Estudio piloto para determinar la asociación entre la microbiota intestinal y la fractura de cadera por fragilidad / Pilot study to determine the association between gut microbiota and fragility hip fracture

Introducción: Las fracturas de cadera son la causa más frecuente de ingreso hospitalario en los servicios de ortopedia de Europa y suponen un importante problema sanitario. Por ello, es de gran interés identificar factores de riesgo adicionales que nos ayuden a comprender mejor la fisiopatología de estas fracturas y a mejorar nuestra capacidad preventiva. Existen datos suficientes para apoyar la teoría de la modulación de la masa ósea por la microbiota intestinal (osteomicrobiología); sin embargo, faltan estudios clínicos en humanos que relacionen directamente la microbiota con el riesgo de fractura de cadera. Material y métodos: Estudio observacional, analítico, de casos y controles. La muestra consta de 50 pacientes y se distribuye de la siguiente manera: 25 pacientes ancianos con fractura de cadera por fragilidad y 25 controles sanos sin fractura. Se analizó la microbiota intestinal mediante extracción de ADN de muestras de heces y secuenciación del ADN ribosómico 16S tras la generación de bibliotecas de genes. Resultados: La diversidad alfa reveló una elevación de los estimadores para el nivel taxonómico de clase en el grupo de fracturas de cadera. Los órdenes Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales y Enterobacterales fueron los órdenes dominantes en ambos grupos. En los pacientes con fractura, se observó un aumento porcentual significativo del orden de Bacteroidales (p<0,001) y Peptostreptococcales-Tissierellales (p<0,005), así como una disminución de las del orden Lachnospirales (p<0,001) respecto a los controles. Conclusiones:Este estudio ha encontrado una asociación entre una microbiota específica en pacientes ancianos con fractura de cadera por fragilidad. Estos hallazgos abren la puerta a nuevas estrategias para prevenir las fracturas de cadera. Es posible que la modificación de la microbiota mediante probióticos se revele como un método eficaz para reducir el riesgo de fractura de cadera.(AU)

Introduction: Hip fractures are the most common cause of hospital admission to orthopaedic departments in Europe and they generate a major health problem. Therefore, it is of great interest to identify additional risk factors that will help us to better understand the pathophysiology of these fractures and improve our preventive capacity. There is sufficient data to support the theory of modulation of bone mass by gut microbiota (osteomicrobiology); however, there is a lack of human clinical studies directly linking microbiota to hip fracture risk. Material and methods: Observational, analytical, case–control study. The sample consisted of 50 patients and it was distributed as follows: 25 elderly patients with fragility hip fracture and 25 subjects without fracture. The intestinal microbiota was determined by DNA extraction from stool samples and 16S ribosomal DNA sequencing after generation of gene libraries. Results: Alpha diversity revealed an elevation of the estimators for the taxonomic class level in the hip fracture group. The orders Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales and Enterobacterales were the dominant orders in both groups. In patients with fracture, a significant percentage increase in the orders Bacteroidales (p<.001) and Peptostreptococcales-Tissierellales (p<.005) was observed, as well as a decrease in the orders Lachnospirales (p<.001) compared to controls. Conclusions: This study has found an association between a specific microbiota in elderly patients with fragility hip fracture. These findings open the door to new strategies to prevent hip fractures. Modification of the microbiota through probiotics may prove to be an effective method to reduce the risk of hip fracture.(AU)

[Translated article] Pilot study to determine the association between gut microbiota and fragility hip fracture / Estudio piloto para determinar la asociación entre la microbiota intestinal y la fractura de cadera por fragilidad

