Pathogenicity of a variant goose parvovirus, from short beak and dwarfism syndrome of Pekin ducks, in goose embryos and goslings.

The pathogenicity of a variant goose parvovirus (GPV), isolated from short beak and dwarfism syndrome of Pekin ducks (strain Cherry Valley), was investigated in embryonating goose eggs and goslings. The virus was easily grown in GPV antibody-free goose embryos and caused high mortality and severe lesions of goose embryos, indicating that the variant GPV has good adaptation and high pathogenicity to embryonated goose eggs similar to the classical GPV. Like the third egg-passage virus (strain H) of a classical GPV, the third egg-passage virus (strain JS1) of the variant GPV caused Derzsy's disease in 2-day-old goslings with high mortality. The findings suggest that the variant GPV strain, which had specifically adapted to Pekin ducks, still retained high pathogenicity for its original host. The mortality (73.3-80%) caused by the first and third egg-passages of the variant GPV was somewhat lower than that (93.3%) caused by the third passage virus of the classical GPV, reflecting the higher pathogenicity of the classical GPV for its original host. These findings are likely to reinforce the importance of surveillance for parvoviruses in different waterfowl species and stimulate further study to elucidate the impact of mutations in the GPV genome on its pathogenicity to goslings and ducks.

Genetic characterization of a novel calicivirus from a goose.

A novel calicivirus (strain H146) was detected in a goose and sequenced. The H146 genome consisted of two open reading frames (ORFs) with an 8-nucleotide (nt) overlap between the two ORFs, similar to what has been found in the bat sapovirus TLC58. The virus was most closely related to nacoviruses when comparing the complete genome sequence (49% identity), non-structural region (NS; 31-34% amino acid [aa] sequence identity), and major structural VP1 region (28-30% aa identity), whereas both goose calicivirus N and feline calicivirus were the closest relatives of H146 in the VP2 region (20% aa sequence identity). The levels of divergence between H146 and its closest relatives in different genomic regions are comparable to those between some members of different genera. Phylogenetic analysis based on the NS and VP1 amino acid sequences clearly demonstrated that H146 formed a separate clade. Thus, calicivirus H146 was identified as a founding member of a novel genus for which we propose the name "Sanovirus".