theID/IGratio. At 3 h, theI2D/IGratio was at 0.39 and theID/IGratio was 0.25, while at 6 h theI2D/IGratio was 0.35 andID/IGratio was 0.29. The results of the theoretical and computational analysis this research outlines are needed to obtain an effective cavitation model that can be used to potentially improve graphene synthesis and quality. The captured images of bubble propagation in the solution imply that this fluidic phenomenon could assist the graphene exfoliation. To prove this, a simple cavitation model using a needle valve was designed. The needle valve cavitation setup was able to identify that cavitation assists in graphene exfoliation and this was proved using the graphene characterization data. Based on these findings, the simulation models were designed in ANSYS and COMSOL. Specifically, through the ANSYS simulation, we were able to calculate cavitation numbers for specific flow rates and fluid temperatures.

Protein-assisted scalable mechanochemical exfoliation of few-layer biocompatible graphene nanosheets.

A facile method to produce few-layer graphene (FLG) nanosheets is developed using protein-assisted mechanical exfoliation. The predominant shear forces that are generated in a planetary ball mill facilitate the exfoliation of graphene layers from graphite flakes. The process employs a commonly known protein, bovine serum albumin (BSA), which not only acts as an effective exfoliation agent but also provides stability by preventing restacking of the graphene layers. The latter is demonstrated by the excellent long-term dispersibility of exfoliated graphene in an aqueous BSA solution, which exemplifies a common biological medium. The development of such potentially scalable and toxin-free methods is critical for producing cost-effective biocompatible graphene, enabling numerous possible biomedical and biological applications. A methodical study was performed to identify the effect of time and varying concentrations of BSA towards graphene exfoliation. The fabricated product has been characterized using Raman spectroscopy, powder X-ray diffraction, transmission electron microscopy and scanning electron microscopy. The BSA-FLG dispersion was then placed in media containing Astrocyte cells to check for cytotoxicity. It was found that lower concentrations of BSA-FLG dispersion had only minute cytotoxic effects on the Astrocyte cells.