Lactobacillus delbrueckii CIDCA 133 fermented milk modulates inflammation and gut microbiota to alleviate acute colitis.

Lactobacillus delbrueckii subsp. lactis CIDCA 133 is a health-promoting bacterium that can alleviate gut inflammation and improve the epithelial barrier in a mouse model of mucositis. Despite these beneficial effects, the protective potential of this strain in other inflammation models, such as inflammatory bowel disease, remains unexplored. Herein, we examined for the first time the efficacy of Lactobacillus delbrueckii CIDCA 133 incorporated into a fermented milk formulation in the recovery of inflammation, epithelial damage, and restoration of gut microbiota in mice with dextran sulfate sodium-induced colitis. Oral administration of Lactobacillus delbrueckii CIDCA 133 fermented milk relieved colitis by decreasing levels of inflammatory factors (myeloperoxidase, N-acetyl-ß-D-glucosaminidase, toll-like receptor 2, nuclear factor-κB, interleukins 10 and 6, and tumor necrosis factor), secretory immunoglobulin A levels, and intestinal paracellular permeability. This immunobiotic also modulated the expression of tight junction proteins (zonulin and occludin) and the activation of short-chain fatty acids-related receptors (G-protein coupled receptors 43 and 109A). Colonic protection was effectively associated with acetate production and restoration of gut microbiota composition. Treatment with Lactobacillus delbrueckii CIDCA 133 fermented milk increased the abundance of Firmicutes members (Lactobacillus genus) while decreasing the abundance of Proteobacteria (Helicobacter genus) and Bacteroidetes members (Bacteroides genus). These promising outcomes influenced the mice's mucosal healing, colon length, body weight, and disease activity index, demonstrating that this immunobiotic could be explored as an alternative approach for managing inflammatory bowel disease.

Recombinant probiotic Lactococcus lactis delivering P62 mitigates moderate colitis in mice.

Introduction and objective: p62 is a human multifunctional adaptor protein involved in key cellular processes such as tissue homeostasis, inflammation, and cancer. It acts as a negative regulator of inflammasome complexes. It may thus be considered a good candidate for therapeutic use in inflammatory bowel diseases (IBD), such as colitis. Probiotics, including recombinant probiotic strains producing or delivering therapeutic biomolecules to the host mucosal surfaces, could help prevent and mitigate chronic intestinal inflammation. The objective of the present study was to combine the intrinsic immunomodulatory properties of the probiotic Lactococcus lactis NCDO2118 with its ability to deliver health-promoting molecules to enhance its protective and preventive effects in the context of ulcerative colitis (UC). Material and methods: This study was realized in vivo in which mice were supplemented with the recombinant strain. The intestinal barrier function was analyzed by monitoring permeability, secretory IgA total levels, mucin expression, and tight junction genes. Its integrity was evaluated by histological analyses. Regarding inflammation, colonic cytokine levels, myeloperoxidase (MPO), and expression of key genes were monitored. The intestinal microbiota composition was investigated using 16S rRNA Gene Sequencing. Results and discussion: No protective effect of L. lactis NCDO2118 pExu:p62 was observed regarding mice clinical parameters compared to the L. lactis NCDO2118 pExu: empty. However, the recombinant strain, expressing p62, increased the goblet cell counts, upregulated Muc2 gene expression in the colon, and downregulated pro-inflammatory cytokines Tnf and Ifng when compared to L. lactis NCDO2118 pExu: empty and inflamed groups. This recombinant strain also decreased colonic MPO activity. No difference in the intestinal microbiota was observed between all treatments. Altogether, our results show that recombinant L. lactis NCDO2118 delivering p62 protein protected the intestinal mucosa and mitigated inflammatory damages caused by dextran sodium sulfate (DSS). We thus suggest that p62 may constitute part of a therapeutic approach targeting inflammation.

Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests.

