RESUMEN
The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.
Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Proteínas/genética , Proteínas/metabolismo , Células de Sertoli/fisiología , Testículo/metabolismo , Secuencia de Aminoácidos , Animales , Repetición de Anquirina , Células Cultivadas , Clonación Molecular , Técnicas para Inmunoenzimas , Masculino , Ratones , Datos de Secuencia Molecular , Proteínas Nucleares/inmunología , Proteínas/inmunología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Conejos , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Células de Sertoli/citología , Maduración Sexual , Testículo/citologíaRESUMEN
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RESUMEN
In the title 3:1 adduct, 3C(7)H(6)O(3)·C(5)H(5)N(5), an intra-molecular O-Hâ¯O hydrogen bond occurs in each of the three 2-hydroxy-benzoic acid mol-ecules. In the crystal, the components are linked by N-Hâ¯O and O-Hâ¯N hydrogen bonds.
RESUMEN
In the title mol-ecule, C(23)H(19)N(3)O(3), the dihedral angles between the mean plane of the benzotriazole ring system and the benzene and phenyl rings are 9.67â (9) and 86.08â (10)°, respectively. The dihedral angle between the benzene and phenyl rings is 85.89â (11)°. In the crystal structure, weak inter-molecular C-Hâ¯O hydrogen bonds link mol-ecules into chains along [010].
RESUMEN
In the title compound, [Cd(H(2)O)(6)](C(14)H(10)O(6)N(2)S(2))·2H(2)O, the complete cation and anion are each generated by crystallographic inversion symmetry. In the crystal structure, the components form a three-dimensional network by way of O-Hâ¯O and O-Hâ¯N hydrogen bonds.
RESUMEN
In the title compound, [Zn(C(7)H(6)NO(2))(2)(H(2)O)(2)], the Zn atom (site symmetry ) adopts a distorted trans-ZnN(2)O(4) octa-hedral coordination arising from two N,O-bidentate 5-methyl-pyridine-2-carboxyl-ate ligands and two water mol-ecules. In the crystal structure, mol-ecules form a layered network linked by O-Hâ¯O hydrogen bonds.
RESUMEN
The title compound, C(10)H(11)N(3)O(3), was prepared by the reaction of acetohydrazide and 1-(4-nitro-phen-yl)ethanone. The asymmetric unit contains two crystallographically independent mol-ecules. Inversion-related mol-ecules form dimers, in which two N-Hâ¯O hydrogen bonds generate an inter-molecular R(2) (2)(8) ring.