Introducción: Las fracturas de cadera son la causa más frecuente de ingreso hospitalario en los servicios de ortopedia de Europa y suponen un importante problema sanitario. Por ello, es de gran interés identificar factores de riesgo adicionales que nos ayuden a comprender mejor la fisiopatología de estas fracturas y a mejorar nuestra capacidad preventiva. Existen datos suficientes para apoyar la teoría de la modulación de la masa ósea por la microbiota intestinal (osteomicrobiología); sin embargo, faltan estudios clínicos en humanos que relacionen directamente la microbiota con el riesgo de fractura de cadera. Material y métodos: Estudio observacional, analítico, de casos y controles. La muestra consta de 50 pacientes y se distribuye de la siguiente manera: 25 pacientes ancianos con fractura de cadera por fragilidad y 25 controles sanos sin fractura. Se analizó la microbiota intestinal mediante extracción de ADN de muestras de heces y secuenciación del ADN ribosómico 16S tras la generación de bibliotecas de genes. Resultados: La diversidad alfa reveló una elevación de los estimadores para el nivel taxonómico de clase en el grupo de fracturas de cadera. Los órdenes Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales y Enterobacterales fueron los órdenes dominantes en ambos grupos. En los pacientes con fractura, se observó un aumento porcentual significativo del orden de Bacteroidales (p<0,001) y Peptostreptococcales-Tissierellales (p<0,005), así como una disminución de las del orden Lachnospirales (p<0,001) respecto a los controles. Conclusiones:Este estudio ha encontrado una asociación entre una microbiota específica en pacientes ancianos con fractura de cadera por fragilidad. Estos hallazgos abren la puerta a nuevas estrategias para prevenir las fracturas de cadera. Es posible que la modificación de la microbiota mediante probióticos se revele como un método eficaz para reducir el riesgo de fractura de cadera.(AU)

Introduction: Hip fractures are the most common cause of hospital admission to orthopaedic departments in Europe and they generate a major health problem. Therefore, it is of great interest to identify additional risk factors that will help us to better understand the pathophysiology of these fractures and improve our preventive capacity. There is sufficient data to support the theory of modulation of bone mass by gut microbiota (osteomicrobiology); however, there is a lack of human clinical studies directly linking microbiota to hip fracture risk. Material and methods: Observational, analytical, case–control study. The sample consisted of 50 patients and it was distributed as follows: 25 elderly patients with fragility hip fracture and 25 subjects without fracture. The intestinal microbiota was determined by DNA extraction from stool samples and 16S ribosomal DNA sequencing after generation of gene libraries. Results: Alpha diversity revealed an elevation of the estimators for the taxonomic class level in the hip fracture group. The orders Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales and Enterobacterales were the dominant orders in both groups. In patients with fracture, a significant percentage increase in the orders Bacteroidales (p<.001) and Peptostreptococcales-Tissierellales (p<.005) was observed, as well as a decrease in the orders Lachnospirales (p<.001) compared to controls. Conclusions: This study has found an association between a specific microbiota in elderly patients with fragility hip fracture. These findings open the door to new strategies to prevent hip fractures. Modification of the microbiota through probiotics may prove to be an effective method to reduce the risk of hip fracture.(AU)

[Translated article] Pilot study to determine the association between gut microbiota and fragility hip fracture.

INTRODUCTION: Hip fractures are the most common cause of hospital admission to orthopaedic departments in Europe and they generate a major health problem. Therefore, it is of great interest to identify additional risk factors that will help us to better understand the pathophysiology of these fractures and improve our preventive capacity. There is sufficient data to support the theory of modulation of bone mass by gut microbiota (osteomicrobiology); however, there is a lack of human clinical studies directly linking microbiota to hip fracture risk. MATERIAL AND METHODS: Observational, analytical, case-control study. The sample consisted of 50 patients and it was distributed as follows: 25 elderly patients with fragility hip fracture and 25 subjects without fracture. The intestinal microbiota was determined by DNA extraction from stool samples and 16S ribosomal DNA sequencing after generation of gene libraries. RESULTS: Alpha diversity revealed an elevation of the estimators for the taxonomic class level in the hip fracture group. The orders Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales and Enterobacterales were the dominant orders in both groups. In patients with fracture, a significant percentage increase in the orders Bacteroidales (p<.001) and Peptostreptococcales-Tissierellales (p<.005) was observed, as well as a decrease in the orders Lachnospirales (p<.001) compared to controls. CONCLUSIONS: This study has found an association between a specific microbiota in elderly patients with fragility hip fracture. These findings open the door to new strategies to prevent hip fractures. Modification of the microbiota through probiotics may prove to be an effective method to reduce the risk of hip fracture.

