Surface wave investigation and phenomenological analysis: Application on in vivo human cutaneous tissue.

The wave phenomenon in free surface media stems from the propagation of mode grouping. Due to the nature of propagation in a given medium, this phenomenon expresses different types of dependence on the medium's properties and represents its mechanical admittance. In contrast with body wave propagation, dependencies related to surface propagation in a medium can be described by spatial-temporal characteristics. These characteristics can be obtained by performing appropriate experiments and do not require prior knowledge of the physical properties of the medium. In this study, we propose an original surface wave investigation and a phenomenological analysis approach adapted to the mechano-bio-structural states evaluation of in vivo human skin. Two objectives are sought with the method proposed: the first concerns the development of a non-invasive device for generating and tracking surface waves in human skin called Free-Skin-Surface-Wave (FSSW); the second concerns the adaptation of the Multi-Chanel Analysis of Surface Waves (MASW) method to evaluate the mechano-bio-structural states of human cutaneous tissue in vivo on the basis of the propagating phenomena observed. As an illustration of the proposed method application, we have done an in vivo evaluation, on intern-forearm of female volunteers population. In addition, we proposed a study of the aging effect and a comparison with ultrasound B-Mode technique, to validate the method sensitivity to follow the mechano-morphological properties of the in vivo human skin. In this study, our medium of application was human skin in vivo, but it is conceivable to extend this application to other soft biological media.

Mechanical and topographical anisotropy for human skin: Ageing effect.

Skin ageing is a complex process which strongly impacts the three skin layers (epidermis, dermis, hypodermis) both functionally and structurally. Of particular interest are the effects of ageing on the dermis biomechanics and how this evolution can impact the reorganization of the cutaneous lines which compose the skin relief. It has been argued that the skin relief could reflect the underlying mechanical condition of the skin. Nevertheless, there is not yet conclusive evidence of the existence of such a link. This work aims at experimentally studying, in vivo, the correlation between the anisotropy of human skin biomechanics and skin topography as a function of ageing. The study was conducted on a panel of 20 men divided into 4 groups according to age (from 23 to 64 years old). The measurements were performed on the right volar forearm of each volunteer. For the biomechanical measurements, an innovative contactless bio-rheometer was developed. It allows access to the mechanical behaviour of the skin in several directions. This device generates an air blast without any contact with the skin area and measures its dynamic response (evaluation of speed of wave propagation) with a linear laser. Moreover, a turntable enables measurements to be made in different angular directions. To analyse the topography of skin relief, we proposed a new method, based on watershed and linear radon transformations. First, an optical analysis of a replica of the skin relief is performed. Then, from the skin image obtained, the density of the cutaneous lines is calculated in different directions using watersheld transformation. The orientation of the detected lines is then estimated with an algorithm based on linear radon transformation. The results observed show a good correlation between the skin relief and the mechanical properties of the skin all along the ageing process. For both topography and mechanical properties, there is a transition from an almost isotropic mechanical behaviour to an anisotropic one as a function of ageing process. Thus, we might conclude that the skin relief reflects the underlying mechanical conditions of the skin.

Biophysical properties of the human finger for touch comprehension: influences of ageing and gender.

The human finger plays an extremely important role in tactile perception, but little is known about how age and gender affect its biophysical properties and their role in tactile perception. We combined studies on contact characteristics, mechanical properties and surface topography to understand age and gender effects on the human finger. The values obtained regarding contact characteristics (i.e. adhesive force) were significantly higher for women than for men. As for mechanical properties (i.e. Young's modulus E), a significant and positive correlation with age was observed and found to be higher for women. A positive correlation was observed between age and the arithmetic mean of surface roughness for men. However, an inverse age effect was highlighted for women. The age and gender effects obtained have never been reported previously in the literature. These results open new perspectives for understanding the weakening of tactile perception across ages and how it differs between men and women.

Elastic wave induced by friction as a signature of human skin ageing and gender effect.

BACKGROUND: In this work, we propose an innovative approach based on a rotary tribometer coupled with laser velocimetry for measuring the elastic wave propagation on the skin. METHODS: The method is based on a dynamic contact with the control of the normal force (Fn ), the contact length and speed. During the test a quantification of the friction force is produced. The elastic wave generated by friction is measured at the surface of the skin 35 mm from the source of friction exciter. In order to quantify the spectral range and the energy property of the wave generated, we have used laser velocimetry whose spot laser diameter is 120 µm, which samples the elastic wave propagation at a frequency which may reach 100 kHz. In this configuration, the speaker is the friction exciter and the listener the laser velocimetry. In order to perform non-invasive friction tests, the normal stress has been set to 0.3 N and the rotary velocity to 3 revolutions per second, which involves a sliding velocity of 63 mm/s. RESULTS AND CONCLUSIONS: This newly developed innovative tribometer has been used for the analysis of the elastic wave propagation induced by friction on human skin during chronological ageing and gender effect. Measurements in vivo have been made on 60 healthy men and women volunteers, aged from 25 to 70. The results concerning the energy of the elastic wave signature induced by friction show a clear difference between the younger and older groups in the range of a low band of frequencies (0-200 Hz). The gender effect was marked by a 20% decrease in the energy of elastic wave propagation in the female group.

Effect of water desorption on the rheology and dynamic response of human hair to a non-contact impact.

Human hair is a non-homogeneous complex material made of keratin fibers oriented along the longitudinal axis which offer anisotropic mechanical properties. Nowadays, it is possible to measure the mechanical properties of hairs with the classical tests, but most often, these tests are destructive and make hard to measure the influence of some external factors or treatments on the behavior of a same hair fiber. In the current paper, vibrations induced by a non-contact impact have been utilized as a representative response of the mechanical behavior of hair. The characteristics of the vibratory response allow measuring the variation in the mechanical properties and the instantaneous effect of an external factor on the properties of a same sample. First, load relaxation tests have been performed on hair samples after moisturization and for different times of an air-drying process in order to characterize the change in the visco-elastic behavior of hair during the water desorption. Other hair samples have been tested with our non-contact impact and vibration technique in order to observe the change in the vibratory response during the water desorption. The vibratory response has then been correlated to the mechanical properties of the hair fiber.