Three-dimensional porous SERS powder for sensitive liquid and gas detections fabricated by engineering dense "hot spots" on silica aerogel.

A three-dimensional porous SERS powder material, Ag nanoparticles-engineered-silica aerogel, was developed. Utilizing an in situ chemical reduction strategy, Ag nanoparticles were densely assembled on porous aerogel structures, thus forming three-dimensional "hot spots" distribution with intrinsic large specific surface area and high porosity. These features can effectively enrich the analytes on the metal surface and provide huge near field enhancement. Highly sensitive and homogeneous SERS detections were achieved not only on the conventional liquid analytes but also on gas with the enhancement factor up to ∼108 and relative standard deviation as small as ∼13%. Robust calibration curves were obtained from the SERS data, which demonstrates the potential for the quantification analysis. Moreover, the powder shows extraordinary SERS stability than the conventional Ag nanostructures, which makes long term storage and convenient usage feasible. With all of these advantages, the porous SERS powder material can be extended to on-site SERS "nose" applications such as liquid and gas detections for chemical analysis, environmental monitoring, and anti-terrorism.

Self-assembled full nanowire P(VDF-TrFE) films with both anisotropic and high bidirectional piezoelectricity.

With the explosive growth of flexible electronics, the prototype piezoelectric polymer poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] has gained tremendous attention due to potential applications in flexible sensors, energy harvesters, and new smart devices. However, full realization of these applications is still challenging due to the lack of high quality films with strong piezoelectricity, which requires tailored molecular organization. Here we report unique 'full nanowire' P(VDF-TrFE) films with substantially enhanced bidirectional performance by a simple self-assembly via selective vapor annealing. Structural analysis showed that the solvent molecules significantly enhanced the copolymer chain mobility, giving highly ordered nanowires, whose quantity increased with time and finally formed a full flat-on lamellar nanowire array with backbones highly aligned along the film plane, leading to high lateral piezoelectricity as revealed by vector piezoresponse force microscopy and confirmed by electrical measurements. Surprisingly, the nanowire films also showed a much higher vertical piezoelectric coefficient (-35.2 pC N-1 directly measured by using a Berlincourt meter) than that of usually crystallized films owing to simultaneously enhanced molecular order and dipole switching ability. The scalability of the new method might boost industrial applications, and the findings may provide hints on new routes to nanostructured polymers with novel functionalities and deepen our understanding of the self-assembly of random copolymers.

Recent Advances in Protein Kinase Activity Analysis Based on Nanomaterials.

Protein phosphorylation regulated by protein kinases, as well as their dephosphorylation, is one of the most common post-translational modifications, and plays important roles in physiological activities, such as intracellular signal communications, gene transcription, cell proliferation and apoptosis. Over-expression of protein kinases is closely associated with various diseases. Consequently, accurate detection of protein kinases activities and their relevant inhibitors screening is critically important, not only to the biochemical research, but also to the clinical diagnosis and therapy. Nanomaterials, taking advantage of large surface areas, as well as excellent electrical, catalytic, magnetic and optical properties, have been utilized as target concentrators, recognition components, signal transducer or amplification elements in protein kinase related assays. This review summarizes the recent representative works to highlight the applications of nanomaterials in different biosensor technologies for protein kinases activities detection and their inhibitors screening. First, different nanomaterials developed for phosphoprotein/phosphopeptide enrichment and phosphate recognition are introduced. Next, representative works are selected that mainly focus on the utilization of nanomaterials as signal transducer or amplification elements in various protein kinases sensing platforms, such as electrochemical, colorimetric, fluorescent, and mass spectroscopy-based approaches. Finally, the major challenges and perspectives of nanomaterials being applied in protein kinases related assays are discussed.

Sensitive electrogenerated chemiluminescence biosensors for protein kinase activity analysis based on bimetallic catalysis signal amplification and recognition of Au and Pt loaded metal-organic frameworks nanocomposites.

In this work, a novel and sensitive electrogenerated chemiluminescence (ECL) biosensor for protein kinase A (PKA) activity analysis and relevant inhibitor screening was proposed based on bimetallic catalysis signal amplification and recognition of Au and Pt nanoparticles loaded metal-organic frameworks (Au&Pt@UiO-66) nanocomposite. After being phosphorylated by PKA in the presence of ATP, Au&Pt@UiO-66 probes were specifically chelated to the modified electrode by forming Zr-O-P bonds between the surface defects of UiO-66 and the phosphorylated kemptide. Due to the high synergistic catalysis of Au&Pt@UiO-66 nanocomposites to the luminol-H2O2 reaction, the ECL signal of luminol was greatly enhanced. Moreover, UiO-66 afford numerous Zr defect sites for high efficient phosphate group recognition, and can also prevent the nanoparticles from aggregating during catalytic reactions. Thus, the excellent performance of the ECL biosensor with high sensitivity and superior stability was obtained. Under the optimized conditions, the detection limit for PKA activity was 0.009 UmL-1 (S/N = 3). Meanwhile, the ECL biosensor was successfully applied in inhibitor screening and cell lysates PKA activity analysis, showing great promise in kinase related research.

Can odors of TCM be captured by electronic nose? The novel quality control method for musk by electronic nose coupled with chemometrics.

Musk is a precious and wide applied material in traditional Chinese medicine, also, an important material for the perfume industry all over the world. To establish a rapid, cost-effective and relatively objective assessment for the quality of musk, different musk samples, including authentic, fake and adulterate, were collected. A oxide sensor based electronic nose (E-nose) was employed to measure the musk samples, the E-nose generated data were analyzed by principal component analysis (PCA), the responses of 18 sensors of E-nose were evaluated by loading analysis. Results showed that a rapid evaluation of complex response of the samples could be obtained, in combination with PCA and the perception level of the E-nose was given better results in the recognition values of the musk aroma. The authentic, fake and adulterate musk could be distinguished by E-nose coupled with PCA, sensor 2, 3, 5, 12, 15 and 17 were found to be able to better discriminate between musk samples, confirming the potential application of an electronic instrument coupled with chemometrics for a rapid and on-line quality control for the traditional medicines.