Graded FGF activity patterns distinct cell types within the apical sensory organ of the sea anemone Nematostella vectensis.

Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each sensory unit has a defined architecture built from component cell types, including sensory cells, non-sensory support cells, and dedicated sensory neurons. Whether this characteristic cellular composition is present in the sensory organs of non-bilaterian animals is unknown. Here, we interrogate the cell type composition and gene regulatory networks controlling development of the larval apical sensory organ in the sea anemone Nematostella vectensis. Using single cell RNA sequencing and imaging approaches, we reveal two unique cell types in the Nematostella apical sensory organ, GABAergic sensory cells and a putative non-sensory support cell population. Further, we identify the paired-like (PRD) homeodomain gene prd146 as a specific sensory cell marker and show that Prd146+ sensory cells become post-mitotic after gastrulation. Genetic loss of function approaches show that Prd146 is essential for apical sensory organ development. Using a candidate gene knockdown approach, we place prd146 downstream of FGF signaling in the apical sensory organ gene regulatory network. Further, we demonstrate that an aboral FGF activity gradient coordinately regulates the specification of both sensory and support cells. Collectively, these experiments define the genetic basis for apical sensory organ development in a non-bilaterian animal and reveal an unanticipated degree of complexity in a prototypic sensory structure.

An Inorganic Chemistry Laboratory Technique Course using Scaffolded, Inquiry-Based Laboratories and Project-Based Learning.

To enhance students' learning and help them understand the whole picture of the field of inorganic chemistry, an inorganic laboratory technique course was designed that uses scaffolded, inquiry-based lab experiments and project-based learning. The scaffolded, inquiry-based laboratories taught in the first 8 weeks of the course helped students better understand the aim of each lab and how to apply each lab technique to a bigger research project. The laboratory experiments also included opportunities for cooperative and collaborative learning through student group work and feedback. To further develop students' independent research skills, we implemented project-based learning in the second part of the course (last 4 weeks), in which students develop a research proposal based on independent literature research and the laboratory techniques they learned from the course. Pilot data suggest that the course helped improve students' interest in inorganic chemistry, science self-efficacy, and science identity. Additionally, students reported that both the scaffolded, inquiry-based laboratories and the project-based learning module enhanced their problem-solving and critical thinking skills.

Students' Experiences and Perceptions of the Scientific Research Culture after Participating in Different Course-Based Undergraduate Research Experience Models.

Undergraduate students interact with the culture of scientific research when they participate in direct mentorship experiences and laboratory courses such as course-based undergraduate research experiences (CUREs). Much work has been done to explore how CUREs impact the interest, motivation, and retention of undergraduate students in science. However, little work has been done exploring students' experiences and perceptions of the culture of scientific research in the CURE context, and how different CURE models representing different subfields of science impact these experiences and perceptions. This study explored which cultural aspects of scientific research students experienced after participating in a CURE and whether their perceptions of those cultural aspects differed based on students' participation in a bench-based or computer-based research project. Students discussed the Practices and Norms/Expectations of scientific research most frequently. Students in the bench-based and computer-based project areas mentioned different cultural aspects as important to their experiences. Bench-based and computational students also had different perceptions of some of the same cultural aspects, including Teamwork, Freedom & Independence, and Persistence & Resilience. These results suggest that different CURE models differentially impact students' experiences and perceptions of the culture of scientific research, which has implications for examining how students move into scientific research.

Improving Students' Understanding of Biological Variation in Experimental Design and Analysis through a Short Model-Based Curricular Intervention.

When conducting biological investigations, experts constantly integrate their conceptual and quantitative understanding of variation with the design and analysis of the investigation. This process is difficult for students, because curricula often treat these concepts as separate components. This study describes the effect of a curricular intervention aimed at improving students' conceptual and quantitative understanding of variation in the context of experimental design and analysis. A model-based intervention curriculum consisting of five short modules was implemented in an introductory biology laboratory course. All students received the regular laboratory curriculum, and half of the students also received the Intervention curriculum. Students' understanding of variation was assessed using a published 16-question multiple-choice instrument designed and validated by the research team. Students were assessed before and after the intervention was implemented, and normalized gain scores were calculated. Students who received the intervention showed significantly higher normalized gains than students who did not receive the intervention. This effect was not influenced by students' gender or exposure to prior statistics courses and persisted into and through the following semester's laboratory course. These results provide support for the use of model-based approaches to improve students' understanding of biological variation in experimental design and analysis.

Teasing apart the impacts of curriculum and professional development on teaching assistants' teaching practices.

Teaching assistants (TAs) often lead courses using curricula they did not design. Therefore, examining how curriculum and professional development (PD) interact to influence TAs' teaching practices is critical. This study describes the effects of a curriculum and PD intervention in two contexts: when TAs are teaching curriculum that is explicitly linked to PD, and when teaching curriculum that is not linked to PD. The Intervention curriculum featured structured opportunities for reform-oriented teaching practices. The Intervention PD was situated in the context of these specific curriculum activities and modelled the desired teaching practices. TAs that participated in the intervention implemented more student-centered teaching practices than TAs that did not participate in the intervention, even when teaching curriculum that was not designed to be student-centered and was not linked to PD. A linear model of TAs' teaching practices that included PD type, task cognitive demand and curriculum type indicates that cognitive demand has the largest relationship with teaching practices, followed by PD type. These results have implications for policy. They suggest that investment in curriculum-linked TA PD can be effective even when teaching curricula that is not linked to PD. Additionally, investment in development of higher-cognitive-demand tasks may be an effective strategy to support implementation of student-centered practices.

