From Aedes to Zeugodacus: a review of dipteran body coloration studies regarding evolutionary developmental biology, pest control, and species discovery.

Over the past two decades, evo-devo (evolution of development) studies have elucidated genetic mechanisms underlying novel dipteran body color patterns. Here we review the most recent developments, which show some departure from the model organism Drosophila melanogaster, leading the field into the investigation of more complex color patterns. We also discuss how the robust application of transgenic techniques has facilitated the study of many non-model pest species. Furthermore, we see that subtle pigmentation differences guide the discovery and description of new dipterans. Therefore, we argue that the existence of new field guides and the prevalence of pigmentation studies in non-model flies will enable scientists to adopt uninvestigated species into the lab, allowing them to study novel morphologies.

The modular expression patterns of three pigmentation genes prefigure unique abdominal morphologies seen among three Drosophila species.

To understand how novel animal body colorations emerged, one needs to ask how the development of color patterns differs among closely related species. Here we examine three species of fruit flies - Drosophila guttifera (D. guttifera), D. palustris, and D. subpalustris - displaying a varying number of abdominal spot rows. Through in situ hybridization experiments, we examine the mRNA expression patterns for the pigmentation genes Dopa decarboxylase (Ddc), tan (t), and yellow (y) during pupal development. Our results show that Ddc, t, and y are co-expressed in modular, identical patterns, each foreshadowing the adult abdominal spots in D. guttifera, D. palustris, and D. subpalustris. We suggest that differences in the expression patterns of these three genes partially underlie the morphological diversity of the quinaria species group.