Asunto(s)
Automatización de Laboratorios , Virus BK/genética , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/normas , Infecciones por Polyomavirus/diagnóstico , Infecciones Tumorales por Virus/diagnóstico , Automatización de Laboratorios/métodos , Automatización de Laboratorios/normas , Trasplante de Médula Ósea/efectos adversos , Trasplante de Médula Ósea/normas , ADN Viral/análisis , ADN Viral/genética , Humanos , Trasplante de Riñón/efectos adversos , Trasplante de Riñón/normas , Tipificación Molecular/métodos , Tipificación Molecular/normas , Seguridad del Paciente/normas , Reacción en Cadena de la Polimerasa/instrumentación , Infecciones por Polyomavirus/transmisión , Infecciones por Polyomavirus/virología , Guías de Práctica Clínica como Asunto/normas , Estándares de Referencia , Receptores de Trasplantes , Infecciones Tumorales por Virus/transmisión , Infecciones Tumorales por Virus/virología , Carga Viral , Organización Mundial de la SaludRESUMEN
We here report the case of a 30-year old man with a history of ulcerative colitis, who presented clinical and biological features compatible with a viral hepatitis. Initial serological results revealed the presence of IgM antibodies against many viruses, and the most likely diagnosis was viral hepatitis A. However, further investigations were performed and concluded to cytomegalovirus primary infection.
Asunto(s)
Colitis Ulcerosa , Infecciones por Citomegalovirus , Citomegalovirus , Inmunoglobulina M/sangre , Dolor Abdominal , Adulto , Anticuerpos Antivirales/inmunología , Colitis Ulcerosa/sangre , Colitis Ulcerosa/complicaciones , Colitis Ulcerosa/inmunología , Colitis Ulcerosa/fisiopatología , Infecciones por Citomegalovirus/complicaciones , Infecciones por Citomegalovirus/diagnóstico , Diagnóstico Diferencial , Fiebre , Hepatitis A/complicaciones , Hepatitis A/diagnóstico , Humanos , Inmunoglobulina M/inmunología , Masculino , SerologíaRESUMEN
We here report the case of a 52-year old man who presented hepatitis with hemophagocytic syndrome triggered by herpes simplex 1 (HSV-1) primary infection. A high-level viremia was observed, and HSV-1 DNA remained detectable in blood for a long time after patient's recovery.
Asunto(s)
ADN Viral/sangre , Hepatitis Viral Humana/virología , Herpes Simple/virología , Herpesvirus Humano 1/genética , Linfohistiocitosis Hemofagocítica/etiología , Aciclovir/uso terapéutico , Etopósido/uso terapéutico , Hepatitis Viral Humana/sangre , Hepatitis Viral Humana/tratamiento farmacológico , Herpes Simple/sangre , Herpes Simple/complicaciones , Herpes Simple/tratamiento farmacológico , Herpesvirus Humano 1/efectos de los fármacos , Humanos , Inmunoglobulinas Intravenosas/uso terapéutico , Linfohistiocitosis Hemofagocítica/sangre , Linfohistiocitosis Hemofagocítica/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Resultado del Tratamiento , Carga Viral/efectos de los fármacosAsunto(s)
Pruebas Diagnósticas de Rutina/métodos , Encefalitis Viral/diagnóstico , Encefalitis Viral/patología , Infecciones por Parvoviridae/diagnóstico , Infecciones por Parvoviridae/patología , Parvovirus B19 Humano/aislamiento & purificación , Adolescente , Diagnóstico Diferencial , Humanos , MasculinoRESUMEN
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of virus respiratory syncytial virus (RSV), human herpes virus 6 (HHV6) or adenovirus allogeneic Stem Cell Transplantation.
Asunto(s)
Infecciones por Adenoviridae/terapia , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Infecciones por Virus Sincitial Respiratorio/terapia , Infecciones por Roseolovirus/terapia , Activación Viral/fisiología , Adenoviridae/fisiología , Infecciones por Adenoviridae/epidemiología , Infecciones por Adenoviridae/etiología , Consenso , Selección de Donante/normas , Trasplante de Células Madre Hematopoyéticas/normas , Trasplante de Células Madre Hematopoyéticas/estadística & datos numéricos , Herpesvirus Humano 6/fisiología , Humanos , Terapia de Inmunosupresión/normas , Terapia de Inmunosupresión/estadística & datos numéricos , Infecciones por Virus Sincitial Respiratorio/epidemiología , Infecciones por Virus Sincitial Respiratorio/etiología , Virus Sincitiales Respiratorios/fisiología , Infecciones por Roseolovirus/epidemiología , Infecciones por Roseolovirus/etiología , Trasplante HomólogoRESUMEN
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of pre-transplant donor's cytomegalovirus, Epstein-Barr virus, Toxoplasma gondii, or syphilis IgM positive serology test.
