RESUMEN
Enteric viruses are widely spread in water environments, some being harmful for human communities. Regular epidemics highlight the usefulness of analysing such viruses in wastewaters as a tool for epidemiologists to monitor the extent of their dissemination among populations. In this context, CNovel™ Powdered Activated Carbon (PAC) was chosen for its high porosity and high adsorption capacity to investigate sorbent ability to be used as part of of virus detection probes. Self-supported PAC Foils (PAC-F), PAC coated Brushes (PAC-B) and PAC Sampler (PAC-S) were used to prospect PAC efficacy in virus adsorption and above all, the feasibility of virus retrieval from them, allowing to further analysis such as molecular analysis quantification. Aiming at the development of a field-operational tool, PAC saturation and reusability were also investigated, as well as PAC-polarisation effect on its adsorption capacity. Our results pointed out that sorbent-based probes exhibited a high adsorption efficacy of spiked Murine Norovirus (MNV-1) in bare 0.1 M NaCl solution (>90 % for PAC-B and >86 % for PAC-F at ≈107 genome unit virus concentration), with no saturation within our experimental framework. On the other hand, polarisation assays using PAC-F as electrode, did not demonstrate any adsorption improvement. Experiments on PAC probes reusability suggested that they should be used three times at the most for a maximum efficiency. Values of virus retrieval were low (up to 11 % with PAC-B and up to 14 % with PAC-F in 0.1 M NaCl virus suspensions), illustrating the need for the techniques to be improved. A preliminary field assay using PAC-S, demonstrated that our catch-and-retrieve protocol yielded to the detection of autochthonous human Norovirus Genogroup I (NoV GI) and Adenovirus (AdV), in wastewaters suggesting its promising application as virus detection tool in such high loaded and complex waters.