Asunto(s)
Enfermedades de los Perros/diagnóstico , Hiperparatiroidismo/veterinaria , Paraproteinemias/veterinaria , Animales , Enfermedades de los Perros/cirugía , Perros , Femenino , Hiperparatiroidismo/diagnóstico , Paraproteinemias/diagnóstico , Neoplasias de las Paratiroides/cirugía , Neoplasias de las Paratiroides/veterinariaAsunto(s)
Camélidos del Nuevo Mundo/inmunología , Cadenas Pesadas de Inmunoglobulina/biosíntesis , Cadenas Pesadas de Inmunoglobulina/química , Animales , Anticuerpos Monoclonales , Afinidad de Anticuerpos , Formación de Anticuerpos , Especificidad de Anticuerpos , Reacciones Antígeno-Anticuerpo , Epítopos/química , Inmunización , Cadenas Pesadas de Inmunoglobulina/genética , Cadenas Pesadas de Inmunoglobulina/ultraestructura , Cadenas Ligeras de Inmunoglobulina/análisis , Región Variable de Inmunoglobulina/análisis , Modelos MolecularesRESUMEN
In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.
Asunto(s)
Complejo Antígeno-Anticuerpo/química , Complejo Antígeno-Anticuerpo/inmunología , Sitios de Unión de Anticuerpos , Muramidasa/química , Muramidasa/inmunología , Animales , Camelus , Pollos/inmunología , Cristalografía por Rayos X , Clara de Huevo , Femenino , Región Variable de Inmunoglobulina/química , Región Variable de Inmunoglobulina/inmunología , Modelos Moleculares , Fragmentos de Péptidos/química , Fragmentos de Péptidos/inmunología , Conformación ProteicaRESUMEN
Detailed knowledge on antibody-antigen recognition is scarce given the unlimited antibody specificities of which only few have been investigated at an atomic level. We report the crystal structures of an antibody fragment derived from a camel heavy chain antibody against carbonic anhydrase, free and in complex with antigen. Surprisingly, this single-domain antibody interacts with nanomolar affinity with the antigen through its third hypervariable loop (19 amino acids long), providing a flat interacting surface of 620 A(2). For the first time, a single-domain antibody is observed with its first hypervariable loop adopting a type-1 canonical structure. The second hypervariable loop, of unique size due to a somatic mutation, reveals a regular beta-turn. The third hypervariable loop covers the remaining hypervariable loops and the side of the domain that normally interacts with the variable domain of the light chain. Specific amino acid substitutions and reoriented side chains reshape this side of the domain and increase its hydrophilicity. Of interest is the substitution of the conserved Trp-103 by Arg because it opens new perspectives to 'humanize' a camel variable domain of heavy chain of heavy chain antibody (VHH) or to 'camelize' a human or a mouse variable domain of heavy chain of conventional antibody (VH).
Asunto(s)
Anticuerpos/inmunología , Anhidrasas Carbónicas/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos/genética , Afinidad de Anticuerpos , Especificidad de Anticuerpos , Camélidos del Nuevo Mundo , Humanos , Datos de Secuencia Molecular , Mutación , Alineación de SecuenciaRESUMEN
Arsenate reductase (ArsC) encoded by Staphylococcus aureus arsenic-resistance plasmid pI258 reduces intracellular As(V) (arsenate) to the more toxic As(III) (arsenite), which is subsequently extruded from the cell. ArsC couples to thioredoxin, thioredoxin reductase, and NADPH to be enzymatically active. A novel purification method leads to high production levels of highly pure enzyme. A reverse phase method was introduced to systematically analyze and control the oxidation status of the enzyme. The essential cysteinyl residues and redox couple in arsenate reductase were identified by a combination of site-specific mutagenesis and endoprotease-digest mass spectroscopy analysis. The secondary structures, as determined with CD, of wild-type ArsC and its Cys mutants showed a relatively high helical content, independent of the redox status. Mutation of Cys 10, 82, and 89 led to redox-inactive enzymes. ArsC was oxidized in a single catalytic cycle and subsequently digested with endoproteinases ArgC, AspN, and GluC. From the peptide-mass profiles, cysteines 82 and 89 were identified as the redox couple of ArsC necessary to reduce arsenate to arsenite.