Pilot study to determine the association between gut microbiota and fragility hip fracture. / Estudio piloto para determinar la asociación entre la microbiota intestinal y la fractura de cadera por fragilidad.

INTRODUCTION: Hip fractures are the most common cause of hospital admission to orthopaedic departments in Europe and they generate a major health problem. Therefore, it is of great interest to identify additional risk factors that will help us to better understand the pathophysiology of these fractures and improve our preventive capacity. There is sufficient data to support the theory of modulation of bone mass by gut microbiota (osteomicrobiology); however, there is a lack of human clinical studies directly linking microbiota to hip fracture risk. MATERIAL AND METHODS: Observational, analytical, case-control study. The sample consisted of 50 patients and it was distributed as follows: 25 elderly patients with fragility hip fracture and 25 subjects without fracture. The intestinal microbiota was determined by DNA extraction from stool samples and 16S ribosomal DNA sequencing after generation of gene libraries. RESULTS: Alpha diversity revealed an elevation of the estimators for the taxonomic class level in the hip fracture group. The orders Bacteroidales, Oscillospirales, Lachnospirales, Peptostreptococcales-Tissierellales and Enterobacterales were the dominant orders in both groups. In patients with fracture, a significant percentage increase in the orders Bacteroidales (p<.001) and Peptostreptococcales-Tissierellales (p<.005) was observed, as well as a decrease in the orders Lachnospirales (p<.001) compared to controls. CONCLUSIONS: This study has found an association between a specific microbiota in elderly patients with fragility hip fracture. These findings open the door to new strategies to prevent hip fractures. Modification of the microbiota through probiotics may prove to be an effective method to reduce the risk of hip fracture.

Aptitudes y conocimientos en medicina musculoesquelética en licenciados en medicina previos a su incorporación al sistema de residencia del Sistema Nacional de Salud / Skills and knowledge in musculoskeletal medicine in medical graduates prior to their incorporation into the residency system of the National Health System

Introducción: La enfermedad del sistema musculoesquelético es una causa frecuente de demanda asistencial. El conocimiento en medicina musculoesquelética (MME) debe ser esencial para gran parte de las especialidades. Desafortunadamente, gran parte de los médicos internos residentes (MIR) reconocen tener falta de confianza y de competencia en este campo. Material y métodos: Cincuenta MIR recién incorporados a su plaza (32 de ellos residentes de COT de la Comunidad Valenciana) completaron el test de competencia básica en MME de Freedman y Berstein. Además, realizaron un cuestionario sobre el grado de confianza a la hora de desempeñar 5 tareas habituales en la práctica clínica y sobre la percepción de la carga curricular de MME en su formación académica. Resultados: La puntuación media global obtenida en el test fue de 69,44% (SD 13,32%), mientras que la puntuación concreta para 5 preguntas que se consideraban «banderas rojas» fue de 14,34%(SD 2,58%). Ambas mostraron diferencias significativas entre los residentes de COT y otras especialidades.La mediana obtenida en la valoración del nivel de confianza en la exploración de heridas fue de 2 sobre 5 (IQR 2), con diferencias significativas (p=0,014) entre el grupo COT y el de otras especialidades. La percepción del tiempo dedicado en la facultad en materia de MME fue considerada como adecuada (mediana 3, IQR 1). El 64% modificaría el planteamiento de la parte práctica del currículo en MME. Conclusiones: La prueba global fue superada por el 50% de los residentes, lo cual pone de manifiesto que la enseñanza en MME es deficitaria durante el periodo universitario y formativo preMIR. Consideramos primordial la planificación de acciones formativas que se traduzca en un aumento de los conocimientos y aptitudes necesarias para su correcto manejo, ya que ello se traduciría en una mayor agilidad del sistema, una mejor asistencia sanitaria y un mejor cribado de derivaciones especializadas.(AU)

