RESUMEN
OBJECTIVE: The aim of the present study was to reveal the possible effect of sulforaphane on oxidative stress and inflammation in rats liver with toxic hepatitis induced by acetaminophene. BACKGROUND: Sulforaphane is a compound with high antioxidant properties. Acetaminophen, which is a para-aminophenol derivative, can lead to fatal hepatic necrosis with direct hepatotoxic effects at high doses. METHODS: Thirty six male Sprague-Dawley rats were randomly divided into four groups. Control group (n = 9) was fed with standard rat chow and water for 3 days. Group APAP (n = 9) received a single dose acetaminophen 1 g/kg by oral gavage in addition to standard chow and water. Group SFN (n = 9) received sulforaphane 500 µg/kg by oral gavage in addition to standard chow and water for 3 days. Group APAP+SFN (n = 9) received sulforaphane 500 µg/kg and a single dose acetaminophen 1 g/kg by oral gavage in addition to standard chow and water. Acetaminophen was administered three hours after SFN administration. RESULTS: Neopterin, MDA, AST, ALT and CRP levels of group APAP were significantly increased compared to control group. GSH level of group APAP was significantly lower than in the control group. CONCLUSION: Sulforaphane is a protective agent against acetaminophen-induced liver damage and it can be added in the treatment protocol (Tab. 1, Fig. 5, Ref. 51).
Asunto(s)
Acetaminofén/toxicidad , Analgésicos no Narcóticos/toxicidad , Antioxidantes/farmacología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Isotiocianatos/farmacología , Hígado/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Alanina Transaminasa/efectos de los fármacos , Alanina Transaminasa/metabolismo , Animales , Aspartato Aminotransferasas/efectos de los fármacos , Aspartato Aminotransferasas/metabolismo , Proteína C-Reactiva/efectos de los fármacos , Proteína C-Reactiva/metabolismo , Inflamación , Hígado/metabolismo , Masculino , Malondialdehído/metabolismo , Neopterin/metabolismo , Extractos Vegetales/farmacología , Sustancias Protectoras/farmacología , Ratas , Ratas Sprague-Dawley , SulfóxidosRESUMEN
BACKGROUND: Ozone therapy is employed as a therapeutic agent in various diseases. Since ozone itself is a radical, using a small dosage of it is known to create an oxidative preconditioning in the body and trigger a strong antioxidant response against that. Coenzyme Q(10), as a strong antioxidant agent, is delivered as a supportive factor in many diseases involving oxidative stress. AIM: The aim of the study was to evaluate the effects of the combination treatment over oxidative stress in healthy individuals. METHODS: In the current study, 11 healthy volunteers were administered a combination of ozone therapy and Q(10) for 1 month. Blood samples were collected first right after the initial ozone therapy and then 1 month after the ozone therapy + coenzyme Q(10) treatment. We measured erythrocyte superoxide dismutase and catalase enzyme activities along with serum malondialdehyde levels. RESULTS: Compared with the pretreatment values, an increase was determined in the erythrocyte superoxide dismutase and catalase enzyme activities. However, malondialdehyde, an indicator of oxidative membrane damage, showed a reduction after the combination treatment. CONCLUSION: The results of this study reveal the beneficial effects of ozone therapy + coenzyme Q(10) combination in prevention of oxidative damage.