Asunto(s)
Adenosina Trifosfatasas/química , Bombas Iónicas , Complejos Multienzimáticos , Staphylococcus aureus/enzimología , Adenosina Trifosfatasas/biosíntesis , Adenosina Trifosfatasas/genética , Adenosina Trifosfatasas/aislamiento & purificación , Secuencia de Aminoácidos , ATPasas Transportadoras de Arsenitos , Catálisis , Cromatografía Líquida de Alta Presión , Cromatografía por Intercambio Iónico , Dicroismo Circular , Cisteína/genética , Escherichia coli/genética , Espectrometría de Masas , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Oxidación-Reducción , Estructura Secundaria de Proteína , Reductasa de Tiorredoxina-Disulfuro/biosíntesis , Reductasa de Tiorredoxina-Disulfuro/genéticaRESUMEN
BACKGROUND: Camelid serum contains a large fraction of functional heavy-chain antibodies - homodimers of heavy chains without light chains. The variable domains of these heavy-chain antibodies (VHH) have a long complementarity determining region 3 (CDR3) loop that compensates for the absence of the antigen-binding loops of the variable light chains (VL). In the case of the VHH fragment cAb-Lys3, part of the 24 amino acid long CDR3 loop protrudes from the antigen-binding surface and inserts into the active-site cleft of its antigen, rendering cAb-Lys3 a competitive enzyme inhibitor. RESULTS: A dromedary VHH with specificity for bovine RNase A, cAb-RN05, has a short CDR3 loop of 12 amino acids and is not a competitive enzyme inhibitor. The structure of the cAb-RN05-RNase A complex has been solved at 2.8 A. The VHH scaffold architecture is close to that of a human VH (variable heavy chain). The structure of the antigen-binding hypervariable 1 loop (H1) of both cAb-RN05 and cAb-Lys3 differ from the known canonical structures; in addition these H1 loops resemble each other. The CDR3 provides an antigen-binding surface and shields the face of the domain that interacts with VL in conventional antibodies. CONCLUSIONS: VHHs adopt the common immunoglobulin fold of variable domains, but the antigen-binding loops deviate from the predicted canonical structure. We define a new canonical structure for the H1 loop of immunoglobulins, with cAb-RN05 and cAb-Lys3 as reference structures. This new loop structure might also occur in human or mouse VH domains. Surprisingly, only two loops are involved in antigen recognition; the CDR2 does not participate. Nevertheless, the antigen binding occurs with nanomolar affinities because of a preferential usage of mainchain atoms for antigen interaction.
Asunto(s)
Complejo Antígeno-Anticuerpo/química , Camelus/inmunología , Cadenas Pesadas de Inmunoglobulina/química , Conformación Proteica , Ribonucleasa Pancreática/química , Secuencia de Aminoácidos , Animales , Afinidad de Anticuerpos , Especificidad de Anticuerpos , Reacciones Antígeno-Anticuerpo , Sitios de Unión de Anticuerpos , Bovinos , Cristalografía por Rayos X , Humanos , Cadenas Pesadas de Inmunoglobulina/inmunología , Ratones , Modelos Moleculares , Datos de Secuencia Molecular , Páncreas/enzimología , Ribonucleasa Pancreática/inmunología , Programas Informáticos , Especificidad de la EspecieRESUMEN
Evidence is provided that dromedary heavy-chain antibodies, in vivo-matured in the absence of light chains, are a unique source of inhibitory antibodies. After immunization of a dromedary with bovine erythrocyte carbonic anhydrase and porcine pancreatic alpha-amylase, it was demonstrated that a considerable amount of heavy-chain antibodies, acting as true competitive inhibitors, circulate in the bloodstream. In contrast, the conventional antibodies apparently do not interact with the enzyme's active site. Next we illustrated that peripheral blood lymphocytes are suitable for one-step cloning of the variable domain fragments in a phage-display vector. By bio-panning, several antigen-specific single-domain fragments are readily isolated for both enzymes. In addition we show that among those isolated fragments active site binders are well represented. When produced as recombinant protein in Escherichia coli, these active site binders appear to be potent enzyme inhibitors when tested in chromogenic assays. The low complexity of the antigen-binding site of these single-domain antibodies composed of only three loops could be valuable for designing smaller synthetic inhibitors.
Asunto(s)
Inhibidores de Anhidrasa Carbónica , Inhibidores Enzimáticos/inmunología , Cadenas Pesadas de Inmunoglobulina/inmunología , Región Variable de Inmunoglobulina/inmunología , alfa-Amilasas/antagonistas & inhibidores , Secuencia de Aminoácidos , Animales , Afinidad de Anticuerpos , Especificidad de Anticuerpos , Camelus , Anhidrasas Carbónicas/inmunología , Anhidrasas Carbónicas/metabolismo , Bovinos , Inhibidores Enzimáticos/aislamiento & purificación , Humanos , Cadenas Pesadas de Inmunoglobulina/clasificación , Cadenas Pesadas de Inmunoglobulina/genética , Cadenas Pesadas de Inmunoglobulina/aislamiento & purificación , Región Variable de Inmunoglobulina/clasificación , Región Variable de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/aislamiento & purificación , Masculino , Ratones , Datos de Secuencia Molecular , Proteínas Recombinantes de Fusión , Alineación de Secuencia , Porcinos , alfa-Amilasas/inmunologíaRESUMEN
Functional heavy-chain gamma-immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins.
