Hamster cheek pouch carcinoma: effect of incision and cortisone on growth, invasion, and metastasis.

After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies performed on tumors in their pouches, one of these also received injections of cortisone throughout the 20-week experimental period, and a fourth group received cortisone only. The wet weights of the cancerous cheek pouches were determined, and the submandibular and parotid salivary glands with associated cervical lymph nodes, the lungs, and the liver were examined with light microscopy. The cancerous pouches of the animals that received cortisone weighed significantly less than those of animals that received no cortisone but had incisional biopsies of the tumors. There was no significant difference in the degree of histodifferentiation of the tumors among the four groups. The animals in the two groups that received cortisone had significantly more tumors that were invasive than did the animals that did not receive cortisone. Cervical lymph node metastasis occurred in 21% to 38% of the animals but was not significantly different in the four groups. Distant metastases to the lungs or the liver were not found. Incisional biopsy of the tumors stimulated local growth of the cheek pouch tumors, and systemic cortisone administration produced more invasive cheek pouch tumors.

Incisional biopsy and seeding in hamster cheek pouch carcinoma.

Surgical blades were contaminated by doing incisional biopsies on DMBA-induced cheek pouch carcinomas in hamsters. Contaminated blades were then used to: (1) make cytologic smears; (2) make incisions in the mucosal surface of the lower lip; (3) make incisions in the ventral surface of the tongue; (4) make incisions in the untreated left cheek pouch; and (5) make incisions in the pre-shaved back. One group of animals was killed three hr post-surgery. The second group was killed seven wk post-surgery. Tissues were excised and processed for microscopic study. Malignant cells were found in many of the incision sites of animals from the first group. In the animals of the second group, several tumors growing from the transplanted tumor cells were observed in the incision sites of the lower lips and the tongue. None was observed in the incision sites of the left cheek pouch or the back. Cervical lymph node metastases were found in approximately 50% of the animals of the second group.

Laboratory grouping based on previous courses.

In the human physiology course at Loyola University School of Dentistry, students (N = 761, second-year classes from 1971 to 1976) were divided into three laboratory groups according to prior experience with physiology courses and laboratories. To determine the effects of this grouping on academic performance, data were obtained on grades in the physiology course, National Board scores in physiology (combined with biochemistry from 1973 to 1976), predental GPA, and predental science GPA. Comparisons were made between the three groups using t-tests, and correlation coefficients were obtained for physiology grade and National Board score, predental GPA, and predental science GPA. The results indicated that there were no significant differences among the three groups in performance in the physiology course or on the National Board Examination. Significant correlations were found between course performance and the other three variables. The overall impression of the faculty and the students was that the grouping program has been successful in improving laboratory performance and participation in the conference sessions.

Temporary auditory threshold shift following sophomore operative technique laboratory.

Twenty sophomore dental students were studied for the "temporary threshold shift" (TTS) due to exposure to the noise of high-speed dental headpieces during technique laboratory sessions. TTS was experienced at all four frequencies tested--8000, 6000, 4000, and 3000 hertz, but tended to be greater and of longer duration at the higher frequencies. Approximately one-fourth of the students still had some hearing loss 15 minutes after leaving the laboratory.