RESUMEN
A zinc acetate borate, [ZnAc]·[ZnBO3] (1), was synthesized under mild conditions; the B@Zn2O3 layer of 1 contains a 6-membered ring embedded with a rare BO3 unit. The layers are pillared by acetate ions to form a 3D framework. The pillared structure of 1 supplies enough space as a nanoreactor, and the related application of CO2-to-CO reduction has been confirmed.
RESUMEN
The first column of two-dimensional (2D)-layered metal-complex-templated nickel borate was obtained by using a solvothermal synthesis method. The 2D nickel borate [Ni(en)3]·[Ni0.5B6O8(OH)4]·Cl (1; en = ethylenediamine) contains [Ni(en)3]2+, Cl-, and the first reported inorganic nickel borate layer [Ni0.5B6O8(OH)4]-, which is formed by the interconnection of Ni and O atoms in the B-O cluster. By increasing the reaction temperature during the synthesis process of compound 1, the one-dimensional (1D) chain nickel borate [Ni(en)3]2·[B7O10(OH)3]·Cl2 (2) was obtained, wherein the [B7O10(OH)3] cluster was connected through an O atom to form a 1D chain structure. By adjusting the molar ratio of the raw materials, that is, adjusting the pH of the reaction, the other 1D chain nickel borate Ni(en)3·Hen·[B9O13(OH)4]·H2O (3) was obtained, in which [B5O8(OH)2] and [B4O7(OH)2] clusters can be connected via a common O atom to form an infinite [B9O13(OH)4] polyanionic chain. Meanwhile, we successfully synthesized the isoform of compound 3 with the metal cadmium.
RESUMEN
BACKGROUND: Enterovirus 71 (EV71) is a highly infectious agent that plays an etiological role in hand, foot, and mouth disease. It is associated with severe neurological complications and has caused significant mortalities in recent large-scale outbreaks. Currently, no effective vaccine or specific clinical therapy is available against EV71. METHODS: Unmodified 21 nucleotide small interfering RNAs (siRNAs) and classic 2'-modified (2'-O-methylation or 2'-fluoro modification) siRNAs were designed to target highly conserved 5' untranslated region (UTR) of the EV71 genome and employed as anti-EV71 agents. Real-time TaqMan RT-PCR, western blot analysis and plaque assays were carried out to evaluate specific viral inhibition by the siRNAs. RESULTS: Transfection of rhabdomyosarcoma (RD) cells with siRNAs targeting the EV71 genomic 5' UTR significantly delayed and alleviated the cytopathic effects of EV71 infection, increased cell viability in EV71-infected RD cells. The inhibitory effect on EV71 replication was sequence-specific and dosage-dependent, with significant corresponding decreases in viral RNA, VP1 protein and viral titer. Appropriate 2'-modified siRNAs exhibited similar RNA interference (RNAi) activity with dramatically increased serum stability in comparison with unmodified counterparts. CONCLUSION: Sequences were identified within the highly conserved 5' UTR that can be targeted to effectively inhibit EV71 replication through RNAi strategies. Appropriate 2'-modified siRNAs provide a promising approach to optimizing siRNAs to overcome barriers on RNAi-based antiviral therapies for broader administration.