Camouflaging in Autistic and Non-autistic Adolescents in the Modern Context of Social Media.

Camouflaging is described as a set of strategies used to prevent others from noticing one's social difficulties. Research indicates heightened levels of camouflaging behaviours in the adult autistic population. To extend understanding of camouflaging in adolescents, this mixed-methods study explored camouflaging behaviours in offline and online contexts with 40 autistic and 158 non-autistic adolescents. At the quantitative phase, participants completed measures of camouflaging behaviours (online vs offline) and measures of social media use. Following this, six autistic adolescents participated in semi-structured interviews. Findings indicate that in the offline context, autistic adolescents camouflage more than non-autistic adolescents. Online, autistic participants camouflage less than they do offline, and females camouflage more than males. Implications for research and theory are discussed.

Paired Multiple-Choice Questions Reveal Students' Incomplete Statistical Thinking about Variation during Data Analysis.

Biologists consider variability during biological investigations. A robust quantitative understanding of variability is particularly important during data analysis, where statistics are used to quantify variation and draw conclusions about phenomena while accounting for variation. Many students struggle to correctly apply a quantitative understanding of variation to statistically analyze data. We present quantitative and qualitative analyses of introductory biology students' responses on two pairs of multiple-choice questions querying two concepts related to the quantitative analysis of variation. More students correctly identify a mathematical expression of variation than correctly interpret it. Many students correctly interpret a nonsignificant p-value in the context of a very small sample size, but fewer students do so in the context of a large sample size. These results imply that many students have an incomplete quantitative understanding of variation. These findings suggest that instruction focusing on conceptual understanding, not procedural problem solving, may elevate students' quantitative understanding of variation.

Development of a Framework for the Culture of Scientific Research.

Scientific research has a culture that can be challenging to enter. Different aspects of this culture may act as barriers or entry points for different people. Recognition of these barriers and entry points requires identifying aspects of the culture of scientific research and synthesizing them into a single, descriptive framework. A systematic literature review encompassing a two-pronged search strategy, descriptive mapping of ideas, and consensus building, was performed to identify aspects of scientific research culture. This resulted in the Culture of Scientific Research (CSR) Framework, composed of 31 cultural aspects categorized as either Practices, Norms/Expectations, or Values/Beliefs. Additional evidence of validity was collected through a survey that asked biological researchers to indicate which aspects in the framework were relevant to their experiences of research. The majority of survey respondents (n = 161) perceived the 31 aspects in the CSR Framework as relevant to biological research. This framework provides a consistent structure for describing the experiences of people engaging with the culture of scientific research. The literature review included literature from multiple disciplines, so the CSR Framework should be broadly applicable. Future applications of the CSR Framework include identifying possible barriers and entry points experienced by groups currently underrepresented in scientific research.

Development of the Biological Variation In Experimental Design And Analysis (BioVEDA) assessment.

Variation is an important concept that underlies experimental design and data analysis. Incomplete understanding of variation can preclude students from designing experiments that adequately manage organismal and experimental variation, and from accurately conducting and interpreting statistical analyses of data. Because of the lack of assessment instruments that measure students' ideas about variation in the context of biological investigations, we developed the Biological Variation in Experimental Design and Analysis (BioVEDA) assessment. Psychometric analyses indicate that BioVEDA assessment scores are reliable/precise. We provide evidence that the BioVEDA instrument can be used to evaluate students' understanding of biological variation in the context of experimental design and analysis relative to other students and to their prior scores.

Activating children's thinking skills (ACTS): the effects of an infusion approach to teaching thinking in primary schools.

BACKGROUND: Recent interest in the teaching of thinking skills within education has led to an increase in thinking skills packages available to schools. However many of these are not based on scientific evaluation (DfEE, 1999). This paper endeavours to examine the effectiveness of one approach, that of infusion, to teaching thinking. AIMS: To investigate the impact of an infusion methodology, activating children's thinking skills (ACTS), on the cognitive, social, and emotional development of children in Year 4-6 in primary schools. This is a sister project to research being conducted in Northern Ireland (McGuinness, 2006). SAMPLE: The study involved 404 children from 8 primary schools in one local authority. These were divided into 160 in the experimental group and 244 in the waiting list control group. METHODS: A quasi-experimental design was used with pre-, post-, and delayed post-tests to ascertain changes in children's cognitive abilities, self-perceptions, and social/behavioural skills using quantitative measures. In addition qualitative techniques were used with pupils and teachers to evaluate effectiveness. RESULTS: The experimental group made significantly greater gains in cognitive ability skills over a 2 year period compared to the waiting list control. Qualitative data demonstrated a positive impact on children's social and emotional development. In addition teacher professional development was reported to be enhanced. CONCLUSIONS: This research indicated that children's cognitive abilities can be developed following a 2 year period of the ACTS infusion intervention. While some positive effects were evidenced on the social and emotional development of children, further study will be necessary to examine these in more detail.