Introduction: Pathology of the musculoskeletal system is a frequent cause of healthcare requirements. Knowledge of musculoskeletal medicine (MSM) should be essential for most specialties. Unfortunately, many medical intern residents (MIRs) admit to a lack of confidence and competence in this field. Material and methods: 50 recently hired MIRs (32 of whom were COT residents from the Comunidad Valenciana) completed the Freedman and Berstein test of basic competency in MSM. In addition, they completed a questionnaire about their confidence in performing five common tasks in clinical practice and their perception of the curricular importance of medicine in their academic training. Results: The overall mean score obtained on the test was 69.44% (SD 13.32%), while the specific score for 5 “red flags” questions was 14.34% (SD 2.58%). Both of them showed significant differences between COT residents and other specialties.The median obtained in the evaluation of the level of confidence in wound examination was 2 above 5 (IQR 2), with significant differences (p=.014) between the COT group and other specialties. The perception of the time spent in the faculty on MSM was considered adequate (median 3, IQR 1). 64% of participants would modify the approach to the practical part of the curriculum in MME. Conclusions: The overall test was passed by 50% of the residents, which shows that the teaching of MME is deficient during the university and pre-MIR training period. We believe that it is important to plan training actions to increase the knowledge and skills necessary for its correct handling; this way, the system would be more efficient with better health care and a better screening of specialised derivations.(AU)

Skills and knowledge in musculoskeletal medicine in medical graduates prior to their incorporation into the residency system of the National Health System / Aptitudes y conocimientos en medicina musculoesquelética en licenciados en medicina previo a su incorporación al sistema de residencia del Sistema Nacional de Salud

Introduction: Pathology of the musculoskeletal system is a frequent cause of healthcare requirements. Knowledge of musculoskeletal medicine (MSM) should be essential for most specialties. Unfortunately, many medical intern residents (MIRs) admit to a lack of confidence and competence in this field. Material and methods: 50 recently hired MIRs (32 of whom were COT residents from the Comunidad Valenciana) completed the Freedman and Berstein test of basic competency in MSM. In addition, they completed a questionnaire about their confidence in performing five common tasks in clinical practice and their perception of the curricular importance of medicine in their academic training. Results: The overall mean score obtained on the test was 69.44% (SD 13.32%), while the specific score for 5 “red flags” questions was 14.34% (SD 2.58%). Both of them showed significant differences between COT residents and other specialties.The median obtained in the evaluation of the level of confidence in wound examination was 2 above 5 (IQR 2), with significant differences (p=.014) between the COT group and other specialties. The perception of the time spent in the faculty on MSM was considered adequate (median 3, IQR 1). 64% of participants would modify the approach to the practical part of the curriculum in MME. Conclusions: The overall test was passed by 50% of the residents, which shows that the teaching of MME is deficient during the university and pre-MIR training period. We believe that it is important to plan training actions to increase the knowledge and skills necessary for its correct handling; this way, the system would be more efficient with better health care and a better screening of specialised derivations.(AU)

Introducción: La enfermedad del sistema musculoesquelético es una causa frecuente de demanda asistencial. El conocimiento en medicina musculoesquelética (MME) debe ser esencial para gran parte de las especialidades. Desafortunadamente, gran parte de los médicos internos residentes (MIR) reconocen tener falta de confianza y de competencia en este campo. Material y métodos: Cincuenta MIR recién incorporados a su plaza (32 de ellos residentes de COT de la Comunidad Valenciana) completaron el test de competencia básica en MME de Freedman y Berstein. Además, realizaron un cuestionario sobre el grado de confianza a la hora de desempeñar 5 tareas habituales en la práctica clínica y sobre la percepción de la carga curricular de MME en su formación académica. Resultados: La puntuación media global obtenida en el test fue de 69,44% (SD 13,32%), mientras que la puntuación concreta para 5 preguntas que se consideraban «banderas rojas» fue de 14,34%(SD 2,58%). Ambas mostraron diferencias significativas entre los residentes de COT y otras especialidades.La mediana obtenida en la valoración del nivel de confianza en la exploración de heridas fue de 2 sobre 5 (IQR 2), con diferencias significativas (p=0,014) entre el grupo COT y el de otras especialidades. La percepción del tiempo dedicado en la facultad en materia de MME fue considerada como adecuada (mediana 3, IQR 1). El 64% modificaría el planteamiento de la parte práctica del currículo en MME. Conclusiones: La prueba global fue superada por el 50% de los residentes, lo cual pone de manifiesto que la enseñanza en MME es deficitaria durante el periodo universitario y formativo preMIR. Consideramos primordial la planificación de acciones formativas que se traduzca en un aumento de los conocimientos y aptitudes necesarias para su correcto manejo, ya que ello se traduciría en una mayor agilidad del sistema, una mejor asistencia sanitaria y un mejor cribado de derivaciones especializadas.(AU)

Effects of myofascial release in erector spinae myoelectric activity and lumbar spine kinematics in non-specific chronic low back pain: Randomized controlled trial.

BACKGROUND: Flexion-relaxation response of the lumbar erector spinae has been previously studied after different interventions such as exercise programs or spinal manipulation, in subjects with chronic low back pain. The objective of the study was to investigate the effects of an isolated myofascial release protocol on erector spinae myoelectric activity and lumbar spine kinematics in chronic low back pain. METHODS: Thirty-six participants, with nonspecific chronic low back pain, were randomized to myofascial release group (n = 18) receiving four sessions of myofascial treatment, each lasting 40 min, and to control group (n = 18) receiving a sham myofascial release. Electromyographic and kinematic variables as well as pain and disability questionnaires were analyzed. FINDINGS: There was a bilateral reduction of the flexion relaxation ratio in individuals receiving myofascial release and who did not show myoelectric silence at baseline (right difference M = 0.34, 95% CI [0.16, 0.33], p ≤ .05 and left difference M = 0.45, 95% CI [0.16, 0.73], p ≤ .05). There was also a significant reduction in pain in the myofascial release group (difference M = -9.1, 95% CI [-16.3, -1.8], p ≤ .05) and disability (difference M = -5.6, 95% CI [-9.1, -2.1], p ≤ .05), compared with control group. No significant differences between groups were found for the kinematic variables. INTERPRETATION: The myofascial release protocol contributed to the normalization of the flexion- relaxation response in individuals who did not show myoelectric silence before the intervention, and also showed a significant reduction in pain and disability compared with the sham group.

Effects of unstable shoes on trunk muscle activity in patients with chronic low back pain.

INTRODUCTION: Unstable shoes were developed as a walking device to strengthen the lower extremity muscles and reduce joint loading. Many studies have reported increased muscle activity throughout the gait cycle in most of the lower limb muscles in healthy adults using these shoes. However, no previous studies have explored the effects of wearing unstable shoes on trunk muscle activity in patients with chronic low back pain (CLBP). Therefore, the aim of the present study was to compare the activity of selected trunk muscles in patients with CLBP during a gait test while walking wearing unstable shoes or conventional flat shoes (control). METHODS: Thirty-five CLBP patients (51.1 ±â€¯12.4 y; 26 ±â€¯3.8 kg/m2; 9.3 ±â€¯5.2 Roland Morris Disability Questionnaire score) were recruited from the Orthopedic Surgery Service at the Hospital to participate in this cross-sectional study. All the participants underwent gait analysis by simultaneously collecting surface electromyography (EMG) data from erector spinae (ES), rectus abdominis (RA), obliquus internus (OI), and obliquus externus (OE) muscles, while walking on a treadmill with flat control shoes or experimental unstable shoes. RESULTS: The results showed significantly higher %EMG activity in the ES (mean difference: 1.8%; 95% CI: 1.3-2.2), RA (mean difference: 1.5%; 95% CI: 0.3-2.7), and OI (mean difference: 1.5%; 95% CI: 0.2-2.8) in the unstable versus the flat-shoe condition, with a large effect size for the ES (Cohen's d = 1.27). CONCLUSIONS: Based on these findings, the use of unstable shoes may be implicated in promoting spine stability, particularly in improving neuromuscular control of the trunk muscles in CLBP treatment.