Asunto(s)
Anuros/embriología , Embrión no Mamífero/citología , Animales , División Celular , MorfogénesisRESUMEN
Influence of constant magnetic field (CMF-60 mT induction) on catecholamine content of the serous coats mast cell system was studied in 60 outbred albino rats by luminescent histochemical method. CMF exposition made twenty minutes daily during 70 days. Three days long one leaded to reduction of catecholamine content level in mast cell system. At the same time, CMF action from 6 to 10 days long caused increase of catecholamine level as compared to normal state in the structures studied. The data obtained were considered as a protective reaction of the organism, aimed to maintain the general homeostage balance.
Asunto(s)
Catecolaminas/análisis , Magnetismo , Mastocitos/química , Animales , Femenino , Masculino , Mastocitos/citología , Microscopía Fluorescente , Ratas , Espectrometría de Fluorescencia , Factores de TiempoRESUMEN
It was shown that the permanent magnetic fields at 35-40 mT1 inhibit the activity of biogenic monoamines in developing amphibian embryos. The effect was quantitatively similar to that of PMF at 40 mT1 (3-hour exposure during 6 days). The most significant fluctuations in their activity were observed at the stages of blastoderm formation and gastrulation onset.
Asunto(s)
Anfibios/embriología , Monoaminas Biogénicas/metabolismo , Magnetismo , Anfibios/metabolismo , Animales , Histocitoquímica , Rana ridibunda , Federación de Rusia , Coloración y Etiquetado/métodos , Factores de TiempoRESUMEN
By means of luminescent histochemistry effect of a constant magnetic field with induction 60 mTl (exposition for 2, 6, 36 h and 7 days) has been studied in order to reveal contents of catecholamines in mesenteric mast cells and in the intestinal mesentery cells in 50 white Wistar rats. In 2-6 h specific luminescence of the mast cells increases, however, at prolongation of the exposure up to 30 h metabolism of catecholamines in the mast cells is inhibited noticeably++ (luminescence disappears). In 8 days amount of the mast cells and specific luminescence of catecholamines decrease. The essential shifts revealed in the system of the mast cells of the mammalian should be taken into consideration in the magnetic-therapeutic practice. Close spatial relations between the mast cells and the mesenteric adrenergic terminals have been elucidated, demonstrating their morphofunctional interconnection.
Asunto(s)
Líquido Ascítico/citología , Catecolaminas/metabolismo , Campos Electromagnéticos , Mastocitos/efectos de la radiación , Mesenterio/citología , Animales , Catecolaminas/efectos de la radiación , Recuento de Células/efectos de la radiación , Masculino , Mastocitos/citología , Mastocitos/metabolismo , Ratones , Ratones Endogámicos , Ratas , Ratas EndogámicasRESUMEN
A sharp intensification of catecholamines synthesis in mast cells regularly followed noradrenaline and reserpine injection. The method of autoradiography makes it possible to analyse quantitatively the intensification of catecholamines synthesis in the mast cells.
Asunto(s)
Dihidroxifenilalanina/metabolismo , Mastocitos/metabolismo , Norepinefrina/farmacología , Reserpina/farmacología , Animales , Autorradiografía , Catecolaminas/biosíntesis , Femenino , Intestino Delgado/metabolismo , Masculino , Mastocitos/efectos de los fármacos , Mesenterio/metabolismo , Ratones , Ratones Endogámicos , Miocardio/metabolismo , Ratas , Ratas Endogámicas , Piel/metabolismo , Timo/metabolismo , Lengua/metabolismoRESUMEN
Histochemical fluorescent technique employed in the in vitro experiment detected stimulating effect of PND on the levels of catecholamines in peritoneal mast cells.
Asunto(s)
Catecolaminas/metabolismo , Mastocitos/efectos de los fármacos , Naftalenos/farmacología , Animales , Células Cultivadas , Diaminas/farmacología , Histocitoquímica , Mediciones Luminiscentes , Mastocitos/metabolismo , Ratas , Ratas EndogámicasRESUMEN
Reproduction of mast cells has been investigated by means of H3-thymidine radioautography in 26 mature mice of CC-57 White line and in 40 new-born nonlinear albino rats under normal conditions and experimental cold effect. It is established that differentiated mast cells incorporate H3-thymidine and can enter the phase of DNA synthesis of a proliferative cycle.