Inhibition effect of epicatechin gallate on acid phosphatases from rainbow trout (Oncorhynchus mykiss) liver by multispectral and molecular docking.

Inhibition of acid phosphatase, which significantly contributes to inosine 5'-monophosphate (IMP) degradation, is crucial for preventing flavor deterioration of aquatic products during storage. In this study, the inhibitory effect of epicatechin gallate (ECG) on the activity of acid phosphatase isozymes (ACPI and ACPII) was analyzed using inhibition kinetics, fluorescence spectroscopy, isothermal titration calorimetry, and molecular simulation. ACPI and ACPII with molecular weights of 59.5 and 37.3 kDa, respectively, were purified from rainbow trout liver. ECG reversibly inhibited ACPI and ACPII activities via mixed-type inhibition, with half maximal inhibitory concentration (IC50) of 0.24 ± 0.01 mmol/L and 0.27 ± 0.03 mmol/L, respectively. Fluorescence spectra indicated that ECG statically quenched the intrinsic fluorescence of ACPI and ACPII. ECG could spontaneously bind to ACPI and ACPII through hydrogen bonding and van der Waals forces and exhibited a higher affinity for ACPI than for ACPII. In addition, molecular dynamic simulation revealed that ECG-ACPI and ECG-ACPII complexes were relatively stable during the entire simulation process. Our findings provide a theoretical basis for the use of ECG as an inhibitor of ACP to improve the flavor of aquatic products.

Excitability changes induced in the human auditory cortex by transcranial alternating current stimulation.

Transcranial alternating current stimulation (tACS) has been widely studied for its ability to regulate motor, perceptual, and cognitive functions. Given the unique frequency specificity of tACS, it is expected to directly target rhythmic activity in the typical electroencephalogram (EEG) range. After tACS stimulation, changes in stimulation-induced and evoked activities can be inspected. Detecting changes in auditory evoked activity after different frequencies of tACS stimulation will be helpful for further revealing the influence of tACS on the excitation/inhibition of γ activity in the auditory cortex. Using a randomized repeated measures design, this study assessed the effects of alpha(α)-tACS and gamma(γ)-tACS on the auditory steady-state response (ASSR) in 11 normal-hearing participants. Participants attended four sessions held at least one week apart, receiving tACS or sham treatment. The results indicated that α-tACS had an inhibitory effect on 40-Hz ASSR compared to both γ-tACS and sham tACS, which occurred 30 min after stimulation. Taken together, these findings contribute to the understanding of tACS-induced excitability changes in the human auditory cortex, helping reveal the neurophysiological changes after tACS.

Depression of auditory cortex excitability by transcranial alternating current stimulation.

Transcranial alternating current stimulation (tACS) is a type of noninvasive brain stimulation technique that has been shown to modulate motor, cognitive and memory function. Direct electrophysiological evidence of an interaction between tACS and the auditory cortex excitability has rarely been reported. Different stimulation parameters and areas of tACS may have different influence on the regulatory results. In this study, 11-Hz tACS was applied to the auditory cortex of 12 subjects with normal hearing in order to explore its effects on the auditory steady-state response (ASSR). The results indicate that tACS has an inhibitory effect on 40-Hz ASSR. In addition, EEG source analysis shows that 11-Hz tACS may enhance the activity of the middle temporal gyrus under both sham and real conditions, while the estimated source activity of the posterior cingulate gyrus may be reduced under real condition. The results reveal that tACS applied to the temporal lobe of humans will make the 40-Hz ASSR a tendency to decrease, and help improve the understanding of modulation of tACS-induced auditory cortex excitability changes in humans.

Effects of Transcranial Electrical Stimulation on Human Auditory Processing and Behavior-A Review.

Transcranial electrical stimulation (tES) can adjust the membrane potential by applying a weak current on the scalp to change the related nerve activity. In recent years, tES has proven its value in studying the neural processes involved in human behavior. The study of central auditory processes focuses on the analysis of behavioral phenomena, including sound localization, auditory pattern recognition, and auditory discrimination. To our knowledge, studies on the application of tES in the field of hearing and the electrophysiological effects are limited. Therefore, we reviewed the neuromodulatory effect of tES on auditory processing, behavior, and cognitive function and have summarized the physiological effects of tES on the auditory cortex.