Asunto(s)
Anticuerpos/genética , Camelus/inmunología , Cadenas Pesadas de Inmunoglobulina/genética , Cadenas Pesadas de Inmunoglobulina/inmunología , Secuencia de Aminoácidos , Animales , Afinidad de Anticuerpos , Especificidad de Anticuerpos , Bacteriófagos/genética , Sitios de Unión de Anticuerpos , Clonación Molecular , Mapeo Epitopo , Biblioteca de Genes , Cadenas Pesadas de Inmunoglobulina/metabolismo , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/metabolismoRESUMEN
The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
Asunto(s)
Camelus/inmunología , Cadenas Pesadas de Inmunoglobulina/química , Región Variable de Inmunoglobulina/química , Secuencia de Aminoácidos , Animales , Sitios de Unión de Anticuerpos , Cristalografía por Rayos X , Humanos , Ratones , Datos de Secuencia Molecular , Muramidasa/química , Conformación Proteica , Pliegue de Proteína , Proteínas Recombinantes de Fusión , Alineación de SecuenciaRESUMEN
Virulence-associated plasmids pLT2, pIP1350, pIP1360 and pIP1370 coded for the fi+ character. These plasmids did not code for the synthesis of classical adhesins, aerobactin or colicins. Nor did they mediate resistance to the metals Hg, Te, As, Cd or flavomycin. Plasmids pLT2, pIP1350 and pIP1370 contained DNA sequences homologous to the incFIIA and incFIB replicons. Plasmid pIP1360 contained a single sequence homologous to incFIIA replicon.
Asunto(s)
ADN Bacteriano/genética , Plásmidos , Salmonella typhimurium/genética , Secuencia de Bases , Genotipo , Fenotipo , Replicón , Salmonella typhimurium/patogenicidad , Homología de Secuencia de Ácido Nucleico , VirulenciaRESUMEN
Mini-F is a segment of the conjugative plasmid F consisting of two origins of replication flanked by regulatory regions, which ensure a normal control of replication and partitioning. Adjacent to the ori-2 origin is a complex coding region that consists of the E gene overlapped by three open reading frames with the coding potential for 9000 Mr polypeptides here designated 9 kd-1, 9 kd-2 and 9 kd-3. In this paper, we show that open reading frame 9 kd-3 is preceded by active promoter and Shine-Dalgarno sequences. The E coding region specifies: an initiator of replication, which acts at the ori-2 site; a function that negatively regulates the expression of the E gene; and a function involved in mini-F copy number control. To assign one of these functions to one of the overlapping coding sequence, we have isolated, characterized and sequenced mutations mapping in the E coding region. In this paper, we analyse two mutations (cop5 and pla25) that abolish the repression of the E gene. As these mutations affect the primary structure of protein E itself but not the 9 kd polypeptides, we conclude that protein E takes part in the negative regulation of its own synthesis. In addition, the localization of the cop5 and pla25 mutations indicates that the carboxy-terminal end of the E protein is involved in the autorepression function. The cop5 mutation causes an eightfold increase of the mini-F copy number. The pla25 mutation leads to the inability of the derived mini-F plasmid to give rise to plasmid-harbouring bacteria. The ways in which the cop5 and pla25 mutations may lead to such phenotypes are discussed in relation to the different functions mapping in the E coding sequence.
Asunto(s)
Proteínas Bacterianas , Escherichia coli/genética , Factor F , Regulación de la Expresión Génica , Genes Bacterianos , Secuencia de Aminoácidos , Secuencia de Bases , ADN Bacteriano , Electroforesis en Gel de Agar , Focalización Isoeléctrica , Mutación , Biosíntesis de ProteínasRESUMEN
Properties of an inversion and a deletion mutant of B. subtilis phage SPP1 which arose during cloning are described. The results are related to the biology of this bacteriophage. In preceding communications from our laboratories (Heilmann and Reeve 1982, Behrens et al. 1983) we reported the properties of genetically engineered SPP1 bacteriophages, which could be used as cloning vehicles in B. subtilis. These phages contain a unique restriction site within a dispensable region of their genomes. In the course of cloning experiments using these phage vectors, we have occasionally observed the appearance of not only the original vector and desired hybrid phages, but also of SPP1 phages which had undergone extensive genomic rearrangements. Properties of two such phages, SPP1 inv1, which was found to contain a large inversion and of SPP1 delV, a deletion mutant, which defines an additional dispensable region of the SPP1 genome, are described in this communication.
Asunto(s)
Bacteriófagos/genética , Clonación Molecular , Bacillus subtilis , Deleción Cromosómica , Inversión Cromosómica , ADN Recombinante/análisis , ADN Viral/análisis , Genes Virales , Vectores Genéticos , MutaciónRESUMEN
In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). lambdaF recombinants were shown to be chimerae between the lambda and the F replicons. By genetical tests, we have demonstrated that both lambda and F replication mechanisms are functional: the lambdaF recombinant behaves as a non defective plaque forming phage on lambda sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the lambdaF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed.