RESUMEN
To evaluate toxicity of silver nanoparticles synthesized by using the reverse micelle formation method, the effects of nanoparticles on lipid peroxidation and morphological changes of cell membranes in human lymphocytes were studied. It was found that under the influence of nanoparticles a reduction in cell viability and formation of excessive levels of reactive oxygen species were observed. Silver nanoparticles at different concentrations activate the processes of lipid peroxidation and, as a consequence, led to morphological changes in human lymphocytes.
Asunto(s)
Peroxidación de Lípido , Linfocitos/citología , Linfocitos/metabolismo , Nanopartículas del Metal/química , Especies Reactivas de Oxígeno/metabolismo , Plata/química , HumanosRESUMEN
The effect of NO donors (sodium nitroprusside, S-nitrosoglutathione, dinitrosyl-iron complexes) on the functional and mechanical properties of human platelets and red blood cells has been investigated. It has been established by atomic force microscopy that NO donor-induced platelet disaggregation is accompanied by changes in the elastic properties of cells. It has been shown that, in the presence of NO donors, the detergent-induced hemolysis of red blood cells is delayed, and the elasticity modulus of these cells decreases. The results obtained indicate that NO donors regulate the structural and functional properties of platelets and red blood cells.