Cadaver preservative properties of a solution composed of honey, ethyl alcohol, liquid paraffin, distilled water and citric acid: Experiments on rabbit cadavers.

The objective of this study is to assess the efficacy of a solution including honey, ethyl alcohol, liquid paraffin, distilled water and citric acid (HEFS) as a preservative for rabbit cadavers, serving as a potential substitute for formaldehyde. The cadavers underwent preservation using three distinct solutions: 10% formalin, 35% alcohol and HEFS. The cadavers were subjected to a total of four sampling events, occurring at 4-month intervals, in order to collect specimens for microanatomical, histological, microbiological, mycological, colourimetric, texture and odour analysis. In terms of hardness, suitability for dissection and joint mobility metrics, the cadavers fixed with HEFS had superior qualities to those fixed with formalin. The fixation quality of HEFS for histological analyses was deemed acceptable, except kidney and intestinal tissues. In texture analysis, differences only in the elasticity parameter (p < 0.05) in the same sampling period. A total of 10 (13.9) bacteria isolates were identified among which, Metasolibacillus meyeri 3 (30%) was predominantly followed by Staphylococcus aureus 2 (20%), Bacillus siamensis, Bacillus subtilis, Pseudarthrobacter oxydans, Bacillus licheniformis, Bacillus subtilis subsp. subtilis with a proportion of 1 (10%), respectively, by both microbiological and molecular analysis. However, no anaerobic bacteria and fungi were isolated. A considerable percentage of the students had the perception that HEFS was appropriate for utilization in laboratory settings due to its absence of unpleasant odours and detrimental impact on ocular and respiratory functions. In conclusion, we consider that HEFS may serve as a viable substitute for formalin solution in the preservation of rabbit cadavers.

Orbital shape in goat and sheep: Symmetric analysis.

The aim of this study is to evaluate orbital symmetry in goat and sheep skulls. For this purpose, a total of 83 skulls, including 50 sheep and 33 goat skulls, were used in the study. Geometric morphometry method was applied. For symmetric analysis, one side of each orbit was photographed twice and mirror images were created. There were 36 landmards marked to determine the outer limit of the orbita. As a result of the study, asymmetric components (fluctuating asymmetry and directional asymmetry) for shape were statistically significant in both goats and sheep (p < 0.0001). The first three principal components explained 52.558% of the total shape variation in goats and 61.245% in sheep. This value for symmetric components was 59.095% and 67.742% for goats and sheep, respectively, and 66.791% and 71.154% for asymmetric components. As a result of discriminant function analysis, right and left orbital shapes showed grouping characteristics with similar success rates according to species. Although limited, the right orbit in goats (100%) and the left orbit in sheep (96.5%) were grouped more accurately.

Orbital shape in sheep and goats with sex and breed factors.

The purpose of this study was to identify the dimorphic structures of the orbita based on breed and sex factors through shape analysis across species. Additionally, the study aimed to ascertain the variability between the two species. A total of 86 (51 sheep-Akkaraman [Ak] and Morkaraman [Mk], 35 goats-Hair [Hr] and Honamli [Hm]) skulls were used in the study. It was decided to designate 36 landmarks to represent the orbit's edge. In the principal component analysis, 68 PCs were calculated. It was determined that PC1, PC2 and PC3 explained the total variance in morphology by 26.909%, 16.147% and 13.616%, respectively. According to the cross-validation results, the goats were grouped with 95% (Hm: 100%, Hr: 90%), while the sheep were grouped with 56% (Ak: 50%, Mk: 63%). Therefore, dimorphism among goat breeds on orbita shape was more pronounced compared to sheep. In the discriminant function analysis of the sex groups, Procrustes and Mahalanobis distance values in goats were 0.04425425 (p < 0.0001) and 5.3733 (p = 0.9550), in sheep 0.03513424 (p < 0.0001) and 7.1003 (p = 0.9414), respectively, was detected. According to the cross-validation results, in terms of sex, goats were correctly grouped at a rate of 74% (F: 73%; M: 75%) and sheep breeds were correctly grouped at a rate of 72% (F: 77%; M: 66%). Factors such as habitat selection, breeding behaviours and ecological environment may show anatomical differences within breeds as well as between breeds. Therefore, various measurement techniques are used to reveal these differences. As a result, it is thought that the study will contribute to many disciplines, especially taxonomy and zooarchaeology, by analysing the shape of the orbita of sheep and goat breeds, the domestication of which dates back to ancient times, and revealing the similarities and differences between species and according to sex.

Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) palm civet: Geometric morphometric analysis using palate, tooth and frontal landmarks.

