Characterization of Shallow Ground in Railway Embankments Using Surface Waves Measured by Dark Fiber Optics Sensors: A Case Study.

For the maintenance of railways on soft soils, accurate knowledge of the subsoil conditions is essential. Soft soils at shallow depths have high variability; thus, high spatial resolution is required. Spare telecommunication fiber-optic cables, known as dark fiber, can be used as an array of sensors to measure waves generated by running trains, which offers a unique opportunity to characterize shallow soils at high spatial resolution. We used dark fiber to measure seismic waves generated by running trains and implemented a seismic interferometry technique to retrieve surface waves. We evaluated the reliability of selected parts of the recorded signals split as bow waves (the train approaching the fiber), train waves (the train passing alongside the fiber), and tail waves (the train leaving the fiber) to retrieve broad-band surface waves. The analysis was performed in two distinctive zones. Zone I consists of a thick-soft (2.0-6.0 m thickness) layer, and Zone II consists of a thin-soft (less than 2.0 m thickness) layer, both overlaying a "stiffer" sand layer. At Zone I, train waves yielded the best results in revealing the thick-soft layer. At Zone II, the bow waves yielded clear high-frequency energy, revealing the overall soil structure but without identifying the shallow thin-soft layer.

Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi.

Global food security depends on cereal crops with durable disease resistance. Most cereals are colonized by rust fungi, which are pathogens of major significance for global agriculture1. Cereal rusts display a high degree of host specificity and one rust species or forma specialis generally colonizes only one cereal host2. Exploiting the non-host status and transferring non-host resistance genes between cereal crop species has been proposed as a strategy for durable rust resistance breeding. The molecular determinants that define the host status to rusts, however, are largely unknown. Here, we show that orthologous genes at the Rphq2 locus for quantitative leaf rust resistance from cultivated barley3 and Rph22 from wild bulbous barley4 affect the host status to leaf rusts. Both genes encode lectin receptor-like kinases. We transformed Rphq2 and Rph22 into an experimental barley line that has been bred for susceptibility to non-adapted leaf rusts, which allowed us to quantify resistance responses against various leaf rust species. Rphq2 conferred a much stronger resistance to the leaf rust of wild bulbous barley than to the leaf rust adapted to cultivated barley, while for Rph22 the reverse was observed. We hypothesize that adapted leaf rust species mitigate perception by cognate host receptors by lowering ligand recognition. Our results provide an example of orthologous genes that connect the quantitative host with non-host resistance to cereal rusts. Such genes provide a basis to exploit non-host resistance in molecular breeding.