RESUMEN
OBJECTIVE: Different effects of microRNA-495 (miR-495) on human cancers have been exhibited in recent years. However, the specific function of miR-495 remains uncertain in non-small-cell lung cancer (NSCLC). Thus, we aim to explore the role of miR-495 in NSCLC. PATIENTS AND METHODS: The expressions of miR-495 and transcription factor 4 (TCF4) were detected through quantitative Real-time polymerase chain reaction (qRT-PCR) assay. Western blot was used to measure the protein expression of relative genes. The relationship between miR-495 and TCF4 was testified by the dual-luciferase reporter gene assay. The function of miR-495 was investigated through cell counting kit-8 (CCK-8) assay and transwell assay. RESULTS: MiR-495 was downregulated in NSCLC tissues. Overexpression of miR-495 inhibited the migration, invasion and proliferation of NSCLC cells. Further, TCF4 was a direct target gene of miR-495. TCF4 was highly expressed in NSCLC tissues. In addition, miR-495 inhibited the progression of NSCLC through targeting TCF4. Furthermore, miR-495 inhibited EMT and Wnt/ß-catenin pathway in NSCLC. CONCLUSIONS: MiR-495 inhibited the progression of NSCLC by targeting TCF4 and inactivating Wnt/ß-catenin pathway.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/genética , Neoplasias Pulmonares/genética , MicroARNs/genética , Factor de Transcripción 4/metabolismo , Línea Celular Tumoral , Movimiento Celular/genética , Proliferación Celular/genética , Progresión de la Enfermedad , Regulación hacia Abajo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Vía de Señalización Wnt/genética , beta Catenina/metabolismoRESUMEN
APC is a tumor suppressor gene that is involved in the processes of cell migration and adhesion, transcriptional activation, and apoptosis. The goal of this study was to evaluate the contribution of the APC rs383830 polymorphism to coronary heart disease (CHD) in Han Chinese. A total of 783 patients with CHD and 737 controls were tested in the current association study. Although our study did not identify an association between the APC rs383830 polymorphism and CHD, a breakdown analysis by gender indicated there was a significant contribution of the rs383830 T allele to the risk of CHD in males (P = 0.046, odds ratio = 1.267, 95% confidence interval = 1.004-1.598). In conclusion, our study suggested a male-specific association of the APC rs383830 polymorphism with CHD.
Asunto(s)
Proteína de la Poliposis Adenomatosa del Colon/genética , Alelos , Enfermedad Coronaria/genética , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple , Anciano , Pueblo Asiatico , Estudios de Casos y Controles , Enfermedad Coronaria/etnología , Enfermedad Coronaria/patología , Femenino , Expresión Génica , Frecuencia de los Genes , Estudio de Asociación del Genoma Completo , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Factores de Riesgo , Factores SexualesRESUMEN
Enzyme-linked immunosorbent assay (ELISA), Dot-ELISA and Dot-immunogold silver staining (Dot-IGSS) were simultaneously used to detect the specific IgG against Toxoplasma gondii in 65 patients infected with the protozoa. The positive rates were 86.51%, 92.51% and 98.64%, respectively. When ELISA and Dot-ELISA results were put together, the positive rate increased to 95.38%. When Dot-IGSS results were combined with those of ELISA or Dot-ELISA, the positive rate was raised to 100%. The difference in positive rate between ELISA and Dot-IGSS was significant (x2 = 6.93, p < 0.01), but no statistically significant differences were found between ELISA and Dot-ELISA or between Dot-ELISA and Dot-IGSS. Paired comparison of the reacting intensities of the sera in the 3 assays showed the correlations were highly significant (p < 0.001), with r = 0.608 between Dot-IGSS and Dot-ELISA, r = 0.8194 between Dot-IGSS and ELISA and r = 0.517 between Dot-ELISA and ELISA. Hence combination of different serological assays may increase their sensitivity and specificity for detecting the anti-Toxoplasma antibodies.
Asunto(s)
Anticuerpos Antiprotozoarios/aislamiento & purificación , Inmunoensayo/métodos , Inmunoglobulina G/análisis , Toxoplasma/inmunología , Toxoplasmosis/diagnóstico , Animales , Estudios de Casos y Controles , Ensayo de Inmunoadsorción Enzimática/métodos , Femenino , Humanos , Embarazo , Sensibilidad y EspecificidadRESUMEN
Dot-immunogold-silver staining (Dot-IGSS) and Dot-ELISA were used, with Taenia solium cyst fluid antigen, to detect specific antibodies in the sera of patients with cysticercosis. All 40 confirmed cases of cysticercosis were found to be positive when tested by 2 methods. The average titer of the sera detected by Dot-IGSS was 1:27,470, which was significantly higher than that detected by Dot-ELISA. The false positive rates were 2% and 4% in 50 healthy controls for Dot-IGSS and Dot-ELISA, respectively, with a low titer of 1:20.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Cisticercosis/diagnóstico , Immunoblotting , Tinción con Nitrato de Plata , Especificidad de Anticuerpos , Ensayo de Inmunoadsorción Enzimática , Femenino , Oro Coloide , Humanos , Inmunoglobulina G , Masculino , Compuestos OrganometálicosRESUMEN
All 35 confirmed clonorchiasis cases showed a positive reaction in dot-immunogold-silver staining (Dot-IGSS) with a mean serum titre of 1:1656, while none of the sera from 35 normal individuals reacted. A seroepidemiological survey of middle-school students revealed a positive rate of 17.0% (142/836), and 76.1% (105/138) of the serologically positive students were egg positive in stool examination. The egg positive rates in those with antibody at levels of 1+ to 4+ were 57.1% (28/49), 75.7% (28/37), 94.7% (36/38) and 92.9% (13/14) respectively. It is believed that Dot-IGSS can be used for the clinical diagnosis and epidemiological survey of clonorchiasis.
Asunto(s)
Clonorquiasis/diagnóstico , Clonorquiasis/epidemiología , China/epidemiología , Humanos , Inmunohistoquímica/métodos , Recuento de Huevos de Parásitos , Estudios Seroepidemiológicos , Pruebas Serológicas/métodosRESUMEN
Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Filariasis Linfática/diagnóstico , Ensayo de Inmunoadsorción Enzimática , Immunoblotting , Inmunohistoquímica , Wuchereria bancrofti/inmunología , Animales , Filariasis Linfática/inmunología , Humanos , Microfilarias/inmunología , Valor Predictivo de las PruebasRESUMEN
We report the use of immunogold-silver staining (IGSS), dot-ELISA and dot-IGSS methods in the study of clonorchiasis in China. These methods were employed to detect the antibody in sera from 40 clonorchiasis patients. The positive rates were 100%, 90.0% and 95.0%, respectively. When the three methods were used to examine 40 normal sera, the negative rates were 100%, 97.5% and 97.5%, respectively. These results suggest that IGSS, dot-ELISA and dot-IGSS are highly specific and sensitive in detecting anti-Clonorchis antibody in patients.