Asunto(s)
Infecciones por Citomegalovirus/diagnóstico , Selección de Donante/normas , Infecciones por Virus de Epstein-Barr/diagnóstico , Trasplante de Células Madre Hematopoyéticas/normas , Hallazgos Incidentales , Sífilis/diagnóstico , Toxoplasmosis/diagnóstico , Donantes de Sangre , Consenso , Citomegalovirus/aislamiento & purificación , Infecciones por Citomegalovirus/sangre , Infecciones por Virus de Epstein-Barr/sangre , Francia , Conocimientos, Actitudes y Práctica en Salud , Herpesvirus Humano 4/aislamiento & purificación , Humanos , Inmunoglobulina M/sangre , Sífilis/sangre , Sífilis/inmunología , Toxoplasma/aislamiento & purificación , Toxoplasmosis/sangre , Trasplante HomólogoRESUMEN
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of common issues related to the donor: pre-transplant pregnancy and monoclonal gammopathy.
Asunto(s)
Donantes de Sangre , Selección de Donante/normas , Conocimientos, Actitudes y Práctica en Salud , Trasplante de Células Madre Hematopoyéticas/normas , Hallazgos Incidentales , Paraproteinemias/diagnóstico , Pruebas de Embarazo , Consenso , Femenino , Edad Gestacional , Humanos , Paraproteinemias/sangre , Embarazo/sangre , Complicaciones del Embarazo/sangre , Complicaciones del Embarazo/prevención & controlAsunto(s)
Infecciones por Citomegalovirus/diagnóstico , Infecciones por Citomegalovirus/patología , Citomegalovirus/inmunología , Fiebre de Origen Desconocido/diagnóstico , Leucocitos Mononucleares , Adulto , Anticuerpos Antivirales/sangre , Reacciones Cruzadas , Citomegalovirus/fisiología , Diagnóstico Diferencial , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Masculino , Activación ViralRESUMEN
BACKGROUND: High-risk HPV (HR-HPV) are associated with the development of cervical cancer, the most common cancer in women in developing countries. Reliable diagnosis of HR-HPV infection combined with simple procedures to collect and store biological samples, could improve primary screening programs and vaccine strategies in these areas. OBJECTIVE: To evaluate HR-HPV detection in conventional and dried samples. STUDY DESIGN: The presence of HR-HPV in 31 women in Republic of Congo (Central Africa) has been investigated by using standard cervical samples and dried cervical samples collected on filter paper and vaginal tampons. The detection of HPV DNA was performed in the Laboratory of virology in Lille (France) by using Hybrid capture 2 and HPV 16/18/45 Probe Set Test. RESULTS: 22 standard samples were found positive for the detection of HR-HPV (71%). HPV 16/18/45 was displayed in 15 out of 22 samples positive for HR-HPV (68%). The correlations between HPV detection by using standard samples and samples dried on filter paper and dried tampons were 90.3% (kappa = 0.77) and 80.6% (kappa = 0.5) respectively. The sensitivity and the specificity of HPV detection reached 91% and 89%, respectively, with samples dried on filter paper and were 86% and 67%, respectively, for dried tampons compared with standard samples. CONCLUSION: Dried cervical samples and dried vaginal tampons can represent an alternative to conventional sampling to reduce barriers to large screening programs in developing countries and to facilitate storage and transport to reference centers.
Asunto(s)
Cuello del Útero/virología , Filtración/métodos , Productos para la Higiene Menstrual/virología , Papillomaviridae/genética , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Manejo de Especímenes/métodos , Neoplasias del Cuello Uterino/diagnóstico , Congo , ADN Viral/análisis , ADN Viral/genética , Desecación , Femenino , Papillomavirus Humano 16/genética , Papillomavirus Humano 16/aislamiento & purificación , Papillomavirus Humano 18/genética , Papillomavirus Humano 18/aislamiento & purificación , Humanos , Papillomaviridae/clasificación , Infecciones por Papillomavirus/epidemiología , Infecciones por Papillomavirus/virología , Riesgo , Sensibilidad y Especificidad , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/virologíaRESUMEN
The respiratory syncytial virus (RSV) is known as a major cause of respiratory infections and nosocomial diseases. Testing this virus is rather difficult due to the problems encountered in producing it at a high titer without using any purification method. A RSV isolate which replicates to high level on a Hep-2 cell line with an infectious titer of at least 10(7)TCID(50)mL(-1) in culture supernatant fluids has been identified. Thanks to this isolate, the virucidal effects of two products, a hand rub solution and a surface disinfectant, were conveniently tested according to the EN 14476:2007-02 procedure.
Asunto(s)
Antivirales/farmacología , Desinfectantes/farmacología , Virus Sincitiales Respiratorios/aislamiento & purificación , Línea Celular , Humanos , Pruebas de Sensibilidad Microbiana , Virus Sincitiales Respiratorios/efectos de los fármacos , Virus Sincitiales Respiratorios/fisiología , Cultivo de VirusRESUMEN
INTRODUCTION: There are a few reports only on cytomegalovirus (CMV) reactivation in lupus. Diagnosis of this infection is difficult and can be associated with of a poor outcome. We report three cases of infection with CMV that occurred in patients with lupus and review the literature. CASE REPORTS: The three reported patients presented with fever, polyarthritis, myocarditis and enteritis. Lupus was longstanding and the patients were receiving corticosteroids or cyclophosphamide. There was no major CD4 lymphopenia. The diagnosis was obtained with the presence of antigenemia pp65. The outcome was favorable with antiviral therapy in two patients, while the remaining patient died. In the English literature, pulmonary and intestinal involvement seem frequent, and associated with poor prognosis. CONCLUSION: In systemic lupus CMV infection is often serious and difficult to diagnose. Risk factors, treatment and prophylaxis remain to be evaluated in this population. The incidence of this infection could increase among patients receiving a biotherapy.
Asunto(s)
Infecciones por Citomegalovirus/diagnóstico , Lupus Eritematoso Sistémico/complicaciones , Corticoesteroides/uso terapéutico , Adulto , Antivirales/uso terapéutico , Ciclofosfamida/uso terapéutico , Infecciones por Citomegalovirus/complicaciones , Infecciones por Citomegalovirus/tratamiento farmacológico , Infecciones por Citomegalovirus/epidemiología , Femenino , Humanos , Inmunosupresores/uso terapéutico , Incidencia , Lupus Eritematoso Sistémico/tratamiento farmacológico , Persona de Mediana Edad , PronósticoRESUMEN
The main predictor of outcomes in herpes simplex virus (HSV) encephalitis (HSE) is the delay between hospital admission and initiation of acyclovir. In this study, factors associated with late initiation of acyclovir were identified. The study included adults from northern France whose cerebrospinal fluid (CSF) was positive for HSV by PCR. Late initiation of acyclovir was defined as a delay of >1 day from hospital admission. In total, 184 patients were retrospectively enrolled from January 1991 to December 2002. The median age was 60 years (range: 17-91), and 102 (55.4%) were male. Acyclovir was initiated >1 day after hospital admission in 68 patients (37.0%). According to multivariate analysis, independent risk factors for late initiation of acyclovir were severe underlying disease (Knaus score >or=C) (OR 4.1; 95% CI 1.5-11.7); alcohol abuse (OR 3.4; 95% CI 1.3-8.9); and a delay of >1 day from admission to first brain imaging (OR 8.4; 95% CI 3.9-18.0). In addition, univariate analysis suggested an association between a finding of <10 leukocytes/mm(3) in CSF at admission (OR 2.5; 95% CI 0.7-5.8). These characteristics were found in 26 (14.1%), 23 (12.5%), 66 (35.9%) and 27 (14.7%) patients, respectively. One risk factor was identified in 109 (59.2%) patients, two in 29 (15.8%), and three in six (3.3%). Patients with HSE often present with severe underlying disease, chronic alcohol abuse, or atypical CSF findings, and such factors should not be allowed to delay diagnosis and administration of acyclovir.
Asunto(s)
Aciclovir/uso terapéutico , Antivirales/uso terapéutico , Encefalitis Viral/tratamiento farmacológico , Encefalitis Viral/virología , Herpes Simple/complicaciones , Herpes Simple/tratamiento farmacológico , Simplexvirus/aislamiento & purificación , Factores de Tiempo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Líquido Cefalorraquídeo/virología , Femenino , Francia , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa/métodos , Estudios Retrospectivos , Resultado del Tratamiento , Adulto JovenRESUMEN
Human herpesvirus-6 (HHV-6) has been identified as the causal agent of exanthema subitum in early childhood (also called sixth disease or roseola), a mononucleosis-like disease in adults, and as an opportunistic pathogen in transplant recipients. In the latter setting, most infections are caused by reactivation of the latent virus in the recipient and generally have a paucisymptomatic course. Only limited data on HHV-6 infection are available for liver transplant recipients. Herein we have reported a case of fatal hemophagocytic syndrome related to HHV-6 reactivation 2 weeks after liver transplantation (LT). This case suggests that this virus may be a serious and potentially life-threatening pathogen following LT.
Asunto(s)
Herpesvirus Humano 6 , Trasplante de Riñón/efectos adversos , Trasplante de Hígado/efectos adversos , Linfohistiocitosis Hemofagocítica/virología , Enfermedades Renales Poliquísticas/cirugía , Infecciones por Roseolovirus/complicaciones , Infecciones por Citomegalovirus/complicaciones , Resultado Fatal , Femenino , Humanos , Persona de Mediana Edad , RecurrenciaRESUMEN
Influenza viruses continue to be accountable for winter outbreaks. The potential for developing complications is higher in certain risk groups, such as children, the elderly and individuals with chronic medical conditions. We have presented a duplex real-time RT-PCR for detection of influenza A and B. The sensibility of our method was estimated at 0.01TCID(50)/ml for detection of influenza A and 0.1TCID(50)/ml for influenza B. Any other viruses with respiratory tract tropism were detected with our method. After that, we tested our duplex RT-PCR on 119 samples (nasal aspirates and liquids of broncho-alveolar washes) collected in the CHRU of Lille between November 2005 and January 2006 from patients aged one month to 81 years. Conventional methods such as direct immunofluorescence (IF) assay and/or cell culture were applied on these samples. Four samples were positive for influenza A virus detection and, in particular, one liquid of broncho-alveolar wash for which the direct IF assay was negative. Thus, our method is adapted to diagnosis of flu infections. Nevertheless, our duplex RT-PCR will be tested during a flu outbreak.
Asunto(s)
Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza B/aislamiento & purificación , Gripe Humana/virología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Líquido del Lavado Bronquioalveolar/virología , Niño , Preescolar , Sistemas de Computación , Femenino , Técnica del Anticuerpo Fluorescente Directa , Francia/epidemiología , Hospitales Universitarios , Humanos , Lactante , Recién Nacido , Virus de la Influenza A/genética , Virus de la Influenza B/genética , Gripe Humana/epidemiología , Masculino , Persona de Mediana Edad , Cavidad Nasal/virología , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Adulto JovenRESUMEN
First seen in Ghana and Indonesia in the early 70's, acute haemorrhagic conjunctivitis or "Apollo 11" disease is an eye infection caused by Enterovirus type 70 (EV70). The disease appeared to be a highly contagious conjunctivitis which spread rapidly all over the world. EV70 has been considered as an emerging virus and was classified as a new Enterovirus. No human or animal virus genetically similar to EV70 was known before the sudden outcome of the disease in Ghana, West Africa. EV70 appeared as a pretty demonstrative example of virus emergence and virus spreading. Studies of virus genetic mutations emphasized the variations of RNA virus within a short time period. The current review presents the EV70 infection and the genetic profile of the virus from its emergence to nowadays.
Asunto(s)
Conjuntivitis Hemorrágica Aguda , Enterovirus Humano D , Infecciones por Enterovirus , África/epidemiología , Asia/epidemiología , Conjuntivitis Hemorrágica Aguda/diagnóstico , Conjuntivitis Hemorrágica Aguda/epidemiología , Conjuntivitis Hemorrágica Aguda/virología , Diagnóstico Diferencial , Enterovirus Humano D/genética , Enterovirus Humano D/aislamiento & purificación , Enterovirus Humano D/fisiología , Infecciones por Enterovirus/diagnóstico , Infecciones por Enterovirus/epidemiología , Infecciones por Enterovirus/virología , Europa (Continente)/epidemiología , Genes Virales/genética , Variación Genética , Humanos , Mutación , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
AIMS: Vanadium chloroperoxidase and its directed evolution mutant P395D/L241V/T343A were investigated for their antibacterial and antiviral potential at slightly alkaline pH and at a H(2)O(2) concentration that is low compared to current nonenzymatic formulations. METHODS AND RESULTS: Two bacteria (the Gram-negative Pseudomonas aeruginosa and the Gram-positive Staphylococcus aureus) and two viruses (the enveloped Herpes Simplex Virus and the nonenveloped Coxsackievirus B4) were incubated with the P395D/L241V/T343A mutant, 10 mmol l(-1) H(2)O(2) and 100 mmol l(-1) Br(-) at pH 8. Strong microbial reduction was observed and bactericidal and virucidal activities of the mutant were three to six orders of magnitude higher than for the wild-type enzyme. CONCLUSIONS: The P395D/L241V/T343A mutant of vanadium chloroperoxidase has a broad antimicrobial activity at alkaline conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: For many disinfection formulations, antimicrobial activity at slightly alkaline pH values is required. To date, only the wild-type vanadium chloroperoxidase has been studied for its antibacterial activity, and only at acidic to neutral pH values. Its antiviral activity (e.g. useful for the cleaning of medical equipment) was not studied before. The observed activity for the alkalophilic P395D/L241V/T343A mutant is an important step forward in the application of this robust enzyme as a component in disinfection formulations.
Asunto(s)
Antiinfecciosos/farmacología , Cloruro Peroxidasa/farmacología , Desinfección/métodos , Antibacterianos/farmacología , Antivirales/farmacología , Cloruro Peroxidasa/genética , Evolución Molecular Dirigida , Enterovirus/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Mutagénesis , Pseudomonas/efectos de los fármacos , Simplexvirus/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Inactivación de VirusRESUMEN
Hepatitis C virus (HCV) is an enveloped virus and encodes two envelope glycoproteins, E1 and E2. E1 and E2 are transmembrane type I proteins with a N-terminal ectodomain and C-terminal anchor. During their synthesis, E1 and E2 ectodomains are targeted in the endoplasmic reticulum lumen where they are modified by N-linked glycosylation. After their synthesis, E1 and E2 assemble as a non-covalent heterodimer. The N-linked glycosylation is based on the recognition of specific asparagine residue in the context of the consensus sequence Asn-X-Ser/Thr. E1 contains potentially 4 or 5 N-linked glycosylation sites and E2 up to 11. Recent data indicated that some glycans of glycoproteins E1 and E2 play a major role in protein folding and heterodimer formation. Some N-linked glycans of E2 were involved in interactions with CD81, a putative cellular receptor for HCV. It appeared that N-linked glycans of E1 and E2 played an important role of in the viral entry.
Asunto(s)
Hepacivirus/metabolismo , Polisacáridos/fisiología , Proteínas del Envoltorio Viral/fisiología , Glicosilación , Hepacivirus/patogenicidadAsunto(s)
Herpes Simple/complicaciones , Herpesvirus Humano 1/aislamiento & purificación , Hidropesía Fetal/virología , Complicaciones Infecciosas del Embarazo/virología , Adulto , ADN Viral/análisis , Resultado Fatal , Femenino , Sangre Fetal/virología , Edad Gestacional , Herpes Simple/patología , Herpesvirus Humano 1/genética , Herpesvirus Humano 1/inmunología , Humanos , Hidropesía Fetal/patología , Embarazo , Complicaciones Infecciosas del Embarazo/patología , MortinatoRESUMEN
We report a case of a 15-year-old young man who was admitted for an acute hepatitis. Virological assessment showed both IgM anti-EBV and IgM anti-hepatitis A. IgG anti-EBNA and clinical history allowed to rule out the hypothesis of a recent EBV infection and confirmed the diagnosis of acute hepatitis A infection.
Asunto(s)
Virus de la Hepatitis A/inmunología , Hepatitis A/diagnóstico , Herpesvirus Humano 4/inmunología , Inmunoglobulina M/análisis , Enfermedad Aguda , Adolescente , Urgencias Médicas , Estudios de Seguimiento , Hepatitis A/inmunología , Humanos , Unidades de Cuidados Intensivos , Masculino , Factores de TiempoRESUMEN
Parvovirus B19 classically causes erythema infectiosum in children, febrile arthralgia or acute erythroblastopenia in adult. The clinical spectrum of adult primary infection is sometimes misleading. We report an observation of an acute rheumatoid-like arthritis following primary parvovirus B19 infection in a 42-year-old woman.