[Gender-specific differences in relative effects of cardiovascular risk factors among rural population].

OBJECTIVE: To investigate the prevalence and association of cardiovascular disease and its risk factors in Fangshan District, Beijing. METHODS: A total of 7 577 rural residents aged over 40 years were surveyed to estimate the prevalence of cardiovascular disease and its risk factors by questionnaire survey, physical examination and biochemical measures. The prevalence odds ratio (POR) was calculated for the association of stroke / coronary heart disease (CHD) with its risk factors in multiple logistic regression models. RESULTS: Prevalence of CHD, stroke, diabetes, hypertension, dyslipidemia, overweight and obesity was presented as 6.5%, 4.1%, 10.7%, 39.5%, 56.7%, 44.0% and 13.6%, respectively. In the adjusted multiple logistic regression models, hypertension showed stronger association with stroke (POR=2.780, 95%CI: 2.146-3.602), while diabetes had a higher correlation with CHD (POR=2.671, 95%CI: 2.121-3.363). After stratification by gender, significant risk factors for stroke were overweight / obesity (POR=1.652, 95%CI: 1.122-2.433), body mass index (POR=1.048, 95%CI: 1.002- 1.096), systolic blood pressure (POR=1.017, 95%CI: 1.005-1.030) and serum glucose (POR=1.093, 95%CI: 1.048-1.140) in females, whereas dyslipidemia (POR=1.615, 95%CI: 1.124-2.319), total cholesterol (POR=3.932, 95%CI: 1.533-10.086) and high-density lipoprotein cholesterol (POR=0.072, 95%CI: 0.022-0.236) were associated with stroke in males. CONCLUSION: Highly prevalent cardiovascular risk factors aggravated the burden brought by stroke and CHD in the rural population, and the various risk factors for stroke and CHD exhibited heterogeneous contributions among the population with different gender.

[Strong association between the NINJ2 gene polymorphism and the susceptibility of stroke in Chinese Han population in Fangshan district].

OBJECTIVE: To investigate the association between the NINJ2 gene rs11833579 polymorphism and stroke in Han Chinese population. METHODS: This study was a population-based cross-sectional case-control study. Polymerase chain reaction-restriction fragment length polymorphism (RFLP) and sequencing were used for the detection of NINJ2 genotypes in 790 patients with stroke (679 ischemic stroke) which were Han Chinese population from Fangshan First Hospital and 811 controls which were healthy Han Chinese population without family history of stroke in Fangshan district rural area. RESULTS: In rs11833579 locus of the NINJ2 gene, the frequencies of GG genotype and allele G were higher in ischemic stroke patients than that in controls (P<0.001). The frequency of allele G of the NINJ2 gene was higher in cerebral hemorrhage patients than that in controls (P=0.005). Genotype had little effect on the glucose, total cholesterol and triglyceride. CONCLUSION: There is significant association between rs11833579 site polymorphism of the NINJ2 gene and risk for stroke in Han Chinese population from Fangshan district.

[An association study of abdominal obesity and polymorphisms of UCP2 and SREBP1c genes].

OBJECTIVE: To investigate the association of -866A/G polymorphism of uncoupling protein 2 (UCP2) gene, and 54G/C polymorphism of sterol regulatory element binding protein 1c (SREBP1c) gene with abdominal obesity in the population of type 2 diabetes mellitus families. METHODS: Eligible type 2 diabetes mellitus cases from newly diagnosed and previous hospitalized patients were choson, then their family members (siblings and parents) tracked. A set of questionnaires was administered to obtain information on demographic characteristics. Physical measurements were recorded. DNA was extracted from blood samples and genotyped using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP). Generalized estimating equation (GEE) was used to adjust for within-family correlation in analysis of relationships of factors. RESULTS: In the study population, the frequency of A allele of UCP2-866A/G polymorphism was 0.459, and of G allele 0.541; the frequency of G allele of SREBP1c 54G/C polymorphism was 0.822, and of C allele 0.178. Totally 762 participants were analyzed using GEE regression. It was shown that the odds ratio (OR) of the population with only 54G/C polymorphism of SREBP1c gene being the mutant type (GC/CC) was statistically significant while -866A/G polymorphism of UCP2 gene not being the mutant type (AG/GG) was not. The OR of the population with the opposite genotype status was 1.8 (P=0.042), and that with mutant types of both polymorphisms 3.2(P=0.001). CONCLUSION: In the population of type 2 diabetes mellitus families, only 54G/C polymorphism of SREBP1c gene being the mutant type (GC/CC) might be a moderate risk factor of abdominal obesity. When both the two polymorphisms studied are the mutant type, the risk of abdominal obesity may increase significantly.

[Genetic epidemiological study on discordant sib pairs of ischemic stroke in Beijing Fangshan District].

OBJECTIVE: To investigate the association among serum lipids, blood pressure, -514C/T polymorphism of hepatic lipase gene and subtypes of ischemic stroke in the discordant sib pairs. METHODS: Ischemic stroke cases were enrolled from a hospital-based stroke registry, the proband-initiated contact was used to recruit pedigrees, and participants' information and blood samples were collected through the community-based health care networks in rural areas of China. Statistical analysis was performed by using the Wilcoxon signed rank test to compare quantitative differences between sib pairs and the McNemar test to compare qualitative differences. Generalized estimating equation (GEE) was used to adjust for within-family correlation in analysis of discordant sib pairs. Family based association test (FBAT) was applied to associations between serum lipids, blood pressure and the -514T allele in hepatic lipase gene. RESULTS: Totally 107 discordant sib pairs from 71 ischemic stroke patients pedigrees were analyzed by univariate and multivariate methods. The blood pressure and serum lipids were associated with ischemic stroke (P<0.05) The lipid level was higher in large-artery atherosclerosis ischemic stroke than in the subtype of small-vessel occlusion, while the blood pressure was higher in the latter. The -514T allele in hepatic lipase gene was associated with low density lipoprotein-cholesterol (LDL-C) and diastolic blood pressure in additive model by FBAT analysis (Z=2.366, P<0.05), and it was also associated with diastolic blood pressure in additive model (Z=-2.277, P<0.05) and recessive model (Z=-2.244,P< 0.05), but not associated with ischemic stroke or its subtypes. CONCLUSION: In these discordant sib pairs, the abnormalities of blood pressure and serum lipids may increase risks of ischemic stroke, and different subtypes of ischemic stroke may differ from each other in etiology. The -514T allele in hepatic lipase gene may be associated with LDL-C and diastolic blood pressure.