Impact of intestinal parasites on microbiota and cobalamin gene sequences: a pilot study.

BACKGROUND: Approximately 30% of children worldwide are infected with gastrointestinal parasites. Depending on the species, parasites can disrupt intestinal bacterial microbiota affecting essential vitamin biosynthesis. METHODS: Stool samples were collected from 37 asymptomatic children from a previous cross-sectional Argentinian study. A multi-parallel real-time quantitative PCR was implemented for Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, Strongyloides stercoralis, Trichuris trichiura, Cryptosporidium spp., Entamoeba histolytica and Giardia duodenalis. In addition, whole-genome sequencing analysis was conducted for bacterial microbiota on all samples and analyzed using Livermore Metagenomic Analysis Toolkit and DIAMOND software. Separate analyses were carried out for uninfected, Giardia-only, Giardia + helminth co-infections, and helminth-only groups. RESULTS: For Giardia-only infected children compared to uninfected children, DNA sequencing data showed a decrease in microbiota biodiversity that correlated with increasing Giardia burden and was statistically significant using Shannon's alpha diversity (Giardia-only > 1 fg/µl 2.346; non-infected group 3.253, P = 0.0317). An increase in diversity was observed for helminth-only infections with a decrease in diversity for Giardia + helminth co-infections (P = 0.00178). In Giardia-only infections, microbiome taxonomy changed from Firmicutes towards increasing proportions of Prevotella, with the degree of change related to the intensity of infection compared to uninfected (P = 0.0317). The abundance of Prevotella bacteria was decreased in the helminths-only group but increased for Giardia + helminth co-infections (P = 0.0262). Metagenomic analysis determined cobalamin synthesis was decreased in the Giardia > 1 fg/µl group compared to both the Giardia < 1 fg/µl and the uninfected group (P = 0.0369). Giardia + helminth group also had a decrease in cobalamin CbiM genes from helminth-only infections (P = 0.000754). CONCLUSION: The study results may provide evidence for an effect of parasitic infections enabling the permissive growth of anaerobic bacteria such as Prevotella, suggesting an altered capacity of vitamin B12 (cobalamin) biosynthesis and potential impact on growth and development in children .

Teaching Opioid Tapering Through Guided Instruction.

BACKGROUND AND OBJECTIVES: Given the current opioid epidemic, national practice guidelines and many state laws are shifting the treatment paradigm for chronic, noncancer pain to a judicious use of opioids. This has prompted a need to teach family medicine residents how to appropriately taper opioids. We created a multifaceted approach to integrate teaching of opioid tapering into a family medicine curriculum with an emphasis on guided instruction. We assessed the degree to which this curriculum affected guideline-concordant opioid prescribing within the family medicine practice. METHODS: A retrospective chart review of 707 patients on chronic opioid therapy (COT) for noncancer pain was conducted before and after the incorporation of a guided instruction experience to the residency curriculum. The primary outcomes included the number of patients on chronic opioids, the average morphine equivalent daily (MED) per patient, the percentage of patients on >50 MED or >90 MED, and the number of patients on concomitant benzodiazepines. RESULTS: Of the original 707 patients, 188 tapered off COT. Of those remaining on COT, the average MED did not change (53.4±76.9 vs 58.5+89.1, P=0.053). The percentage of patients on >50 MED and >90 MED decreased significantly (30.6% vs 25.0%, P=0.001; 19.4% vs 14.0%, P=0.027). The total number of patients on concomitant benzodiazepine decreased from 212 to 131. CONCLUSIONS: Providing opportunities for guided instruction with opioid tapering allowed for an increased concordance with national practice guidelines.

Essential factors demonstrating readiness of primary care practices for clinical pharmacy services.

