The Impact of Disturbed Soil Structure on the Degradation of 2 Fungicides Under Constant and Variable Moisture.

Degradation of agrochemicals in soil is frequently faster under field conditions than in laboratory studies. Field studies are carried out on relatively undisturbed soil, whereas laboratory studies typically use sieved soil, which can have a significant impact on the physical and microbial nature of the soil and may contribute to differences in degradation between laboratory and field studies. A laboratory study was therefore conducted to determine the importance of soil structure and variable soil moisture on the degradation of 2 fungicides (azoxystrobin and paclobutrazol) that show significant differences between laboratory and field degradation rates in regulatory studies. Degradation rates were measured in undisturbed cores of a sandy clay loam soil (under constant or variable moisture contents) and in sieved soil. For azoxystrobin, degradation rates under all conditions were similar (median degradation time [DegT50] 34-37 d). However, for paclobutrazol, degradation was significantly faster in undisturbed cores (DegT50 255 d in sieved soil and 63 d in undisturbed cores). Varying the moisture content did not further enhance degradation of either fungicide. Further examination into the impact of soil structure on paclobutrazol degradation, comparing undisturbed and sieved/repacked cores, revealed that the impact of sieving could not be mitigated by repacking the soil to a realistic bulk density. Examination of fungal and bacterial community structure using automated ribosomal spacer analysis showed significant initial differences between sieved/repacked and intact soil cores, although such differences were reduced at the end of the study (70 d). The present study demonstrates that disruption of soil structure significantly impacts microbial community structure, and for some compounds this may explain the differences between laboratory and field degradation rates. Environ Toxicol Chem 2021;40:2715-2725. © 2021 SETAC.

The route and rate of thiamethoxam soil degradation in laboratory and outdoor incubated tests, and field studies following seed treatments or spray application.

BACKGROUND: The route and rate of degradation of thiamethoxam in the laboratory and field was investigated. The effect of dark incubation versus light/dark cycles, seed treatment versus spray, and watering-in for spray application was explored in side-by-side trials. RESULTS: Geometric mean DT50 values were 75.4 days in OECD307 studies, and 18.3 (spray) and 16.5 (seed treatment) days in the field. In laboratory soil core studies DT50 values were 24.9 to 43.5 days, with the lowest value from the light/dark incubated soil core. Mean clothianidin formation was 19.7% applied thiamethoxam [mol/mol] in OECD307 studies and 17.5 (spray) and 3.4% (seed) in field trials. CONCLUSION: Soil DT50 values decreased with increasingly realistic tests (laboratory OECD307 to soil cores to soil cores with a light/dark cycle to field trials). The majority of the differences were associated with the soil treatment in OECD307 studies which destroys soil structure and retards the degradation rate; and from the impact on soil pore water movement in light/dark conditions. Degradation rates in the field were comparable between spray application and seed treatments. Maximum clothianidin concentrations were four-fold lower for seed treatments than for spray application in field studies. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.