Healthy Eating Score-7 as a Measure of Diet Quality in a Military Population.

OBJECTIVE: Identify potential revisions to the Healthy Eating Score (HES-5) that improve associations with the Healthy Eating Index (HEI) -2015 total and component scores. METHODS: Pearson r correlations were determined from soldiers' (n = 433) survey data, including the HES, proposed additional questions, and the Block Food Frequency Questionnaire. RESULTS: Adding sugar-sweetened beverages and energy drink questions (HES-7) strengthened the HES and HEI-2015 correlation (HES-5; r = 0.42, n = 433, r2 = 0.18, P < 0.001) (HES-7; r = 0.52, r2 = 0.27, P < 0.001). The HES components and Block Food Frequency Questionnaire consumption correlations were as follows: quantity of fruit (r = 0.37, r2 = 0.14, P < 0.001), vegetables (r = 0.41, r2 = 0.17, P < 0.001), whole grains (r = 0.35, r2 = 0.12 P < 0.001), dairy (r = 0.34, r2 = 0.12, P < 0.001), fish (r = 0.31, r2 = 0.10, P < 0.001), and energy drink (r = 0.59, r2 = 0.35, P < 0.001). CONCLUSIONS AND IMPLICATIONS: HES-7 had the strongest correlation with HEI-2015. Future studies can explore if including consumption quantity in the HES improves its representation of diet quality.

Bioengineered lungs generated from human iPSCs-derived epithelial cells on native extracellular matrix.

The development of an alternative source for donor lungs would change the paradigm of lung transplantation. Recent studies have demonstrated the potential feasibility of using decellularized lungs as scaffolds for lung tissue regeneration and subsequent implantation. However, finding a reliable cell source and the ability to scale up for recellularization of the lung scaffold still remain significant challenges. To explore the possibility of regeneration of human lung tissue from stem cells in vitro, populations of lung progenitor cells were generated from human iPSCs. To explore the feasibility of producing engineered lungs from stem cells, we repopulated decellularized human lung and rat lungs with iPSC-derived epithelial progenitor cells. The iPSCs-derived epithelial progenitor cells lined the decellularized human lung and expressed most of the epithelial markers when were cultured in a lung bioreactor system. In decellularized rat lungs, these human-derived cells attach and proliferate in a manner similar to what was observed in the decellularized human lung. Our results suggest that repopulation of lung matrix with iPSC-derived lung epithelial cells may be a viable strategy for human lung regeneration and represents an important early step toward translation of this technology.