This study aims to determine the differences in skull between Asian and golden palm civets by geometric morphometric method. The landmarks were marked on these photographs, and principal component, classical cluster and canonical variance analyses were performed. The method of geometric morphometry of the skull is a powerful tool used to differentiate the species. Fourteen and 20 homologous landmarks were marked using tpsutil and tpsdig2 programs on the frontal and palatinal directional photographs, respectively. As a result of the study, the first principal component (PC1) accounted for 33.586% on the frontal view, 41.602% on the palatinal view of the total shape differences, respectively. Distinctive differences on the frontal and palatinal view were observed in the caudal, rostrolateral and the last molars and the caudal palatine, respectively.

A pilot study: Can calcaneus radiographic image be used to determine sex and breed in cats?

This study examined whether radiographic images measurements of the calcaneus in cats are determinative of sex and breed. For this purpose, radiographic images of 70 cats (37 male and 33 females) of different ages (from one to 18 years) and different breeds (41 mix-breed, 18 Scottish Fold and 11 British Shorthair cats) without orthopaedic problems were used. Right tarsal joint radiographs of these orthopaedically healthy cats were taken. Four linear measurements and two angle values of the calcaneus were obtained from the radiographic images. The MANOVA result showed that the most determining factor between the three groups was the greatest width (p value = 0.001). Calcaneal body length, calcaneal greatest length and calcaneal shortest depth were higher in mix-breed cats. Calcaneal tuber length was higher in Scottish Fold cats. The only statistically significant difference between Scottish Fold and British Shorthair was in the calcaneal tuber length (p value = 0.04). In the comparison made between the sexes regardless of species, the linear measurements in males were higher than in females. It was determined that these parameters are statistically significant in terms of sex differentiation in cats. Dorsal and plantar calcaneal angles are not sex determinants in cats. The effect of age on other measurements was analysed by correlation test. However, the effect of age on the measurements was not statistically significant. Mix-breed cats were examined in four groups according to their colour (grey, black-white, yellow, tri-colour). No statistically significant difference was found between calcaneal measurements of cats with different skin colour genotypes. In this study, calcaneus measurements were both determinative between breeds and sexes in cats.

Geometric analysis of otoliths in Cyprinion kais and Cyprinion macrostomus.

Cyprinion macrostomus and Cyprinion kais are species that inhabit in the Tigris River. These fishes, formerly considered as the same species, have been listed as different species by taking differences in jaw and mouth structures into consideration. The mouth shape of C. kais is distinct and small. The mouth is convex and has broad lateral lobes. The mouth of C. macrostomus is wider, is convex and lacks lateral lobes. This study aimed to determine the shape-dependent variability in the breed and sex factors of otoliths of the fish species C. kais and C. macrostomus. For this purpose, 30 right otoliths from both species were used. Geometric morphometric analyses were performed by marking semilandmarks on the otoliths. When examining the results of the analysis, it was found that the first base component accounted for the total shape difference by 26.871% and the first three base components accounted for the total shape difference by 52.235%. In the regression analysis of shape on dimension, it was found that 2.1020% of shape could be estimated in terms of size according to the groups. In the wire-frame wrap graph showing group-based shape differences, it was found that C. kais was wider than C. macrostomus at the level of the dorsal margin and the rostrum region of C. macrostomus was larger and sharply pointed. Consequently, it is believed that the data of the study can be reference for many disciplines, especially for taxonomic classification of species.

Investigating metacarpi of Hamdani sheep via different measurement and modelling methods: A methodological study.

The aim of the study was to perform the morphometric analyses on metacarpi of Hamdani sheep via different measurement methods and to compare the methods. Thus, metacarpal bones of 15 females and 15 males were used in the study. Measurements were taken from the measurement points determined on metacarpus via four methods, and their statistical analyses were performed. When both male and female metacarpal bones were examined in the De parameter in the comparison with the methods, it was observed that the four measurement methods were different from one another (p < 0.01). When DEM and DEL measurement parameters were examined in male metacarpus, the highest value was measured in ImageJ and the smallest value was measured in 3D Slicer software; however, it was found that the statistical difference was not significant when compared numerically (p > 0.05). As a result, while it was observed that the measurements made by using digital calliper measurements, photometric analysis, and 3D software were similar in some of the parameters but there was no statistically significant difference; whereas, Statistical difference was detected in some parameters.

Intracranial arteries of New Zealand rabbits; identification, three-dimensional modelling, and morphometry by computed tomography angiography.

The aim of the study was to determine identification, three-dimensional modelling, and morphometry of intracranial arteries in New Zealand Rabbit by using computed tomography angiography. A total of 20 adult New Zealand Rabbits from both sexes were used in the study. General anaesthesia procedure was followed before computed tomography angiography imaging. Rabbits were placed in a prone position for imaging. Computed tomography angiography imaging was performed by injecting contrast agent into vena auricularis marginalis of rabbits. Morphometric measurements of the specified points were taken by MIMICS program using the computed tomography angiography images acquired. Three-dimensional intracranial artery model was prepared from the two-dimensional images on the same program. Measurements were statistically compared in terms of sex and side. It was observed in the study that arteria basilaris was generated by bilateral arteria vertebralis at the basal level of medulla oblongata. In the study, a statistically significant difference was determined only in diameter values of arteria cerebri caudalis dextra et sinistra in both female and male rabbits. On the other hand, no statistically significant difference was determined with respect to the side. Consequently, we think that the data of the present study will contribute to further studies on cerebrovascular pathology, clinicians or researchers.