Tissue engineering is vital in treating injuries and restoring damaged tissues, aiming to accelerate regeneration and optimize the complex healing process. In this study, multizonal scaffolds, designed to mimic tissues with bilayer architecture, were prepared using the rotary jet spinning technique (RJS scaffolds). Polycaprolactone and different concentrations of alginate hydrogel (2, 4, and 6% m/v) were used. The materials were swollen in pracaxi vegetable oil (PO) (Pentaclethra macroloba) and evaluated in terms of surface morphology, wettability, functional groups, thermal behavior, crystallinity, and cytotoxicity. X-ray diffraction (XRD) showed the disappearance of the diffraction peak 2θ = 31.5° for samples from the polycaprolactone/pracaxi/alginate (PCLOA) group, suggesting a reduction of crystallinity according to the presence of PO and semi-crystalline structure. Wettability gradients (0 to 80.91°) were observed according to the deposition layer and hydrogel content. Pore diameters varied between 9.27 µm and 37.57 µm. Molecular interactions with the constituents of the formulation were observed via infrared spectra with Fourier transform (FTIR), and their influence was detected in the reduction of the maximum degradation temperature within the groups of scaffolds (polycaprolactone/alginate (PCLA) and PCLOA) about the control. In vitro tests indicated reduced cell viability in the presence of alginate hydrogel and PO, respectively.

Aplicabilidade de sistema reator anaeróbio compartimentado seguida de filtro anaeróbio no tratamento de efluentes de suinocultura de pequeno porte / Applicability of compartmentalized anaerobic reactor system followed by anaerobic filter on small swine wastewater treatment

RESUMO O objetivo deste trabalho foi avaliar o desempenho de um sistema composto de reator anaeróbio compartimentado (RAC) seguido por filtro anaeróbio (FA), tratando águas residuárias de uma granja suinícola de pequeno porte. Foram analisados os parâmetros: pH, ácidos voláteis totais (AVT), demanda química de oxigênio total (DQOt), demanda química de oxigênio dissolvida (DQOdiss), demanda química de oxigênio devida aos sólidos suspensos (DQOSS), sólidos suspensos totais (SST), sólidos suspensos voláteis (SSV) e nitrogênio total kjeldhal (NTK). Os valores efluentes no RAC e no FA, de DQOt, SST e NTK, foram 6.633 e 4.361 mg.L-1, 2.418 e 595 mg.L-1 e 888 e 954 mg.L-1, respectivamente, para uma carga orgânica volumétrica (COV) de 7,01 kgDQO(m3.d)-1 no RAC e 28,3 kgDQO(m3.d)-1 no FA, e tempo de detenção hidráulico de 70,5 e 17,8 h no RAC e no FA, respectivamente. O sistema RAC seguido de FA mostrou eficiência de remoção média de 88,8% para DQOt e 80,5% de SST. Conclui-se que o sistema de tratamento anaeróbio, composto por RAC seguido de FA, foi eficiente na remoção de DQO e SST, possibilitando, nas condições operacionais impostas, alcançar valores médios de remoção acima de 80%, tornando-se uma ótima alternativa de tratamento de efluentes de suinocultura para granjas de pequeno porte.

ABSTRACT The aim of this work was to assess the performance of a system composed by a compartmentalized anaerobic reactor (CAR) followed by anaerobic filter (AF) in the treatment of the wastewater of as mall pigfarm.The following parameters were analyzed: pH, total volatilefatty acids (VFA), total demand of chemical oxygen (DCOt), dissolved chemical oxygen demand (CODdiss), chemical oxygen demand due to suspended solids (CODSS), total suspended solids (TSS), volatile suspended solids (VSS) and total kjeldhal nitrogen (TKN). The effluents and AF values for the CAR, the DCOt, TSS and TKN were 6,633 and 4,361mg.L-1, 2,418 and 595 mg.L-1, and 888 and 954 mg.L-1, respectively, for a volumetric organic load (VOL) of 7.01 kgDCO(m3.d)-1 in CAR and 28.3 kgDCO(m3.d)-1 in AF, and hydraulic detention time of 70.5 hand 17.8 hin CAR and AF, respectively. The CAR system followed by FA presented a mean removal efficiency of 88.8% for COD and 80.5% for TSS. It is concluded that the anaerobic treatment system consisting of CAR followed by AF was efficient in removing DCO and TSS, allowing with the imposed operating conditions to achieve average removal values above 80%, becoming a great alternative for wastewater treatment in small swinefarms.