PURPOSE: The characteristics of primary care practices that are necessary to establish and maintain ambulatory care clinical pharmacy services were identified. METHODS: A focus group of 15 ambulatory care pharmacists in Western North Carolina developed a survey of 26 practice readiness statements pertaining to the development of clinical pharmacy services in primary care. National ambulatory care pharmacy experts were then surveyed using a modified Delphi model for consensus building to determine which items were essential. Four rounds of surveys were completed. After each round, statements were accepted as consensus, modified, or removed from the survey based on responses. Statements were deemed to have reached consensus when 80% of respondents were in agreement. RESULTS: A total of 6 statements reached agreement after 4 rounds of survey: (1) full integration into the team, (2) access to the electronic health record (EHR), (3) a physician or administrative champion, (4) appropriate equipment provided by the clinic, (5) a private room to see patients, and (6) a practice that is open to team-based care. CONCLUSION: An expert panel of ambulatory care pharmacists identified 6 factors that should be considered prior to establishing ambulatory care services in primary care practices. Of these, foundational elements included full integration into the care team, presence of a physician or administrative champion, and a practice that is ready for team-based care. Operational elements included access to the practice's EHR, equipment provided by the practice, and private space to see patients.

Community pharmacist-led intervention to identify persons with diabetes not on statin therapy.

OBJECTIVES: To demonstrate the effectiveness of a community pharmacist-led intervention to identify persons with diabetes not receiving statin therapy. SETTING: Five pharmacy locations within 1 district of a chain community pharmacy. PRACTICE DESCRIPTION: Walgreens Pharmacy is a national retail chain community pharmacy with global affiliations. Walgreens Pharmacy locations use clinical pharmacy services to provide complete quality patient care. PRACTICE INNOVATION: A pharmacist intervention workflow model and screening algorithm for the Centers for Medicare and Medicaid Services (CMS) Statin Use in Persons with Diabetes quality measure were developed to identify measure-eligible patients in a community pharmacy setting. This new measure was implemented within the Electronic Quality Improvement Platform for Plans and Pharmacies (EQuIPP) dashboard. The study aimed to demonstrate that community pharmacist intervention is effective for helping to close therapy gaps; the focus of this study was identifying patients who have diabetes but are not on statin therapy. EVALUATION: The primary outcome included the percentage of patients flagged for intervention by the pharmacist, and the secondary outcome included the number of statin prescriptions received for the measure-eligible intervention population. RESULTS: At study initiation, EQuIPP reported a total of 103 patients were SUPD measure eligible at the intervention pharmacies (combined). After the 90-day study duration, 29.1% (n = 30) of SUPD measure-eligible patients were identified with the use of the workflow intervention. From those identified, 40% (n = 12) gave verbal consent for the pharmacist to intervene with their physician, and of those, 58.3% (n = 7) received statin prescriptions. Pharmacy A increased their EQuIPP-based performance measure by 3.3%, Pharmacy B 4.2%, Pharmacy D 2.4%, and Pharmacy E 3.5%, and Pharmacy C decreased by 1.0%. CONCLUSION: Implementation of a community pharmacist-led identification process successfully identified SUPD measure-eligible patients. Community pharmacist-led intervention resulted in an increase in the number of statin prescriptions written for measure-eligible patients.

Cognitive functioning following epilepsy surgery.

Temporal lobe resection is the most common surgery for intractable epilepsy because of its proven efficacy in seizure control. However, patients who may benefit from the procedure might be deterred from surgical evaluation due to concerns of postoperative cognitive decline. Recent reports on long-term follow-up indicate that, similar to findings within the year after surgery, cognition remains relatively stable in the years following right temporal resection. The verbal memory decline often observed 1 year after left temporal resection persists over time, yet is mitigated to some extent by good seizure outcome. Although memory decline observed on testing is not typically accompanied by functional decline, a small proportion of patients do experience reductions in occupational or academic status. Recent advances in functional imaging and refinements in preoperative mapping promise better prediction and protection of cognitive functioning. Additionally, results from studies comparing cognitive outcome among different surgical techniques suggest that more restricted resections benefit some patients, whereas more extended resections might be appropriate in a select group of well-defined patients. Preliminary reports on alternate treatments such as vagal nerve stimulation suggest no direct influence on cognition, although improvement in quality of life has been reported. The decision to pursue surgical treatment must balance the potential benefit of seizure control with the potential impact and probability of cognitive decline.