Macroanatomic and morphometric analysis of the brown bear (Ursus arctos horribilis) mandible / Análisis macro anatómico y morfométrico de la mandíbula del oso pardo (Ursus arctos horribilis)

SUMMARY: Brown bear (Ursus arctos horribilis) is a wild animal from the bear (Ursidae) family. In this study, it was aimed to determine the morphometric values and anatomical structure of the brown bear mandible. After the superficial muscles of the mandible were dissected, the muscles were completely separated from the bones by boiling. 17 morphometric measurements were taken from the right and left mandible of each animal with the help of digital calipers. The mean and standard deviation values of the taken morphometric measurements were analyzed in the SPSS (20.0 version) package program. The mandible length was measured as 250.37 ± 9.75 mm on the right side and 246.83 ± 5.92 mm on the left side. The mandible height was determined as 105.76 ± 4.18 mm on the right and 108.62 ± 3.33 mm on the left. Consequently, the mandible was submitted to the results of the brown bear in the diversity of wildlife found in Turkey. We believe that the presented results will contribute to anatomical, surgical and archaeological studies.

RESUMEN: El oso pardo (Ursus arctos horribilis) es un animal salvaje de la familia de los osos (Ursidae). El objetivo de este estudio fue determinar los valores morfométricos y la estructura anatómica de la mandíbula del oso pardo, luego de la disección de los músculos superficiales de la mandíbula. Los músculos fueron separados por completo de los huesos mediante ebullición. Se tomaron 17 medidas morfométricas de la mandíbula derecha e izquierda de cada animal con la ayuda de calibradores digitales. Los valores de desviación estándar y media de las medidas morfométricas tomadas se anali- zaron en el programa SPSS (versión 20.0). El largo determiando de la mandíbula fue de 250,37 ± 9,75 mm en el lado derecho y 246,83 ± 5,92 mm en el lado izquierdo; la altura de la mandíbula era de 105,76 ± 4,18 mm en el lado derecho y 108,62 ± 3,33 mm en el izquierdo. Los resultados morfométricos obtenidos de la mandíbula del oso pardo en la diversidad de vida silvestre que se encuentra en Turquía contribuirán con el conocimiento anatómico y para los estudios quirúrgicos y arqueológicos.

The lumbosacral Plexus of the Barn Owl (tyto alba): Formation, branches and distribution.

The aim of the present study is to reveal the anatomical structure of lumbosacral plexus of barn owl. Six barn owls were included in the study. Nerves originating from plexus were dissected individually, and findings of innervation areas of nerves were determined. Lumbosacral plexus of barn owl was observed to be formed by ventral rami of ten synsacral spinal nerves. It was found that while the r. cutaneus femoris lateralis, the n. obturatorius, the n. coxalis cranialis, the r. cutaneus femoris medialis, the n. cutaneus femoris cranialis and the n. femoralis originated from the lumbar plexus, the n. isciofemoralis, the n. cutaneus femoris caudalis, the n. coxalis caudalis and the n. ischiadicus originated from the sacral plexus. Consequently, when the results of the study were compared with information of different avian species, it was observed that differences focused on the formation of the lumbar and sacral plexuses and innervation level of digits.

Macroanatomical features of brachial plexus and its branches in Günther's vole (Microtus guentheri).

In the present study the comprisal of brachial plexus and nerves springing from the brachial plexus in Günther's vole has been determined. Ten adult voles (five males and five females) were used in this study. Nerves comprising the brachial plexus and the nerves arising from brachial plexus in vole were dissected and the findings were saved. The brachial plexus of the vole was comprised of the ventral root of cervical (C) 5th, 6th, 7th and 8th and thoracic (T) 1st spinal nerves. Also, the brachial plexus was consisted of three trunks including cranial (C5th and C6th), medial (C7th) and caudal (C8th and T1st) trunks. Nervus (n.) subclavius, n. suprascapularis, nervi (nn.) subscapularis, n. axillaris, n. musculocutaneus, n. thoracodorsalis, nn. pectorales craniales, n. pectorales caudales, n. thoracicus lateralis, n. thoracicus longus, n. ulnaris, n. medianus, and n. radialis were determined to be arising from brachial plexus. In conclusion, macroanatomical features of brachial plexus and its branches in Günther's vole have been determined. It is thought that the findings will contribute to neuroanatomy of the rodents.